Second messengers and phosphoproteins最新文献_第5页

Molecular cloning and sequence analysis of two distinct types of Xenopus laevis protein kinase C. 两种非洲爪蟾蛋白激酶C的分子克隆及序列分析。

Second messengers and phosphoproteins Pub Date : 1988-01-01

K H Chen, Z G Peng, S Lavu, H F Kung

引用次数: 0

Effect of thromboxane antagonists on prostaglandin regulation of platelet adenylate cyclase. 血栓素拮抗剂对前列腺素调控血小板腺苷酸环化酶的影响。

Second messengers and phosphoproteins Pub Date : 1988-01-01

B Ashby

引用次数: 0

Forskolin binding to intact S49 lymphoma cells. Forskolin与完整S49淋巴瘤细胞的结合。

Second messengers and phosphoproteins Pub Date : 1988-01-01

R Barber

引用次数: 0

Modification of adenylate cyclase by photoaffinity analogs of forskolin. 福斯克林光亲和类似物修饰腺苷酸环化酶。

Second messengers and phosphoproteins Pub Date : 1988-01-01

L T Ho, Z M Nie, T J Mende, S Richardson, A Chavan, E Kolaczkowska, D S Watt, B E Haley, R J Ho

{"title":"Modification of adenylate cyclase by photoaffinity analogs of forskolin.","authors":"L T Ho, Z M Nie, T J Mende, S Richardson, A Chavan, E Kolaczkowska, D S Watt, B E Haley, R J Ho","doi":"","DOIUrl":"","url":null,"abstract":"Photoaffinity labeling analogs of the adenylate cyclase activator forskolin (PF) have been synthesized, purified and tested for their effect on preparations of membrane-bound, Lubrol solubilized and forskolin affinity-purified adenylate cyclase (AC). All analogs of forskolin significantly activated AC. However, in the presence of 0.1 to 0.3 microM forskolin, the less active forskolin photoaffinity probes at 100 microM caused inhibition. This inhibition was dose-dependent for PF, suggesting that PF may complete with F for the same binding site(s). After cross-linking [125I]PF-M (see Figure 1 for structure) to either membrane or Lubrol-solubilized AC preparations by photolysis, a radiolabeled 100-110 kDa protein band was observed after autoradiography following SDS-PAGE. F at 100 microM blocked the photoradiolabeling of this protein. Radioiodination of forskolin-affinity purified AC showed several protein bands on autoradiogram, however, only one band (Mr = 100-110 kDa) was specifically labeled by [125I]PF-M following photolysis. The photoaffinity-labeled protein of 100-110 kDa of AC preparation of rat adipocyte may be the catalytic unit of adenylate cyclase of rat adipocyte itself as supported by the facts that [a] no other AC-regulatory proteins are known to be of this size, [b] the catalytic unit of bovine brain enzyme is in the same range and [c] this PF specifically stimulates AC activity when assayed alone, and weekly inhibits forskolin-activation of cyclase. These studies indicate that radiolabeled PF probes may be useful for photolabeling and detecting the catalytic unit of adenylate cyclase.","PeriodicalId":77384,"journal":{"name":"Second messengers and phosphoproteins","volume":"12 5-6","pages":"209-23"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14401825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The adenosine Ri agonist, phenylisopropyladenosine, reduces high affinity isoproterenol binding to the beta-adrenergic receptor of rat myocardial membranes. 腺苷Ri激动剂，苯异丙基腺苷，减少高亲和力异丙肾上腺素与大鼠心肌膜β -肾上腺素能受体的结合。

Second messengers and phosphoproteins Pub Date : 1988-01-01

F D Romano, R A Fenton, J G Dobson

{"title":"The adenosine Ri agonist, phenylisopropyladenosine, reduces high affinity isoproterenol binding to the beta-adrenergic receptor of rat myocardial membranes.","authors":"F D Romano, R A Fenton, J G Dobson","doi":"","DOIUrl":"","url":null,"abstract":"Adenosine attenuates beta-adrenergic receptor mediated activation of adenylate cyclase in myocardial membranes via adenosine Ri receptors. The effects of adenosine analogs on the binding characteristics of beta-adrenergic receptors were examined in the present study utilizing rat ventricular membranes treated with adenosine deaminase. In 125I-cyanopindolol/isoproterenol competitive binding experiments phenylisopropyladenosine (PIA) significantly increased the IC50 for isoproterenol from 48 +/- 6 nM to 140 +/- 48 nM and steepened the slope of the competition curves from -0.56 +/- 0.03 to -0.90 +/- 0.21. Computer analysis of these curves indicated that binding of isoproterenol to the high affinity state of the beta-adrenergic receptor was eliminated in the presence of PIA. PIA had no effects in the presence of GPP(NH)P. 2-chloroadenosine, a less specific Ri agonist, caused smaller increases in IC50 and slope, without significantly affecting high affinity binding. 2',5'-dideoxyadenosine, a P-site agonist, had no significant effects on isoproterenol binding. During the time course of the competitive binding experiments the membranes displayed isoproterenol-sensitive adenylate cyclase activity in the absence of added GTP. These data suggest that adenosine attenuates catecholamine-induced activation of adenylate cyclase via Ri receptors by decreasing the ability of beta-adrenergic agonists to promote the formation of a high affinity complex composed of the agonist, receptor and stimulatory guanine nucleotide binding protein.","PeriodicalId":77384,"journal":{"name":"Second messengers and phosphoproteins","volume":"12 1","pages":"29-43"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13986219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resolution of soluble rat cardiac phosphodiesterases by high performance liquid chromatography. 高效液相色谱法分离可溶性大鼠心脏磷酸二酯酶。

Second messengers and phosphoproteins Pub Date : 1988-01-01

D C Bode, J R Kanter, L L Brunton

引用次数: 0

Somatostatin stimulates phosphodiesterase in rat anterior pituitary and brain, and GH4C1 cells. 生长抑素刺激大鼠垂体前叶和脑内磷酸二酯酶及GH4C1细胞。

Second messengers and phosphoproteins Pub Date : 1988-01-01

M C Rendón, M J Toro, M Mellado, E Montoya

引用次数: 0

Protein kinase C activity in normal and neoplastic squamous epithelia from the upper aero-digestive tract. 蛋白激酶C在上消化道正常和肿瘤鳞状上皮中的活性。

Second messengers and phosphoproteins Pub Date : 1988-01-01

E L Rydell, K L Axelsson, J Olofsson

{"title":"Protein kinase C activity in normal and neoplastic squamous epithelia from the upper aero-digestive tract.","authors":"E L Rydell, K L Axelsson, J Olofsson","doi":"","DOIUrl":"","url":null,"abstract":"In the present study the protein kinase activity was determined in surgical specimens of squamous cell carcinoma from the upper aero-digestive tract, and the activity was compared with the activity in normal mucosa obtained from the same location in patients undergoing surgery for non-neoplastic diseases. The basal protein kinase activity in the soluble fraction was about 15-fold higher in tumors as compared to in normal mucosa. The difference between protein kinase activity in the presence of Ca2+ and presence/absence of phosphatidylserine/diolein was taken as the activity of protein kinase C. Protein kinase C activity in the presence of 0.5 mM Ca2+ was 10.0 +/- 2.1 pmol 32P/mg prot. x min in tumors (n = 19) and 2.4 +/- 0.7 pmol 32P/mg prot. x min. in normal mucosa (n = 6). A similar difference was obtained with 1 mM Ca2+. An even greater difference in protein kinase C activity was seen when the soluble enzyme had been partially purified by ion-exchange chromatography on DE-52 columns. In this case a 7-fold higher protein kinase activity was found in tumors as compared to in normal mucosa. In the particulate fraction the basal protein kinase activity was about 3-fold higher in tumors as compared to in normal mucosa. Protein kinase C activity in the particulate fraction, defined as described above, was 10.7 +/- 4.1 pmol 32P/mg prot. x min in tumors and 6.1 +/- 4.4 pmol 32P/mg prot. x min in normal mucosa. These results show a significantly higher activity in both total protein kinase activity and protein kinase C activity in epithelial tumors from the upper aero-digestive tract.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77384,"journal":{"name":"Second messengers and phosphoproteins","volume":"12 4","pages":"155-62"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14375168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Types I alpha and I beta isozymes of cGMP-dependent protein kinase: alternative mRNA splicing may produce different inhibitory domains. cgmp依赖性蛋白激酶I型α和I型β同工酶:不同的mRNA剪接可能产生不同的抑制结构域。

Second messengers and phosphoproteins Pub Date : 1988-01-01

S H Francis, T A Woodford, L Wolfe, J D Corbin

{"title":"Types I alpha and I beta isozymes of cGMP-dependent protein kinase: alternative mRNA splicing may produce different inhibitory domains.","authors":"S H Francis, T A Woodford, L Wolfe, J D Corbin","doi":"","DOIUrl":"","url":null,"abstract":"We recently described a novel isozyme of cGMP-dependent protein kinase (type I beta). It has a structure and peptide substrate specificity which is similar to that of type I alpha, but it has a different cGMP binding behavior, and autophosphorylation occurs almost entirely in serine instead of in both serine and threonine residues (Wolfe, L., Corbin, J.D., and Francis, S.H. (1989) J. Biol. Chem. 264, 7734-7741). An amino-terminal sequence of 31 amino acids derived from three proteolytic fragments of type I beta had 45% homology with a sequence beginning at type I alpha-47. However, sequences of three CNBr peptides of type I beta were identical to sequences of type I alpha beginning at type I alpha-202, -213, and -576 of 11, 27, and 30 residues. These sequences include portions of the catalytic domain and at least one cGMP-binding domain (site 1). Thus, types I alpha and I beta may be produced by alternative splicing of two unique mRNA segments to generate different amino acid sequences in the protein in a region that is amino-terminal to type I alpha-202. This segment in type I beta corresponds to the region in type I alpha that includes the major autophosphorylation site (Thr-58) which is within the domain that is proposed to inhibit catalytic activity. This region presumably interacts with the cGMP-binding site(s) to account for the differences in cGMP-binding behavior between types I alpha and I beta. Even though the sequence of type I beta in the variable region lacks the residue corresponding to Thr-58, it includes a consensus phosphorylation site (KRQAISA) beginning at type I alpha-59, which is absent in type I alpha. The results imply flexibility in the design of the autophosphorylation site and, hence, of the inhibitory domain.","PeriodicalId":77384,"journal":{"name":"Second messengers and phosphoproteins","volume":"12 5-6","pages":"301-10"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14401824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The stimulation of a casein kinase II from yeast by polyamines occurs with endogenous substrates at cytosolic salt levels. 多胺对酵母酪蛋白激酶II的刺激发生在胞质盐水平的内源性底物上。

Second messengers and phosphoproteins Pub Date : 1988-01-01

M Nuutinen, J Londesborough

引用次数: 0