HPB surgery : a world journal of hepatic, pancreatic and biliary surgery最新文献_第7页

The Preoperative Assessment of Hepatic Tumours: Evaluation of UK Regional Multidisciplinary Team Performance. 肝肿瘤的术前评估:评估英国区域多学科团队的表现。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2013-01-01 Epub Date: 2013-08-22 DOI: 10.1155/2013/861681

M G Wiggans, S A Jackson, B M T Fox, J D Mitchell, S Aroori, M J Bowles, E M Armstrong, J F Shirley, D A Stell

{"title":"The Preoperative Assessment of Hepatic Tumours: Evaluation of UK Regional Multidisciplinary Team Performance.","authors":"M G Wiggans, S A Jackson, B M T Fox, J D Mitchell, S Aroori, M J Bowles, E M Armstrong, J F Shirley, D A Stell","doi":"10.1155/2013/861681","DOIUrl":"https://doi.org/10.1155/2013/861681","url":null,"abstract":"Introduction. In the UK, patients where liver resection is contemplated are discussed at hepatobiliary multidisciplinary team (MDT) meetings. The aim was to assess MDT performance by identification of patients where radiological and pathological diagnoses differed. Materials and Methods. A retrospective review of a prospectively maintained database of all cases undergoing liver resection from March 2006 to January 2012 was performed. The presumed diagnosis as a result of radiological investigation and MDT discussion is recorded at the time of surgery. Imaging was reviewed by specialist gastrointestinal radiologists, and resultswereagreedonby consensus. Results. Four hundred and thirty-eight patients were studied. There was a significant increase in the use of preoperative imaging modalities (P ≤ 0.01) but no change in the rate of discrepant diagnosis over time. Forty-two individuals were identified whose final histological diagnosis was different to that following MDT discussion (9.6%). These included 30% of patients diagnosed preoperatively with hepatocellular carcinoma and 25% with cholangiocarcinoma of a major duct. Discussion. MDT assessment of patients preoperatively is accurate in terms of diagnosis. The highest rate of discrepancies occurred in patients with focal lesions without chronic liver disease or primary cancer, where hepatocellular carcinoma was overdiagnosed and peripheral cholangiocarcinoma underdiagnosed, where particular care should be taken. Additional care should be taken in these groups and preoperative multimodality imaging considered. ","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2013 ","pages":"861681"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/861681","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31756541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Intraportal versus Systemic Pentoxifylline Infusion after Normothermic Liver Ischemia: Effects on Regional Blood Flow Redistribution and Hepatic Ischemia-Reperfusion Injury. 常温肝缺血后门静脉内输注与全身己酮可可碱:对局部血流再分布和肝缺血再灌注损伤的影响。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2013-01-01 Epub Date: 2013-08-29 DOI: 10.1155/2013/689835

Edson A Ribeiro, Luiz F Poli-de-Figueiredo, Rodrigo Vincenzi, Flavio H F Galvao, Nelson Margarido, Mauricio Rocha-E-Silva, Ruy J Cruz

{"title":"Intraportal versus Systemic Pentoxifylline Infusion after Normothermic Liver Ischemia: Effects on Regional Blood Flow Redistribution and Hepatic Ischemia-Reperfusion Injury.","authors":"Edson A Ribeiro, Luiz F Poli-de-Figueiredo, Rodrigo Vincenzi, Flavio H F Galvao, Nelson Margarido, Mauricio Rocha-E-Silva, Ruy J Cruz","doi":"10.1155/2013/689835","DOIUrl":"https://doi.org/10.1155/2013/689835","url":null,"abstract":"Pentoxifylline (PTX) has been shown to have beneficial effects on microcirculatory blood flow. In this study we evaluate the potential hemodynamic and metabolic benefits of PTX during hepatic ischemia. We also test the hypothesis that portal PTX infusion can minimize the I/R injury when compared to systemic infusion. Methods. Twenty-four dogs (18.1 ± 0.7 kg) were subjected to portal triad occlusion (PTO) for 45 min. The animals were assigned to 3 groups: CT (control, PTO, n = 8), PTX-syst (PTO + 25 mg/Kg of PTX IV, n = 8), and PTX-pv (PTO + 25 mg/Kg of PTX in the portal vein, n = 8). Animals were followed for 120 min. Systemic hemodynamics, gastrointestinal tract perfusion, oxygen-derived variables, and liver enzymes were evaluated throughout the experiment. Results. Animals treated with PTX presented significantly higher CO in the first hour after reperfusion, when compared to the CT (~3.7 vs. 2.1 L/min, P < 0.05). Alanine aminotransferase (ALT) was similar in the PTX groups two hours after reperfusion but significantly higher in the CT (227 vs. ~64 U/L, P < 0.05). Conclusion. PTX infusion was associated with hemodynamic benefits and was able to minimize liver injury during normothermic hepatic I/R. However, local PTX infusion was not associated with any significant advantage over systemic route. ","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2013 ","pages":"689835"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/689835","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31763407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Prognostic impact of preoperative imaging parameters on resectability of hilar cholangiocarcinoma. 术前影像学参数对肝门胆管癌可切除性的影响。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2013-01-01 Epub Date: 2013-06-04 DOI: 10.1155/2013/657309

Anthony T Ruys, Olivier R Busch, Erik A Rauws, Dirk J Gouma, Thomas M van Gulik

{"title":"Prognostic impact of preoperative imaging parameters on resectability of hilar cholangiocarcinoma.","authors":"Anthony T Ruys, Olivier R Busch, Erik A Rauws, Dirk J Gouma, Thomas M van Gulik","doi":"10.1155/2013/657309","DOIUrl":"https://doi.org/10.1155/2013/657309","url":null,"abstract":"Objectives. To evaluate, in hilar cholangiocarcinoma (HCCA), the prognostic impact of specific preoperative radiologic parameters on resectability, metastases, and yield of laparoscopy, and to evaluate the currently used staging systems. Methods. Consecutive patients with HCCA presenting in our center from January 2003 through August 2010 were evaluated. Suspicion on lymph node metastasis, portal vein and hepatic artery involvement, lobar atrophy, and proximal extent of ductal invasion was scored. The prognostic value of these parameters for predicting resectability, yield of diagnostic laparoscopy, likelihood of metastatic disease, R0 resection, and survival was assessed. The Bismuth-Corlette classification and MSKCC staging system were evaluated. Results. Of all 289 evaluated patients, 158 patients (55%) had unresectable disease based on cross-sectional imaging studies or diagnostic laparoscopy; 131 patients (45%) underwent exploration. 83 patients (64%) underwent resection, of whom 67 (87%) had a radical (R0) resection. Suspicious lymph nodes and involvement of the hepatic artery were important prognostic factors for resectability. Predictive power of the evaluated staging systems was limited. Conclusions. Current staging systems predict resectability, but there is room for improvement. Hepatic artery involvement and nodal status might be important factors for prediction of resectability and should be considered in future staging systems. ","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2013 ","pages":"657309"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/657309","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31586271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Renal dysfunction is an independent risk factor for mortality after liver resection and the main determinant of outcome in posthepatectomy liver failure. 肾功能不全是肝切除术后死亡的独立危险因素，也是肝切除术后肝衰竭预后的主要决定因素。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2013-01-01 Epub Date: 2013-11-05 DOI: 10.1155/2013/875367

M G Wiggans, G Shahtahmassebi, M J Bowles, S Aroori, D A Stell

{"title":"Renal dysfunction is an independent risk factor for mortality after liver resection and the main determinant of outcome in posthepatectomy liver failure.","authors":"M G Wiggans, G Shahtahmassebi, M J Bowles, S Aroori, D A Stell","doi":"10.1155/2013/875367","DOIUrl":"https://doi.org/10.1155/2013/875367","url":null,"abstract":"Introduction. The aim of this study was to assess the interaction of liver and renal dysfunction as risk factors for mortality after liver resection. Materials and Methods. A retrospective analysis of 501 patients undergoing liver resection in a single unit was undertaken. Posthepatectomy liver failure (PHLF) was defined according to the International Study Group of Liver Surgery (ISGLS) definition (assessed on day 5) and renal dysfunction according to RIFLE criteria. 90-day mortality was recorded. Results. Twenty-three patients died within 90 days of surgery (4.6%). The lowest mortality occurred in patients without evidence of PHLF or renal dysfunction (2.7%). The mortality rate in patients with isolated PHLF or renal dysfunction was 20% compared to 45% in patients with both. Diabetes (P = 0.028), renal dysfunction (P = 0.030), and PHLF on day 5 (P = 0.011) were independent predictors of 90-day mortality. Discussion. PHLF and postoperative renal dysfunction are independent predictors of 90-day mortality following liver resection but the predictive value for mortality is significantly higher when failure of both organ systems occurs simultaneously. ","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2013 ","pages":"875367"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/875367","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31920811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Surgical options for initially unresectable colorectal liver metastases. 最初不可切除的结直肠肝转移的手术选择。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2012-01-01 Epub Date: 2012-10-03 DOI: 10.1155/2012/454026

Irinel Popescu, Sorin Tiberiu Alexandrescu

{"title":"Surgical options for initially unresectable colorectal liver metastases.","authors":"Irinel Popescu, Sorin Tiberiu Alexandrescu","doi":"10.1155/2012/454026","DOIUrl":"https://doi.org/10.1155/2012/454026","url":null,"abstract":"Although the frontiers of liver resection for colorectal liver metastases have broadened in recent decades, approximately 75% of these patients present with unresectable metastases at the time of their diagnosis. In the past, these patients underwent only palliative treatment, without the chance of a cure. In the previous two decades, several therapeutic strategies have been developed that render resectable those metastases that were initially unresectable, thus offering the chance of long-term survival and even a cure to these patients. The oncosurgical modalities that are available include liver resection following portal vein ligation/embolization, \"two-stage\" liver resection, one-stage ultrasonically guided liver resection, hepatectomy following conversion chemotherapy, and liver resection combined with thermal ablation. Moreover, in recent years, certain authors have recommended the revisiting of the concept of liver transplantation in highly selected patients with unresectable colorectal liver metastases and favorable prognostic factors. By employing such therapies, the number of patients with colorectal liver metastases who undergo a potentially curative treatment could increase to 40%. The safety profile of these approaches is acceptable (morbidity rates as high as 45%, mortality rates of less than 5%). Furthermore, the 5-year survival rates (approximately 30%) are significantly increased over those that were achieved with palliative treatment.","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2012 ","pages":"454026"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/454026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30988673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Desferrioxamine Attenuates Pancreatic Injury after Major Hepatectomy under Vascular Control of the Liver: Experimental Study in Pigs. 去铁胺减轻肝脏血管控制下大肝切除术后胰腺损伤:猪的实验研究。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2012-01-01 Epub Date: 2012-06-25 DOI: 10.1155/2012/714672

Panagiotis Varsos, Constantinos Nastos, Nikolaos Papoutsidakis, Konstantinos Kalimeris, George Defterevos, Tzortzis Nomikos, Agathi Pafiti, George Fragulidis, Emmanuel Economou, Georgia Kostopanagiotou, Vassilios Smyrniotis, Nikolaos Arkadopoulos

{"title":"Desferrioxamine Attenuates Pancreatic Injury after Major Hepatectomy under Vascular Control of the Liver: Experimental Study in Pigs.","authors":"Panagiotis Varsos, Constantinos Nastos, Nikolaos Papoutsidakis, Konstantinos Kalimeris, George Defterevos, Tzortzis Nomikos, Agathi Pafiti, George Fragulidis, Emmanuel Economou, Georgia Kostopanagiotou, Vassilios Smyrniotis, Nikolaos Arkadopoulos","doi":"10.1155/2012/714672","DOIUrl":"https://doi.org/10.1155/2012/714672","url":null,"abstract":"Introduction. Pancreatic injury can manifest after major hepatectomy under vascular control. The main mechanism involved seems to be remote oxidative injury due to \"spillage\" of reactive oxygen species and cytokines from the liver. The aim of this study is to evaluate the role of desferrioxamine in the prevention of pancreatic injury following major hepatectomy. Methods. Twelve Landrace pigs were subjected to a combination of major hepatectomy (70-75%), using the Pringle maneuver for 150 minutes, after constructing a porta-caval side-to-side anastomosis. The duration of reperfusion was 24 hours. Animals were randomly divided into a control group (n = 6) and a desferrioxamine group (DFX, n = 6). DFX animals were treated with continuous IV infusion of desferrioxamine 100 mg/kg. Pancreatic tissue injury, c-peptide and amylase concentrations, and pancreatic tissue oxidative markers were evaluated. Results. Desferrioxamine-treated animals showed decreased c-peptide levels, decreased acinar cell necrosis, and decreased tissue malondialdehyde levels 24 hours after reperfusion compared with the control group. There was no difference in portal pressure or serum amylase levels between the groups. Conclusions. Desferrioxamine seems to attenuate pancreatic injury after major hepatectomy under vascular control possibly by preventing and reversing production and circulation of oxidative products.","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2012 ","pages":"714672"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/714672","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30759894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anesthetic Considerations in Hepatectomies under Hepatic Vascular Control. 肝血管控制下肝切除术的麻醉注意事项

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2012-01-01 Epub Date: 2012-05-28 DOI: 10.1155/2012/720754

Aliki Tympa, Kassiani Theodoraki, Athanassia Tsaroucha, Nikolaos Arkadopoulos, Ioannis Vassiliou, Vassilios Smyrniotis

{"title":"Anesthetic Considerations in Hepatectomies under Hepatic Vascular Control.","authors":"Aliki Tympa, Kassiani Theodoraki, Athanassia Tsaroucha, Nikolaos Arkadopoulos, Ioannis Vassiliou, Vassilios Smyrniotis","doi":"10.1155/2012/720754","DOIUrl":"10.1155/2012/720754","url":null,"abstract":"Background. Hazards of liver surgery have been attenuated by the evolution in methods of hepatic vascular control and the anesthetic management. In this paper, the anesthetic considerations during hepatic vascular occlusion techniques were reviewed. Methods. A Medline literature search using the terms \"anesthetic,\" \"anesthesia,\" \"liver,\" \"hepatectomy,\" \"inflow,\" \"outflow occlusion,\" \"Pringle,\" \"hemodynamic,\" \"air embolism,\" \"blood loss,\" \"transfusion,\" \"ischemia-reperfusion,\" \"preconditioning,\" was performed. Results. Task-orientated anesthetic management, according to the performed method of hepatic vascular occlusion, ameliorates the surgical outcome and improves the morbidity and mortality rates, following liver surgery. Conclusions. Hepatic vascular occlusion techniques share common anesthetic considerations in terms of preoperative assessment, monitoring, induction, and maintenance of anesthesia. On the other hand, the hemodynamic management, the prevention of vascular air embolism, blood transfusion, and liver injury are plausible when the anesthetic plan is scheduled according to the method of hepatic vascular occlusion performed.","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2012 ","pages":"720754"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3368350/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30683420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ischemia/Reperfusion injury in liver surgery and transplantation: pathophysiology. 肝手术和移植中的缺血/再灌注损伤:病理生理学。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2012-01-01 Epub Date: 2012-05-30 DOI: 10.1155/2012/176723

Kilian Weigand, Sylvia Brost, Niels Steinebrunner, Markus Büchler, Peter Schemmer, Martina Müller

{"title":"Ischemia/Reperfusion injury in liver surgery and transplantation: pathophysiology.","authors":"Kilian Weigand, Sylvia Brost, Niels Steinebrunner, Markus Büchler, Peter Schemmer, Martina Müller","doi":"10.1155/2012/176723","DOIUrl":"https://doi.org/10.1155/2012/176723","url":null,"abstract":"Liver ischemia/reperfusion (IR) injury is caused by a heavily toothed network of interactions of cells of the immune system, cytokine production, and reduced microcirculatory blood flow in the liver. These complex networks are further elaborated by multiple intracellular pathways activated by cytokines, chemokines, and danger-associated molecular patterns. Furthermore, intracellular ionic disturbances and especially mitochondrial disorders play an important role leading to apoptosis and necrosis of hepatocytes in IR injury. Overall, enhanced production of reactive oxygen species, found very early in IR injury, plays an important role in liver tissue damage at several points within these complex networks. Many contributors to IR injury are only incompletely understood so far. This paper tempts to give an overview of the different mechanisms involved in the formation of IR injury. Only by further elucidation of these complex mechanisms IR injury can be understood and possible therapeutic strategies can be improved or be developed.","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2012 ","pages":"176723"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/176723","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30686142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 121

Minocycline decreases liver injury after hemorrhagic shock and resuscitation in mice. 二甲胺四环素减轻小鼠失血性休克和复苏后的肝损伤。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2012-01-01 Epub Date: 2012-06-07 DOI: 10.1155/2012/259512

Christoph Czerny, Andaleb Kholmukhamedov, Tom P Theruvath, Eduardo N Maldonado, Venkat K Ramshesh, Mark Lehnert, Ingo Marzi, Zhi Zhong, John J Lemasters

{"title":"Minocycline decreases liver injury after hemorrhagic shock and resuscitation in mice.","authors":"Christoph Czerny, Andaleb Kholmukhamedov, Tom P Theruvath, Eduardo N Maldonado, Venkat K Ramshesh, Mark Lehnert, Ingo Marzi, Zhi Zhong, John J Lemasters","doi":"10.1155/2012/259512","DOIUrl":"https://doi.org/10.1155/2012/259512","url":null,"abstract":"Patients that survive hemorrhage and resuscitation (H/R) may develop a systemic inflammatory response syndrome (SIRS) that leads to dysfunction of vital organs (multiple organ dysfunction syndrome, MODS). SIRS and MODS may involve mitochondrial dysfunction. Under pentobarbital anesthesia, C57BL6 mice were hemorrhaged to 30 mm Hg for 3 h and then resuscitated with shed blood plus half the volume of lactated Ringer's solution containing minocycline, tetracycline (both 10 mg/kg body weight) or vehicle. Serum alanine aminotransferase (ALT), necrosis, apoptosis and oxidative stress were assessed 6 h after resuscitation. Mitochondrial polarization was assessed by intravital microscopy. After H/R with vehicle or tetracycline, ALT increased to 4538 U/L and 3999 U/L, respectively, which minocycline decreased to 1763 U/L (P < 0.01). Necrosis and TUNEL also decreased from 24.5% and 17.7 cells/field, respectively, after vehicle to 8.3% and 8.7 cells/field after minocycline. Tetracycline failed to decrease necrosis (23.3%) but decreased apoptosis to 9 cells/field (P < 0.05). Minocycline and tetracycline also decreased caspase-3 activity in liver homogenates. Minocycline but not tetracycline decreased lipid peroxidation after resuscitation by 70% (P < 0.05). Intravital microscopy showed that minocycline preserved mitochondrial polarization after H/R (P < 0.05). In conclusion, minocycline decreases liver injury and oxidative stress after H/R by preventing mitochondrial dysfunction.","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2012 ","pages":"259512"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/259512","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30708322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Effects of a preconditioning oral nutritional supplement on pig livers after warm ischemia. 预适应口服营养补充剂对猪肝脏热缺血的影响。

HPB surgery : a world journal of hepatic, pancreatic and biliary surgery Pub Date : 2012-01-01 Epub Date: 2012-06-25 DOI: 10.1155/2012/783479

Arash Nickkholgh, Zhanqing Li, Xue Yi, Elvira Mohr, Rui Liang, Saulius Mikalauskas, Marie-Luise Gross, Markus Zorn, Steffen Benzing, Heinz Schneider, Markus W Büchler, Peter Schemmer

{"title":"Effects of a preconditioning oral nutritional supplement on pig livers after warm ischemia.","authors":"Arash Nickkholgh, Zhanqing Li, Xue Yi, Elvira Mohr, Rui Liang, Saulius Mikalauskas, Marie-Luise Gross, Markus Zorn, Steffen Benzing, Heinz Schneider, Markus W Büchler, Peter Schemmer","doi":"10.1155/2012/783479","DOIUrl":"https://doi.org/10.1155/2012/783479","url":null,"abstract":"Background. Several approaches have been proposed to pharmacologically ameliorate hepatic ischemia/reperfusion injury (IRI). This study was designed to evaluate the effects of a preconditioning oral nutritional supplement (pONS) containing glutamine, antioxidants, and green tea extract on hepatic warm IRI in pigs. Methods. pONS (70 g per serving, Fresenius Kabi, Germany) was dissolved in 250 mL tap water and given to pigs 24, 12, and 2 hrs before warm ischemia of the liver. A fourth dose was given 3 hrs after reperfusion. Controls were given the same amount of cellulose with the same volume of water. Two hours after the third dose of pONS, both the portal vein and the hepatic artery were clamped for 40 min. 0.5, 3, 6, and 8 hrs after reperfusion, heart rate (HR), mean arterial pressure (MAP), central venous pressure (CVP), portal venous flow (PVF), hepatic arterial flow (HAF), bile flow, and transaminases were measured. Liver tissue was taken 8 hrs after reperfusion for histology and immunohistochemistry. Results. HR, MAP, CVP, HAF, and PVF were comparable between the two groups. pONS significantly increased bile flow 8 hrs after reperfusion. ALT and AST were significantly lower after pONS. Histology showed significantly more severe necrosis and neutrophil infiltration in controls. pONS significantly decreased the index of immunohistochemical expression for TNF-α, MPO, and cleaved caspase-3 (P < 0.001). Conclusion. Administration of pONS before and after tissue damage protects the liver from warm IRI via mechanisms including decreasing oxidative stress, lipid peroxidation, apoptosis, and necrosis.","PeriodicalId":77165,"journal":{"name":"HPB surgery : a world journal of hepatic, pancreatic and biliary surgery","volume":"2012 ","pages":"783479"},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/783479","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30759895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8