Aerobiologia最新文献

{"title":"Assessment of Serratia marcescens bioaerosol for the biological testing of biosafety cabinet","authors":"Ji-Yon Kye,&nbsp;Hyon-Hak Pak,&nbsp;Jong-Hyok Jon,&nbsp;Hyok-Chol Choe,&nbsp;Jong-Sim Ri,&nbsp;Song-Nam Hong,&nbsp;Su-Ryon Ryom","doi":"10.1007/s10453-025-09900-x","DOIUrl":"10.1007/s10453-025-09900-x","url":null,"abstract":"<div><p><i>Serratia marcescens</i> is widely used as a model strain in aerobiological researches for its apparent colony morphology and easy removablilty from the working environment. In contrast, <i>Bacillus subtilis</i> var. <i>niger</i> ATCC 9372 is highly resistant to environmental stresses, so requires a laboring decontamination process after test. To substitute for this traditional test strain in the biological testing of Class II Biosafety Cabinets (BSC), we evaluated the characteristics of <i>S. marcescens</i> bioaerosol including count median diameter (CMD), the ratio of single cells, and the total viable CFU (Colony forming unit) stability. When the suspension of the <i>S. marcescens</i> for bioaerosol production was prepared via shake culture, the CMD of <i>S. marcescens</i> bioaerosol was 1.72 μm, the ratio of single cells was 92.6%, in accordance with the NSF/ANSI 49 standards. However, the total viable CFU of the <i>S. marcescens</i> bioaerosol tended to decline when prolonged storage of the primary slant culture and the suspension. <i>S. marcescens</i> bioaerosol might be used as a test strain for the biological testing of BSC only when the suspension is prepared using shake culture with a predetermined storage duration of the primary slant culture and the suspension. The relative accuracy, relative specificity, and relative sensitivity of this alternative method using <i>S. marcescens</i> bioaerosol was 92.6%, 91.7%, and 93.3% in personnel protection, 92.6%, 87.5%, and 94.7% in product protection of the biological testing of BSC when compared with the standard method using <i>B. subtilis</i> var. <i>niger</i>.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does a long-term pollen monitoring assess the risk of increasing local exposure to common ragweed (Ambrosia artemisiifolia) allergens?","authors":"Artur Górecki,&nbsp;Artur Pliszko,&nbsp;Katarzyna Piotrowicz,&nbsp;Monika Ziemianin,&nbsp;Dorota Myszkowska","doi":"10.1007/s10453-025-09899-1","DOIUrl":"10.1007/s10453-025-09899-1","url":null,"abstract":"<div><p><i>Ambrosia artemisiifolia</i> (common ragweed), an annual plant native to North America, is an invasive species in Europe, including Poland, posing economic issues and significant health risks due to its highly allergenic pollen. In Poland, common ragweed is still relatively sparsely distributed, but the number of persistent populations has recently increased. This study aimed to check whether an invasion of common ragweed observed since 2020 in Kraków (southern Poland) affected the pollen seasons in the following years. The aerobiological study has been supported by an ongoing botanical survey during which 38 new sites have been described. The local invasion of <i>A</i>. <i>artemisiifolia</i> populations has noticeably affected the recorded pollen seasons since 2020, increasing the risk of exposure for residents, mainly due to the proximity of residential and recreational areas to the largest populations of common ragweed. However, trend analysis did not show the significant long-term tendencies for the most pollen season parameters; SPIn value and the number of high-pollen days increased evidently within the study period. Local ragweed invasion is becoming an increasingly important source of pollen, although pollen exposure from the long-distance transport (LDT) episodes in southern and eastern regions continues to be a large source of pollen.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A spatial explicit method combining HYSPLIT and random forest for predicting the next-day pollen index at urban scale","authors":"Zihang Huang,&nbsp;Teng Fei,&nbsp;Meng Bian","doi":"10.1007/s10453-025-09896-4","DOIUrl":"10.1007/s10453-025-09896-4","url":null,"abstract":"<div><p>Airborne pollen is a significant cause of allergies leading to various discomforting symptoms. Many cities worldwide, including Beijing, face airborne pollen challenges. Accurate forecasting of airborne pollen levels can provide critical risk alerts for residents. However, existing studies at urban scale often overlook the spatial variability of pollen levels, focusing predominantly on pollen from individual plant sources or using station-based forecasts, which limits their applicability. To address these shortcomings with available data, this study integrates the Hybrid Single-Particle Lagrangian Integrated Trajectory model with Random Forest to develop a novel method for predicting the spatial distribution of next-day pollen indexes derived from dominant tree species. Using Beijing as a case study, the proposed method demonstrates promising prediction accuracy, with R² of 0.67 for the city-averaged index. An analysis of pollen index distributions during Beijing's spring pollen season reveals that urban areas consistently exhibit higher predicted pollen indexes for the included tree species compared to the citywide average throughout the season. This trend suggests an elevated allergy risk in urban regions from these specific pollen types, likely due to pollen accumulation in semi-enclosed terrains influencing pollen transport and deposition. Furthermore, the study highlights that eliminating the included dominant tree pollen sources in city centers is insufficient to achieve significant reductions in the predicted urban pollen index. This research presents a new approach for generating spatially resolved pollen index forecasts, offering enhanced spatial detail compared to traditional station-based method, and enhanced spatial detail based on available operational data and dominant vegetation surveys, offering valuable insights for allergy prevention strategies.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10453-025-09896-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145982875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct sampling and bioanalyses of atmospheric bioaerosols via an unmanned aerial vehicle (UAV) over S17 Base, East Antarctica","authors":"Fumihisa Kobayashi","doi":"10.1007/s10453-025-09897-3","DOIUrl":"10.1007/s10453-025-09897-3","url":null,"abstract":"<div><p>Atmospheric bioaerosol sampling is being extensively conducted in Antarctica. In this study, bioaerosol sampling was conducted at a 900-m altitude over S17 Base in the coastal region of Antarctica using an unmanned aerial vehicle (UAV) on January 10, 2019. Based on the meteorological data collected using the UAV, bioaerosol samples were obtained from a high mixed layer and/or the low free troposphere. Bacteria belonging to the class Chloroplast were detected in the upper air. Those were also observed at an altitude of about 1000 m over Syowa Station in 2013 (Kobayashi, 2022a), suggesting that they may be persistent in the upper atmosphere over East Antarctica. In the upper air, bacteria belonging to the genera <i>Arcobacter</i>, <i>Finegoldia</i>, and <i>Methyloversatilis</i> were particularly detected. From backward trajectory analyses of the air mass, these bacteria may have been transported long distance. </p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145930620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Airborne dust particle-induced lung inflammation in mice alleviated by adipose stem cell-derived conditioned medium","authors":"Hazrat Zaman,&nbsp;Madiha Habib,&nbsp;Zobia Safdar,&nbsp;Shafi Zaman,&nbsp;Hafiza Sumbul Yousaf,&nbsp;Muhammad Shahbaz Aslam,&nbsp;Zaigham Abbas","doi":"10.1007/s10453-025-09894-6","DOIUrl":"10.1007/s10453-025-09894-6","url":null,"abstract":"<div><h3>Background</h3><p>Exposure to airborne dust particles increases the risks of respiratory illnesses, including asthma, chronic obstructive pulmonary disease, and bronchitis. Determining effective interventional strategies to mitigate toxicity induced by airborne dust particles may provide essential public health benefits. This study assessed the detrimental effects of airborne dust particles extracted from car air filters in Lahore City, alongside the therapeutic potential of adipose-derived stem cell conditioned medium (ADSC-CM) on cabin air filter (CAF) dust-induced lung damage in mice.</p><h3>Methods</h3><p>BALB/c mice were subjected to repeated intraperitoneal sensitization with CAF dust suspension followed by challenges with aerosolized dust in a nebulization chamber. Moreover, the treated group of mice received intravenous injections of ADSCs-CM before exposure to CAFs dust challenges. Subsequently, allergic manifestations, lung histopathological features, synthesis of inflammatory cytokines, and potential gene expressions linked to pulmonary diseases were investigated.</p><h3>Results</h3><p>CAF dust exposure triggered lung injury and inflammation characterized by epithelial sloughing, hyperplasia, pulmonary edema, and infiltration of inflammatory cells into the bronchi. Further studies showed an increased synthesis of inflammatory cytokines, notably IL-6 and TNF-α, and elevated expression of COX-2 and MUC5AC gene. Supplementation with ADSCs-CM substantially ameliorated lung inflammation, reduced allergic symptoms, induction of cytokines, COX-2, and MUC5AC genes expression.</p><h3>Conclusion</h3><p>CAF dust samples showed harmful effects on lung health, depicting potential risks to city residents. The results exhibited that ADSCs-CM transplantation substantially reduced lung inflammation and could serve as a promising treatment for pulmonary disorders.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145929838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability in the allergenic potential of Alternaria spores in the air of urban and rural environments","authors":"Jana Ščevková,&nbsp;Matúš Žilka,&nbsp;Zuzana Vašková,&nbsp;Jozef Dušička,&nbsp;Michal Hrabovský,&nbsp;Eva Zahradníková,&nbsp;Jozef Kováč","doi":"10.1007/s10453-025-09893-7","DOIUrl":"10.1007/s10453-025-09893-7","url":null,"abstract":"<div><p>Spores of the genus <i>Alternaria</i>, which are a common component of the atmospheric microbiome, are among the important fungal aeroallergens. The study aimed to (1) compare the characteristics of the <i>Alternaria</i> Main Spore Season (MSS) in an urban and rural area, (2) assess the correlation between the airborne concentration of <i>Alternaria</i> spores and Alt a 1 allergen, and (3) identify the environmental factors influencing the intensity of MSS and daily variation of airborne spore and Alt a 1 allergen levels. The airborne spores and allergen were recorded over two years (2022–2023) in the Kaplna village, and over three years (2021–2023) in Bratislava city (Slovakia), using Hirst-type samplers for spores and cyclone samplers for allergen. The intensity of the MSS varied significantly between individual years and locations, demonstrating the influence of environmental conditions. Higher values of the Seasonal Spore Integral (SSIn) were recorded in Kaplna, which has more plant biomass, but higher Spore Allergen Potency (SAP) levels were recorded in Bratislava, with a higher degree of urbanisation. In general, we found a positive correlation between the spore and Alt a 1 levels, but the SAP was negatively correlated with the concentration of spores. Meteorological parameters and air pollutants influenced the levels of spores, allergen, and SAP to a varying degree based on the individual year and location, with the most noticeable influence of relative humidity and precipitation decreasing the concentration of spores and increasing SAP.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dust mites from mattress dust: evaluating risk of mite allergy from guanine measurements","authors":"Indu Karuthedath,&nbsp;Sobha Thalakkattil Raghavan","doi":"10.1007/s10453-025-09892-8","DOIUrl":"10.1007/s10453-025-09892-8","url":null,"abstract":"<div><p>House dust mites have gained much attention recently because of their medical and veterinary significance. The house dust mite fauna and the allergies caused by these tiny mites remain relatively unexplored in Northern Kerala. The present study, conducted in two districts of Northern Kerala (viz., Malappuram and Kozhikode), aims to assess dust mite abundance in mattress dust from selected houses and to examine the dependence of various housing characteristics on mite populations. The housing variables considered included housing area, house roof type, number of residents, cleaning frequency, type of mattress, age of mattress and age of house. Out of 100 mattress dust samples collected, 90% of the samples were dust mite positive and <i>Dermatophagoides pteronyssinus</i> was found to be the most dominant (85.71%) mite species. A significant association was found between the number of mites collected and the number of residents. The estimation of guanine from dust samples gives an indirect measure of allergens present in the dust. Mattress dust from 50 houses was analysed for guanine content to assess the risk of mite allergy. All samples tested for guanine indicated low allergen levels (&lt; 0.6 mg/g dust), representing a potential risk for allergy development. However, guanine concentration measured across different mattress types showed no significant correlation with the mite density (Pearson correlation; <i>p</i> value: 0.606 &gt; 0.05).</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145778721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variations and spatial distribution of indoor airborne microorganisms in a university campus in Beijing: a high-throughput sequencing study of the culturable microbiome","authors":"Liu Yuheng,&nbsp;Deng Xunuo,&nbsp;Ma Shenglun,&nbsp;He Junde,&nbsp;Ma Xuezheng,&nbsp;Chen Xiaoqin","doi":"10.1007/s10453-025-09891-9","DOIUrl":"10.1007/s10453-025-09891-9","url":null,"abstract":"<div><p>The spatiotemporal distribution and variation of airborne microorganisms in densely populated university campus area are critical to the health of students and faculties. This study collected air samples via a six-stage Andersen sampler from different indoor environments within a selected university campus, and the cultured microorganisms were collected for high-throughput sequencing to identify the species and density of the microbial community. The results revealed the density of airborne microorganisms was various between 495.09 ± 258.27 and 598.18 ± 314.59 CFU/m³ in different indoor spaces. The density of polluted airborne microorganisms was significantly higher in the winter season (696.55 ± 306.36 CFU/m³) than that in autumn (410.20 ± 138.94 CFU/m³) (<i>P</i> &lt; 0.001). The detected microbial alpha diversity was also significantly higher in the winter season than autumn (F = 5.88, <i>P</i> &lt; 0.05). The winter seasonal difference was characterized by the dominance of micro- particles with a diameter range from 0.65–2.1 μm that can be deposited inside lung tissue. The composition of microorganisms contained more stress-resistant phylum Bacillota (52%) in air samples collected from winter. Principal Coordinate Analysis (PCoA) confirmed that seasonal variation was the primary driver of community structure (R² = 0.152, <i>P</i> = 0.003). These findings reveal that insufficient ventilation during the winter heating period promotes microbial accumulation and structural shifts in the indoor airborne microbiome. The higher density of contaminated microorganisms combined with a higher number of micro-particles in the air within a closed environment in winter are significant health risks for people living in a northern campus. This study provides a scientific basis for the better management of indoor air quality and health risk prevention strategies in educational settings.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"42 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145766319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recommended terminology for aerobiological studies: automatic and real-time monitoring methods","authors":"Fiona Tummon,&nbsp;Katharina Bastl,&nbsp;Maximilian Bastl,&nbsp;Paul J. Beggs,&nbsp;Jordina Belmonte,&nbsp;Nicolas Bruffaerts,&nbsp;Athanasios Charalampopoulos,&nbsp;Bernard Clot,&nbsp;Antonella Cristofori,&nbsp;Benoît Crouzy,&nbsp;Athanasios Damialis,&nbsp;Sophie Erb,&nbsp;Carmen Galan,&nbsp;Elias Graf,&nbsp;Jörg Haus,&nbsp;Kilian Koch,&nbsp;Gian Lieberherr,&nbsp;Maria Lbadaoui-Darvas,&nbsp;Marie-Pierre Meurville,&nbsp;Erny Niederberger,&nbsp;Jose Oteros,&nbsp;Rosa Pérez-Badia,&nbsp;Helena Ribeiro,&nbsp;Andreas Schwendimann,&nbsp;Branko Sikoparija,&nbsp;Carsten Skjoth,&nbsp;Matt Smith,&nbsp;Mikhail Sofiev,&nbsp;Olga Sozinova,&nbsp;Laura Sukiene,&nbsp;Konstantina Vasilatou,&nbsp;Willem W. Verstraeten,&nbsp;Yanick Zeder,&nbsp;Franziska Zemmer","doi":"10.1007/s10453-025-09879-5","DOIUrl":"10.1007/s10453-025-09879-5","url":null,"abstract":"","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"41 4","pages":"847 - 853"},"PeriodicalIF":2.1,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s10453-025-09879-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composition and spatiotemporal variations in the indoor airborne bacterial communities in two Chilean medical centers","authors":"Elizabeth Carrazana,&nbsp;Tay Ruiz,&nbsp;Marco A. Campos,&nbsp;Joaquín I. Rilling,&nbsp;So Fujiyoshi,&nbsp;Fumito Maruyama,&nbsp;Milko A. Jorquera","doi":"10.1007/s10453-025-09887-5","DOIUrl":"10.1007/s10453-025-09887-5","url":null,"abstract":"<div><p>Bioaerosols are highly relevant in medical centers (MCs), where factors such as seasonality, human occupancy (HO), and ventilation systems (VS) are proposed to influence the airborne bacterial communities and potential airborne human pathogenic bacteria (PAHPB). We investigated the compositions and spatiotemporal variations in the airborne bacterial communities, including those of PAHPB and antibiotic resistance genes (ARG), in two MCs in Chile during 2021 and 2022. 16S rRNA gene amplicon sequencing and qPCR revealed significant (<i>p</i> &lt; 0.05) variations in diversity, composition, and gene abundance across the studied years. Proteobacteria, Actinobacteria, and Firmicutes were dominant in bioaerosols, with gene abundances ranging from 10³ to 10⁷ copies m³ and were negatively correlated with diversity. The PAHPB and ARG genes were variable, with <i>Kleb</i>2 (<i>Klebsiella pneumoniae</i>) and <i>bla</i>TEM (β-lactam) being the most prevalent. Although HO and VS influenced the bacterial communities, no significant correlations (<i>p</i> &lt; 0.05) with bacterial diversity were found. Interestingly, 6 genera in the core microbiome were associated with humans, whereas 19 genera were associated with the environment, suggesting that outdoor air is a relevant factor influencing bacterial communities in MCs. We provide baseline data for research on bioaerosols in MCs, highlighting the complexity of airborne bacterial communities and suggesting some factors influencing their spatiotemporal variations.</p></div>","PeriodicalId":7718,"journal":{"name":"Aerobiologia","volume":"41 4","pages":"821 - 839"},"PeriodicalIF":2.1,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145547052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}