Lymphokine and cytokine research最新文献_第7页

Strain differences in the severity of lesions in murine systemic candidiasis correlate with the production of functional gamma interferon by Candida-activated lymphocytes in vitro. 小鼠全身念珠菌病病变严重程度的品系差异与体外念珠菌激活淋巴细胞产生功能性γ干扰素有关。

Lymphokine and cytokine research Pub Date : 1993-12-01

R B Ashman, E M Bolitho

{"title":"Strain differences in the severity of lesions in murine systemic candidiasis correlate with the production of functional gamma interferon by Candida-activated lymphocytes in vitro.","authors":"R B Ashman, E M Bolitho","doi":"","DOIUrl":"","url":null,"abstract":"BALB/c mice exhibit mild, and CBA/CaH mice exhibit severe tissue lesions after systemic infection with the yeast Candida albicans. There is considerable evidence that recovery from primary infection is associated with the development of cell-mediated immune responses in the infected mice, and that cytokines produced by T lymphocytes augment the phagocytic and candidacidal functions of macrophages and polymorphonuclear leukocytes. To determine whether the severity of lesions in the two inbred strains was associated with differences in cytokine production, lymphocytes were obtained from the spleens of normal and immune mice, activated with Candida antigens in vitro, and functional cytokines identified by bioassay. mRNA obtained from the activated lymphocytes was also screened for cytokine-specific sequences by S1-nuclease protection analysis. mRNA for IL-3 and IFN-gamma, but not IL-2 or IL-4, was detected in lymphocytes from both BALB/c and CBA/CaH mice, but only lymphocytes from primary and secondary cultures of BALB/c cells showed Candida-specific induction of IFN-gamma bioactivity. These results suggest that, in this murine model of systemic candidiasis, the propensity to develop severe lesions exhibited by CBA/CaH mice may be a consequence of some form of posttranscriptional regulation of gamma-interferon production.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"12 6","pages":"471-6"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19117219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human granulocyte colony-stimulating factor (G-CSF), the premier granulopoietin: biology, clinical utility, and receptor structure and function. 人粒细胞集落刺激因子(G-CSF)，主要的粒细胞生成素:生物学，临床应用，受体结构和功能。

Lymphokine and cytokine research Pub Date : 1993-12-01

L S Tkatch, D J Tweardy

引用次数: 0

Cytostatic and cytotoxic effects of tumor necrosis factor alpha on MCF-7 human breast tumor cells are differently inhibited by glucocorticoid hormones. 肿瘤坏死因子α对MCF-7人乳腺肿瘤细胞的细胞抑制和细胞毒性作用受到糖皮质激素不同程度的抑制。

Lymphokine and cytokine research Pub Date : 1993-12-01

M C Pagliacci, G Fumi, G Migliorati, F Grignani, C Riccardi, I Nicoletti

{"title":"Cytostatic and cytotoxic effects of tumor necrosis factor alpha on MCF-7 human breast tumor cells are differently inhibited by glucocorticoid hormones.","authors":"M C Pagliacci, G Fumi, G Migliorati, F Grignani, C Riccardi, I Nicoletti","doi":"","DOIUrl":"","url":null,"abstract":"To investigate the mechanisms of growth inhibition exerted by TNF-alpha on tumor cells in vitro, we analyzed the cytokine effects on growth and cell-cycle parameters of cultured MCF-7 human breast cancer cells. TNF-alpha exerted a dose-dependent inhibition of MCF-7 cell growth, which reached its maximum at 1000 U/ml TNF-alpha concentrations. Flow-cytometric analysis of cell nuclei revealed two main components in TNF-alpha activity: an earlier cytostatic effect (G1/S block), was followed by nuclear shrinkage and cytolysis. The 55-60-kDa TNF-alpha receptor is involved in the growth inhibitory activity of the cytokine, since the H398 anti-55-kDa receptor antibody significantly counteracted the cytostatic and cytotoxic effects of TNF-alpha while an antibody (htr-9) with agonistic activity on the same receptor produced both cytostasis and cytolysis. Culture conditions strongly influenced the MCF-7 cell response to TNF-alpha. Serum deprivation of log-growing (i.e., high S phase percentage) cultures potentiated the cytotoxic effect, while reduction in S phase cell percentage by preculture in serum-free medium resulted in a significant inhibition of TNF-alpha action. Mitogenic hormones, such as insulin and 17 beta-estradiol+insulin, restored the sensitivity of MCF-7 cells precultured in serum-free medium to both the cytostatic and cytolytic effects of TNF-alpha. The synthetic glucocorticoid hormone dexamethasone, at micromolar concentrations, counteracted the TNF-alpha effect on MCF-7 cell growth. Flow-cytometric analysis showed that dexamethasone did not antagonize the cytostatic activity of either TNF-alpha or htr-9 agonistic antibody, but only the subsequent cytolysis.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"12 6","pages":"439-47"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19115866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of recombinant human interleukin-6 on blood and immune parameters in middle-aged and old rhesus monkeys. 重组人白细胞介素-6对中老年恒河猴血液及免疫指标的影响。

Lymphokine and cytokine research Pub Date : 1993-12-01

W H Sun, N Binkley, D W Bidwell, W B Ershler

{"title":"The influence of recombinant human interleukin-6 on blood and immune parameters in middle-aged and old rhesus monkeys.","authors":"W H Sun, N Binkley, D W Bidwell, W B Ershler","doi":"","DOIUrl":"","url":null,"abstract":"IL-6 is a 26-kDa protein cytokine with pleiotropic activities in both hematopoietic and immune systems. It is one of the major mediators of the acute phase inflammatory response. Recently it has been demonstrated that pharmacological doses of human recombinant IL-6 (rhIL-6) inhibit certain murine tumors as well as stimulate thrombopoiesis in mice, dogs, nonhuman primates, and humans. The purpose of our study was to evaluate the effects and toxicity of rhIL-6 administration in nonhuman primates with particular reference to subject age. We treated 10 female monkeys of two age groups (midle-aged and old) with rhIL-6 (15 micrograms/kg/day) for 28 days. The monkeys were observed to be somewhat lethargic and lost an average of 10% of their body weights. The white blood cell count rose transiently whereas the levels of hemoglobin and hematocrit fell significantly and remained depressed for the same period. Importantly, platelet count rose and remained elevated for the duration of treatments. Serum alkaline phosphatase levels increase significantly and certain parameters of clinical immune competence were altered by IL-6 treatment. Treatment effects were similar in both age groups, but the changes in immune functions were different between the midle-aged and old monkeys. We observed a pattern in which the middle-aged group had a significant decrease in immune functions as a result of IL-6 administration and recovered to pretreatment level despite continuous treatment, whereas the old monkeys had a more protracted but less significant decline in these same immune functions during the trial.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"12 6","pages":"449-55"},"PeriodicalIF":0.0,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19115869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of lymphotoxin in the IL-2-driven differentiation of human lymphokine-activated T-killer (T-LAK) cells in vitro. 淋巴蛋白在体外人淋巴因子激活的t -杀伤细胞(T-LAK) il -2驱动分化中的作用。