Andrologia最新文献

{"title":"Varicocele-Induced Male Infertility: A Bibliometric Perspectives of Mechanistic Insights and Clinical Trends","authors":"Dongyue Ma, Dexiu Li, Yinuo Zhang, Sicheng Ma, Guanchao Du, Jun Guo, Fu Wang","doi":"10.1155/and/5003971","DOIUrl":"https://doi.org/10.1155/and/5003971","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Male infertility (MI) has become a global public health challenge, and varicocele (VC) is considered a common cause of male reproductive disorders. Although scientific research on MI has increased in recent years, there is still a significant lack of systematic integration and analysis of related publications.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study employs bibliometric methods to conduct a comprehensive visual analysis of research on MI and VC from 2004 to 2024, aiming to depict the evolutionary trajectory, knowledge framework, and research frontiers in this field.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Tools such as VOSviewer, CiteSpace, Scimago Graphica, Pajek, R-bibliometrix, and R packages were used for bibliometric analysis and visualization of literature retrieved from the Web of Science database.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>From 2004 to 2024, publications on MI and VC have shown exponential growth, with the most pronounced increase occurring in 2020. At the national level, United States and China were the major contributors. Institutionally, the Cleveland Clinic and McGill University emerged as leading research centers. At the author level, Agarwal A. and Esteves S.C. were identified as the most influential contributors. Regarding journals, Andrologia and Fertility and Sterility served as major publication platforms. Keyword analysis delineated the key research domains within MI and VC. High-frequency keywords such as oxidative stress, DNA fragmentation, and spermatogenesis underscored the central research focus on oxidative damage and spermatogenic mechanisms, whereas terms such as varicocelectomy and microsurgery highlighted the sustained clinical interest in surgical interventions. Burst detection suggested a recent shift of research hotspots toward inflammation, lipid peroxidation, obesity, and antioxidant therapies such as vitamin E. Strategic diagram analysis further demonstrated that this field integrates both fundamental and interdisciplinary characteristics. Core topics such as testis and apoptosis were identified, along with well-developed but relatively isolated directions, collectively reflecting a trend of progressive integration from mechanistic exploration to clinical application.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Through bibliometric analysis, this study reveals a significant upward trend in research on MI and VC, reflecting the growing aca","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5003971","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145366299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Apigenin Supplementation in Cryopreservation Medium on Post-Thaw Human Sperm","authors":"Punyawee Utaipatanacheep,&nbsp;Porntip Sirayapiwat,&nbsp;Jiraporn Ngeamvijawat,&nbsp;Natcha Madared,&nbsp;Chanchai Boonla,&nbsp;Nuttanun Panyawongudom","doi":"10.1155/and/6317214","DOIUrl":"https://doi.org/10.1155/and/6317214","url":null,"abstract":"<div>\u0000 \u0000 <section>\u0000 \u0000 <p>Apigenin has been shown to improve post-thaw sperm quality in animal studies; however, data from human studies are limited. This study aimed to investigate the effects of apigenin on the quality of post-thawed human sperm. Semen samples were obtained from 57 healthy participants aged 18–45 years. Prefrozen sperm was evaluated for sperm concentration and motility and then equally divided into two groups: (1) control group, wherein sperm was frozen in standard medium without supplementation and (2) apigenin group, wherein sperm was frozen in medium supplemented with 0.2 mmol/L apigenin. Samples underwent rapid freezing and were stored in liquid nitrogen for at least 1 week. After thawing, sperm motility, viability, total antioxidant capacity (TAC), and oxidative stress markers were compared between the control and apigenin groups. Total motility (27.78% vs. 22.38%, <i>p</i> = 0.007), progressive motility (24.74% vs. 20.82%, <i>p</i> = 0.007), and viability (39.55 ± 13.13% vs. 35.50 ± 13.28%, <i>p</i> = 0.04) were significantly higher in the apigenin group than in the control group. Intracellular reactive oxygen species (ROS) (<i>p</i> = 0.011) and protein carbonyl (<i>p</i> = 0.014) were significantly lower in the apigenin group than in the control group, indicating reduced oxidative stress. No significant differences in TAC and nitric oxide levels were observed between the groups; however, apigenin significantly enhanced post-thaw human sperm quality. This cryopreservation method may be clinically useful for maintaining sperm quality for intrauterine insemination and in vitro fertilization.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Trial Registration</h3>\u0000 \u0000 <p>ClinicalTrials.gov identifier: NCT06560216</p>\u0000 </section>\u0000 </div>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6317214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Caffeic Acid and Chitosan on Spermatological Parameters and Pregnancy Rate in Tuj Breed Ram Semen","authors":"Taygun Gökdemir,&nbsp;Savaş Yıldız","doi":"10.1155/and/6257707","DOIUrl":"https://doi.org/10.1155/and/6257707","url":null,"abstract":"<p>The aim of this study is to determine the effect of caffeic acid and chitosan (CS) added to the sperm diluent on post-thaw spermatological parameters and pregnancy rates in laparoscopic artificial insemination (AI) procedures by utilizing their protective effects. Semen samples were collected from five Tuj rams and diluted using a TRIS-based extender containing different concentrations of caffeic acid (0.018 mg, GA1; 0.009 mg, GA2), CS (0.005 mg, GB1; 0.01 mg, GB2), and a control (C) group without antioxidant supplementation. Post-thaw evaluations included assessments of semen motility, live percent, percent abnormal, and hypo-osmotic swelling test (HOST). Additionally, acrosomal integrity and mitochondrial membrane potential (MMP) were analyzed via flow cytometry. Each treatment group was subjected to laparoscopic intrauterine insemination using 10 estrus-synchronized ewes during the breeding season. Following thawing, the groups supplemented with caffeic acid demonstrated significant improvements (<i>p</i>  &lt; 0.05) in motility, live percent, and lower rates of morphological compared to other groups. Specifically, the GA1 group exhibited a significant positive effect on acrosomal integrity (<i>p</i>  &lt; 0.05). However, the groups containing CS showed detrimental effects on MMP (<i>p</i>  &lt; 0.05). Although a numerically higher pregnancy rate was observed in the GA1 group, the differences were not statistically significant (<i>p</i>  &gt; 0.05). In conclusion, the study contributed novelty by subjecting these two antioxidants to detailed spermatological evaluations and assessing pregnancy outcomes following laparoscopic AI. Although there was no statistically significant difference in pregnancy rates, caffeic acid showed positive effects on sperm quality after dissolution in rams, while CS did not show any improvement compared to other groups.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/6257707","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145272546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Air Pollution on Semen Quality: A Cross-Sectional Study","authors":"Miaomiao Ma,&nbsp;Chenming Zhang,&nbsp;Xiao Li,&nbsp;Junchao Yao,&nbsp;Luyu Li,&nbsp;Yongtao Zhang,&nbsp;Feng Liu,&nbsp;Baojun Ju","doi":"10.1155/and/2211144","DOIUrl":"https://doi.org/10.1155/and/2211144","url":null,"abstract":"<p><b>Background:</b> Conclusions about the effect of air pollution on semen quality are not uniform, and there is a lack of large-scale studies.</p><p><b>Methods:</b> We analyzed 1230 male semen samples and assessed semen quality parameters. Logistic analysis was performed on the air quality indicators and semen quality parameters of the three critical periods of sperm development and the entire exposure period to determine the air pollution with significant differences and to find the sensitive period.</p><p><b>Results:</b> During the 0–90 days exposure period, every unit of PM₁₀ increase was associated with a 98% increase in abnormal semen pH. During the 0–9 day exposure period, every unit of SO₂ increase was associated with an 18% increase in abnormal semen routine and a 38% increase in abnormal semen pH; for each unit of CO increase, the abnormal pH increased by 24%. During the 10–14 days exposure period, every unit increase in PM_2.5 was associated with a 245% increase in abnormal semen volume; each unit increase in SO₂ increased the abnormal semen pH by 40%. During the 70–90 days exposure period, every unit of PM_2.5 increase was associated with a 90% increase in abnormal semen pH.</p><p><b>Conclusion:</b> Air pollution is associated with semen quality and can affect the entire stage of sperm production, with SO₂ and PM_2.5 exposure being particularly impactful. Air pollution is particularly sensitive to sperm storage periods (0–9 days before ejaculation).</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/2211144","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145146956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fuzheng Yiliu Decoction-Medicated Serum Promotes Ferroptosis in Prostate Cancer Cells via PPARγ-PLIN2 Pathway","authors":"Wei Fu,&nbsp;Meixin Lin,&nbsp;Yongting Yan,&nbsp;Sirui Wu,&nbsp;Qixin Li,&nbsp;Zezheng Zhang,&nbsp;Xujun You","doi":"10.1155/and/1728853","DOIUrl":"https://doi.org/10.1155/and/1728853","url":null,"abstract":"<p><b>Background:</b> Prostate cancer (PCa) is the second most prevalent malignancy worldwide, yet effective treatment options remain limited. Fuzheng Yiliu decoction (FZYL), a traditional Chinese medicine (TCM) formula, exhibits anti-tumor properties, its mechanism of action in PCa is poorly understood. This study aimed to elucidate the therapeutic potential and underlying molecular mechanisms of FZYL-medicated serum in PCa treatment.</p><p><b>Methods:</b> FZYL-medicated serum was prepared and applied to PCa cells (DU145 and PC3). Cell proliferation and apoptosis were assessed using cell counting kit-8 (CCK-8), propidium iodide (PI) staining, and TdT-mediated dUTP nick-end labeling (TUNEL) assay. Further the treated PCa cells were used for isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic analysis. Perilipin 2 (pLIN2) and peroxisome proliferator-activated receptor gamma (PPARγ) levels were evaluated via qRT-PCR and western blot. Dual-luciferase reporter gene assay was employed to confirm the binding between pLIN2 and PPARγ. Ferroptosis markers, including reactive oxygen species (ROS), Fe²⁺, glutathione (GSH), and malondialdehyde (MDA) were measured.</p><p><b>Results:</b> FZYL-medicated serum significantly inhibited DU145 and PC3 cell proliferation and induced apoptosis. Proteomic analysis revealed activation of the PPARγ-pLIN2 pathway, with pLIN2 transcription being regulated by PPARγ. Futherover, FZYL-medicated serum enhanced ferroptosis sensitivity, as evidenced by elevated ROSFe²⁺ and MDA levels and reduced GSH level. Overexpression of pLIN2 similarly promoted ferroptosis, confirming the pathway’s involvement.</p><p><b>Conclusions:</b> Our findings demonstrated that FZYL-medicated serum induces ferroptosis in PCa cells through the PPARγ-pLIN2 pathway, providing a novel therapeutic strategy for PCa treatment.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1728853","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Support for the Increased Risk of Erectile Dysfunction in Prostate Cancer: A Bidirectional Mendelian Randomization Study","authors":"Miaoyong Ye,&nbsp;Song Chen,&nbsp;Junwei Wang,&nbsp;Cunming Zhang,&nbsp;Zhongbiao Wu,&nbsp;Jianxiong Ma,&nbsp;Baijun Chen,&nbsp;Fan Zhao","doi":"10.1155/and/8130830","DOIUrl":"https://doi.org/10.1155/and/8130830","url":null,"abstract":"<p>Epidemiological evidence has shown that prostate cancer (PCa) and erectile dysfunction (ED) are factors of increased mutual risk. However, the causal relationship between PCa and ED remains elusive due to the methodological limitations of traditional observational studies. Thus, we performed a bidirectional two-sample Mendelian randomization (MR) study to investigate the causal relationship between PCa and ED. We used genome-wide association studies of PCa and ED to obtain single-nucleotide polymorphisms (SNPs). The inverse variance weighted method was used as the primary analysis tool to obtain causal effects, and we adopted an MR–Egger, weighted median, simple, and weighted model for the complementary analysis. Using univariable MR, genetic evidence indicated that PCa has a causal genetic liability effect on ED using both the explored and the replicate dataset (odds ratio [OR] 1.11, 95% confidence interval [CI] 1.06–1.17, <i>p</i> &lt; 0.001 and OR 1.62, 95% CI 1.48–1.78, <i>p</i> &lt; 0.001, respectively). Furthermore, the combined result of the meta-analysis (inverse variance weighting [IVW]: OR 1.17, 95% CI 1.12–1.23, <i>p</i> &lt; 0.001). In the reverse direction, ED showed no genetic liability on PCa (OR 1.01, 95% CI 0.96–1.06, <i>p</i> = 0.759 and OR 1.17, 95% CI: 0.93–1.48, <i>p</i> = 0.172). In multivariate MR, the association remained after eliminating the confounders to estimate the direct causal effect of PCa on ED (all <i>p</i> &lt; 0.001). This study provided genetic evidence suggesting that PCa has a causal effect on increased ED risk, but ED showed no such effect on PCa.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/8130830","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dexamethasone-Induced Chronic Stress Decreases Biochemical Fluid Components and Seminal Vesicle Secreting Protein 4 Expression Via Epithelial Cell Apoptosis","authors":"Sararat Innoi,&nbsp;Tidarat Chawalchitiporn,&nbsp;Tarinee Sawatpanich,&nbsp;Nareelak Tangsrisakda,&nbsp;Chadaporn Chaimontri,&nbsp;Chayakorn Taoto,&nbsp;Therachon Kamollerd,&nbsp;Yutthaphong Patjorn,&nbsp;Apichaya Wongsomwong,&nbsp;Auekarn Chaiyamart,&nbsp;Wipawee Thukhammee,&nbsp;Chanasorn Poodendaen,&nbsp;Suthat Duangchit,&nbsp;Sitthichai Iamsaard","doi":"10.1155/and/7168333","DOIUrl":"https://doi.org/10.1155/and/7168333","url":null,"abstract":"<p><b>Background:</b> Dexamethasone (DEX) has recently been used to treat inflammation and to induce the chronic stress (CS) in animal models. Although its adverse effects on testicular damage and seminal plasma reduction were previously reported, the changes in the seminal vesicle are still unexplored. This study aimed to investigate the effects of DEX on biochemical alterations in seminal vesicle fluid (SVF) and seminal vesicle tissue (SVT).</p><p><b>Methods:</b> Male rats were divided into control and DEX groups (<i>n</i> = 20). CS animals were induced with DEX at 1.5 mg/KgBW for 21 consecutive days. CS behaviors were confirmed by the tests of tail suspension, forced swimming, and sucrose preference. Hormones and biochemical components in serum and SVF were evaluated. SVF volume and histomorphometry of SVT were observed. Expressions of apoptotic markers and seminal vesicle secreting protein 4 (SVS4) were determined.</p><p><b>Results:</b> The results showed the reductions of seminal vesicle size and SVF volume in DEX rats. DEX increased the malondialdehyde (MDA) level in SVF, and DNA fragmentation revealed by the TUNEL assay. Serum testosterone and levels of magnesium and fructosamine in SVF of the DEX group were significantly decreased. DEX altered the thickness of the seminal vesicle wall. The expression of SVS4 was decreased in SVT induced with DEX. However, no difference in apoptotic expressions (Hsp70, procaspase-3, and procaspase-9) was observed between groups.</p><p><b>Conclusion:</b> The DEX-induced CS caused the seminal vesicle changes, which were associated with DNA fragmentation. Decreases of fructosamine and SVS4 may be a cause of low-sperm quality such as motility and capacitation in CS men.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/7168333","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Wuzi Yanzong Pill on Mitochondrial Homeostasis and the cGAS-STING Signaling Pathway in Rats Under Testicular Heat Stress","authors":"Chenxiao Liu,&nbsp;Zuomei Zeng,&nbsp;Xinyue Wang,&nbsp;Yichen Wang,&nbsp;Di Deng,&nbsp;Jian Guo","doi":"10.1155/and/1637113","DOIUrl":"https://doi.org/10.1155/and/1637113","url":null,"abstract":"<p>The incidence of male infertility has remained notably high in recent years, with testicular inflammation being a major contributing factor. Testicular heat stress (THS) is a common inducer of testicular inflammation. Testicular tissue is rich in mitochondria, and disruptions in mitochondrial homeostasis may play a key role in driving inflammation. Wuzi Yanzong Pill (WZYZ Pill), a traditional Chinese medicine (TCM) formula, has long been used in treating male infertility and may inhibit testicular inflammation caused by heat stress. In this study, a rat model of THS was established, and WZYZ Pill was administered at different doses. The effects of WZYZ Pill on sperm parameters, testicular histology, and the secretion of inflammatory factors were assessed. The study also examined the link between mitochondrial imbalance and inflammation, aiming to clarify whether the WZYZ Pill interferes with this pathway. Western blotting, immunohistochemistry, and real-time PCR (RT-PCR) were used to assess mitochondrial DNA (mtDNA) leakage and the expression of key regulators of mitochondrial homeostasis and the cyclic GMP–AMP synthase (cGAS)-stimulator of interferon gene (STING) signaling pathway. The results showed that heat stress disrupted mitochondrial homeostasis, leading to mtDNA leakage and activation of the cGAS-STING pathway, which contributed to testicular inflammation. WZYZ Pill significantly improved mitochondrial homeostasis, reduced mtDNA leakage, suppressed the expression of cGAS-STING-related molecules, and decreased the secretion of inflammatory factors in testicular tissue, ultimately alleviating the adverse effects of heat stress on both testicular structure and sperm production.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/1637113","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin Attenuates Bisphenol A-Induced Leydig Cell Damage by Activating Autophagy","authors":"Yousef Asadi-Fard,&nbsp;Layasadat Khorsandi,&nbsp;Elham Younesi,&nbsp;Mohammad Javad Khodayar,&nbsp;Abbass Heidari-Moghadam","doi":"10.1155/and/5537141","DOIUrl":"https://doi.org/10.1155/and/5537141","url":null,"abstract":"<p>The toxic effects of bisphenol A (BPA) on the male reproductive system have been shown in previous research. The impact of naringenin (NG) on BPA-induced Leydig cell (TM3 cell line) toxicity and its mechanism was examined in the present study. The TM3 cells were treated with BPA or NG in various groups for 48 h. To analyze the autophagy role of NG and BPA in TM3 cells, the cells were pretreated with 3-methyladenine (3-Ma), an autophagy inhibitor. The viability rate of TM3 cells notably decreased when exposed to the BPA group. BPA induces oxidative stress in TM3 cells by diminishing crucial antioxidant components such as glutathione, superoxide dismutase, catalase, and glutathione peroxidase while simultaneously increasing the generation of reactive oxygen species (ROS). BPA significantly increased the Bax/Bcl-2 ratio and ROS generation while decreasing testosterone concentration. The expression of autophagy-inducer genes (ATG7, ATG5, and Beclin-1) and the ratio of LCSII/LC3I proteins were significantly reduced, while mTOR expression was increased in the BPA group. NG pretreatment decreased the Bax/Bcl-2 ratio and expression of mTOR, while increasing the LC3II/LC3I ratio and the expression of ATG5, ATG7, and Beclin-1 proteins. NG could also reverse testosterone and antioxidant levels of the BPA-intoxicated cells. Treatment with 3-Ma led to a significant decrease in cell viability in TM3 cells upon exposure to NG and BPA, whereas the untreated 3-Ma groups showed higher viability. In the presence of 3-Ma, NG caused a decline in testosterone levels and antioxidant biomarkers while raising the Bax/Bcl-2 ratio and ROS production in TM3 cells exposed to BPA. These results propose that NGs protect TM3 cells from BPA by enhancing autophagy and lowering apoptosis and oxidative stress.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/5537141","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144918640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Wet Cupping With Sperm Quality and Mitochondrial Gene Expression in a Rat Model of Metabolic Male Infertility","authors":"Soha Albeitawi,&nbsp;Mariam Al-Omari,&nbsp;Fatimah Almahasneh,&nbsp;Nesreen Bataineh,&nbsp;Tareq Al-Omari","doi":"10.1155/and/4092625","DOIUrl":"https://doi.org/10.1155/and/4092625","url":null,"abstract":"<p>Infertility is an increasingly common condition associated with significant psychological and economic burdens. Treatments of infertility have limited success rates and come with a high cost and emotional toll, which highlights the need for other therapies. Wet cupping has been investigated for the treatment of different conditions, but not infertility. We therefore investigated the effects of wet cupping in a rat model of metabolic male infertility. Sperm concentration and viability, testicular weight, epididymal weight, Johnson score, and serum testosterone (T) significantly improved after wet cupping (<i>p</i>-value = 0.026, 0.026, 0.006, 0.015, and 0.004, respectively). In addition, wet cupping was associated with an increased expression of NDUF8 and SDHB mitochondrial genes (<i>p</i>-value = 0.041), and <i>TNF-α</i> (<i>p</i>-value = 0.035), while no significant effect was observed on sperm’s morphology and motility, and follicle-stimulating hormone (FSH) and luteinizing hormone (LH) serum levels. Our results suggest that wet cupping can have a potential role in treating male infertility. However, there are several limitations, such as serum inflammatory markers and oxidative stress markers were not measured. Therefore, further studies are required to investigate the related mechanism and to prove any beneficial effects.</p>","PeriodicalId":7817,"journal":{"name":"Andrologia","volume":"2025 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/and/4092625","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144905546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}