尼日利亚伊巴丹市表面健康犬粪便中大肠杆菌延伸谱β-内酰胺酶(ESBL)基因检测

Q4 Biochemistry, Genetics and Molecular Biology

Animal Gene Pub Date : 2022-09-01 DOI:10.1016/j.angen.2022.200133

Olutayo Israel Falodun, Mobolaji Christianah Afolabi, Akeem Ganiyu Rabiu

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引用次数: 0

摘要

大肠杆菌中广谱β-内酰胺酶(ESBL)的发病率引起了恐慌，因为ESBL基因的传播构成了公共卫生风险。本研究旨在通过从尼日利亚伊巴丹市表面健康的狗中分离的大肠杆菌确定抗生素敏感性和携带ESBL blaSHV、blaTEM和blaCTX-M。从狗身上收集新鲜通过的粪便样本(n = 60)，装入无菌样品瓶，并按照标准方法进行处理以分离大肠杆菌。采用聚合酶链反应(PCR)对分离的大肠杆菌进行鉴定，并采用Kirby-Bauer圆盘扩散技术对10种抗生素进行检测。选择产生ESBL表型的大肠杆菌进行ESBL blaSHV、blaCTX-M多重PCR分型。31株大肠杆菌中，对氨苄西林、四环素和甲氧苄啶/磺胺甲恶唑分别耐药10株(32.3%)、9株(29.0%)和7株(22.6%)，多药耐药8株(25.8%)。耐多药大肠杆菌分离株(n = 2/8)至少含有blaSHV、blaTEM和blaCTX-M三种基因中的一种，另一株(n = 1)同时含有3种ESBL基因。显然，健康的狗很可能是ESBL-大肠杆菌的宿主，这种细菌可以通过与宠物亲密接触而传播给宠物主人。

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Detection of extended Spectrum β-lactamase (ESBL) genes in Escherichia coli isolated from fecal samples of apparently healthy dogs in Ibadan, Nigeria

The incidence of Extended Spectrum β-Lactamases (ESBL) in Escherichia coli has generated trepidations because dissemination of ESBL genes constitutes risk to public health. The current study aims to determine antibiotic susceptibility and carriage of ESBL bla_SHV, bla_TEM and bla_CTX-M by E. coli isolated from apparently healthy dogs in Ibadan conurbation, Nigeria.

Freshly passed fecal samples (n = 60) were collected from dogs into sterile sample bottles and processed to isolate E. coli following standard methods. Isolated E. coli were confirmed through Polymerase Chain Reaction (PCR) by means of uidA and tested against 10 antibiotics via Kirby-Bauer disc diffusion technique. Escherichia coli showing phenotypic ESBL production were selected for ESBL bla_SHV, bla_TEM bla_CTX-M genotyping using multiplex PCR.

Of the 31 E. coli isolates confirmed, 10 (32.3%), 9 (29.0%) and 7 (22.6%) were resistant to ampicillin, tetracycline and trimethoprim/sulfamethoxazole, respectively while 8 (25.8%) were multidrug resistant (MDR). Escherichia coli isolates (n = 2/8) that were MDR harboured at least one of bla_SHV, bla_TEM and bla_CTX-M genes while another isolate (n = 1) simultaneously possessed the three ESBL genes. Apparently healthy dogs are likely reservoirs of ESBL- E. coli that can spread to pet owners through close contact with their companion animals.

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Animal Gene Agricultural and Biological Sciences-Insect Science

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期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.