Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)最新文献_第7页

Chemical and thermal inhibition of protein secretion have stage specific effects on the intraerythrocytic development of Plasmodium falciparum in vitro. 化学和热抑制蛋白分泌对体外恶性疟原虫红细胞内发育具有阶段性特异性作用。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

J Benting, I Ansorge, K Paprotka, K R Lingelbach

{"title":"Chemical and thermal inhibition of protein secretion have stage specific effects on the intraerythrocytic development of Plasmodium falciparum in vitro.","authors":"J Benting, I Ansorge, K Paprotka, K R Lingelbach","doi":"","DOIUrl":"","url":null,"abstract":"The intraerythrocytic stages of the human malaria parasite Plasmodium falciparum induce a variety of physiological changes of the host erythrocyte. Many proteins are secreted from the parasite and are subsequently found at specific locations within the host cell. To elucidate the importance of protein secretion for parasite survival, infected red blood cells (IRBC) were subjected to the fungal metabolite brefeldin A (BFA) and to incubation at 15 degrees C, treatments that inhibit protein secretion and parasite development. Evidence is provided that retardation of parasite development in the presence of BFA correlates with an inhibition of protein secretion. Incubation at 15 degrees C and BFA reversibly arrest parasite development at the ring stage. Arrested ring stages loose 50% of their competence to develop to trophozoites after 1.5 days of treatment with BFA and after approximately 4 days at 15 degrees C. BFA affects development of trophozoites at concentrations similar to those required to arrest rings. In contrast to rings, the viability of trophozoites cultured at 15 degrees C or in the presence of BFA is completely abolished within 24 h.","PeriodicalId":77449,"journal":{"name":"Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)","volume":"45 4","pages":"303-7"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18718272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parasitological and clinical studies on Wuchereria bancrofti infection in Chuuk (formerly Truk) State, Federated States of Micronesia. 密克罗尼西亚联邦楚克(原楚克)州班氏乌切利氏菌感染的寄生虫学和临床研究。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

E Kimura, K Remit, M Fujiwara, K Aniol, N Siren

引用次数: 0

Development and evaluation of an enzyme linked immunotransfer blot technique for serodiagnosis of Chagas' disease. 酶联免疫转移印迹血清诊断南美锥虫病技术的建立与评价。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

M G Teixeira, J Borges-Pereira, E Netizert, M L Souza, J M Peralta

引用次数: 0

Onchocerca volvulus: in vitro cytotoxic effects of human neutrophils and serum on third-stage larvae. 盘尾盘尾虫:人中性粒细胞和血清对第三期幼虫的体外细胞毒作用。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

E H Johnson, M Irvine, P H Kass, J Browne, M Abdullai, A M Prince, S Lustigman

{"title":"Onchocerca volvulus: in vitro cytotoxic effects of human neutrophils and serum on third-stage larvae.","authors":"E H Johnson, M Irvine, P H Kass, J Browne, M Abdullai, A M Prince, S Lustigman","doi":"","DOIUrl":"","url":null,"abstract":"The cytotoxic effects of neutrophils co-cultured with infective third-stage larvae (L3) and autologous serum from three groups of individuals: infected (INF), non-patent endemic normals (EN) and non-endemic controls (NEC), were compared using a MTT (3-(4,5 dimethylthiazol-2yl)-2,5 diphenyl tetrazolium bromide) reduction assay as a biochemical parameter of larval viability. The MTT assay permitted the detection of L3 which had suffered varying levels of necrosis. The observations from this study indicate that infected individuals and endemic normals are capable of mounting a specific immune response against the infective L3. This is based on our findings of i) significantly greater numbers of L3 showed necrosis in neutrophil cultures of the INF and EN compared to those from the NEC, ii) a superior ability of cultures from these individuals to inhibit the molting of L3 to fourth-stage larvae, iii) IgG antibodies reactive with the surface of L3, detected by immunofluorescence in the serum of the INF and the EN and iv) recognition of specific antigens in extracts of L3 detected by Western blot analysis. The specific immune response directed against L3 is probably a significant immunological defense mechanism used to limit infection in endemic areas.","PeriodicalId":77449,"journal":{"name":"Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)","volume":"45 4","pages":"331-5"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18718278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complement sensitivity of Entamoeba histolytica and various nonpathogenic amoeba species. 溶组织内阿米巴和各种非致病性阿米巴的补体敏感性。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

B Förster, F Ebert, R D Horstmann

引用次数: 0

Knowledge and attitudes toward onchocerciasis in the Thyolo highlands of Malawi. 马拉维Thyolo高地对盘尾丝虫病的认识和态度。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

K Johnston, P Courtright, G Burnham

引用次数: 0

Stage specific differences in steady state levels of mRNA encoding the major surface glycoprotein of Brugia pahangi. pahangi主要表面糖蛋白编码mRNA稳态水平的分期特异性差异。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

J Cox-Singh, M J Paine, S A Martin, E Devaney

引用次数: 0

Mixed populations of Trypanosoma brucei in wild Glossina palpalis palpalis. 野生舌蝇中布鲁氏锥虫的混合种群。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

J R Stevens, F Mathieu-Daudé, J J McNamara, V H Mizen, A Nzila

{"title":"Mixed populations of Trypanosoma brucei in wild Glossina palpalis palpalis.","authors":"J R Stevens, F Mathieu-Daudé, J J McNamara, V H Mizen, A Nzila","doi":"","DOIUrl":"","url":null,"abstract":"In many previous characterization studies of Trypanozoon, isolates have been subpassaged numerous times in laboratory rodents until a quantity of trypanosomes sufficient for analysis has been obtained. In addition to the numerous biochemical effects of such a process on the parasite, it appears probable that adaptation to an unnatural host may also serve to filter out less virulent populations from mixed infections, leading to an underestimate of the true level of genetic diversity. By the early cloning of trypanosomes from susceptible captive flies infected from the primary isolate--the midgut of a wild tsetse--the present study provides evidence of the range of genetically different Trypanosoma brucei populations which may coexist within the midgut of individual tsetse flies in nature. The three primary isolates from tsetse yielded one, five and nine genetically distinct populations. Cloned populations were confirmed as T. brucei using the polymerase chain reaction, and were characterized by karyotype analysis and multilocus isoenzyme electrophoresis. These data allowed a limited assessment of the level of genetic variability in natural populations of T. brucei.","PeriodicalId":77449,"journal":{"name":"Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)","volume":"45 4","pages":"313-8"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18718274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urinary and intestinal schistosomiasis in the Tono Irrigation Scheme, Kassena/Nankana District, upper east region, Ghana. 加纳上东部地区卡塞纳/南卡纳区托诺灌溉计划的泌尿和肠道血吸虫病。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

J A Amankwa, P Bloch, J Meyer-Lassen, A Olsen, N O Christensen

{"title":"Urinary and intestinal schistosomiasis in the Tono Irrigation Scheme, Kassena/Nankana District, upper east region, Ghana.","authors":"J A Amankwa, P Bloch, J Meyer-Lassen, A Olsen, N O Christensen","doi":"","DOIUrl":"","url":null,"abstract":"Human- and snail-related aspects of transmission of urinary and intestinal schistosomiasis were studied in the Tono Irrigation Scheme in northern Ghana. The scheme became operational in 1977. In some schools, prevalences and intensities of both Schistosoma mansoni and S. haematobium infection were alarmingly high, pointing to human schistosomiasis being at least focally a health problem of major public health concern. Positive correlations between intensity of schistosome infection, as measured by egg output, with frequency of visible haematuria and history of haematuria point to opportunities for community-based assessment of morbidity and identification of high risk population subgroups. Bulinus globosus is the most important snail host for S. haematobium while Biomphalaria pfeifferi serves as host for S. mansoni. While transmission of S. mansoni is taking place only in the main canal, transmission of S. haematobium takes place in all parts of the irrigation system (lateral canal, night storage dam, main reservoir). Transmission of both S. mansoni and S. haematobium is rather focal and a seasonal pattern of transmission is indicated with peak transmission taking place during the beginning of the dry season. The high endemicity and the transmission patterns described in this study call for an integrated approach to schistosomiasis morbidity control in the area.","PeriodicalId":77449,"journal":{"name":"Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)","volume":"45 4","pages":"319-23"},"PeriodicalIF":0.0,"publicationDate":"1994-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18718275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human infection with Gongylonema pulchrum: a case report. 人感染弓形线虫1例。

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

T Jelinek, T Löscher

引用次数: 0