{"title":"Detection of tdh and trh genes in a urea-hydrolysing environmental isolate of Vibrio parahaemolyticus from the Andamans.","authors":"A R Ghosh, S C Sehgal","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Co-existence of trh gene and urea-hydrolysing property in one of 44 marine water isolates of Vibrio parahaemolyticus correlates strongly with both genotypic and phenotypic characteristics of the bacterium. Thus, urease-producing phenotype can be considered a marker of virulence for the production of thermostable direct haemolysin-related haemolysin (TRH) (i.e. possession of trh gene). The same isolate also possessed the tdh gene. An environmental isolate possessing all the characteristics of a pathogenic V. parahaemolyticus in this marine environment suggest that there is a likelihood of the occurrence of clinical cases of gastroenteritis caused by V. parahaemolyticus in the Andamans.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 2","pages":"87-90"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20717070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Time series analysis of patients with rotavirus diarrhoea in Pune, India.","authors":"S G Purohit, S D Kelkar, V Simha","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effects of seasonality and other temporal patterns on the occurrence of rotavirus diarrhoea were studied among hospitalised cases at Pune, India from July 1992 to June 1996. The well-accepted Box-Jenkins methodology based on modelling was employed for the analysis. This is the first presentation of such analysis for rotavirus diarrhoea. The model suggests strong influence of climatic changes on the incidence of the disease. Further study of weather parameters not only confirms that daily minimum temperature is the principal factor but also reveals that easterly wave, a characteristic feature of tropical weather, is useful in predicting the peak of hospital admissions and the geographical sequence of outbreaks of the disease in tropical India.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 2","pages":"74-83"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20717068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J Singh, V Sachdeva, R Bhatia, D Bora, D C Jain, J Sokhey
{"title":"Endemic cholera in Delhi, 1995: analysis of data from a sentinel centre.","authors":"J Singh, V Sachdeva, R Bhatia, D Bora, D C Jain, J Sokhey","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Data on cholera cases admitted to the Delhi Infectious Diseases Hospital (IDH) are presented to describe the pattern of occurrence of cholera in Delhi in 1995. Rectal swabs from 4082 cases of acute diarrhoea admitted to the IDH were examined for excretion of Vibrio cholerae. Of them, 2004(49%) and 4(0.1%) were positive for V. cholerae O1 biotype El Tor and V. cholerae O139 respectively. Most cholera cases occurred during May-September (summer and monsoon months). The period from January to March (winter) was completely free from cholera. The urban areas were not affected uniformly. Of the 80 PIN (Postal Index Number) code areas, 10 contributed to 57% of the cases. The early cases were scattered in PIN code areas distant from one another. The hospitalisation rates for cholera were the highest in children aged less than five years and declined significantly with increasing patients' age. Males had significantly higher rates than females aged up to 20 years, whereas the situation was reversed in the 20 to 39 year age group. Four per cent of the affected families had multiple cases. An estimated 1% of the household contacts of hospitalised cases of cholera were themselves hospitalised for cholera within 2 days of the first admission. Of the 260 V. cholerae O1 isolates tested, 4%, 7%, 8%, 89%, 91% and 95% were resistant to tetracycline, nalidixic acid, chloramphenicol, co-trimoxazole, streptomycin, and furazolidone respectively. The study highlights the usefulness of surveillance data to identify groups, urban areas and seasons with increased risk for cholera and to allow control measures to be focussed on those in greatest need.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 2","pages":"66-73"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20717067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D F Souza, J J Kisielius, M Ueda, Y B Gabbay, R C Carmona, M do C St Timenetsky, J D Mascarenhas, S Takimoto, H Tanaka
{"title":"An outbreak of group C rotavirus gastroenteritis among adults living in Valentim Gentil, São Paulo State, Brazil.","authors":"D F Souza, J J Kisielius, M Ueda, Y B Gabbay, R C Carmona, M do C St Timenetsky, J D Mascarenhas, S Takimoto, H Tanaka","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An outbreak of gastroenteritis affecting adults and children occurred in the small city of Valentim Gentil, São Paulo, Brazil, in 1993. Nineteen faecal samples (from 10 cases and 9 contracts) were examined by direct electron microscopy (DEM), immune electron microscopy (IEM), polyacrylamide gel electrophoresis (PAGE), and enzyme-linked immunosorbent assay (ELISA) for group A and C rotaviruses. DEM detected rotavirus in 6 of the 10 cases and in none of the contacts. All of the samples were negative for group A rotavirus by ELISA. Analysis by PAGE showed an electrophoretic profile suggestive of group C rotavirus in two cases. Group C rotavirus was identified by IEM in 4 of the cases and in 1 of the contacts. All of the samples were submitted to ELISA for group C rotavirus. This resulted in a total of 10 positives-7 for diarrhoeal cases and 3 for contacts. This outbreak was strongly associated with group C rotavirus. The importance of combining different diagnostic methods is emphasised.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 2","pages":"59-65"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20717111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Time series and (S)ARMA models.","authors":"E Kestens","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 2","pages":"84-6"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20717069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bibliography on diarrhoeal diseases.","authors":"M S Khan, M Nazimuddin, M al Mamun","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 2","pages":"146-72"},"PeriodicalIF":0.0,"publicationDate":"1998-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20717071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J D Mascarenhas, F L Paiva, C R Barardi, Y B Gabbay, C O Simões, A C Linhares
{"title":"Rotavirus G and P types in children from Belém, northern Brazil, as determined by RT-PCR: occurrence of mixed P type infections.","authors":"J D Mascarenhas, F L Paiva, C R Barardi, Y B Gabbay, C O Simões, A C Linhares","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fifty-four group A rotavirus-positive stool samples, obtained from children aged less than three years during a longitudinal (December 1982 to March 1986) study in Belém, Brazil, were re-examined. The samples were tested by reverse-transcription and polymerase chain reaction to determine their G-type and P-type specificity. Only 17 (32%) of these rotavirus strains could be successfully G- and P-genotyped. While 10 (59%) of the 17 strains showed single G- and P-type specificity, the remaining belonged to single G- and mixed P-genotypes. Rotavirus strains P[8], G1 and P[4], G1 predominated, accounting for 29% and 18% of the typed strains respectively. Mixed P-type infections caused by rotaviruses classified as P[8] + P[4], G1 were identified in 23% of cases. All but 3 of the 54 rotavirus strains displayed long genomic profiles, as demonstrated by the analysis of RNA by polyacrylamide gel electrophoresis. Most (70%) rotavirus strains with single G- and P-type specificity were detected during the first year of life, whereas 5 (71%) of the seven mixed P-type infections occurred throughout the second or third year of age. Reinfections were noted in two children, both of them being infected with P[8] + P[4], G1 rotavirus strains when aged 20 months. The high proportion of untypeable rotavirus strains suggests that unusual types may be circulating in Belém. In addition, the occurrence of mixed P-type infections in our region indicates the potential for reassortment between different rotavirus genogroups. Monitoring of these rotavirus strains may have important implication in the context of future strategies of rotavirus vaccination in Brazil.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 1","pages":"8-14"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20688419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A simple method for measuring caeco-colonic transit time in mice.","authors":"S M Robert, J P Rao","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 1","pages":"15-7"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20688422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P K Ghosh, S Gupta, L R Leon, R Ghosh, B H Ruiz Ordaz, L Ortiz-Ortiz
{"title":"Intestinal amoebiasis: antibody-secreting cells and humoral antibodies.","authors":"P K Ghosh, S Gupta, L R Leon, R Ghosh, B H Ruiz Ordaz, L Ortiz-Ortiz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Splenic plasma cell response and systemic antibody response to intestinal amoebiasis were studied in C3H/HeJ mice from 5 to 60 days post-inoculation with Entamoeba histolytica. At various time intervals specific antibody-secreting cells (ASC) in the spleen were measured in infected mice and non-infected control mice by enzyme-linked immunospot (ELISPOT) assay. Serum antibodies were measured by enzyme-linked immunosorbent assay (ELISA). The infected animals showed high IgA ASC from 30 to 50 days post-inoculation as compared to IgM and IgG ASC. However, class-specific serum antibody showed high IgG titre from 30 to 60 days post-inoculation as compared to IgM and IgA serum titres. Our results suggest that E. histolytica trophozoites can induce a plasma cell response in the spleen that is different from anti-amoebic antibody response in serum.</p>","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"16 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1998-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20688418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mechanism of purgative effect of magnesium sulphate on mouse colon.","authors":"F S Robert, J P Rao","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77203,"journal":{"name":"Journal of diarrhoeal diseases research","volume":"15 4","pages":"248-9"},"PeriodicalIF":0.0,"publicationDate":"1997-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20578668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}