S Le Moine, D Sellos, J Moal, J Y Daniel, F San Juan Serrano, J F Samain, A Van Wormhoudt
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引用次数: 0
Abstract
The digestive enzyme alpha-amylase in Pecten maximus has been purified from the digestive gland, where it is present as two isoforms, In order to gain information on its structure and regulation, a digestive gland cDNA library, constructed in lambda phage Zap II (Stratagene, La Jolla, Calif., U.S.A.), was screened with a shrimp alpha-amylase cDNA probe. Only 0.02% of the clones were positive, and the longest clone, having a size of 1700 bp and identical to that of the mRNA, was fully sequenced. It contains the complete cDNA coding frame for one of the amylase isoforms of P. maximus. The deduced protein sequence is 508 amino acids long, with a putative 18 amino acid, highly hydrophobic signal peptide and a mature enzyme of 489 residues. The molecular weight corresponds to 54,500 Da and the calculated isoelectric point is 6.76. Locations of conserved sequences confirms the high level of similarity with the other members of the family.
为了获得其结构和调控的信息，我们用虾α -淀粉酶cDNA探针在lambda噬菌体Zap II (Stratagene, La Jolla, Calif. U.S.A.)中构建了一个消化腺cDNA文库。只有0.02%的克隆呈阳性，最长的克隆全长为1700bp，与mRNA的长度相同。它包含了p.a maximus一种淀粉酶同工型的完整cDNA编码框。该蛋白序列长508个氨基酸，推测含有18个氨基酸，高度疏水的信号肽和489个残基的成熟酶。分子量为54,500 Da，等电点为6.76。保守序列的位置证实了与家族其他成员的高度相似性。
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