Journal of free radicals in biology & medicine最新文献_第3页

Endogenous antioxidant defenses in neonates 新生儿的内源性抗氧化防御

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80013-7

Warren Rosenfeld, Luzminda Concepcion

引用次数: 8

Damage to biological tissues induced by radical initiator 2,2′-azobis(2-amidinopropane) dihydrochloride and its inhibition by chain-breaking antioxidants 自由基引发剂2,2′-偶氮双(2-氨基丙烷)二盐酸盐对生物组织的损伤及断链抗氧化剂的抑制作用

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80070-8

Kiyoshi Terao , Etsuo Niki

引用次数: 117

Superoxide production and chemiluminescence induced in differentiated HL-60 cells by the chemoattractant formyl-methionyl-leucyl-phenylalanine 甲酰基-甲硫基-亮基-苯丙氨酸诱导分化HL-60细胞产生超氧化物和化学发光

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/0748-5514(86)90119-4

Claes Dahlgren, Tommy Andersson, Olle Stendahl

{"title":"Superoxide production and chemiluminescence induced in differentiated HL-60 cells by the chemoattractant formyl-methionyl-leucyl-phenylalanine","authors":"Claes Dahlgren, Tommy Andersson, Olle Stendahl","doi":"10.1016/0748-5514(86)90119-4","DOIUrl":"10.1016/0748-5514(86)90119-4","url":null,"abstract":"<div><p>Superoxide production and chemiluminescence induced in differentiated HL-60 cells by the chemoatractant formylmethionyl-leucyl-phenylalanine: In order to study the generation of oxidative metabolites in relation to cell differentiation, dimethyl sulfoxide (DMSO) and retinoic acid (RA) differentiated HL-60 cells were stimulated with the chemotactic peptide formylmethionly-leucyl-phenylalanine (FMLP). The oxidative response was measured as luminol-dependent chemiluminescence, lucigenin-dependent chemiluminescence, and cytochrome c reduction. Cells grown in the presence of DMSO or RA progressively expressed morphological changes, and when the mature cells were exposed to FMLP the cells produced oxidative metabolites. Quantitatively the HL-60 cells grown in the presence of DMSO gave rise to the most pronounced response. No correlation was obtained between superoxide production, luminol-chemiluminescence and lucigenin-dependent chemiluminescence, indicating that different aspects of the oxidative response are elucidated by the three different methods. Futhermore, the experiments show that DMSO and RA-induced differentiation of HL-60 cells leads to granulocyte-like cells with different abilities to produce oxidative metabolite, possibly due to differences in receptor function.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"2 1","pages":"Pages 19-24"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0748-5514(86)90119-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14154114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Photosensitization by antitumor agents 3: spectroscopic evidence for superoxide and hydroxyl radical production by anthrapyrazole-sensitized oxidation of nadh 抗肿瘤药物的光敏作用3:蒽吡唑致敏氧化nadh产生超氧化物和羟基自由基的光谱证据

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80009-5

Krzysztof Reszka, Pawel Kolodziejczyk, J. William Lown

引用次数: 21

Announcements and calendar 公告和日历

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80044-7

引用次数: 0

Scavenging of chlorpromazine cation radical by ascorbic acid or glutathione 抗坏血酸或谷胱甘肽对氯丙嗪阳离子自由基的清除作用

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80008-3

Masayasu Bando , Hajime Obazawa , Tetsuji Tanikawa

{"title":"Scavenging of chlorpromazine cation radical by ascorbic acid or glutathione","authors":"Masayasu Bando , Hajime Obazawa , Tetsuji Tanikawa","doi":"10.1016/S0748-5514(86)80008-3","DOIUrl":"10.1016/S0748-5514(86)80008-3","url":null,"abstract":"<div><p>The report presented here demonstrates that scavenging of chlorpromazine cation radical (an absorption maximum = 530 nm) by ascorbic acid or glutathione can be kinetically and stoichiometrically analyzed at pH 1.5 but not at pH 3.0 and 6.0 using a conventional absorption spectrophotometer. The cation radical decays spontaneously about 10 and 200 times faster at pH 3.0 and 6.0, respectively, than at pH 1.5.</p><p>At pH 1.5, ascorbic acid scavenges the cation radical faster than glutathione does, and the following different scavenging mechanisms are postulated from the above kinetic and stoichiometric analysis. The reaction of the cation radical with ascorbic acid is second order. The ascorbic acid free radical, which decays mainly by dismutation, is generated by the bimolecular reaction. In the case of glutathione, on the other hand, about 70% of the scavenged cation radical disappears through free radical chain reactions that glutathione thiol anion and glutathione free radical probably initiate. The remaining (about 30%) disappears by conjugation with glutathione. It may be due to relative nonreactivity of ascorbic acid free radical that free radical chain reactions, found commonly in radical chemistry, do not occur in the scavenging reaction by ascorbic acid.</p><p>Based on the above results, the physiological scavenging mechanisms of the cation radical by the two reducing substances are discussed briefly.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"2 4","pages":"Pages 261-266"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0748-5514(86)80008-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14706370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Influence of antioxidant enzymes in reduction of optic disc edema in experimental optic neuritis 抗氧化酶对实验性视神经炎视盘水肿的影响

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80035-6

John Guy , E. Ann Ellis , G.M. Hope , Narsing A. Rao

引用次数: 24

Visible chemiluminescence from rat brain homogenates undergoing autoxidation. I. Effect of additives and products accumulation 自氧化大鼠脑匀浆的可见化学发光。一、添加剂和产品积累的影响

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/0748-5514(86)90125-X

E.A. Lissi , T. Cáceres , L.A. Videla

引用次数: 65

Safety evaluation of free radical scavengers peg-catalase and peg-superoxide dismutase 自由基清除剂聚乙二醇过氧化氢酶和聚乙二醇超氧化物歧化酶的安全性评价

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80011-3

Anna T. Viau, Abraham Abuchowski, Sylvia Greenspan, Frank F. Davis

{"title":"Safety evaluation of free radical scavengers peg-catalase and peg-superoxide dismutase","authors":"Anna T. Viau, Abraham Abuchowski, Sylvia Greenspan, Frank F. Davis","doi":"10.1016/S0748-5514(86)80011-3","DOIUrl":"10.1016/S0748-5514(86)80011-3","url":null,"abstract":"<div><p>Treatment with catalase and SOD (superoxide dismutase) could diminish the damage due to oxygen free radical formation, but these enzymes are rapidly removed from circulation. The covalent attachment of monomethoxypolyethylene glycol (PEG) to catalase and SOD extended their plasma half-lives. Toxicity of PEG-catalase and PEG-SOD was evaluated in mice and rats prior to their use as free radical scavengers. Rodents used in acute, subacute, and subchronic toxicologic studies could tolerate large doses of PEG-catalase and PEG-SOD without developing toxic signs. The conjugates did not affect survival rate, appearance, behavior, food intake, blood chemistry, hematology, or urinalysis. In general, body weight gains, organ weights, and histomorphology were also unaffected. Massive doses of PEG-catalase caused slight weight loss, splenic hypertrophy, and generalized splenic stimulation in mice. Massive doses of PEG-SOD resulted in vacuolation in splenic macrophages in rats. PEG-catalase and PEG-SOD circulated for 3 days and 8 days, respectively, in mice following i.v. or i.m. administration.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"2 4","pages":"Pages 283-288"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0748-5514(86)80011-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14707021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Prevention of oxygen toxicity with superoxide dismutase and catalase in premature lambs 超氧化物歧化酶和过氧化氢酶预防早产羔羊氧毒性的研究

Journal of free radicals in biology & medicine Pub Date : 1986-01-01 DOI: 10.1016/S0748-5514(86)80012-5

Frans J. Walther , Corrie E.M. Gidding , Irene M. Kuipers , Dirk Willebrand , Edouard M. Bevers , Abraham Abuchowski , Anna T. Viau

{"title":"Prevention of oxygen toxicity with superoxide dismutase and catalase in premature lambs","authors":"Frans J. Walther , Corrie E.M. Gidding , Irene M. Kuipers , Dirk Willebrand , Edouard M. Bevers , Abraham Abuchowski , Anna T. Viau","doi":"10.1016/S0748-5514(86)80012-5","DOIUrl":"10.1016/S0748-5514(86)80012-5","url":null,"abstract":"<div><p>The use of high oxygen concentrations and high mean airway pressures during mechanical ventilation of premature newborn infants with respiratory distress syndrome leads in 20%–30% of the survivors to chronic lung disease. This study explores if exogenous polyethylene glycol conjugated superoxide dismutase (PEG-SOD) and catalase (PEG-CAT) mitigate oxygen toxicity in premature lambs with respiratory distress syndrome. Six pairs of premature lambs were delivered by cesarean section and treated by tracheal instillation of 60 mg natural sheep surfactant/kg/body weight. After birth, all lambs were ventilated with 100% oxygen, and one of each pair received a single intravenous injection of 1 million U/kg PEG-CAT and 50,000 U/kg PEG-SOD. At 8 h of age or after respiratory failure was established, the lambs were killed and the lungs were removed intact. Lung damage was assessed by microscopy. The arterial blood gases, pH, and mean airway pressures of the lambs treated with PEG-SOD/PEG-CAT did not differ from those of the controls. Mean PaO<sub>2</sub> was > 140 mmHg during the first 4 h of the experiments. In the lambs treated with PEG-SOD/PEG-CAT, SOD and CAT levels were very high during the study period and less bronchiolar epithelial damage and lung hemorrhages were found at microscopy.</p></div>","PeriodicalId":77737,"journal":{"name":"Journal of free radicals in biology & medicine","volume":"2 4","pages":"Pages 289-293"},"PeriodicalIF":0.0,"publicationDate":"1986-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0748-5514(86)80012-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14240325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0