Transcriptome and proteome analysis of oviposition- and spermatogenesis-related genes of Diaphorina citri

Diaphorina citri (Kuwayama) (Homoptera: Liviidae) is an important pest of Rutaceae crops. D. citri is the vector of citrus Huanglong disease. This pest often causes outbreaks of citrus Huanglong disease and has a devastating impact on the citrus industry. To date, there has been no systematic study of genes related to oviposition and spermatogenesis in D. citri. Based on the transcriptome and proteome sequencing of D. citri abdominal tissues, genetic information on multiple gene families related to oviposition and spermatogenesis in male and female adults was obtained. The gene and protein expression differences of 16 gene family members related to oviposition and spermatogenesis were analyzed in male and female adults. The transcriptome and proteome sequencing data of the Vg, VgR, and Tssk gene families were analyzed. In this study, we analyzed the differences in the gene and protein expression of 16 gene families related to abdominal oviposition and spermatogenesis in female and male D. citri adults. Some of the data indicated that the differences in abdominal oviposition- and spermatogenesis-related gene expression between male and female adults can affect reproductive behavior. However, how the expression of related genes affects the reproductive behavior of male and female adults, including courtship, mating and oviposition, requires further study. The results provide an important research direction for the control of D. citri by using genetic engineering technology.