{"title":"Molecular cloning of the xylL-xylE region from the P. putida TOL plasmid, pDK1.","authors":"J A Voss, H Khedairy, R F Baker, R C Benjamin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A 5.2 kilobase EcoRI restriction fragment from the Pseudomonas putida HS1 TOL plasmid pDK1, encoding a portion of the lower toluene degradation pathway, was cloned into the E. coli plasmid pBR325. A detailed map of the restriction endonuclease sites was constructed and the nucleotide sequence of three contiguous XhoI fragments, with a combined total length of approximately 3.9 kilobases, has been investigated. This region was determined to contain a total of four separate open reading frames, each preceded by an identical putative ribosome-binding site (nucleotide sequence of 5'-GAGGTG-3'). These open reading frames have been tentatively identified as encoding the lower pathway enzymes catechol 2,3-dioxygenase (C23O) and 1,2-dihydroxycyclohexa-3,5-diene carboxylate dehydrogenase (DHCDH) and a subunit of the toluate 1,2-dioxygenase complex (TO).</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"3 ","pages":"54-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12539993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DNA sequence analysis of an abl-related gene in the blow fly, Calliphora erythrocephala.","authors":"D S Durica, C E Robinson, R B Voelker","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The Ceabl locus is a member of a small family of abl-related sequences in C. erythrocephala. The catalytic, SH2 and SH3 domains of the Ceabl-encoded protein show greater than 75% sequence identity to vertebrate abl protein tyrosine kinases and greater than 95% identity to the D. melanogaster abl polypeptide. Ceabl diverges from the vertebrate proteins, however, at the extreme amino terminus, near the beginning of the vertebrate abl exon involved in differential splicing. The carboxyl region shows no detectable similarity to the vertebrate abl proteins, while identity to Drosophila abl falls to 55%. Regions conserved between the two dipteran genes revealed no strong similarities to other proteins in the Genbank and EMBL databases.</p>","PeriodicalId":77373,"journal":{"name":"SAAS bulletin, biochemistry and biotechnology","volume":"3 ","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12540831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}