γ干扰素(ifn - γ)对HL60细胞髓过氧化物酶(MPO)基因表达的抑制作用

Lymphokine and cytokine research Pub Date : 1993-04-01
S Kawano, E Tatsumi, N Yoneda, S Nagata, N Yamaguchi
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引用次数: 0

摘要

髓过氧化物酶(MPO)基因在HL60细胞中的表达,用MPO基因探针检测,在加入γ -干扰素(ifn - γ) (200 U/ml) 9 h后,MPO基因表达明显被抑制,而细胞化学检测MPO活性、细胞表面抗原表达、50 U/ml的ifn - γ足以抑制24小时的MPO基因。用200 U/ml的ifn - γ处理24小时的HL60细胞在没有ifn - γ的情况下再培养24小时,MPO基因的转录恢复到与完全没有ifn - γ培养的HL60细胞相当的水平,表明抑制的可逆性。在RNA水平上抑制MPO表达,可能与分化无关,是ifn - γ发挥的一种生物活性,此前未见报道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Suppression of gene expression of myeloperoxidase (MPO) by gamma-interferon (IFN-gamma) in HL60 cells.

The gene expression of myeloperoxidase (MPO) in HL60 cells, as tested by Northern blot analysis with a MPO gene probe, was markedly suppressed at 9 h after the addition of gamma-interferon (IFN-gamma) (200 U/ml), whereas cytochemically detected MPO activity, cell surface antigen expression, and cell morphology remained unchanged even at 48 h. IFN-gamma of 50 U/ml was sufficient for the suppression at 24 h. When the HL60 cells treated with IFN-gamma (200 U/ml) for 24 h were cultured in the absence of IFN-gamma for another 24 h, the transcript of MPO gene reverted to a level comparable to that of the HL60 cells cultured thoroughly in the absence of IFN-gamma, indicating the reversibility of the suppression. The suppression of MPO expression at RNA level, probably independent of differentiation, is a biological activity exerted by IFN-gamma, which has not been previously reported.

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