PCR methods and applications最新文献_第9页

Development of a sensitive PCR to detect allele loss in a model hematopoietic neoplasm. 一种检测模型造血肿瘤中等位基因丢失的灵敏PCR方法的建立。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.6

J Fairman, D Claxton, C L Williman, A B Deisseroth, L Nagarajan

引用次数: 10

Comparison of gel matrices for resolving PCR-amplified DNA fingerprint profiles. 凝胶基质用于pcr扩增DNA指纹图谱的比较。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.50

G He, C S Prakash, R L Jarret, S Tuzun, J Qiu

引用次数: 15

Protocols for trapping internal and 3'-terminal exons. 捕获内部和3'端外显子的协议。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.s24

P E Nisson, A Ally, P C Watkins

引用次数: 6

A novel method to quantitate methylation of specific genomic regions. 一种量化特定基因组区域甲基化的新方法。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.26

M Heiskanen, A C Syvänen, H Siitari, S Laine, A Palotie

引用次数: 10

Computing genetic similarity coefficients from RAPD data: the effects of PCR artifacts. 从RAPD数据计算遗传相似系数:PCR伪影的影响。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.31

W F Lamboy

{"title":"Computing genetic similarity coefficients from RAPD data: the effects of PCR artifacts.","authors":"W F Lamboy","doi":"10.1101/gr.4.1.31","DOIUrl":"https://doi.org/10.1101/gr.4.1.31","url":null,"abstract":"<p><p>Random amplified polymorphic DNA (RAPD) markers have been used for many types of genetic analyses, including genome mapping, genotype fingerprinting, phylogeny reconstruction, and measuring genetic similarities. They suffer from one potential limitation, however, because the PCR that is used to produce informative amplification products often produces artifactual products as well. Optimization of PCR protocols to eliminate artifactual bands completely is often too costly or too time-consuming to be practical. Other methods for handling RAPD artifacts, such as deleting inconsistent or faint bands or using only those bands that are reproducible, introduce false negatives into the data. Simply ignoring artifacts and using all bands introduces false positives. When RAPD data are used to compute genetic similarity coefficients, such artifacts can cause significant bias in the estimation. The three coefficients most widely used with RAPD data, the simple matching coefficient, Jaccard's coefficient and Nei and Li's coefficient, differ in the amount of bias produced by a given level of artifactual bands. The simple matching coefficient and Nei and Li's coefficient always exhibit less percent bias than Jaccard's coefficient. For closely related organisms, Nei and Li's coefficient displays less percent bias than the simple matching coefficient. If new DNA samples possessing RAPD markers not present in the previously analyzed samples are added to a study, values of the simple matching coefficient will need to be computed for all samples, not just the new ones. Jaccard's and Nei and Li's coefficients, however, will not need to be recomputed. Furthermore, only Nei and Li's coefficient has a direct biological meaning (it is an estimate of the expected proportion of amplified fragments shared by two samples because they were inherited from a common ancestor). On the basis of these results, Nei and Li's coefficient is recommended for routine computation of genetic similarities using RAPD data, particularly if PCR artifacts are present.</p>","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"31-7"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/gr.4.1.31","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 124

PCR-based method to map the bending locus of DNA molecules. 基于pcr的DNA分子弯曲位点定位方法。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.44

F Valle

引用次数: 3

Reverse transcriptase can inhibit PCR and stimulate primer-dimer formation. 逆转录酶可以抑制PCR并刺激引物二聚体的形成。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.62

K M Chumakov

引用次数: 45

A new diagnostic test for Gaucher disease suitable for population screening. 一种适合人群筛查的戈谢病新诊断方法。

PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.1

S A Sakallah, C Sansieri, D W Kopp, D L Cooper, J A Barranger