Journal of cellular biochemistry. Supplement最新文献_第7页

Linkages of nuclear architecture to biological and pathological control of gene expression. 核结构与基因表达的生物和病理控制的联系。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01 DOI: 10.1002/(sici)1097-4644(1998)72:30/31+<220::aid-jcb27>3.3.co;2-n

G. Stein, A. V. van Wijnen, J. Stein, J. Lian, S. Pockwinse, S. McNeil

{"title":"Linkages of nuclear architecture to biological and pathological control of gene expression.","authors":"G. Stein, A. V. van Wijnen, J. Stein, J. Lian, S. Pockwinse, S. McNeil","doi":"10.1002/(sici)1097-4644(1998)72:30/31+<220::aid-jcb27>3.3.co;2-n","DOIUrl":"https://doi.org/10.1002/(sici)1097-4644(1998)72:30/31+<220::aid-jcb27>3.3.co;2-n","url":null,"abstract":"Functional interrelationships between components of nuclear architecture and control of gene expression are becoming increasingly evident. There is growing appreciation that multiple levels of nuclear organization integrate the regulatory cues that support activation and suppression of genes as well as the processing of gene transcripts. The linear organization of genes and promoter elements provide the potential for responsiveness to physiological regulatory signals. Parameters of chromatin structure and nucleosome organization support synergism between activities at independent regulatory sequences and render promoter elements accessible or refractory to transcription factors. Association of genes, transcription factors, and the machinery for transcript processing with the nuclear matrix facilitates fidelity of gene expression within the three-dimensional context of nuclear architecture. Mechanisms must be defined that couple nuclear morphology with enzymatic parameters of gene expression. The recent characterization of factors that mediate chromatin remodeling and intranuclear targeting signals that direct transcription factors to subnuclear domains where gene expression occurs, reflect linkage of genetic and structural components of transcriptional control. Nuclear reorganization and aberrant intranuclear trafficking of transcription factors for developmental and tissue-specific control that occurs in tumor cells and in neurological disorders provides a basis for high resolution diagnostic and targeted therapy.","PeriodicalId":77196,"journal":{"name":"Journal of cellular biochemistry. Supplement","volume":" 13","pages":"220-31"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50637163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In search of cellular control: signal transduction in context. 在寻找细胞控制:信号转导的背景。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01 DOI: 10.1002/(sici)1097-4644(1998)72:30/31+<232::aid-jcb28>3.3.co;2-f

D. Ingber

{"title":"In search of cellular control: signal transduction in context.","authors":"D. Ingber","doi":"10.1002/(sici)1097-4644(1998)72:30/31+<232::aid-jcb28>3.3.co;2-f","DOIUrl":"https://doi.org/10.1002/(sici)1097-4644(1998)72:30/31+<232::aid-jcb28>3.3.co;2-f","url":null,"abstract":"The field of molecular cell biology has experienced enormous advances over the last century by reducing the complexity of living cells into simpler molecular components and binding interactions that are amenable to rigorous biochemical analysis. However, as our tools become more powerful, there is a tendency to define mechanisms by what we can measure. The field is currently dominated by efforts to identify the key molecules and sequences that mediate the function of critical receptors, signal transducers, and molecular switches. Unfortunately, these conventional experimental approaches ignore the importance of supramolecular control mechanisms that play a critical role in cellular regulation. Thus, the significance of individual molecular constituents cannot be fully understood when studied in isolation because their function may vary depending on their context within the structural complexity of the living cell. These higher-order regulatory mechanisms are based on the cell's use of a form of solid-state biochemistry in which molecular components that mediate biochemical processing and signal transduction are immobilized on insoluble cytoskeletal scaffolds in the cytoplasm and nucleus. Key to the understanding of this form of cellular regulation is the realization that chemistry is structure and hence, recognition of the the importance of architecture and mechanics for signal integration and biochemical control. Recent work that has unified chemical and mechanical signaling pathways provides a glimpse of how this form of higher-order cellular control may function and where paths may lie in the future.","PeriodicalId":77196,"journal":{"name":"Journal of cellular biochemistry. Supplement","volume":" 3","pages":"232-7"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50637173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marrow stromal cells as stem cells for continual renewal of nonhematopoietic tissues and as potential vectors for gene therapy. 骨髓基质细胞作为非造血组织持续更新的干细胞和基因治疗的潜在载体。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01 DOI: 10.1002/(sici)1097-4644(1998)72:30/31+<284::aid-jcb34>3.3.co;2-9

D. Prockop

引用次数: 0

Cyclin-dependent kinase inhibitors in restriction point control, genomic stability, and tumorigenesis. 细胞周期蛋白依赖性激酶抑制剂在限制点控制、基因组稳定性和肿瘤发生中的作用。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01 DOI: 10.1002/(SICI)1097-4644(1998)72:30/31+<37::AID-JCB6>3.0.CO;2-W

S. Millard, A. Koff

引用次数: 11

Tissue engineering: the first decade and beyond. 组织工程:第一个十年及以后。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01

L J Bonassar, C A Vacanti

引用次数: 0

Bone stem cells. 骨干细胞。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01

J E Aubin

引用次数: 0

Promiscuous subunit interactions: a possible mechanism for the regulation of protein kinase CK2. 混杂亚基相互作用:蛋白激酶CK2调控的可能机制。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01

C C Allende, J E Allende

{"title":"Promiscuous subunit interactions: a possible mechanism for the regulation of protein kinase CK2.","authors":"C C Allende, J E Allende","doi":"","DOIUrl":"","url":null,"abstract":"Protein kinase CK2 is a ubiquitous eukaryotic ser/thr protein kinase. The active holoenzyme is a heterotetrameric protein composed of catalytic (alpha and alpha') and regulatory (beta) subunits that phosphorylates many different protein substrates and appears to be involved in the regulation of cell division. Despite important structural studies, the intimate details of the interactions of the alpha catalytic subunits with the beta regulatory subunits are unknown. Recent evidence that indicates that both CK2 subunits can interact promiscuously with other proteins in a manner that excludes the binding of their complementary CK2 partners has opened the possibility that the phosphorylating activity of this enzyme may be regulated in a novel way. These alternative interactions could limit the in vivo availability of CK2 subunits to generate fully active holoenzyme CK2 tetramers. Likewise, variations in the ratio of alpha- and beta-subunits could determine the activity of several phosphorylating and dephosphorylating activities. The promiscuity of the CK2 subunits can be extrapolated to a more widespread phenomenon in which \"wild-card\" proteins could act as general switches by interacting and regulating several catalytic activities.","PeriodicalId":77196,"journal":{"name":"Journal of cellular biochemistry. Supplement","volume":"30-31 ","pages":"129-36"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20800118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A role for cadherins in cellular signaling and differentiation. 钙粘蛋白在细胞信号传导和分化中的作用。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01

K A Knudsen, C Frankowski, K R Johnson, M J Wheelock

引用次数: 0

Steroid receptors at the nexus of transcriptional regulation. 类固醇受体在转录调控的关系。

Journal of cellular biochemistry. Supplement Pub Date : 1998-01-01 DOI: 10.1002/(sici)1097-4644(1998)72:30/31+<185::aid-jcb23>3.3.co;2-d

T. J. Barrett, T. Spelsberg

{"title":"Steroid receptors at the nexus of transcriptional regulation.","authors":"T. J. Barrett, T. Spelsberg","doi":"10.1002/(sici)1097-4644(1998)72:30/31+<185::aid-jcb23>3.3.co;2-d","DOIUrl":"https://doi.org/10.1002/(sici)1097-4644(1998)72:30/31+<185::aid-jcb23>3.3.co;2-d","url":null,"abstract":"During the past few years, our understanding of nuclear receptor action has dramatically improved as a result of the identification and functional analysis of co-regulators such as factors involved in chromatin remodeling, transcription intermediary factors (co-repressors and co-activators), and direct interactions with the basal transcriptional machinery. Furthermore, the elucidation of the crystal structures of the empty ligand-binding domains of the nuclear receptor and of complexes formed by the nuclear receptor's ligand-binding domain bound to agonists and antagonists has contributed significantly to our understanding of the early events of nuclear receptor action. However, the picture of hormone- and hormone receptor-mediated mechanisms of gene regulation remain incomplete and extremely complicated when one also considers the \"nontraditional\" interactions of hormone-activated nuclear receptors, for example, interactions between the activated steroid receptors and components of the chromatin/nuclear matrix; and finally the nongenomic effects that steroid hormones can exhibit with other signaling pathways. In this prospectus on steroid receptors, we discuss the implications of various steroid hormone and nuclear receptor interactions and potential future directions of investigation.","PeriodicalId":77196,"journal":{"name":"Journal of cellular biochemistry. Supplement","volume":" 12","pages":"185-93"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50637105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of human prostate cancer models for chemoprevention and experimental therapeutics studies. 用于化学预防和实验治疗的人类前列腺癌模型的建立。

Journal of cellular biochemistry. Supplement Pub Date : 1997-01-01

L W Chung, H E Zhau, T T Wu

{"title":"Development of human prostate cancer models for chemoprevention and experimental therapeutics studies.","authors":"L W Chung, H E Zhau, T T Wu","doi":"","DOIUrl":"","url":null,"abstract":"The progression of human prostate cancer from histomorphologic to clinical expression often requires several decades. This study emphasizes the importance of developing relevant human prostate cancer models to study the molecular events leading to prostate cancer progression. These models will provide a rational basis for chemopreventive and treatment strategies to retard the progression of human prostate cancer from its localized to its metastatic state. In our laboratory, we have established the LNCaP progression and ARCaP models and the in vitro three-dimensional growth models involving prostate cancer and bone stroma to study the progression of prostate cancer. We propose that prostate cancer may progress from an androgen-dependent to an androgen-independent state. While existing as androgen-independent tumors (defined as tumors capable of growing in castrated hosts and secreting PSA in serum), prostate cancer may assume three different phenotypes as it progresses: androgen-independent while remaining androgen-responsive; androgen-independent and unresponsive to androgen stimulation; and androgen-independent but suppressed by androgen. It is conceivable that any androgen-independent human prostate cancer may contain variable proportions of cells that exhibit these three phenotypes. This concept may have important implications in determining strategies for chemopreventive and therapeutic trials. We have established three-dimensional growth models of prostate cancer cells either in collagen gel or microgravity-simulated growth conditions to form viable and functional organoids which contain prostate cancer epithelial cells admixed with prostate or bone stromal cells. These in vitro models combined with the in vivo models described above will enhance our understanding of the regulatory mechanism of prostate cancer growth and progression, and hence could improve efficiency in screening chemopreventive and therapeutic agents which alter the biologic behaviors of human prostate cancer.","PeriodicalId":77196,"journal":{"name":"Journal of cellular biochemistry. Supplement","volume":"28-29 ","pages":"174-81"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20510086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0