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SAAS bulletin, biochemistry and biotechnology最新文献

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Poliovirus RNA recombination. 脊髓灰质炎病毒RNA重组。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1991-01-01
J Pata, K Kirkegaard
{"title":"Poliovirus RNA recombination.","authors":"J Pata,&nbsp;K Kirkegaard","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We are developing an in vitro system for poliovirus RNA recombination. In this system, two mutant RNAs are replicated with poliovirus RNA-dependent RNA polymerase. Recombination will produce RNAs containing neither mutation and will be the only progeny RNAs that are infectious. We will use this system to determine what proteins and reaction conditions are required for recombination and to study the details of the mechanism of recombination.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"4 ","pages":"20-1"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of cytokines on bovine mammary gland immunity. 细胞因子对牛乳腺免疫的影响。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1991-01-01
S C Nickerson
{"title":"Effect of cytokines on bovine mammary gland immunity.","authors":"S C Nickerson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cytokines are a family of glycoproteins produced by various cell types in response to specific stimuli that regulate the immune response. This paper reviews recent studies on two different cytokines, each with its own effector cell type: interleukin-2 (IL-2), which regulates lymphoid cell responses; and granulocyte colony-stimulating factor (GCSF), which regulates neutrophil responses. In the first study, administration of IL-2 to the bovine mammary gland was found to stimulate the expansion of lymphocyte populations and increase local antibody production. In the second study, systemic administration of GCSF increased peripheral blood as well as milk neutrophil populations, which afforded some protection against Staphylococcus aureus challenge. Results suggest a role for cytokines in the control of mastitis in dairy cattle.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"4 ","pages":"60-7"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of sites of pre-MRNA/spliceosome association. 前mrna /剪接体结合位点的鉴定。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1991-01-01
B C Rymond
{"title":"Identification of sites of pre-MRNA/spliceosome association.","authors":"B C Rymond","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>RNase H and synthetic DNA oligonucleotides were used to analyze the ribonucleoprotein (RNP) structure of the yeast spliceosome and to assay the pre-mRNA sequence requirements for step 1 of splicing. The data suggest that tight, stable contacts between the pre-mRNA and the spliceosome may be limited to the 5' splice site and branch point regions of the intron. A 30 nucleotide segment 3' of the branch point was found to be necessary for spliceosome maturation and essential for step 1 of splicing. Somewhat surprisingly, the 3' splice site was sensitive to nuclease digestion and completely dispensable for step 1 of splicing.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"4 ","pages":"76-80"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A molecular model for illegitimate recombination in Bacillus subtilis. 枯草芽孢杆菌非法重组的分子模型。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1991-01-01
K B Temeyer, K M Hopkins, L F Chapman
{"title":"A molecular model for illegitimate recombination in Bacillus subtilis.","authors":"K B Temeyer,&nbsp;K M Hopkins,&nbsp;L F Chapman","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The recombinant DNA junctions at which pUB110 and Bacillus subtilis chromosomal DNA were joined to form the plasmid pKBT1 were cloned and sequenced. From the sequencing data we conclude that the pUB110 sequence is intact in the pair of cloned pKBT1 fragments and pTL12 sequences are not present. A molecular model for the formation of pKBT1 based on structural motifs characteristic of the joint sites is presented.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"4 ","pages":"52-5"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Eukaryotic gene regulation: simple vs complex models. 真核生物基因调控:简单vs复杂模型。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1991-01-01
J C Swaffield, S A Johnston
{"title":"Eukaryotic gene regulation: simple vs complex models.","authors":"J C Swaffield,&nbsp;S A Johnston","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The current generally accepted model of eukaryotic gene regulation is essentially a simple one. Regulatory proteins containing separable DNA binding and transcriptional activation domains, bind to specific DNA sequences in promotors and interact directly or indirectly with the TATA Box binding factor to increase the rate of transcription initiation at selected promotors. Here we present observations suggesting that the process may be more complex.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"4 ","pages":"17-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12540561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Epitope mapping in Salmonella flagellar protein. 沙门氏菌鞭毛蛋白的表位定位。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1991-01-01
T M Joys
{"title":"Epitope mapping in Salmonella flagellar protein.","authors":"T M Joys","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The flagellar filaments of bacteria of the genus Salmonella are highly immunopotent and antigenically diverse. It is proposed to develop vaccines by replacing the flagella of live attenuated Salmonella strains with engineered flagellar filament proteins containing foreign epitopes of medical and agricultural importance. As an initial step in this process, the major linear epitope regions of one filament protein have been identified.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"4 ","pages":"56-9"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural transformation in Acinetobacter calcoaceticus. 钙酸不动杆菌的自然转化。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1990-01-01
M S Shanley, M Ahmadian-Tehrani, R C Benjamin, H F Leher
{"title":"Natural transformation in Acinetobacter calcoaceticus.","authors":"M S Shanley,&nbsp;M Ahmadian-Tehrani,&nbsp;R C Benjamin,&nbsp;H F Leher","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Acinetobacter calcoaceticus is a metabolically versatile microorganism that is naturally competent for DNA uptake and incorporation. We have exploited the natural state of competency for studies involving the cloning, organization and expression of genes encoding catabolic enzymes. A. calcoaceticus is able to take up, at high efficiency, genetically engineered DNA, incorporate the DNA and stably maintain and express the DNA. Sequence analysis of cloned A. calcoaceticus DNA reveals a great deal of internal repetition and secondary structure, but no specific sequences associated with uptake appear to be present. Uptake and transformation occurs in solid and liquid medium, at a wide range of DNA concentrations and with little restriction barrier to the source of the transforming DNA.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"3 ","pages":"27-31"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cooperative interactions in transcriptional regulation. 转录调控中的合作相互作用。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1990-01-01
M G Fried
{"title":"Cooperative interactions in transcriptional regulation.","authors":"M G Fried","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Cooperative interactions between regulatory proteins and RNA polymerase are a common feature of transcriptional systems. We have developed a method, based on the electrophoresis mobility shift assay, for the measurement of cooperative effects in the binding of proteins to DNA restriction fragments. Using this approach we have identified a hitherto unknown interaction between the E. coli lactose repressor and CAP proteins. We suggest that this interaction plays a role in the control of the lactose operon that is not predicted by current regulatory models.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"3 ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fat-body-specific expression of the Drosophila Lsp-2 gene. 果蝇Lsp-2基因的脂肪体特异性表达。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1990-01-01
H Benes, D W Spivey, J Miles, K Neal, R G Edmondson
{"title":"Fat-body-specific expression of the Drosophila Lsp-2 gene.","authors":"H Benes,&nbsp;D W Spivey,&nbsp;J Miles,&nbsp;K Neal,&nbsp;R G Edmondson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The larval serum protein-2 gene (Lsp-2) of Drosophila melanogaster is expressed at a very high level in the fat body of third-instar larvae. Here we report that Lsp-2 transcription in adult flies produces a unique mRNA localized in the adult adipose tissue of the head in both sexes. To identify regulatory regions of this Drosophila gene, Lsp-2 5'-flanking DNA sequences were fused to the E. coli beta-galactosidase gene (lacZ). Transient expression of the hybrid gene in third-instar larvae indicates that 230 bp just upstream from the 'TATA box' of the Lsp-2 gene are sufficient for larval fat body-specific expression.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"3 ","pages":"129-33"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12542383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Zinc finger structure of a ribosomal gene-specific transcription factor. 核糖体基因特异性转录因子的锌指结构。
SAAS bulletin, biochemistry and biotechnology Pub Date : 1990-01-01
J S Hanas, R M Littell, C J Gaskins, R Zebrowski
{"title":"Zinc finger structure of a ribosomal gene-specific transcription factor.","authors":"J S Hanas,&nbsp;R M Littell,&nbsp;C J Gaskins,&nbsp;R Zebrowski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Xenopus transcription factor IIIA (TFIIIA) or TFIIIA mutants with internal deletions were expressed in E.coli, isolated from E.coli cell extracts, and identified by SDS PAGE and immunoblotting with rabbit antiserum against native TFIIIA. Specific DNA binding of intact or internally deleted TFIIIA was compared by analyzing their abilities to protect the internal control region (ICR) of the Xenopus 5S ribosomal RNA gene from DNase I digestion. Intact protein bound specifically to the entire ICR (+96 to +43). One TFIIIA deletion mutant, expressed from cDNA lacking the coding sequence for the putative fourth zinc finger protected the ICR from DNase I digestion from nucleotide positions +96 to +78. A second TFIIIA mutant resulting from fusion of putative zinc fingers 7 and 8 protected the 5S gene ICR from positions +96 to +63. The regions of the protein comprising the N-terminal 3 fingers and N-terminal six fingers appear to be in contact with approximately 18 and 33 bp of DNA respectively on the 3' side of the 5S gene ICR.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"3 ","pages":"85-90"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12540833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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