Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease最新文献

Deletion of the 19kDa antigen does not alter the protective efficacy of BCG 19kDa抗原的缺失不会改变卡介苗的保护作用

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-12-01 DOI: 10.1054/tuld.2000.0252

V.V. Yeremeev , G.R. Stewart , O. Neyrolles , K. Skrabal , V.G. Avdienko , A.S. Apt , D.B. Young

引用次数: 29

The US–Japan Cooperative Medical Science Program Tuberculosis and Leprosy Panel's 35th Annual Research Conference, Yokohama, Japan: 19–1 July 2000 2000年7月19日至1日，日本横滨，美日合作医学计划结核病和麻风病小组第35届年度研究会议

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-12-01 DOI: 10.1054/tuld.2000.0277

引用次数: 0

CD95 signaling is not required for the down regulation of cellular responses to systemic Mycobacterium tuberculosis infection CD95信号不是下调对系统性结核分枝杆菌感染的细胞反应所必需的

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-12-01 DOI: 10.1054/tuld.2000.0274

J.E. Pearl, I.M. Orme, A.M. Cooper

引用次数: 4

Cytokine transcripts in pediatric tuberculosis: a study with bronchoalveolar cells 儿童结核病的细胞因子转录:支气管肺泡细胞的研究

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-12-01 DOI: 10.1054/tuld.2000.0259

E.M. Aubert-Pivert , F.M. Chedevergne , G.M. Lopez-Ramirez , J.H. Colle , P.L. Scheinmann , B.M. Gicquel , J.M. de Blic

{"title":"Cytokine transcripts in pediatric tuberculosis: a study with bronchoalveolar cells","authors":"E.M. Aubert-Pivert , F.M. Chedevergne , G.M. Lopez-Ramirez , J.H. Colle , P.L. Scheinmann , B.M. Gicquel , J.M. de Blic","doi":"10.1054/tuld.2000.0259","DOIUrl":"10.1054/tuld.2000.0259","url":null,"abstract":"<div>Pediatric tuberculosis (TB) differs from adult TB in many features. To date, cytokine expression has not been studied in children with TB. The relative amounts of the various cytokines released at the site of infection may be important determinants of TB disease development and pathology. We determined cytokine transcripts in bronchoalveolar cells (BACs) recovered from 9 children presenting with TB and from 9 children with pulmonary diseases other than TB. An RT-PCR-based method was developed to quantify the mRNAs encoding six cytokines (IFN- γ, IL-12, TNF- α, IL-10, IL-4, TGF-β 1) known to play key roles in mycobacterial infections. Expression of mRNA encoding TGF- β, TNF-α and IFN- γ was statistically significantly higher in BACs from children with TB than in BACs from children with other pulmonary diseases; whereas the levels of mRNA transcription for TGF- β is high, the levels of mRNA transcription for IFN- γ and TNF- α remain low. All children had low levels of mRNA for IL-12(p40). IL-4 was barely detectable in all cases. Children with miliary TB had high levels of IL-10 transcripts and low levels of mRNA encoding TGF- β. The immunosuppressive cytokines TGF- β and IL-10, are overproduced in children with non-miliary TB and miliary TB respectively and are probably involved in the progression of the disease. These data suggest that Th1 responses are reduced in children with TB.</div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"80 6","pages":"Pages 249-258"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.2000.0259","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81856811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Intracellular passage within macrophages affects the trafficking of virulent tubercle bacilli upon reinfection of other macrophages in a serum-dependent manner 巨噬细胞内的细胞传代以血清依赖的方式影响毒性结核杆菌在其他巨噬细胞再感染时的运输

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-12-01 DOI: 10.1054/tuld.2000.0268

K.A. McDonough , M.A. Florczyk , Y. Kress

{"title":"Intracellular passage within macrophages affects the trafficking of virulent tubercle bacilli upon reinfection of other macrophages in a serum-dependent manner","authors":"K.A. McDonough , M.A. Florczyk , Y. Kress","doi":"10.1054/tuld.2000.0268","DOIUrl":"10.1054/tuld.2000.0268","url":null,"abstract":"<div>Setting: The interaction of tubercle bacilli with macrophages is central to understanding of tuberculosis disease.Objective: The objective was to determine whether prior passage within macrophages affects the behavior of Mycobacterium tuberculosis (Mtb) upon re-entry into other macrophages.Design: Transmission electron microscopy was used to monitor fusion of bacterial phagosomes with late endosomal/lysosomal compartments using thoria as a fluid phase marker. Two-dimensional polyacrylamide gel electrophoresis was used to study bacterial protein expression within macrophages.Results: H37Rv and BCG expressed novel proteins within macrophages. H37Rv also underwent less fusion after intracellular (IC) (24.2±7.7%) than extracellular (XC) (67.4±5.5%) passage when the bacteria entered new macrophages in small clusters. These effects were inhibited by serum, and were not observed with H37Ra or BCG bacteria (78.9±1.6% fused for all conditions). In addition, vacuoles which contained single bacilli were less likely to acquire markers (26.9±2.6%) than those that contained multiple bacilli (77.3±2.8%).Conclusion: These results indicate that phagolysosomal fusion patterns can be modulated by a variety of factors and that virulent Mtb bacteria may express proteins within macrophages that alter their interaction with these host cells.</div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"80 6","pages":"Pages 259-271"},"PeriodicalIF":0.0,"publicationDate":"2000-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.2000.0268","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89596231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Pulmonary surfactant in innate immunity and the pathogenesis of tuberculosis 肺表面活性物质在先天免疫和肺结核发病中的作用

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-08-01 DOI: 10.1054/tuld.2000.0242

J.S. Ferguson , L.S. Schlesinger

引用次数: 45

An esat6 knockout mutant of Mycobacterium bovis produced by homologous recombination will contribute to the development of a live tuberculosis vaccine 通过同源重组产生的牛分枝杆菌esat6基因敲除突变体将有助于结核病活疫苗的开发

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-08-01 DOI: 10.1054/tuld.2000.0244

B.J. Wards , G.W. de Lisle, D.M. Collins

{"title":"An esat6 knockout mutant of Mycobacterium bovis produced by homologous recombination will contribute to the development of a live tuberculosis vaccine","authors":"B.J. Wards , G.W. de Lisle, D.M. Collins","doi":"10.1054/tuld.2000.0244","DOIUrl":"10.1054/tuld.2000.0244","url":null,"abstract":"<div>Setting: Strains of the Mycobacterium tuberculosis complex are being rationally attenuated in order to develop better tuberculosis vaccines than BCG, and it would be helpful if new vaccines lacked an immunogenic protein which could be used as a skin test reagent for determining infection status.Objective: To delete theesat6 gene from a virulent Mycobacterium bovis strain and determine (i) whether this mutant sensitizes guinea pigs to a skin test based on ESAT6 and (ii) what effect this has on the virulence of M. bovis.Design: An homologous recombination technique was used to produce an esat6 knockout mutant of a virulent strain of M. bovis. Guinea pigs were inoculated with either the mutant or parent strain and their reactivity in intradermal skin tests was determined to bovine purified protein derivative (PPD) and recombinant ESAT6 protein.Results: Production of an esat6 knockout strain was demonstrated by Southern blot hybridization and the polymerase chain reaction. Guinea pigs inoculated with either the esat6 knockout strain or its virulent parent had positive skin test reactions to PPD but only animal inoculated with the parent strain had positive skin test reactions to ESAT6. Gross pathology, histopathology and mycobacterial culture of tissues indicated that the knockout strain was less virulent than its parent.Conclusion: If an effective live tuberculosis vaccine can be produced by inactivation of virulence genes in M. bovis, then prior or subsequent knockout of the esat6 gene could contribute to the loss of virulence and enable the development of a test to distinguish between vaccinated and infected animals.</div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"80 4","pages":"Pages 185-189"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.2000.0244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21881128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 124

Influence of relative humidity on particle size and UV sensitivity of Serratia marcescens and Mycobacterium bovis BCG aerosols 相对湿度对粘质沙雷氏菌和牛分枝杆菌卡介苗气溶胶粒径和紫外线敏感性的影响

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-08-01 DOI: 10.1054/tuld.2000.0249

G. Ko, M.W. First, H.A. Burge

{"title":"Influence of relative humidity on particle size and UV sensitivity of Serratia marcescens and Mycobacterium bovis BCG aerosols","authors":"G. Ko, M.W. First, H.A. Burge","doi":"10.1054/tuld.2000.0249","DOIUrl":"10.1054/tuld.2000.0249","url":null,"abstract":"<div>Setting: A study of Serratia marcescens and BCG aerosols.Objective: To evaluate the effect of relative humidity (RH) on (1) the particle size and (2) sensitivity of 254nm germicidal ultraviolet (UV) irradiation.Methods: We built a RH controlled experimental chamber into which bacteria were aerosolized, exposed to varying amounts of UV irradiance over measured time periods, and quantitatively evaluated for viability. Aerosolized Serratia marcescens and bacille Calmette-Guérin (BCG) were subject to UV doses ranging from 57–829 μW · sec/cm2, and sampled with a six-stage Andersen culture plate impactor at RHs ranging from 25–95%.Results: Percent survival for both organisms was inversely related to UV dose. Serratia marcescens was more susceptible to UV than BCG under all conditions. More than 95% of the bacterial aerosol particles were 1.1–4.7 μm in aerodynamic diameter, and particles sizes increased from low (25–36%) to high (85–95%) RH. The count median diameter ranged from 1.9–2.6 μm for Serratia marcescens and from 2.2–2.7 μm for BCG as RH increased. For both Serratia marcescens and BCG, resistance to UV increased as RH increased. The UV resistance of both Serratia marcescens and BCG aerosols dramatically increased at RH higher than 85%.Conclusions: Our results indicate that differences in UV dose, kinds of microorganisms, airborne particle size and RH affect UV susceptibility.</div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"80 4","pages":"Pages 217-228"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.2000.0249","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21880372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 133

The development of wildlife control strategies for eradication of tuberculosis in cattle in Ireland 制定野生动物控制战略以根除爱尔兰牛的结核病

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-08-01 DOI: 10.1054/tuld.2000.0250

E. Gormley , J.D. Collins

{"title":"The development of wildlife control strategies for eradication of tuberculosis in cattle in Ireland","authors":"E. Gormley , J.D. Collins","doi":"10.1054/tuld.2000.0250","DOIUrl":"10.1054/tuld.2000.0250","url":null,"abstract":"<div>Wildlife species, such as badgers, act as maintenance hosts for Mycobacterium bovis and contribute to the spread and persistence of tuberculosis in associated cattle populations. In areas in which there is a tuberculosis problem affecting a number of herds, the involvement of infected wildlife in the introduction of M. bovis infection into herds act as a constraint to eradication of the disease. Epidemiological evidence demonstrates a high prevalence of tuberculosis in badgers, and controlled studies involving comprehensive badger removal have shown that this strategy can serve to significantly reduce cattle reactor rates in the targeted areas. However, as the badger is a protected wildlife species, alternative strategies are required to combat the disease. Targeted vaccination of wildlife species against tuberculosis is an option which, if successfully employed, could directly facilitate the advancement of bovine tuberculosis eradication in affected areas. Any proposed vaccination programme would need to be undertaken against the background of an exhaustive investigation of the cattle and herd management-related factors, and take account of environmental issues.</div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"80 4","pages":"Pages 229-236"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.2000.0250","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21880373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 62

Deletion of the putative antioxidant noxR1 does not alter the virulence of Mycobacterium tuberculosis H37Rv 删除假定的抗氧化noxR1不会改变结核分枝杆菌H37Rv的毒力

Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease Pub Date : 2000-08-01 DOI: 10.1054/tuld.2000.0251

G.R. Stewart , S. Ehrt , L.W. Riley , J.W. Dale , J. McFadden

{"title":"Deletion of the putative antioxidant noxR1 does not alter the virulence of Mycobacterium tuberculosis H37Rv","authors":"G.R. Stewart , S. Ehrt , L.W. Riley , J.W. Dale , J. McFadden","doi":"10.1054/tuld.2000.0251","DOIUrl":"10.1054/tuld.2000.0251","url":null,"abstract":"<div>Setting: The cloned M. tuberculosis noxR1 gene has been shown to confer resistance to reactive nitrogen intermediates (RNI) and reactive oxygen intermediates (ROI) upon Escherichia coli and Mycobacterium smegmatis.Objective: To investigate the role of noxR1 in resistance to RNI and virulence of M. tuberculosis.Design: The noxR1 gene was deleted from M. bovis BCG and M. tuberculosis H37Rv by allelic exchange. The mutants were compared to wild type strains with respect to resistance to chemically generated RNI. The virulence of the M. tuberculosis mutant was investigated in a murine model of infection.Results: The NoxR1 mutants grew normally in Sautons and 7H9 broths. The BCG mutant demonstrated decreased resistance to in vitro generated RNI compared to the wild type. Resistance to RNI could be restored to the mutant by reintroduction of the noxR1 locus on a replicating plasmid. However, deletion of noxR1 from M. tuberculosis H37Rv did not result in decreased resistance to RNI nor a difference in growth and survival of the bacterium during murine infection.Conclusion: The noxR1 gene locus in M. bovis BCG bestows ability to resist RNI generated in vitro. In M. tuberculosis H37Rv, however, noxR1 is either not involved in RNI resistance and virulence or is better compensated for by other mechanisms.</div>","PeriodicalId":77450,"journal":{"name":"Tubercle and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease","volume":"80 4","pages":"Pages 237-242"},"PeriodicalIF":0.0,"publicationDate":"2000-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1054/tuld.2000.0251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21880374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12