相对湿度对粘质沙雷氏菌和牛分枝杆菌卡介苗气溶胶粒径和紫外线敏感性的影响

G. Ko, M.W. First, H.A. Burge
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引用次数: 133

摘要

环境:粘质沙雷菌和卡介苗气雾剂的研究。目的:评价相对湿度(RH)对254nm杀菌紫外线(UV)辐照(1)粒径和(2)灵敏度的影响。方法:我们建立了一个RH控制的实验室,将细菌雾化,在测量的时间段内暴露在不同量的紫外线照射下,并定量评估其生存能力。将粘质沙雷氏菌和卡介苗(bacillus calmetet - gusamrin, BCG)雾化于57 ~ 829 μW·sec/cm2的紫外线照射下,用六段Andersen培养板冲击器在25 ~ 95%的RHs范围内取样。结果:两种生物的存活率与紫外线剂量呈负相关。粘质沙雷菌对紫外光的敏感性高于卡介苗。95%以上的细菌气溶胶颗粒的空气动力直径在1.1 ~ 4.7 μm之间,粒径由低RH(25 ~ 36%)逐渐增大到高RH(85 ~ 95%)。随着RH的升高,粘质沙雷氏菌的中位直径为1.9 ~ 2.6 μm,卡介苗的中位直径为2.2 ~ 2.7 μm。粘质沙雷菌和卡介苗对紫外线的抗性随RH的增加而增加。当相对湿度大于85%时,粘质沙雷菌和卡介苗气溶胶的抗紫外线能力均显著增强。结论:紫外线照射剂量、微生物种类、空气中颗粒物大小、相对湿度等因素对紫外光敏感性有影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Influence of relative humidity on particle size and UV sensitivity of Serratia marcescens and Mycobacterium bovis BCG aerosols

Setting: A study of Serratia marcescens and BCG aerosols.

Objective: To evaluate the effect of relative humidity (RH) on (1) the particle size and (2) sensitivity of 254nm germicidal ultraviolet (UV) irradiation.

Methods: We built a RH controlled experimental chamber into which bacteria were aerosolized, exposed to varying amounts of UV irradiance over measured time periods, and quantitatively evaluated for viability. Aerosolized Serratia marcescens and bacille Calmette-Guérin (BCG) were subject to UV doses ranging from 57–829 μW · sec/cm2, and sampled with a six-stage Andersen culture plate impactor at RHs ranging from 25–95%.

Results: Percent survival for both organisms was inversely related to UV dose. Serratia marcescens was more susceptible to UV than BCG under all conditions. More than 95% of the bacterial aerosol particles were 1.1–4.7 μm in aerodynamic diameter, and particles sizes increased from low (25–36%) to high (85–95%) RH. The count median diameter ranged from 1.9–2.6 μm for Serratia marcescens and from 2.2–2.7 μm for BCG as RH increased. For both Serratia marcescens and BCG, resistance to UV increased as RH increased. The UV resistance of both Serratia marcescens and BCG aerosols dramatically increased at RH higher than 85%.

Conclusions: Our results indicate that differences in UV dose, kinds of microorganisms, airborne particle size and RH affect UV susceptibility.

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