{"title":"高效液相色谱法分离可溶性大鼠心脏磷酸二酯酶。","authors":"D C Bode, J R Kanter, L L Brunton","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A high performance liquid chromatographic method has been developed to separate isozymes of cyclic nucleotide phosphodiesterase. The method employs a polymer-based anion exchange column eluted with a sodium chloride gradient. Compared to traditional chromatography over DEAE-cellulose, the method is more rapid (30 min), dilutes the sample less, achieves better resolution of kinetically distinct forms, may be applied to as little as 200 micrograms of tissue protein and is appropriate for analytical use.</p>","PeriodicalId":77384,"journal":{"name":"Second messengers and phosphoproteins","volume":"12 5-6","pages":"235-40"},"PeriodicalIF":0.0000,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Resolution of soluble rat cardiac phosphodiesterases by high performance liquid chromatography.\",\"authors\":\"D C Bode, J R Kanter, L L Brunton\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A high performance liquid chromatographic method has been developed to separate isozymes of cyclic nucleotide phosphodiesterase. The method employs a polymer-based anion exchange column eluted with a sodium chloride gradient. Compared to traditional chromatography over DEAE-cellulose, the method is more rapid (30 min), dilutes the sample less, achieves better resolution of kinetically distinct forms, may be applied to as little as 200 micrograms of tissue protein and is appropriate for analytical use.</p>\",\"PeriodicalId\":77384,\"journal\":{\"name\":\"Second messengers and phosphoproteins\",\"volume\":\"12 5-6\",\"pages\":\"235-40\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1988-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Second messengers and phosphoproteins\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Second messengers and phosphoproteins","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Resolution of soluble rat cardiac phosphodiesterases by high performance liquid chromatography.
A high performance liquid chromatographic method has been developed to separate isozymes of cyclic nucleotide phosphodiesterase. The method employs a polymer-based anion exchange column eluted with a sodium chloride gradient. Compared to traditional chromatography over DEAE-cellulose, the method is more rapid (30 min), dilutes the sample less, achieves better resolution of kinetically distinct forms, may be applied to as little as 200 micrograms of tissue protein and is appropriate for analytical use.
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