枯草芽孢杆菌非法重组的分子模型。

K B Temeyer, K M Hopkins, L F Chapman
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引用次数: 0

摘要

对pUB110与枯草芽孢杆菌染色体DNA连接形成质粒pKBT1的重组DNA连接点进行克隆和测序。从测序数据我们得出结论,pUB110序列在克隆的pKBT1片段中是完整的,pTL12序列不存在。提出了一种基于关节位点结构基序特征的pKBT1形成的分子模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A molecular model for illegitimate recombination in Bacillus subtilis.

The recombinant DNA junctions at which pUB110 and Bacillus subtilis chromosomal DNA were joined to form the plasmid pKBT1 were cloned and sequenced. From the sequencing data we conclude that the pUB110 sequence is intact in the pair of cloned pKBT1 fragments and pTL12 sequences are not present. A molecular model for the formation of pKBT1 based on structural motifs characteristic of the joint sites is presented.

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