Molecular marine biology and biotechnology最新文献_第5页

An efficient DNA extraction method for small metazoans. 小型后生动物一种高效的DNA提取方法。

Molecular marine biology and biotechnology Pub Date : 1997-12-01

N V Schizas, G T Street, B C Coull, G T Chandler, J M Quattro

引用次数: 0

Characterization of cell cultures derived from Fugu, the Japanese pufferfish. 日本河豚细胞培养物的特性。

Molecular marine biology and biotechnology Pub Date : 1997-12-01

C S Bradford, A E Miller, A Toumadje, K Nishiyama, S Shirahata, D W Barnes

{"title":"Characterization of cell cultures derived from Fugu, the Japanese pufferfish.","authors":"C S Bradford, A E Miller, A Toumadje, K Nishiyama, S Shirahata, D W Barnes","doi":"","DOIUrl":"","url":null,"abstract":"The Japanese pufferfish (genus Fugu), which possesses a highly compact genome, is becoming a popular model among those interested in sequencing and mapping the genomes of higher vertebrates. Although genomic libraries have been derived and used to study the molecular biology of Fugu, biological material derived from the living organism is difficult to obtain for laboratories distant from the Asian Pacific. We have established cell cultures from two Fugu species: kusafugu, Fugu niphobles, and torafugu, F. rubripes. Cultures derived from F. niphobles fry and F. rubripes eye have been passaged more than 60 times over the course of one year, representing approximately 180 population doublings. Proliferating cultures were also initiated from F. rubripes brain, liver, fin, spleen, kidney, swimbladder, and muscle. Karyotype analyses indicated that F. rubripes eye-derived cells possessed a chromosome number in the diploid range; F. niphobles fry cells were slightly hyperploid. Flow cytometry confirmed that the relative amounts of DNA present in cultured cells from both Fugu species were similar to that measured in blood cells collected from F. rubripes, and approximately one-seventh of that measured in diploid human cells. Telomerase activity was easily detectable in lysates prepared from F. niphobles fry cells and F. rubripes eye cells, consistent with the notion that these cultures are capable of indefinite proliferation.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"6 4","pages":"279-88"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20345615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A functional study of the salmon GnRH promoter. 鲑鱼GnRH启动子的功能研究。

Molecular marine biology and biotechnology Pub Date : 1997-12-01

H Husebye, P Collas, P Aleström

引用次数: 0

Cyclins in a dinoflagellate cell cycle. 鞭毛藻细胞周期中的周期蛋白。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

A Leveson, F Wong, J T Wong

{"title":"Cyclins in a dinoflagellate cell cycle.","authors":"A Leveson, F Wong, J T Wong","doi":"","DOIUrl":"","url":null,"abstract":"The dinoflagellates are distinct eukaryotes in having and extranuclear spindle and permanently condensed chromosomes. These cytologic features implicate special adaptations to the molecular mechanisms of cell cycle control. We have demonstrated the presence of cyclin-box-containing polypeptides in dinoflagellates by immunoblotting using peptide-generated antibodies. We identified four major cyclin-box-containing polypeptides. The cell cycle dynamics of these polypeptides were also investigated in synchronized populations, using a newly developed method. Of the four major cyclin-box-containing polypeptides detected, a triplex with apparent molecular weight of 75 kDa did not change appreciably during the cell cycle. For two other cyclin-box-containing polypeptides with apparent molecular weights of 50 and 65 kDa, we observed an early expression in the cell cycle, with the level accumulating and eventually being degraded on the exit of mitosis. At least on cyclin-box-containing polypeptide (50 kDa) was also observed in a protein complex bound to p13suc1 beads. The bound complex head associated histone kinase activity. Variation of this activity corresponded well with the periodic expression of the 50-kDa cyclin-box-containing polypeptide during the cell cycle of Crypthecodinium cohnii. This demonstrates the presence of cyclins and cyclin-dependent kinases in dinoflagellates.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"6 3","pages":"172-9"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20226397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribosomal RNA gene dosage in marine bacteria. 海洋细菌核糖体RNA基因用量。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

L Kerkhof, M Speck

引用次数: 0

Conservation of the myoglobin gene among Antarctic notothenioid fishes. 南极南极南极南极鱼类肌红蛋白基因的保护。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

M E Vayda, D J Small, M L Yuan, L Costello, B D Sidell

{"title":"Conservation of the myoglobin gene among Antarctic notothenioid fishes.","authors":"M E Vayda, D J Small, M L Yuan, L Costello, B D Sidell","doi":"","DOIUrl":"","url":null,"abstract":"We determined the myoglobin cDNA sequence for seven Antarctic notothenioid fish species. These data identify mutations in the myoglobin gene for Champsocephalus gunnari and Pagetopsis macropterus, two icefish species that lack detectable quantities of the polypeptide but express myoglobin mRNA. a third species lacking myoglobin polypeptide, Chaenocephalus aceratus, is devoid of myoglobin mRNA and accordingly failed to produce myoglobin products on polymerase chain reaction (PCR) amplification. Myoglobin cDNA sequences were highly conserved among the species the express the protein, particularly in the coding region. Sequence variation among the myoglobin-expressing channichthyid species was 2.0% to 2.9% in the coding region and 2.6% to 3.3% over the entire cDNA. The same extent of variation, 1.6% to 3.2% in the coding sequence and 2.8% to 3.7% overall, was observed between the icefishes and more distantly related, red-blooded nototheniid species. The two species expressing mutant myoglobin mRNA, C. gunnari and P. macropterus, exhibited the highest degree of sequence variation among the fish myoglobins examined. Drift in the myoglobin sequence in these two species, and conservation of myoglobin cDNA among fishes from two distinct families, suggest that a selective pressure operates to maintain myoglobin in the species that express the protein.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"6 3","pages":"207-16"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20226399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gonadotropin alpha-subunit glycoprotein from channel catfish (Ictalurus punctatus) and its expression during hormone-induced ovulation. 通道鲶鱼促性腺激素亚基糖蛋白及其在激素诱导排卵中的表达。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

Z Liu, P Li, B J Argue, R A Dunham

{"title":"Gonadotropin alpha-subunit glycoprotein from channel catfish (Ictalurus punctatus) and its expression during hormone-induced ovulation.","authors":"Z Liu, P Li, B J Argue, R A Dunham","doi":"","DOIUrl":"","url":null,"abstract":"Complementary DNA (cDNA) encoding the channel catfish (Ictalurus punctatus) gonadotropin (GTH) alpha-subunit glycoprotein was cloned by polymerase chain reaction (PCR) from a plasmid library made from pituitary RNA. Complete cDNA cloning was achieved by carrying out two PCR reactions: one with an upstream sense primer plus the universal sequencing primer, located downstream of the poly(A) sequence of the cDNA in the plasmid vector, to amplify the downstream portion of the cDNA; the other with a downstream antisense primer plus the reverse-sequencing primer, located upstream of the very 5' end of the cDNA sense strand in the plasmid vector, to amplify the upstream portion of the cDNA. The two amplified fragments overlapping about 70 bp. Nucleotide sequence analysis revealed that the catfish GTH alpha-subunit was 658 bp encoding 116 amino acids and harboring a 5' nontranslated region (NTR) of 42 bp and a 3' NTR of 265 bp. The deduced amino acid sequence of the catfish GTH alpha-subunit is highly conserved with those from other cloned teleost GTH alpha-subunits. The GTH alpha-subunit was highly expressed even before induction for ovulation in females during spawning season. Administration of carp pituitary extract (a spawning-inducing reagent) induced only 1.4-fold higher expression of the GTH alpha-subunit RNA, but included very rapid egg maturation and ovulation. This unexpected result indicated that the GTH alpha-subunit may not be the limiting factor for ovulation and spawning, which may be regulated by the change of proportional coupling of the GTH alpha-subunit with specific beta-subunit during hormone-induced ovulation.","PeriodicalId":77273,"journal":{"name":"Molecular marine biology and biotechnology","volume":"6 3","pages":"217-27"},"PeriodicalIF":0.0,"publicationDate":"1997-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20226400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alteration in plasmid DNA following natural transformation to populations of marine bacteria. 自然转化为海洋细菌群体后质粒DNA的改变。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

H G Williams, J Benstead, M E Frischer, J H Paul

引用次数: 0

Molecular phylogenetics of bacterial endosymbionts and their vestimentiferan hosts. 细菌内共生体及其前体宿主的分子系统发育。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

R A Feldman, M B Black, C S Cary, R A Lutz, R C Vrijenhoek

引用次数: 0

Amylase on Pecten maximus (Mollusca, bivalves): protein and cDNA characterization; quantification of the expression in the digestive gland. 大Pecten maximus(软体动物，双壳类)淀粉酶的蛋白质和cDNA鉴定在消化腺中定量表达。

Molecular marine biology and biotechnology Pub Date : 1997-09-01

S Le Moine, D Sellos, J Moal, J Y Daniel, F San Juan Serrano, J F Samain, A Van Wormhoudt

引用次数: 0