Annales De Biologie Animale Biochimie Biophysique最新文献_第7页

Purification of rat prolactin : Development of an homologous radioimmunological assay and comparison with the NIAMDD system 大鼠催乳素的纯化:一种同源放射免疫检测方法的建立及其与NIAMDD系统的比较

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19791013

N. Martinat, E. Hall, J. Ravault, M. Dubois

{"title":"Purification of rat prolactin : Development of an homologous radioimmunological assay and comparison with the NIAMDD system","authors":"N. Martinat, E. Hall, J. Ravault, M. Dubois","doi":"10.1051/RND:19791013","DOIUrl":"https://doi.org/10.1051/RND:19791013","url":null,"abstract":"the pituitaries dissected. Using a thorough fractionation procedure, the other pituitary hormones were eliminated. To obtain a PRL preparation with high biological activity, gel filtration on Sephadex G 100 was used. The prepared PRL was studied using physicochemical, biological and immunological methods. From gel filtration data the molecular weight of the PRL thus prepared was approximately 26 000. Using a radioreceptor assay the activity of the preparation was two times higher than the NIAMDD preparation of PRL (NIAMDD-RP,). Immunofluorescence studies revealed that rabbit antiserum raised against this PRL had affinity for the PRL cells in ovine, bovine and rat pituitaries but none for the other types of pituitary cells. A double antibody radioimmunoassay was developed using the above hormone and its antiserum. Standard curves were plotted over the range of 16 to 400 pg. Rat FSH, LH, TSH and GH did not react in this assay nor did plasma proteins. The intra-assay coefficient of variation was 10 p. 100 and the between assay 16 p. 100. The rat prolactin radioimmunoassay kit distributed by the National Institute of Arthritis and Metabolic Diseases (NIAMD) was also examined. Prolactin levels in rat serum measured by the NIAMD-radioimmunoassay were twice those measured by our system. The apparent differences in serum levels of PRL simply reflected the lower immunologic activity of the NIAMD prolactin.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"66 1","pages":"1771-1788"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85248697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Transit digestif chez le rat recevant de la caséine traitée en milieu alcalin 碱性培养基中酪蛋白处理大鼠的消化过程

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790630

B. Possompès, Im Soon Choi Schin, P. Besançon

引用次数: 1

Hydrolyse enzymatique et stabilité en milieu acide des liaisons isopeptidiques de la L-méthionyl-caséine l-甲酰基酪蛋白异肽键的酶水解和酸介质稳定性

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790608

A. Puigserver, L. C. Sen, R. E. Feeney, John R. Whitaker

引用次数: 1

Meiosis in fetal freemartin gonads and in rat fetal ovaries in vitro 胚胎自由卵泡性腺和大鼠胚胎卵巢的减数分裂

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790810

J. Prepin, B. Vigier, A. Jost

{"title":"Meiosis in fetal freemartin gonads and in rat fetal ovaries in vitro","authors":"J. Prepin, B. Vigier, A. Jost","doi":"10.1051/RND:19790810","DOIUrl":"https://doi.org/10.1051/RND:19790810","url":null,"abstract":"In the gonads of male or female calf fetuses and of fetal freemartins, the germ cells may present nuclear aspects resembling those described in other species as the « preleptotene chromosome condensation stage ». The maximum of these aspects occurred between 60 and 80 days post-insemination. The fate of the cells presenting these aspects is not know, and it is not certain that this nuclear stage really is part of meiosis. If one takes as a criterion the more conventionally recognized figures (leptotene, zygotene), meiosis was seen in the three freemartin fetuses aged 77 to 88 days, but the percentage of meiotic cells was low. In fetuses older than 90 days no meiotic cells were present in those gonads in which seminiferous cords developed. In gonads which contained no seminiferous cords, meiotic figures were seen only in three animals under 133 days of age ; in older animals meiosis was not present. The presence of seminiferous cords in the gonad is incompatible with the presence of meiotic germ cells. In every case, the germ cells disappeared from the freemartin gonad after day 186, except for a few which persisted until day 222 because they were located in seminiferous cords. In an attempt to determine whether meiosis could be influenced by various agents, rat fetal ovaries were cultivated in vitro with or without estradiol, an anti-estrogen, progesterone, methyladenine, ram rete fluid or « inhibine » extracted from ram rete fluid. In these preliminary non-quantitative experiments, no definite alterations of meiosis were noted.","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"4 1","pages":"1263-1271"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85259040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Effects of anterior and posterior hypothalamic deafferentation on gonadotrophin and prolactin secretion in ovariectomized ewes under hormonally induced oestrous cycles 下丘脑前、后分离对去卵巢母羊促性腺激素和催乳素分泌的影响

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790709

J. Thiéry, M. Blanc, J. Ravault, J. Pelletier, A. Caraty, C. Lavenet, P. Orgeur, J. Poirier, G. Venier

{"title":"Effects of anterior and posterior hypothalamic deafferentation on gonadotrophin and prolactin secretion in ovariectomized ewes under hormonally induced oestrous cycles","authors":"J. Thiéry, M. Blanc, J. Ravault, J. Pelletier, A. Caraty, C. Lavenet, P. Orgeur, J. Poirier, G. Venier","doi":"10.1051/RND:19790709","DOIUrl":"https://doi.org/10.1051/RND:19790709","url":null,"abstract":"Summary. Oestradiol benzoate (OB), when given to ovariectomized ewes under hormo- nally induced oestrous cycles, caused both negative and positive feedback effects, i. e. decreased FSH levels were followed by an FSH surge 15 to 18 hrs later concomitant with a preovulatory-like LH release. This injection also caused a prolactin surge with a spiking release pattern between 9 to 18 to 30 to 42 hrs later. Basal FSH levels, as well as those obtained after OB injection, progressively increased from December to April. Between December and February, no significant variations in prolactin secretion were detected, while later on (February to April) both basal prolactin levels, and those obtained after OB injection rose significantly. These long-term variations of both FSH and prolactin secretion indicate seasonal effects. After anterior deafferentation of the mediobasal hypothalamus (MBH), FSH levels decreased drastically, but remained detectable. The OB-induced surges were totally sup- pressed in some animals, while in others they showed a very small residual response at the time of the residual LH release. Thus, FSH secretion could be regulated by the same hypo- thalamic hormone as LH secretion, i. e. LH-RH. The tonic and/or cyclic regulation was located in the part of the brain anterior to the cut, probably in the septopreoptic-anterior hypothalamic areas. Residual secretion could be dueto the activity of the mediobasal areas,","PeriodicalId":7885,"journal":{"name":"Annales De Biologie Animale Biochimie Biophysique","volume":"88 1","pages":"1081-1097"},"PeriodicalIF":0.0,"publicationDate":"1979-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84344614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Urea cycling and ammonia absorption in vivo in the digestive tract of the rat 大鼠消化道内尿素循环及氨的体内吸收

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790633

C. Demigné, C. Rémésy

引用次数: 35

Etude du matériel sécrété dans la chambre de régénération d'un appendice locomoteur de Porcellio dilatatus Br. (Crustacea : Isopoda) 扩张瓷运动附属物再生室分泌物质的研究。(甲壳类:等足类)

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/rnd:19791016

G. Noulin, C. Besse, B. Lebourg, J. C. Artault, Th. Bauvais

引用次数: 2

Epididymal sperm maturation in the ram : motility, fertilizing ability and embryonic survival after uterine artificial insemination in the ewe 公羊附睾精子成熟:母羊子宫人工授精后精子活力、受精能力和胚胎存活率

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790505

S. Fournier-delpech, G. Colas, M. Courot, R. Ortavant, G. Brice, C. Cornu, Y. Guérin, Y. Lebreton

引用次数: 73

Differentiation of the fetal gonad of Macaca fascicularis with special reference to the testis 束状猕猴胎儿性腺的分化与睾丸的特殊关系

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790803

D. Dang, J. Fouquet

引用次数: 20

Etude morpho-anatomique du primysium (Mthodes de description et d'valuation) primysium的形态解剖研究(描述和评价方法)

Annales De Biologie Animale Biochimie Biophysique Pub Date : 1979-01-01 DOI: 10.1051/RND:19790101

O. Schmitt, T. Degas, P. Pérot, M. Langlois, B. Dumont

引用次数: 8