Andrologia最新文献

{"title":"Reactive Blue 2 Labels Protamine in Late-Haploid Spermatids and Spermatozoa and Can Be Used for Toxicity Evaluation","authors":"Satoshi Yokota,&nbsp;Tomohiko Wakayama,&nbsp;Hidenobu Miyaso,&nbsp;Kousuke Suga,&nbsp;Masakatsu Fujinoki,&nbsp;Satoru Kaneko,&nbsp;Satoshi Kitajima","doi":"10.1155/2023/7364862","DOIUrl":"10.1155/2023/7364862","url":null,"abstract":"<div>\u0000 <p>Reactive blue 2 (RB2) dye specifically binds to the nuclei of human spermatozoa under weakly alkaline conditions, thereby providing a new method for assessing sperm quality. However, this technique has not yet been applied to other mammalian species, such as well-established rodent models, which would allow evaluation of the male reproductive toxicity of new drug candidates in nonclinical studies. We aimed to evaluate the usefulness of RB2 staining in assessing testicular and epididymal sperm toxicity in mice using a busulfan-induced infertility model. Male C57BL/6J mice were intraperitoneally administered 40 mg/kg of busulfan. After 28 days, the testes and epididymis were collected and stained with RB2 at pH 10. In vitro evaluations were conducted on uncoated glass slides with RB2 mixed with mouse synthetic protamines, protamines extracted from the human spermatozoa or intracellular protein components from somatic cells without protamines. Following peanut agglutinin lectin histochemistry, RB2-positive cells were observed in elongating and elongated spermatids at all stages except for stages IX–XI of the seminiferous epithelium. After busulfan administration, the proportion of RB2-positive germ cells in the seminiferous tubules was significantly decreased, and no RB2-positive spermatozoa were found in the caput epididymis of treated mice. Aggregates were observed in a mixture of RB2 dye (pH 10) and protamines but not in a mixture of intracellular protein components without protamines, and this specificity was lost at a neutral pH. Our study demonstrated that RB2 specifically stains steps 12–16 spermatids, indicating specific binding to the protamines expressed in these spermatids. The RB2 staining technique has potential as a biomarker for male reproductive toxicity, allowing for the rapid visualization of protamination in an animal model commonly used for the evaluation of male reproductive toxicity.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/7364862","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135042454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatial–Temporal Characteristics of Semen Quality in Infertile Men in Wenzhou, China during 2010–2018","authors":"Xuchao Dai,&nbsp;Mengqi Zhang,&nbsp;Gang Chen,&nbsp;Chengshuang Pan,&nbsp;Jiujia Zheng,&nbsp;Qianjin Fei,&nbsp;Hong Huang,&nbsp;Zhigang Wu","doi":"10.1155/2023/7416033","DOIUrl":"10.1155/2023/7416033","url":null,"abstract":"<div>\u0000 <p><i>Background</i>. Declining semen quality has become one of the most serious public health problems worldwide; however, the spatial–temporal characteristics of semen quality of infertile men remain unknown. <i>Objective</i>. To explore spatial–temporal characteristics of semen quality of infertile men in Wenzhou from 2010 to 2018 and thereby provide a scientific basis for development of strategies to enhance male reproductive health. <i>Methods</i>. We obtained 22,978 semen sample data from 2010 to 2018 from the Reproductive Medicine Center and Urology of the First Affiliated Hospital of Wenzhou Medical University in Wenzhou, China. Linear regression analyses and spatial autocorrelation analyses were used to study spatial–temporal characteristics of semen quality parameters. <i>Results</i>. Overall, from 2010 to 2018, mean values for total sperm number and semen volume showed linear increasing trends, and mean values for total sperm motility showed linear decreasing trend (all <i>P</i> &lt; 0.05). Cold spot analyses of progressive sperm motility, total sperm motility, and normal sperm forms were also in main urban area of Wenzhou (Lucheng District, Ouhai District, Longwan District, and Dongtou District) and hot spot were also in nonmain urban area of Wenzhou (mainly including Taishun County, Pingyang County, and Wencheng County). However, hot spot analyses of total sperm number and semen volume were mainly in Dongtou District. <i>Conclusions</i>. The main urban area is the “harder-hit area” of semen quality, while for the islands area is the “high-withdrawal area.” Government departments need to pay more attention to improve male reproductive health in the main urban areas and actively control the potential influencing factors of male reproductive health, such as occupational exposure and life stress. Good environmental quality and plenty of seafood intake may be beneficial to semen quality.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/7416033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135241020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nose Size Predicts Adult Erectile Penile Length","authors":"Chunlu Xu,&nbsp;Tao Song,&nbsp;Yutian Dai,&nbsp;Guohai Sun","doi":"10.1155/2023/6636816","DOIUrl":"10.1155/2023/6636816","url":null,"abstract":"<div>\u0000 <p>Previous studies have proved that nose size has a positive correlation with stretched penile length but stretched penile length does not fully represent erectile penile length. It is considered that the formation and growing ability of the penis is determined by the action of androgen in the fetal period and nose size has also been determined to some extent before birth. If the nose size is indeed related to the length of the penis, there must be a common mechanism behind it. To further confirm the relationship between nose size and penile length, especially erectile penile length, a correlation analysis was carried out by collecting general data and the data related to the nose, ears, and genitals of 377 males aged 20–56 years, including erectile penile length and erectile penile circumference. The results revealed that nose size had the highest correlation with erectile penile length (<i>r</i> = 0.507, <i>p</i> = 0) and flaccid penile length (<i>r</i> = 0.451, <i>p</i> = 0). Therefore, it is believed that nose size is an independent predictor of flaccid and erectile penile length, and the common factors that contribute to this correlation may appear in the fetal period.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/6636816","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135340503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of TAPP Combined with Biological Mesh on Reproductive Function in Male Inguinal Hernia Patients of Reproductive Age: A Single-Center Retrospective Study","authors":"Li-Xiao Zhang,&nbsp;Jia-Qi Kang,&nbsp;Zhi-Bin Ye,&nbsp;Li-Fei Zhang,&nbsp;Hong-Yu Zhao,&nbsp;Jiang-Nan Ge,&nbsp;Wei Liang","doi":"10.1155/2023/5009982","DOIUrl":"10.1155/2023/5009982","url":null,"abstract":"<div>\u0000 <p><i>Objective</i>. To explore the effect of laparoscopic trans-abdominal preperitoneal hernia repair (TAPP) combined with biological mesh on the reproductive function of male inguinal hernia patients during the reproductive period. <i>Methods</i>. Sixty male inguinal hernia patients during the reproductive period admitted to our department from October 2015 to October 2021 were selected for retrospective analysis. All patients were treated with TAPP combined with biological mesh by the same team. Bilateral testicular volume, testicular temperature, testicular blood perfusion, peripheral serum testosterone concentration, and semen analysis were examined before surgery, 3 months after surgery, 6 months after surgery, and 12 months after surgery. <i>Results</i>. The preoperative testicular volume, temperature, and blood perfusion were compared between the healthy side and the affected side; the testicular volume, temperature, blood perfusion of the affected side, peripheral blood testosterone concentration, and semen analysis were compared among the four time points, which were before operation, 3 months after operation, 6 months after operation, and 12 months after operation. All <i>p</i> values were &gt;0.05. <i>Conclusions</i>. The presence of inguinal hernia had no significant effect on the volume and blood perfusion of male testis. In addition, TAPP combined with biological mesh has no negative effect on postoperative reproductive function of male inguinal hernia patients of childbearing age, which manifested as a safe and feasible surgical method.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/5009982","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135871087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison between Microsurgical Varicocelectomy with and without Testicular Delivery for Treatment of Varicocele: An Updated Systematic Review and Meta-Analysis","authors":"Yulong Wang,&nbsp;Yuxuan Song,&nbsp;Caipeng Qin,&nbsp;Chunlong Zhang,&nbsp;Yiqing Du,&nbsp;Tao Xu","doi":"10.1155/2023/7348578","DOIUrl":"10.1155/2023/7348578","url":null,"abstract":"<div>\u0000 <p>Dilated testicular gubernacular veins after varicocelectomy were reported to be the cause of varicocele recurrence. The gubernacular veins start at the tail of the testicular and merges into the posterior scrotal veins. The gubernacular veins could be ligated only after testicular delivery (TD) anatomically. TD and ligation of the gubernacular veins during microsurgical varicocelectomy might reduce the varicocele recurrence but this method is still under debate. Therefore, we performed this systematic review and meta-analysis and assessed varicocele recurrence, complications as well as clinical outcomes after microsurgical varicocelectomy with TD. Relevant researches were searched on the PubMed, Embase, Cochrane Library, Web of Science, and China National Knowledge Infrastructure databases to perform an updated meta-analysis. Standardized mean differences, relative risks (RRs), and their 95% confidence intervals (CIs) were applied to evaluate result indicators. This updated meta-analysis included 17 publications with 10 randomized controlled trials and 7 cohort studies. A total of 2,254 participants, including 1,024 subjects with TD and 1,230 without TD, were involved. The pooled results demonstrated that microsurgical varicocelectomy with TD might lead to higher incidences of postoperative scrotal edema (RR = 4.20, 95% CI = 2.60–6.81, <i>p</i> &lt; 0.001), testicular hydrocele (RR = 6.58, 95% CI = 1.19–36.20, <i>p</i> = 0.030), and orchiepididymitis (RR = 6.33, 95% CI = 2.08–19.31, <i>p</i> = 0.001) than the group without TD. Similar findings were also found in subgroup analysis of varicocele grade II and III. By contrast, we failed to find significant differences between them in semen parameters, varicocele recurrence, serum testosterone, and natural pregnancy (<i>p</i> &gt; 0.05). Sensitivity analysis revealed the pooled results were stable and no publication bias was detected. TD during microsurgical varicocelectomy may lead to more postoperative complications and may not be beneficial for semen parameters, recurrence of varicocele, and natural pregnancy. Therefore, varicocele patients may not benefit more from TD.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/7348578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136023043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorating Effects of Curcumin on Testicular Cancer","authors":"Reza Arefnezhad,&nbsp;Pouya Goleij,&nbsp;Negar Heydari,&nbsp;Fatemeh Rezaei-Tazangi,&nbsp;Hossein Motedayyen","doi":"10.1155/2023/4558299","DOIUrl":"10.1155/2023/4558299","url":null,"abstract":"<div>\u0000 <p>In the young men’s population, testicular cancer (TC) is characterized as one of the main causes of morbidity and mortality around the world. This cancer is mainly divided into germ cell and nongerm cell types and manifests different signs and symptoms, like bone pain, nausea, lower back pain, and anorexia. Also, TC is associated with serious conditions, such as urogenital disorders, infertility, cardiovascular diseases, and solid tumors. Unfortunately, common remedies for TC, including chemotherapy and surgery, have not provided a promising outlook due to their adverse effects on life quality. Ergo, finding a new therapeutic candidate with high effectiveness and low detrimental impacts is indispensable. Newly, herbal remedies have attracted much interest because of their potential to improve diverse diseases, like cancer. In this context, increasing evidence indicated that curcumin, as a polyphenol compound originating from the plant <i>Curcuma longa</i>, has beneficial effects for treating TC. Hence, in this narrative review, we aimed to review documents showing the therapeutic potential of this herbal candidate against TC.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/4558299","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136376472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Number of Available Embryos: A Prediction Tool for Analyzing the Effect of Sperm DNA Fragmentation on Pregnancy Outcome of Patients Undergoing IVF Cycles from Embryology","authors":"Xiaodong Liang,&nbsp;Rubing Duan,&nbsp;Qiangxiang Luo,&nbsp;Haitao Wu,&nbsp;Shasha Si,&nbsp;Ganwen Mo,&nbsp;Peng Ji,&nbsp;Yongbin Liao","doi":"10.1155/2023/1921515","DOIUrl":"10.1155/2023/1921515","url":null,"abstract":"<div>\u0000 <p>Sperm DNA fragmentation (SDF) can affect the pregnancy outcome of assisted reproduction technology. The cumulative live birth rate (CLBR), a new parameter of pregnancy outcome, attracts the attention of researchers to study the effect of SDF on CLBR. This study will focus on whether the number of available embryos (AEN) can be used as a predictor to investigate the effect of SDF on CLBR of in vitro fertilization (IVF) cycles. Our study included 1,347 couples who underwent IVF cycles and detected their SDF results by the sperm chromatin structure assay. Subsequently, CLBR and AEN on Day 3 were examined and compared between the SDF ≤ 30% and SDF &gt; 30% group. The finding by the correlation analysis showed a strong correlation in CLBR and AEN, suggesting that AEN can be used as a predictor of CLBR. When comparing the two groups, significant differences were noticed in CLBR (81.1% vs. 71.5%, <i>P</i> = 0.009) and AEN (8.10 ± 4.43 vs. 7.21 ± 4.27, <i>P</i> = 0.021), revealing that SDF has an influence on both indicators. The results of the covariance analysis suggested that the available embryo rate per maturity oocyte was higher in the SDF ≤ 30% group, and the embryologic parameters showed a significant difference in the available embryo formation rate on Day 3, but not in the fertilization and cleavage rates, suggesting that SDF reduced the quality of embryos, which did not reach the morphological standard of the available embryos, and further reduced AEN and CLBR in the IVF cycles.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/1921515","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134973615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Hedeoma drummondii against Formaldehyde-Induced Testicular Toxicity and Genotoxicity in Wistar Rats","authors":"Nadia Denys Betancourt-Martínez,&nbsp;Pilar Carranza-Rosales,&nbsp;Ezequiel Viveros-Valdez,&nbsp;Nancy Elena Guzmán-Delgado,&nbsp;Maritza Argelia Macias-Corral,&nbsp;Irma Edith Carranza-Torres,&nbsp;Lydia Enith Nava-Rivera,&nbsp;María Soñadora Niño Castañeda,&nbsp;Javier Morán-Martínez","doi":"10.1155/2023/6631345","DOIUrl":"10.1155/2023/6631345","url":null,"abstract":"<div>\u0000 <p>Formaldehyde (FA) is an oxidative stress inducer and a known carcinogen. <i>Hedeoma drummondii</i> has been shown to have potential as a chemopreventive agent. The aim of this study was to assess the protective effect of <i>H. drummondii</i> on FA-induced genotoxic damage, sperm quality parameters, and histological changes of the testis. The male Wistar rats used were divided into the following groups: (G1) 100 mg/kg <i>H. drummondii</i>; (G2) 10 mg/kg FA; (G3) 100 mg/kg <i>H. drummondii</i> + 10 mg/kg FA; (G4) phosphate-buffered saline; (G5) 100 mg/kg <i>α</i>-tocopherol; (G6) 100 mg/kg <i>α</i>-tocopherol + 10 mg/kg FA; and (G7) soybean oil. The results showed a sperm concentration of 23.66 ± 1.52 versus 33.67 cells/mL (<i>p</i> = 0.02), and a percentage of motility of 29.66 ± 1.51 versus 33.33 (<i>p</i> = 0.620), for groups G3 and G6, respectively. Histopathological (H&amp;E) and immunohistochemical analysis of superoxide dismutase showed damage in the germinal epithelium (GE) in G2. While in groups G3 and G6, the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6, <i>p</i> &lt; 0.0001 vs. G2). While in groups G3 and G6 the histological structure was preserved. A protective effect on sperm DNA fragmentation was also observed for treated groups (G3 and G6). In conclusion, FA exposure significantly reduces sperm quality parameters, causes sperm DNA damage, and alters GE in the testis, while <i>H. drummondii</i> has a protective effect against FA-induced sperm genotoxic damage, similar to <i>α</i>-tocopherol.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/6631345","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135729249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of SARS-CoV-2 and Other Upper Respiratory Tract Viral Infections on Reproductive Parameters in Young Healthy Men with Mild Symptoms of Disease","authors":"Angel Elenkov,&nbsp;Anna Nilsson,&nbsp;Alexandra Kondic,&nbsp;Blenda Bottiger,&nbsp;Marcus Broden,&nbsp;Ebba Bergman,&nbsp;Annie Dahl,&nbsp;Aleksander Giwercman","doi":"10.1155/2023/8895384","DOIUrl":"10.1155/2023/8895384","url":null,"abstract":"<div>\u0000 <p>Data regarding excretion of SARS-CoV-2 in semen are inconclusive and counseling regarding risk of sexual transmission is still challenging. Our knowledge on the effect of upper respiratory tract infections (URTI) on male reproductive system is also scarce. Apart from negative effect of fever on spermatogenesis virtually no study has been able to compare reproductive parameters in men with COVID-19 or other URTI with predisease data. Eleven men who developed symptoms of URTI during the first and second wave of COVID-19 pandemic and who had preexistent fertility and hormonal data, participated in the study leaving sperm and blood samples. Three additional subjects were recruited among proven SARS-CoV-2 positive male hospital workers (without previous data). SARS-CoV-2 RNA was present in the ejaculate from 2 of 5 (40%) young men with mild COVID-19. In one of them viral particles could be detected in the semen sample 2 weeks after the first sampling. Men with any URTI showed higher LH (<i>p</i> = 0.02), lower sperm concentration (<i>p</i> = 0.047), and free testosterone (<i>p</i> = 0.008) when compared to their samples delivered 10 years earlier. When SARS-CoV-2 positive subjects were compared to subjects with other URTIs, no difference in levels of reproductive parameters or inflammatory markers was seen. In conclusion, SARS-CoV-2 RNA can be present in the ejaculate but does not seem to affect reproductive parameters any more than other viruses. However, mild URTI was shown to affect the sperm concentration, LH, and free testosterone negatively.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/8895384","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135729353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-DNA Stainability Is Not Related to Embryonic Development and Clinical Outcomes on In Vitro Fertilization Cycles: A Retrospective Study Using a Propensity Score-Matching Analysis","authors":"Mingzhe Song,&nbsp;Wensi Huang,&nbsp;Lijun Ye,&nbsp;Zhiqiang Liu,&nbsp;Weiqiang Xiao,&nbsp;Feng Xiong,&nbsp;Yueqin Peng,&nbsp;Jing Cai,&nbsp;Yong Zeng","doi":"10.1155/2023/1751627","DOIUrl":"10.1155/2023/1751627","url":null,"abstract":"<div>\u0000 <p>The high-DNA stainability (HDS) might be an indicator for the detection of sperm chromatin decondensation structure resulting from the incomplete histone-to-protamine exchange. The aim of our study was to investigate the association of HDS with embryonic development and clinical outcomes after <i>in vitro</i> fertilization (IVF) treatment. Couples underwent IVF cycles from January 2016 to December 2020 were retrospectively studied, including a total of 2,604 target couples undergoing IVF treatment and 628 couples undergoing fresh IVF-embryo transplantation (IVF-ET) treatment. Couples were divided into HDS &gt; 15% group and HDS ≤ 15% group according to HDS levels. After controlling the bias between groups using the propensity score-matching method, data of embryonic development, and clinical outcomes were analyzed. No significant differences were observed between HDS &gt; 15% group and HDS ≤ 15% group regarding fertilization rate (83.33% vs. 84.62%, <i>P</i> = 0.349), two pronuclei rate (81.82% vs. 83.33%, <i>P</i> = 0.613), cleavage rate (100.00% vs. 100.00%, <i>P</i> = 0.172), and high-quality embryo rate (60.00% vs. 60.00%, <i>P</i> = 0.961). Linear regression analysis showed that HDS was negatively associated with fertilization rate (<i>B</i> value = −0.094, 95% CI: −0.184 to −0.005, <i>P</i> = 0.039), whereas no correlation (adjusted <i>B</i> value = −0.081, 95% CI: −0.170 to 0.008, <i>P</i> = 0.074) was observed after adjusting potential confounding factors. The clinical pregnancy rate (62.11% vs. 60.39%, <i>P</i> = 0.699), ongoing pregnancy rate (52.17% vs. 53.10%, <i>P</i> = 0.838), early miscarriage rate (9.94% vs. 7.28%, <i>P</i> = 0.283), late miscarriage rate (0.62% vs. 2.14%, <i>P</i> = 0.305), and live birth rate (51.55% vs. 50.96%, <i>P</i> = 0.838) were not significantly different between groups. Binary logistic regression analysis showed that HDS levels did not affect clinical outcomes after fresh IVF-ET treatments. HDS was not significantly associated with embryonic development and clinical outcomes of IVF. Our findings suggested that HDS evaluation before IVF treatment might be with limited potential to predict embryo development and clinical outcomes.</p>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2023 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2023/1751627","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136078717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}