Journal of cell science. Supplement最新文献_第5页

Defective acidification of the biosynthetic pathway in cystic fibrosis. 囊性纤维化中生物合成途径的酸化缺陷。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.32

J Barasch, Q al-Awqati

引用次数: 81

The cytoskeleton in development of epithelial cell polarity. 上皮细胞极性发育过程中的细胞骨架。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.10

K R Fath, S N Mamajiwalla, D R Burgess

{"title":"The cytoskeleton in development of epithelial cell polarity.","authors":"K R Fath, S N Mamajiwalla, D R Burgess","doi":"10.1242/jcs.1993.supplement_17.10","DOIUrl":"https://doi.org/10.1242/jcs.1993.supplement_17.10","url":null,"abstract":"The polarization of intestinal epithelial cells and the stereotypic arrangement of their actin-based cytoskeleton have made these epithelia an excellent system to explore the organization and formation of a cortical actin-based cytoskeleton. Through a combined morphological and biochemical analysis, the molecular arrangement of many of the components of the brush border has been elucidated. Study of brush border assembly in the Crypts of Lieberkühn suggests that cytoskeletal mRNA and protein expression, as well as morphological development, occur rapidly following cell differentiation. Protein kinases appear to be important regulators of intestinal cell growth, for differentiating cells in the crypts possess 15-fold higher levels of tyrosine phosphorylated proteins than differentiated cells of the villus. One of these kinases, pp60c-src, has a 4- to 7-fold higher activity in crypts and increased association with the cytoskeleton than it has in villus cells. The development and maintenance of polarization in epithelial cells require the targeting and transport of specific proteins to the apical and basolateral plasma membrane. It has been proposed that a dynein-like, microtubule-based motor is involved in the transport of apically directed materials from the trans-Golgi to the apical plasma membrane. However, microtubules do not reach the plasma membrane, but terminate below the actin-rich network of filaments comprising the terminal web. We propose that vesicles translocate from the Golgi to the apical cytoplasm along microtubules using dynein, and then move through the terminal web to reach the apical plasma membrane using the actin-based motor myosin-I.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77195,"journal":{"name":"Journal of cell science. Supplement","volume":"17 ","pages":"65-73"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1242/jcs.1993.supplement_17.10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18519394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 77

The role of transepithelial transport by M cells in microbial invasion and host defense. M细胞经上皮转运在微生物入侵和宿主防御中的作用。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.29

M R Neutra, J P Kraehenbuhl

引用次数: 24

Molecular sorting in polarized and non-polarized cells: common problems, common solutions. 极化和非极化细胞的分子分选:常见问题，常见解决方案。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.1

I Mellman, E Yamamoto, J A Whitney, M Kim, W Hunziker, K Matter

{"title":"Molecular sorting in polarized and non-polarized cells: common problems, common solutions.","authors":"I Mellman, E Yamamoto, J A Whitney, M Kim, W Hunziker, K Matter","doi":"10.1242/jcs.1993.supplement_17.1","DOIUrl":"https://doi.org/10.1242/jcs.1993.supplement_17.1","url":null,"abstract":"We have been interested in determining how epithelial cells generate and maintain their characteristically polarized distributions of membrane proteins. Our efforts to date strongly indicate that the polarized transport in MDCK cells may be due to a set of discrete targeting determinants often found on a membrane protein's cytoplasmic domain. Surprisingly, these determinants are widely distributed and are not specific to proteins expressed in polarized cells. They also appear to function in controlling polarized transport along both the biosynthetic and the endocytic (or transcytotic) pathways. Signals for basolateral transport have been characterized and, like the cytoplasmic domain signal used by plasma membrane receptors for accumulation at clathrin-coated pits, they often involve a critical tyrosine residue. Although the basolateral and coated pit signals may also be co-linear, they are not identical. The basolateral and apical transport determinants are also hierarchically arranged. Although a single protein may contain one or more signals specifying basolateral transport, inactivation of these signals appears to reveal a determinant that directs efficient apical transport. Given that the sequence determinants responsible for polarized transport are not restricted to epithelial cells and are related to determinants commonly utilized in all cells, it is possible that non-polarized cells contain cognate apical and basolateral pathways that are responsible for 'constitutive' transport from the Golgi to the plasma membrane. The presence of two cognate pathways might confer a high degree of plasticity to pre-differentiated cells, allowing them rapidly to begin assuming a polarized phenotype in response to extracellular stimuli without requiring the synthesis of epithelial cell-specific transport machinery.","PeriodicalId":77195,"journal":{"name":"Journal of cell science. Supplement","volume":"17 ","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1242/jcs.1993.supplement_17.1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19136216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Ankyrin-binding activity of nervous system cell adhesion molecules expressed in adult brain. 成人脑神经系统细胞黏附分子表达的锚蛋白结合活性。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.16

J Q Davis, V Bennett

{"title":"Ankyrin-binding activity of nervous system cell adhesion molecules expressed in adult brain.","authors":"J Q Davis, V Bennett","doi":"10.1242/jcs.1993.supplement_17.16","DOIUrl":"https://doi.org/10.1242/jcs.1993.supplement_17.16","url":null,"abstract":"A family of ankyrin-binding glycoproteins have been identified in adult rat brain that include alternatively spliced products of the same pre-mRNA. A composite sequence of ankyrin-binding glycoprotein (ABGP) shares 72% amino acid sequence identity with chicken neurofascin, a membrane-spanning neural cell adhesion molecule in the Ig super-family expressed in embryonic brain. ABGP polypeptides and ankyrin associate as pure proteins in a 1:1 molar stoichiometry at a site located in the predicted cytoplasmic domain. ABGP polypeptides are expressed late in postnatal development to approximately the same levels as ankyrin, and comprise a significant fraction of brain membrane proteins. Immunofluorescence studies have shown that ABGP polypeptides are co-localized with ankyrinB. Major differences in developmental expression have been reported for neurofascin in embryos compared with the late postnatal expression of ABGP, suggesting that ABGP and neurofascin represent products of gene duplication events that have subsequently evolved in parallel with distinct roles. Predicted cytoplasmic domains of rat ABGP and chicken neurofascin are nearly identical to each other and closely related to a group of nervous system cell adhesion molecules with variable extracellular domains, including L1, Nr-CAM and Ng-CAM of vertebrates, and neuroglian of Drosophila. A hypothesis to be evaluated is that ankyrin-binding activity is shared by all of these proteins.","PeriodicalId":77195,"journal":{"name":"Journal of cell science. Supplement","volume":"17 ","pages":"109-17"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1242/jcs.1993.supplement_17.16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19136218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Responses to Wnt signals in vertebrate embryos may involve changes in cell adhesion and cell movement. 脊椎动物胚胎对Wnt信号的反应可能涉及细胞粘附和细胞运动的改变。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.26

R T Moon, A DeMarais, D J Olson

{"title":"Responses to Wnt signals in vertebrate embryos may involve changes in cell adhesion and cell movement.","authors":"R T Moon, A DeMarais, D J Olson","doi":"10.1242/jcs.1993.supplement_17.26","DOIUrl":"https://doi.org/10.1242/jcs.1993.supplement_17.26","url":null,"abstract":"Wnt genes encode secreted glycoproteins, and, because of their homology with the Drosophila segment polarity gene wingless, are likely to play important roles as modulators of local intercellular signalling during embryonic development. Although little is known of the mechanisms by which Wnts signal in an autocrine or paracrine manner, it is increasingly clear that cells can respond rapidly to Wnt signals in the absence of transcription, and that these responses may include changes in cell adhesion and cell movement. We review recent evidence from studies on Xenopus laevis and other systems, which demonstrate that (1) a subset of Wnts modulate gap junctional permeability, which may be a reflection of changes in cadherin-mediated cell adhesion, (2) embryos express beta-catenin and plakoglobin, which are homologs of the armadillo gene products, known to be involved in the wingless signalling pathway, and known to be found at cell junctions, and (3) overexpression of specific Wnts in Xenopus embryos leads to clear changes in cell behavior and movement.","PeriodicalId":77195,"journal":{"name":"Journal of cell science. Supplement","volume":"17 ","pages":"183-8"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1242/jcs.1993.supplement_17.26","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19136710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 41

Biogenesis of structural intercellular junctions during cleavage in the mouse embryo. 小鼠胚胎卵裂过程中结构细胞间连接的生物发生。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.17

T P Fleming, Q Javed, J Collins, M Hay

{"title":"Biogenesis of structural intercellular junctions during cleavage in the mouse embryo.","authors":"T P Fleming, Q Javed, J Collins, M Hay","doi":"10.1242/jcs.1993.supplement_17.17","DOIUrl":"https://doi.org/10.1242/jcs.1993.supplement_17.17","url":null,"abstract":"The preimplantation embryo differentiates the trophectoderm epithelium which, from the 32-cell stage, generates the blastocoel of the blastocyst and, after implantation, gives rise to most extraembryonic lineages of the conceptus. Trophectoderm differentiation begins at compaction (8-cell stage) when cell-cell adhesion, mediated by uvomorulin, and epithelial cell polarisation first occur. Here, we review our work on the biogenesis of tight junctions and desmosomes during epithelial differentiation. Tight junction construction begins at compaction and appears to be a gradual process, both at morphological and molecular levels. This maturation pattern may be due in part to sequential expression of tight junction constituents from the embryonic genome. Tight junction formation is dependent upon uvomorulin adhesion but can be inhibited by different means without apparently disturbing cell adhesion or polarisation. Cell interactions appear to regulate tight junction tissue specificity, in part by controlling the level of synthesis of constituents. Desmosome formation begins at the 32-cell stage, particularly as the embryo initiates blastocoel accumulation, and, in contrast with tight junction formation, does not appear to be a gradual process. Thus, nascent desmosomes appear mature in terms of their molecular composition. Desmosomal proteins are synthesised well in advance of desmosome formation but the synthesis of the principal glycoprotein components begins at the blastocyst stage and may regulate the timing of junction assembly. Implications of these differing patterns of biogenesis for the embryo are discussed.","PeriodicalId":77195,"journal":{"name":"Journal of cell science. Supplement","volume":"17 ","pages":"119-25"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1242/jcs.1993.supplement_17.17","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19136219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Catenins as mediators of the cytoplasmic functions of cadherins. 钙粘蛋白作为钙粘蛋白细胞质功能的介质。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.22

B M Gumbiner, P D McCrea

引用次数: 257

Dysfunction of CFTR bearing the delta F508 mutation. 携带delta F508突变的CFTR功能障碍。

Journal of cell science. Supplement Pub Date : 1993-01-01

M J Welsh, G M Denning, L S Ostedgaard, M P Anderson

引用次数: 0

Polarity signals in epithelial cells. 上皮细胞中的极性信号。

Journal of cell science. Supplement Pub Date : 1993-01-01 DOI: 10.1242/jcs.1993.supplement_17.2

E Rodriguez-Boulan, C Zurzolo

引用次数: 45