Development and evaluation of an enzyme linked immunotransfer blot technique for serodiagnosis of Chagas' disease.

Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ) Pub Date : 1994-12-01

M G Teixeira, J Borges-Pereira, E Netizert, M L Souza, J M Peralta

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Abstract

An enzyme linked immunoelectrotransfer blot technique (EITB) was developed and evaluated for the serodiagnosis of Chagas' disease. EITB strips were prepared using Trypansoma cruzi (Y strain) epimastigotes lysate. Evaluation was performed with 235 serum samples collected from individuals living in an endemic area for Chagas' disease. Among those samples, 160 were serological positive in three conventional tests for T. cruzi and 75 were negative. The specificity was determined using 37 serum samples from patients with other infectious diseases. The EITB test showed sensitivity of 99.3% and specificity of 100% when a positive reaction was defined as the presence of 3 bands from a group of 7 (14, 19, 27, 30, 34, 37 and 75), a negative reaction was defined as the absence of 6 of the 7 bands, and an indeterminate reaction as the presence of two of the 7 bands.

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酶联免疫转移印迹血清诊断南美锥虫病技术的建立与评价。

建立了一种酶联免疫电转移印迹技术(EITB)，并对其用于南美锥虫病的血清诊断进行了评价。采用克氏锥虫(Y株)附毛鞭毛虫裂解液制备EITB试纸条。对从生活在南美锥虫病流行地区的个人收集的235份血清样本进行了评估。在这些样本中，有160份在三项常规克氏体检测中呈血清学阳性，75份呈阴性。使用其他传染病患者的37份血清样本确定特异性。当7个条带(14、19、27、30、34、37和75)中有3个条带存在为阳性反应，7个条带中有6个条带不存在为阴性反应，7个条带中有2个条带存在为不确定反应时，EITB检测的灵敏度为99.3%，特异性为100%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit (GTZ)

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