Lymphokine and cytokine research最新文献_第10页

Detection of oncostatin M in human plasma and serum by a sensitive enzyme immunoassay. 用灵敏的酶免疫分析法检测人血浆和血清中抑癌素M。

Lymphokine and cytokine research Pub Date : 1993-06-01

J R Naemura, S F Radka

引用次数: 0

Studies on the biological effects of ozone: 3. An attempt to define conditions for optimal induction of cytokines. 臭氧的生物效应研究:试图确定细胞因子诱导的最佳条件。

Lymphokine and cytokine research Pub Date : 1993-04-01

V Bocci, E Luzzi, F Corradeschi, L Paulesu, A Di Stefano

引用次数: 0

Contrasting effects of interleukin-4 on colony-stimulating factor and interleukin-6 synthesis by vascular endothelial cells. 白细胞介素-4对血管内皮细胞集落刺激因子和白细胞介素-6合成的影响。

Lymphokine and cytokine research Pub Date : 1993-04-01

H Zoellner, J Cebon, J E Layton, H Stanton, J A Hamilton

{"title":"Contrasting effects of interleukin-4 on colony-stimulating factor and interleukin-6 synthesis by vascular endothelial cells.","authors":"H Zoellner, J Cebon, J E Layton, H Stanton, J A Hamilton","doi":"","DOIUrl":"","url":null,"abstract":"Endothelial cells (EC) may regulate both local and systemic aspects of inflammation through the synthesis of cytokines such as granulocyte-macrophage colony-stimulating factor (GM-CSF), granulocyte colony-stimulating factor (G-CSF), macrophage colony-stimulating factor (M-CSF), and interleukin-6 (IL-6). EC are known to synthesize these cytokines in response to interleukin-1 (IL-1 alpha), tumor necrosis factor-alpha (TNF-alpha) and lipopolysaccharide (LPS). In this paper, we illustrate the effect of interleukin-4 (IL-4) in reducing the synthesis of GM-CSF by EC stimulated with IL-1 alpha, TNF-alpha, or LPS. This is compared with the previously reported strong synergy between IL-4 and IL-1 alpha, TNF-alpha, or LPS in the synthesis of IL-6 by EC. No clear effect of IL-4 was seen in the synthesis of G-CSF or M-CSF. The range of concentrations of IL-4 at which these effects were seen was identical for both reduced GM-CSF synthesis and increased IL-6 synthesis. The effect of IL-4 on IL-6 synthesis was seen by 4 h of treatment, while that on GM-CSF was apparent between 4 and 8 h. It is suggested that these contrasting effects of IL-4 may reflect a biological role for this cytokine in the regulation of leukocytosis and the acute phase response.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"12 2","pages":"93-9"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19310886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of gamma-interferon on collagen and histamine content in bleomycin-induced lung fibrosis in rats. γ -干扰素对博莱霉素诱导大鼠肺纤维化中胶原蛋白和组胺含量的影响。

Lymphokine and cytokine research Pub Date : 1993-04-01

T Okada, I Sugie, K Aisaka

引用次数: 0

Cytokine kinetics in an in vitro whole blood model following an endotoxin challenge. 内毒素刺激后体外全血模型的细胞因子动力学。

Lymphokine and cytokine research Pub Date : 1993-04-01

J C Oliver, L A Bland, C W Oettinger, M J Arduino, S K McAllister, S M Aguero, M S Favero

引用次数: 0

A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples. 从小细胞样本中基于pcr的多种细胞因子基因转录物半定量的标准化方法。

Lymphokine and cytokine research Pub Date : 1993-04-01

S Lagoo-Deenadayalan, A S Lagoo, W H Barber, K J Hardy

{"title":"A standardized approach to PCR-based semiquantitation of multiple cytokine gene transcripts from small cell samples.","authors":"S Lagoo-Deenadayalan, A S Lagoo, W H Barber, K J Hardy","doi":"","DOIUrl":"","url":null,"abstract":"A simple, rapid, reproducible, and nonradioisotopic method for semiquantitative analysis of cytokine mRNAs based on polymerase chain reaction (PCR) is described. RNA isolated from 2.5 million cells has proven sufficient to perform semiquantitative analysis of mRNA for 10 different cytokines. By this approach accurate assessment of mRNA levels for multiple cytokines can be made from as little as 2 ml of blood or about 3 mg of biopsy material. Total cellular RNA is quantitatively recovered by guanidinium isothiocyanate-acid-phenol extraction of a constant number of cells. Further quantitation of RNA is unnecessary. Highly reproducible PCR product formation occurs after specific amplification of aliquots of reverse transcribed test RNA. The photographic image of the ethidium bromide-stained gel accurately reflects the amount of PCR product loaded, both densitometrically and visually. PCR product generation is not affected by the presence of carrier RNA. Thus quantitative recovery of total RNA is possible even from a very small number of cells. Similarly, presence of a large excess of nonspecific RNA from nonexpressing cells does not affect amplification of the specific mRNA under study. A linear relationship between mRNA frequency and PCR product formation is observed over a 256- to 512-fold range. The actual mRNA concentration for each cytokine varies depending on the relative abundance of mRNA for that cytokine relative to total RNA. By performing two amplification cycles (28 and 35) on undiluted and 10-fold diluted RNA samples, the range of detection linearity is extended over a 5000-fold difference in input RNA levels.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"12 2","pages":"59-67"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19310267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization and production of human macrophage colony-stimulating factor (hM-CSF) in human placental and decidual tissues. 人巨噬细胞集落刺激因子(hM-CSF)在人胎盘和蜕膜组织中的定位和产生。

Lymphokine and cytokine research Pub Date : 1993-04-01

S Saito, K Motoyoshi, M Saito, Y Kato, M Enomoto, K Nishikawa, T Morii, M Ichijo

{"title":"Localization and production of human macrophage colony-stimulating factor (hM-CSF) in human placental and decidual tissues.","authors":"S Saito, K Motoyoshi, M Saito, Y Kato, M Enomoto, K Nishikawa, T Morii, M Ichijo","doi":"","DOIUrl":"","url":null,"abstract":"The localization of the human macrophage colony-stimulating factor (hM-CSF) in human trophoblast cells (syncytiotrophoblast and cytotrophoblast), decidual stromal cells, decidual lymphocytes, macrophages, and endometrial gland cells during the early stage of pregnancy was determined by the immunohistochemical examination and in situ hybridization. A large amount of hM-CSF was detected in the supernatant of the primary cultured cytotrophoblasts and decidual stromal cells. The decidual stromal cells secreted approximately 2 to 20 times as much hM-CSF as the trophoblast cells. Northern blot analysis showed that the placental and decidual tissues expressed the 4.0 kb hM-CSF-mRNA, however a larger amount of hM-CSF-related mRNA was found in the decidual than in the placental tissue. We also demonstrated the localization of hM-CSF mRNA expression in human decidual macrophages and CD16- CD56+ NK cells using the RT-PCR method. These findings indicate that hM-CSF is produced by decidual stromal cells, decidual macrophages, decidual CD16- CD56+ NK cells, and endometrial gland cells, as well as by trophoblasts. This increased production of hM-CSF may play a role in decidual function and placental function by the autocrine and paracrine system.","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"12 2","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"1993-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18690178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of gene expression of myeloperoxidase (MPO) by gamma-interferon (IFN-gamma) in HL60 cells. γ干扰素(ifn - γ)对HL60细胞髓过氧化物酶(MPO)基因表达的抑制作用

Lymphokine and cytokine research Pub Date : 1993-04-01

S Kawano, E Tatsumi, N Yoneda, S Nagata, N Yamaguchi

引用次数: 0

The role of iron in the cytotoxicity of tumor necrosis factor. 铁在肿瘤坏死因子细胞毒性中的作用。

Lymphokine and cytokine research Pub Date : 1993-04-01

S Warren, S V Torti, F M Torti

引用次数: 0

The enhancement of human tumor necrosis factor-alpha antiviral activity in vivo by monoclonal and specific polyclonal antibodies. 单克隆和特异性多克隆抗体增强人肿瘤坏死因子- α体内抗病毒活性。

Lymphokine and cytokine research Pub Date : 1993-04-01

B A Lidbury, R Aston, I A Ramshaw, W B Cowden, D A Rathjen

引用次数: 0