Anesthesia & Analgesia最新文献

{"title":"The Effect of Midazolam Induction on Frontal Electroencephalogram Power.","authors":"Gabrielle A White-Dzuro,Amy Du,Emery N Brown,Oluwaseun Akeju,Robert A Peterfreund","doi":"10.1213/ane.0000000000007556","DOIUrl":"https://doi.org/10.1213/ane.0000000000007556","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound Gel versus Saline for Acoustic Coupling in Ultrasound-Guided Regional Anesthesia: A Randomized, Cross-Over, Simulation Study.","authors":"Garrett W Burnett,Amar Bhavsar,Stephanie Hojsak,Christina Jeng,Michael Anderson,Yuxia Ouyang,Hung-Mo Lin,Chang H Park","doi":"10.1213/ane.0000000000007585","DOIUrl":"https://doi.org/10.1213/ane.0000000000007585","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual Orientation and Gender Identity Representation in Academic Anesthesiology Department Chairs: A Cross-Sectional Survey.","authors":"Travis L Reece-Nguyen,Samantha E Pope,Kyle J Sanchez,Faria Nisar,Hannah E Hill,Luis E Tollinche","doi":"10.1213/ane.0000000000007579","DOIUrl":"https://doi.org/10.1213/ane.0000000000007579","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Processed Electroencephalographic Indices Be Used to Estimate Postoperative Delirium Risk?","authors":"Seyed A Safavynia","doi":"10.1213/ane.0000000000007573","DOIUrl":"https://doi.org/10.1213/ane.0000000000007573","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"133 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond Hotspots: Functional Characterization of the Novel p.Asp2730Tyr Mutation in RYR1 Associated With Malignant Hyperthermia.","authors":"Yao Yu,Yang Zhou,Yiyin Zhang,Zhukai Cong,Zexin Tong,Jiechu Wang,Luyang Feng,Tingting Hou,Zhengqian Li,Xiangyang Guo,Yinyin Qu","doi":"10.1213/ane.0000000000007584","DOIUrl":"https://doi.org/10.1213/ane.0000000000007584","url":null,"abstract":"BACKGROUNDMalignant hyperthermia (MH) is a life-threatening pharmacogenetic disorder triggered by certain anesthetics, characterized by muscle rigidity, elevated body temperature, and hypermetabolic crisis. This condition is primarily associated with genetic mutations in ryanodine receptor 1 (RYR1), which encodes the pivotal calcium release channel in the sarcoplasmic reticulum of skeletal muscle. While numerous hotspot mutations in RYR1 have been identified, the functional impact of nonhotspot mutations on channel activity related to MH remains insufficiently investigated. In this study, we identified a known pathogenic mutation (p.Arg2508His) and a novel variant (p.Asp2730Tyr), both located outside the conventional MH hotspots, in 2 patients with clinical suspicion of MH. Our objective was to investigate the functional implications of the p.Asp2730Tyr mutation in RYR1 on calcium release dynamics related to MH.METHODSWe engineered a recombinant wild-type (WT) plasmid (pcDNA3.1-3Myc-His-RYR1-WT) to express the full-length mouse skeletal muscle RYR1 using seamless multi-fragment cloning techniques. Two RYR1 mutations, p.Arg2508His (used as positive control) and p.Asp2730Tyr, were separately introduced into the WT plasmid, generating 2 mutant constructs (pcDNA3.1-3Myc-His-RYR1-p.Arg2508His and pcDNA3.1-3Myc-His-RYR1-p.Asp2730Tyr). We utilized 293T cells expression system to express either the WT or mutant forms of mouse RYR1. Fluo-4 calcium imaging was conducted to evaluate the alterations in calcium release in response to RYR1 agonists, caffeine or 4-chloro-m-cresol (4CmC), for each mutation compared to WT.RESULTSCells transfected with the p.Arg2508His or p.Asp2730Tyr mutation demonstrated a leftward shift in the caffeine and 4CmC concentration-response curves compared to WT, suggesting an increased channel sensitivity to caffeine and 4CmC (P < .001). The mean ± standard error of the mean (SEM) of the EC50 values for caffeine-induced calcium release was 2.56 ± 0.04 mM in WT, which significantly decreased to 1.32 ± 0.13 mM for p.Arg2508His (P < .001) and 1.12 ± 0.09 mM for p.Asp2730Tyr (P < .001). For 4CmC, the EC50 values were 43.2 ± 1.90 μM in WT, 17.2 ± 0.76 μM for p.Arg2508His (P < .001), and 21.8 ± 1.04 μM for p.Asp2730Tyr (P < .001), indicating enhanced calcium release in both mutations.CONCLUSIONSThe p.Asp2730Tyr mutation, situated beyond the established RYR1 hotspot regions, significantly alters calcium release dynamics related to MH. A comprehensive investigation into the structural conformations, functional assays, and in vivo mechanisms associated with this mutation could yield a more profound understanding of the molecular underpinnings of MH pathogenesis.","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Death in the Dentist's Chair: The Urgent Need for Universal Pediatric Dental Sedation Standards.","authors":"Irim Salik,Kar-Mei Chan,Isabel Pesola,Apolonia E Abramowicz","doi":"10.1213/ane.0000000000007578","DOIUrl":"https://doi.org/10.1213/ane.0000000000007578","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Decision Points for the Functional Fibrinogen Assay of a Novel TEG 6s Heparin Neutralization Cartridge.","authors":"Jan Hartmann,João D Dias,Dieter Adelmann,Hunter B Moore,Kathirvel Subramaniam,Tetsuro Sakai","doi":"10.1213/ane.0000000000007568","DOIUrl":"https://doi.org/10.1213/ane.0000000000007568","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Treadmill Exercise on the Recovery of Synaptic Plasticity in Septic Mice: A Focus on Brain-Derived Neurotrophic Factor/Tropomyosin-Related Kinase B Signaling.","authors":"Takashi Soejima,Koji Hoshino,Yuji Morimoto","doi":"10.1213/ane.0000000000007572","DOIUrl":"https://doi.org/10.1213/ane.0000000000007572","url":null,"abstract":"BACKGROUNDSepsis-associated encephalopathy causes irreversible cognitive dysfunction, yet no effective pharmacological treatments are available. The hippocampus is particularly vulnerable to sepsis-induced damage, and impairments in hippocampal synaptic plasticity, particularly late-phase long-term potentiation (L-LTP), are implicated in cognitive dysfunction. Brain-derived neurotrophic factor (BDNF) and its receptor, tropomyosin receptor kinase B (TrkB), play crucial roles in maintaining L-LTP. While exercise enhances cognitive function, its effects on hippocampal synaptic plasticity under conditions mimicking early rehabilitation after sepsis remain unclear. This study evaluated the impact of treadmill exercise on hippocampal L-LTP in a murine sepsis model, using a protocol resembling early clinical rehabilitation.METHODSA total of 267 C57BL/6J mice (8-12 weeks old) underwent cecal ligation and puncture (CLP) or sham surgery, with or without treadmill exercise (30 min/d for 7 days postsurgery). Mice were divided into 4 groups: (1) sham + sedentary, (2) sham + exercise, (3) CLP + sedentary, and (4) CLP + exercise. The primary outcome was hippocampal L-LTP, assessed via electrophysiology. Secondary outcomes included hippocampal BDNF levels, locomotor activity, and survival curves. Additionally, the role of BDNF/TrkB signaling was examined using ANA-12, an antagonist of the BDNF receptor TrkB. Data are presented as mean ± standard deviation.RESULTSL-LTP at the Schaffer collateral-CA1 synapse was significantly impaired in CLP mice 1 week after surgery (CLP + sedentary: 144% ± 15% vs sham + sedentary: 185% ± 34%; P = .008). Exercise restored L-LTP in CLP mice (CLP + exercise: 189% ± 36% vs CLP + sedentary: 144% ± 15%, P = .003), but this effect was abolished by ANA-12 (CLP + exercise + ANA-12: 155% ± 22% vs CLP + exercise + vehicle: 194% ± 37%, P < .001). Exercise also restored hippocampal BDNF levels reduced by CLP (CLP + exercise: 4190 ± 671 pg/mg protein versus CLP + sedentary: 3220 ± 647 pg/mg protein, P = .007). Locomotor activity was impaired in CLP mice but not significantly improved by exercise (P = .38). Furthermore, the survival curves differed significantly between CLP mice with and without treadmill exercise, as determined by post hoc analysis after a log-rank test (P = .003).CONCLUSIONSTreadmill exercise therapy restored hippocampal L-LTP impaired by sepsis, at least partially mediated by activation of the BDNF/TrkB signaling pathway. Additionally, exercise altered the survival curve, though it had limited effects on locomotor activity. These findings suggest that exercise therapy may mitigate sepsis-induced synaptic dysfunction.","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144130763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Association Between Intraoperative Fluid Management, Blood Pressure, and Patients' Outcome After Complex Spine Surgeries.","authors":"Srikiran Ramarapu,William Daniel Satterwhite","doi":"10.1213/ane.0000000000007566","DOIUrl":"https://doi.org/10.1213/ane.0000000000007566","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perioperative Monitoring: Beyond Arterial Blood Pressure and Beyond the Operating Room.","authors":"Lee A Goeddel,Karsten Bartels","doi":"10.1213/ane.0000000000007534","DOIUrl":"https://doi.org/10.1213/ane.0000000000007534","url":null,"abstract":"","PeriodicalId":7799,"journal":{"name":"Anesthesia & Analgesia","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}