Annales De Biologie Animale Biochimie Biophysique最新文献

{"title":"Importance of exogenous saturated fatty acids during brain development and myelination in mice","authors":"J. Bourre, N. Gozlan-Devillierre, O. Morand, N. Baumann","doi":"10.1051/RND:19790205","DOIUrl":"https://doi.org/10.1051/RND:19790205","url":null,"abstract":"was transported into the brain and incorporated into brain lipids. The labelled stearic acid taken up was partly metabolized in the brain either by elongation or by degradation and in situ resynthesis of fatty acids. The activity of oleic acid and mono-unsaturated chains was detectable. The labelled acids were incorporated into lipids or subcellular particles following characteristic kinetics, which showed a diminution by 24 hrs. When analyzing the evolution of each lipid, the phospholipids followed this pattern, but the cerebrosides and free fatty acids did not. The cerebrosides increased up to 50 hrs, while the free fatty acids remained stable, suggesting a physical binding between membranes and these acids. Myelin lipid radioactivity increased up to 3 days. Most of the activity was found in phospholipids; their fatty acids were labelled in saturated as well as in polyunsaturated homologues. However, sphingolipids, especially cerebrosides, also contained large amounts of radioactivity, mainly found in very long chain fatty acids such as lignoceric acid. The presence of unesterified fatty acids was observed. Unlike other lipids, these molecules were found in constant amounts, expressed in radioactivity per mg myelin lipid. The subcutaneously injected stearic acid was taken up by the brain through the blood-brain-barrier and incorporated into synaptosomal lipids as well as into other brain compartments. Phospholipids were potent acceptors. Moreover, a high level of radioactivity occurred in non-esterified fatty acids. A large amount of radioactivity was measured in isolated neurons and astrocytes. The bloodbrain relationship for stearic acid varied during development. Subcutaneously injected (I1’C) stearic acid was taken up by brain with related changes from birth to maturity. Total lipid radioactivity reached a maximum at 18 days of age and decreased afterwards until adulthood. However, specific radioactivity presented a higher value at 1 day of age and declined from then on. The injected acid was taken up at any age and partly metabolized in the brain either by elongation or by degradation in situ and resynthesis of new fatty acids. It was also desaturated and the oleic acid formed was eventually elongated. The incorporation of labelled stearic acid into brain lipid varied with the age of the injected animal.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"503 1","pages":"173-180"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76509014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Essai de conservation prolongée de gamètes à basse température, sans congélation","authors":"L. Henriet, P. Estermans, B. Cuche, J. Maison","doi":"10.1051/RND:19790305","DOIUrl":"https://doi.org/10.1051/RND:19790305","url":null,"abstract":"Trial of long-term gamete storage at low temperature without freezing. In this study we attempted long-term storage of living cells (particularly gametes) at a temperature lower than 0 OC. Contrary to other methods, avoiding cell disorganization no anti-crystallization agent was used. Preservation was obtained by lowering the freezing point of the cell medium by increasing the pressure. The cells thus treated emerged from the tests apparently intact, but the spermatozoa were not motile.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"4 1","pages":"367-373"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73035555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the digestive microflora of holoxenic * rabbits from birth until adulthood","authors":"P. Gouet, G. Fonty","doi":"10.1051/RND:19790501","DOIUrl":"https://doi.org/10.1051/RND:19790501","url":null,"abstract":"HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Changes in the digestive microflora of holoxenic * rabbits from birth until adulthood Ph. Gouet, G. Fonty","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"3 1","pages":"553-566"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74538746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular steroidogenesis and its regulation in the primate fetus and newborn","authors":"I. Huhtaniemi, C. Korenbrot, P. Lautala, R. Jaffe","doi":"10.1051/RND:19790817","DOIUrl":"https://doi.org/10.1051/RND:19790817","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"20 1","pages":"1327-1338"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74617636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro studies of oocyte maturation and follicular metabolism in the pig","authors":"M. Gérard, Y. Ménézo, P. Rombauts, D. Szöllösi, C. Thibault","doi":"10.1051/RND:19790914","DOIUrl":"https://doi.org/10.1051/RND:19790914","url":null,"abstract":"centrations ; a significant level of progesterone is, however, always noted. A switch of E2/Pg ratio from 2.0 (or higher) to 0.15 occurs after the onset of estrus, when meiosis resumes. In vitro the E2/Pg ratio changes only in follicles of 7.0 mm or larger in diameter when either oFSH or oLH is added to the culture medium ; meiosis always resumes and the granulosa and cumulus cell reactions are similar to that occurring in vivo. During culture of follicles < 6 mm the reduction of the E2/Pg ratio is not observed whatever the nuclear status may be at the end of culture. For the nuclear maturation of oocytes high progesterone concentration thus does not seem to be necessary. No consistent morphological changes of the granulosa and cumulus cells could be associated with meiotic maturation of the oocyte. Glucose consumption increases when gonadotropinsare added to the medium but maximal increase is only observed when meiosis resumes.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"79 1","pages":"1521-1535"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79732937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of type and amount of dietary fat on bile flow and composition in rats","authors":"M. Boquillon, J. Clément, M. Boutillon, M. Chabin","doi":"10.1051/RND:19791009","DOIUrl":"https://doi.org/10.1051/RND:19791009","url":null,"abstract":"Summary. The output of bile and biliary components (biliary salts, phospholipids, cholesterol) during the first hour after bile duct catheterization have been determined in conscious rats fed either a commercial diet or semi-purified diets containing 7 or 20 p. 100 of corn oil or 20 p. 100 of lard or mutton tallow. In those conditions, there was no significant difference in bile or biliary salts output among the various experimental groups. On the contrary, the output of cholesterol and, to a lesser extent, that of phospholipids augmented when the percentage of corn oil in the diet was increased from 7 to 20 p. 100 ; as compared to the former group, the output of these two components was lower in the « lard » and « mutton tallow » groups. Among the groups receiving the high-fat diets, absolute and relative biliary phospholipid concentrations were highest in the lard group, while the absolute biliary cholesterol concentration was identical in the lard and mutton tallow groups but lower than in the « corn oil » group. The relative bile salts concentrations were significantly different in the groups fed the semi-purified diets ; the highest value was found in the mutton tallow and corn oil groups (20 p. 100). The proportions of biliary lecithins, as compared to total phospholipids, were constant in the different groups, but the fatty acid patterns of biliary lecithins varied according to the diet fed. During the 6 hrs that the enterohepatic circulation was interrupted, biliary salts and phospholipid outputs decreased from the beginning of collection to the end, particularly when the initial value was higher ; the decrease of cholesterol output was noticeable only in rats fed corn oil. From these experiments, in which dietary protein content remained constant at 17 p. 100, we concluded that dietary fats generally interfere in the synthesis and secretion of biliary components ; feeding 20 p. 100 of corn oil in the diet particularly led to increased phos- pholipid and cholesterol excretion through the bile. In rats fed a 20 p. 100 lard diet, biliary phospholipid output and concentration increased while the bile flow rate tended to decrease.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"114 1","pages":"1725-1736"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75297352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrolases bound to the intestinal brush border : An example of transmembrane proteins","authors":"S. Maroux, D. Louvard, M. Sémériva, P. Desnuelle","doi":"10.1051/RND:19790614","DOIUrl":"https://doi.org/10.1051/RND:19790614","url":null,"abstract":"hydrophobic domain inserted in the membrane, stabilizing the head at the surface ; (c) a short segment penetrating into the cytoplasm. This type of transmembrane proteins could be implicated in the transfer of any kind of information from one side of the cellular plasma membrane to the other. In particular, the amino acids originating from peptides due to the catalytic activity of the « head » might be directly injected into the carrier system. This mechanism has not yet been experimentally confirmed. However, it would economize energy and explain why amino acids originating from peptides in the lumen were better adsorbed than free amino acids.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"46 1","pages":"787-790"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80982091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of undernutrition during late pregnancy on gluconeogenesis and ketogenesis in twin-pregnant ewes","authors":"C. Rémésy, C. Demigné","doi":"10.1051/RND:19790212","DOIUrl":"https://doi.org/10.1051/RND:19790212","url":null,"abstract":"","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"65 1","pages":"241-250"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74798720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of vitamin D or calcium deficiency on duodenal, jejunal and ileal calcium-binding protein and on plasma calcium and 25-hydroxycholecalciferol levels in the growing pig","authors":"M. Thomasset, A. Pointillart, P. Cuisinier-Gleizes, L. Guéguen, N. Gouhier, A. Subrenat, C. Colin, A. Porteu","doi":"10.1051/RND:19790611","DOIUrl":"https://doi.org/10.1051/RND:19790611","url":null,"abstract":"In vitamin D-deficient pigs the amount of intestinal calcium-binding protein (CaBP) decreased by 33 p. 100 in the proximal duodenum and by 70 p. 100 in the midjejunum ; it was unmeasurable in the distal ileum. Plasma calcium levels declined significantly and plasma 25-hydroxycholecalciferol level was undetectable. The pigs adapted to a calcium-deficient diet by considerably increasing jejunal and ileal CaBP.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"22 1","pages":"769-773"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75505246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of a simulation method for the study of beginning growth in ovarian follicles","authors":"J. Mariana, J. Meyer","doi":"10.1051/RND:19790904","DOIUrl":"https://doi.org/10.1051/RND:19790904","url":null,"abstract":"A study of mathematical models capable of describing the structure of the ovarian follicle population led us to define three important factors (Mariana and Millier,1977) : 1) initiation of growth in small follicles with one layer of cells surrounding the oocyte ; 2) follicular growth by granulosa cell multiplication, increase in oocyte size and formation of the antrum ; 3) follicle disappearance due to atresia or ovulation.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"94 1","pages":"1435-1442"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76428330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}