PCR methods and applications最新文献

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A nonisotopic single-strand conformation polymorphism protocol using a direct blotting electrophoresis, a chemiluminescent detection system, and a multiplex approach. 使用直接印迹电泳,化学发光检测系统和多重方法的非同位素单链构象多态性协议。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.52
H Knoblauch, N Weiss, I Eggersdorfer, C Keller, H Schuster
{"title":"A nonisotopic single-strand conformation polymorphism protocol using a direct blotting electrophoresis, a chemiluminescent detection system, and a multiplex approach.","authors":"H Knoblauch, N Weiss, I Eggersdorfer, C Keller, H Schuster","doi":"10.1101/gr.4.1.52","DOIUrl":"https://doi.org/10.1101/gr.4.1.52","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"52-5"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Strategies for direct sequencing of PCR-amplified DNA. pcr扩增DNA的直接测序策略。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.s15
V B Rao
{"title":"Strategies for direct sequencing of PCR-amplified DNA.","authors":"V B Rao","doi":"10.1101/gr.4.1.s15","DOIUrl":"https://doi.org/10.1101/gr.4.1.s15","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"S15-23"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
On beyond classic RACE (rapid amplification of cDNA ends). 超越经典的RACE (cDNA末端快速扩增)。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.s40
M A Frohman
{"title":"On beyond classic RACE (rapid amplification of cDNA ends).","authors":"M A Frohman","doi":"10.1101/gr.4.1.s40","DOIUrl":"https://doi.org/10.1101/gr.4.1.s40","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"S40-58"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 174
Advanced methods in PCR product detection. PCR产物检测的先进方法。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.s1
J G Lazar
{"title":"Advanced methods in PCR product detection.","authors":"J G Lazar","doi":"10.1101/gr.4.1.s1","DOIUrl":"https://doi.org/10.1101/gr.4.1.s1","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"S1-14"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/gr.4.1.s1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
Determining relative microsatellite allele frequencies in pooled DNA samples. 测定混合DNA样本中相对微卫星等位基因频率。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.13
H Khatib, A Darvasi, Y Plotski, M Soller
{"title":"Determining relative microsatellite allele frequencies in pooled DNA samples.","authors":"H Khatib,&nbsp;A Darvasi,&nbsp;Y Plotski,&nbsp;M Soller","doi":"10.1101/gr.4.1.13","DOIUrl":"https://doi.org/10.1101/gr.4.1.13","url":null,"abstract":"<p><p>Accurate quantification of relative allele frequencies in pooled DNA samples can be carried out for microsatellite markers having a dinucleotide repeat unit, conditional on the absence of overlapping \"shadow\" bands. This provides a basis for extending DNA pooling to this useful class of DNA marker. Expressions for the standard error of densitometric estimates of allele frequencies from pooled samples are presented, and their statistical application is illustrated in a variety of situations. This enables DNA pooling to be utilized in situations requiring the testing of statistical hypotheses concerning differences in allele frequencies between populations, or samples.</p>","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"13-8"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 45
A simple PCR method for screening cDNA libraries. 一种筛选cDNA文库的简易PCR方法。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.46
D Alfandari, T Darribère
{"title":"A simple PCR method for screening cDNA libraries.","authors":"D Alfandari,&nbsp;T Darribère","doi":"10.1101/gr.4.1.46","DOIUrl":"https://doi.org/10.1101/gr.4.1.46","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"46-9"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1101/gr.4.1.46","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Buffer components tailor DNA amplification with arbitrary primers. 用任意引物进行 DNA 扩增的定制缓冲液成分。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.59
G Caetano-Anollés, B J Bassam, P M Gresshoff
{"title":"Buffer components tailor DNA amplification with arbitrary primers.","authors":"G Caetano-Anollés, B J Bassam, P M Gresshoff","doi":"10.1101/gr.4.1.59","DOIUrl":"10.1101/gr.4.1.59","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"59-61"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
PCR with end trimming and cassette ligation: a rapid method to clone exon-intron boundaries and a 5'-upstream sequence of genomic DNA based on a cDNA sequence. 带有末端修剪和盒连接的PCR:一种基于cDNA序列克隆外显子-内含子边界和基因组DNA 5'上游序列的快速方法。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.19
H Iwahana, T Tsujisawa, R Katashima, K Yoshimoto, M Itakura
{"title":"PCR with end trimming and cassette ligation: a rapid method to clone exon-intron boundaries and a 5'-upstream sequence of genomic DNA based on a cDNA sequence.","authors":"H Iwahana,&nbsp;T Tsujisawa,&nbsp;R Katashima,&nbsp;K Yoshimoto,&nbsp;M Itakura","doi":"10.1101/gr.4.1.19","DOIUrl":"https://doi.org/10.1101/gr.4.1.19","url":null,"abstract":"<p><p>We described a method for PCR amplification of unknown flanking genomic DNA fragments. This method is a combination of PCR with \"end-trimming method\" and \"cassettes and cassette-primers method\". In this method, genomic DNA was digested with three different groups of restriction enzymes. DNA in group 1 was digested with BamHI, BglII, FbaI, or MboI. DNA in group 2 was digested with BlnI, NheI, SpeI, or XbaI. DNA in group 3 was digested with SalI or XhoI. Digested DNA in each group was end-trimmed with Klenow fragment of DNA polymerase I in the presence of only one dNTP; dGTP, dCTP, and dTTP for group 1, 2, and 3, respectively. The synthesized cassettes, C1, C2, and C3, had 5'protruding sequences of 5'-ATC-3',5'-TAG-3', and 5'-CGA-3', respectively. Each compatible cassette was ligated to the end-trimmed DNAs in group 1-3, respectively. Nested PCR was then performed using an end-trimmed and cassette-ligated DNA as a template. Primers annealing to known sequences and cassettes were used for the nested PCR. The amplified DNA fragments were electrophoresed on a polyacrylamide gel and purified. The sequences of the DNA fragments were determined after cloning into pBluescript.</p>","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"19-25"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 18
A DNA extraction method that allows reliable PCR amplification of MLO DNA from "difficult" plant host species. 一种DNA提取方法,可以从“困难”的植物宿主物种中可靠地扩增MLO DNA。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.56
K Gibb, A Padovan
{"title":"A DNA extraction method that allows reliable PCR amplification of MLO DNA from \"difficult\" plant host species.","authors":"K Gibb,&nbsp;A Padovan","doi":"10.1101/gr.4.1.56","DOIUrl":"https://doi.org/10.1101/gr.4.1.56","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"56-8"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19977483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 24
DNA fingerprinting by arbitrarily primed PCR. 任意引物PCR的DNA指纹图谱。
PCR methods and applications Pub Date : 1994-08-01 DOI: 10.1101/gr.4.1.s59
M McClelland, J Welsh
{"title":"DNA fingerprinting by arbitrarily primed PCR.","authors":"M McClelland,&nbsp;J Welsh","doi":"10.1101/gr.4.1.s59","DOIUrl":"https://doi.org/10.1101/gr.4.1.s59","url":null,"abstract":"","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 1","pages":"S59-65"},"PeriodicalIF":0.0,"publicationDate":"1994-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19979419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 91
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