Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M最新文献_第2页

Analyzing of ID50EAL data for the standardization of German cockroach allergen extracts in the U.S. 美国德国蟑螂过敏原提取物标准化ID50EAL数据分析

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Robert James, Herman Mitchell, Peter J Gergen, Peyton A Eggleston, Jay E Slater

引用次数: 0

Clinical trials with allergen products: in search of biological markers of efficacy. 过敏原产品的临床试验:寻找疗效的生物学标记。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Philippe Moingeon

{"title":"Clinical trials with allergen products: in search of biological markers of efficacy.","authors":"Philippe Moingeon","doi":"","DOIUrl":"","url":null,"abstract":"I discuss herein our efforts to identify biological markers of efficacy in support of the development of sublingual allergy vaccines. Biomarkers are of major interest to facilitate clinical development, for example by predicting safety and efficacy of candidate vaccines or their components (e.g. adjuvants and formulations) on the basis of immunogenicity evaluated in humans. They will be mandatory in the future to evaluate customized recombinant allergy vaccines designed upon component-resolved diagnosis. In this regard, they must ideally be both qualitative and quantitative. Such markers would also be useful to confirm foreseen mechanisms of action potentially associated with successful immunotherapy (e.g. the Treg hypothesis). The recent availability of sophisticated technologies (referred here as the technology push) to assess in details both humoral and cellular arms of the immune system provides new opportunities to identify such markers. In this regard, documenting natural immune responses, most particularly allergen-specific T cell responses in healthy persons, is critical to identify immunological correlates of protection, and thus to design optimal allergy vaccines.","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"182-7; discussion 187-8"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Recent advances in the use of genetically engineered negative signaling molecules to treat allergic diseases. 利用基因工程负信号分子治疗过敏性疾病的最新进展。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Andrew Saxon, Daocheng Zhu, Ke Zhang, Lisa A Chan, Christopher L Kepley

{"title":"Recent advances in the use of genetically engineered negative signaling molecules to treat allergic diseases.","authors":"Andrew Saxon, Daocheng Zhu, Ke Zhang, Lisa A Chan, Christopher L Kepley","doi":"","DOIUrl":"","url":null,"abstract":"Purpose of review: This review summarizes current knowledge regarding the control of human mast cell and basophil signaling and recent developments using a new therapeutic platform consisting of a human bifunctional gamma and epsilon heavy chain (Fcgamma-Fcepsilon) protein to inhibit allergic reactivity.Recent findings: Crosslinking of FcgammaRIIb to FcepsilonRI on human mast cells and basophils by a genetically engineered Fcgamma-Fcepsilon protein (GE2) leads to the inhibition of mediator release upon FcepsilonRI challenge. GE2 protein was shown to inhibit cord blood-derived mast cell and peripheral blood basophil mediator release in vitro in a dose dependent fashion including inhibition of human IgE reactivity to cat. In addition, IgE-mediated release from lung tissue was inhibited through GE2. The mechanism of inhibition in mast cells included alterations in IgE-mediated Ca2+ mobilization, Syk phosphorylation and the formation of Dok-Grb2-SHIP complex. Proallergic effects of Langerhans-like dendritic cells and B cell IgE switching were also inhibited by GE2. In vivo, GE2 was shown to block passive cutaneous anaphylaxis (PCA) driven by human IgE in mice expressing the human FcepsilonRI and inhibit skin test reactivity to dust mite antigen in a dose dependent manner in rhesus monkeys. The balance between positive and negative signaling controls mast cell and basophil reactivity that is critical in the expression of human allergic diseases. This approach using a human Fcgamma-Fcepsilon fusion protein to co-aggregate FcepsilonRI with the FcgammaRII holds promise as a new therapeutic platform for the immunomodulation of allergic diseases and potentially other mast cell/basophil-dependent disease states.","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"223-31; discussion 231-3"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26631725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Validation of quantitative, allergen-specific ELISAs. 定量、过敏原特异性elisa的验证。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Wolf-Meinhard Becker, Ronald van Ree, Helmut Fiebig, Oliver Cromwell, Bernhard Weber, Rafael Monsalve, Domingo Barber, Monika Raulf-Heimsoth, Philippe Moingeon, Alain Didierlaurent, Gabriella di Felice, Carlo Pini, Kay Fötisch, Stefan Vieths

引用次数: 0

Standardization of animal epithelia. 动物上皮标准化。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Enrique Fernández-Caldas, Jerónimo Carnés, Mayte Gallego, Adriano Mari, Juan Antonio Pagán Alemán

{"title":"Standardization of animal epithelia.","authors":"Enrique Fernández-Caldas, Jerónimo Carnés, Mayte Gallego, Adriano Mari, Juan Antonio Pagán Alemán","doi":"","DOIUrl":"","url":null,"abstract":"The standardization of animal epithelia is warranted for an accurate diagnosis and safe and efficacious treatment of allergic respiratory diseases induced by the inhalation of mammalian aeroallergens. We have compared several sources of raw materials of cat, hamster, goat and rabbit hair and epithelia to establish differences between the protein and allergenic composition of these extracts. The main differences in these raw materials was that \"epithelia\" were supplied as a mixture of hair and epithelia previously treated with acetone, and that the hair was supplied and used untreated. A possible influence of the age of rabbits on the composition of rabbit hair extracts was also evaluated. Overall, important differences were detected in the composition of epithelia versus hair extracts. Epithelia extracts contained more irrelevant proteins and, in most cases, less amounts of major allergens. Important differences were also detected in the composition of the extracts prepared with hair of young versus adult animals. In general, the extracts derived from young animals contained less major allergen and more albumin than those derived from older animals. A greater effort should be made to identify the ideal sources of animal skin derived allergen extracts to provide the allergologists with better extracts for the diagnosis and treatment of allergic respiratory diseases. There is also a need for a consensus on the terminology applied to animal skin derived extracts. The use of the term dander extracts seems to be more appropriate. These extracts contain more relevant allergens and fewer albumins.","PeriodicalId":77490,"journal":{"name":"Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M","volume":" 95","pages":"107-16; discussion 116, 155"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26233184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical evaluation of recombinant allergens. 重组过敏原的临床评价。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Oliver Cromwell, Annemie Narkus

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Recombinant and modified allergens: the U.S. perspective. 重组和改良过敏原:美国的观点。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Ronald L Rabin

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A bright future for sublingual immunotherapy?--pro. 舌下免疫疗法的光明前景?

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Jean Bousquet

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The CREATE project: an introduction. CREATE项目:介绍。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Ronald van Ree

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Multiplex technology for allergen detection and immunodiagnostics. 过敏原检测和免疫诊断的多重技术。

Arbeiten aus dem Paul-Ehrlich-Institut (Bundesamt fur Sera und Impfstoffe) zu Frankfurt a.M Pub Date : 2006-01-01

Martin D Chapman, Amy Tsay, Branka Saric, Rebecca Godbout, Kerry G Oliver

引用次数: 0