Hematologic pathology最新文献_第7页

Disseminated intravascular coagulation syndromes. 弥散性血管内凝血综合征。

Hematologic pathology Pub Date : 1992-01-01

R L Bick, W F Baker

引用次数: 0

Chromosome observations of acute promyelocytic leukemia treated with all-trans retinoic acid. 全反式维甲酸治疗急性早幼粒细胞白血病的染色体观察。

Hematologic pathology Pub Date : 1992-01-01

X Wu, W W Wang, Z L Zhou, G C Zhu, Z C Yang, J S Ying, Y Zhu

引用次数: 0

Reactivity of monoclonal antibody B-ly7 with a subset of activated T cells and T-cell lymphomas. 单克隆抗体B-ly7对活化T细胞亚群和T细胞淋巴瘤的反应性。

Hematologic pathology Pub Date : 1992-01-01

L Visser, L Dabbagh, S Poppema

引用次数: 0

Hydroxyurea and sickle cell disease. 羟基脲和镰状细胞病。

Hematologic pathology Pub Date : 1992-01-01

E P Orringer, J C Parker

引用次数: 0

Novel restriction fragment length polymorphisms in the cellular oncogene SEA. 细胞癌基因SEA中新的限制性片段长度多态性。

Hematologic pathology Pub Date : 1992-01-01

P R Koduru, J C Goh, J D Broome

{"title":"Novel restriction fragment length polymorphisms in the cellular oncogene SEA.","authors":"P R Koduru, J C Goh, J D Broome","doi":"","DOIUrl":"","url":null,"abstract":"The human homologue of the SEA oncogene has been mapped recently to chromosome band 11q13. While studying the possible involvement of this gene in the variant translocation t(9;22;11) (q34;q11;q13) in a case of chronic myelogenous leukemia, we identified novel polymorphisms for XbaI and SacI restriction enzyme sites in the SEA gene. Frequency of the polymorphic alleles was studied in 100 samples from healthy controls, 94 samples from patients with non-Hodgkin's lymphoma, 25 samples from patients with benign lymphadenopathy, and 38 samples from patients with chronic myelogenous leukemia. XbaI digestion showed a three-allele polymorphism with two frequent alleles A (8.0 kb) and B (9.2 kb) and a rare allele (5.8 kb). After SacI digestion the probe identified two primary genotypes. Genotype I showed two hybridizable DNA fragments, one each of 6.6 and 3.5 kb. In genotype II the 3.5 kb fragment was absent, instead two smaller fragments, one each of 1.9 kb and 1.6 kb were present. The 6.6 kb fragment (allele AA) had three polymorphic sites generating 6.2 kb fragment (allele BB), 7.4 kb fragment (allele CC), and 7.8 kb fragment (allele DD). Frequencies of the two genotypes and the four alleles followed Mendelian proportions in all the samples studied. Furthermore, this study shows the importance of restriction map analysis of DNA in the vicinity of the probe of an oncogene to distinguish natural polymorphisms from the disease-related rearrangements in the gene.","PeriodicalId":77160,"journal":{"name":"Hematologic pathology","volume":"6 2","pages":"71-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12524479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro study of erythropoiesis in patients with aplastic anemia and myelodysplastic syndromes: a possible tool for prospective determination of the clinical effectiveness of growth factors. 再生障碍性贫血和骨髓增生异常综合征患者红细胞生成的体外研究:一种前瞻性测定生长因子临床有效性的可能工具

Hematologic pathology Pub Date : 1992-01-01

A Aoki, A Shibata

{"title":"In vitro study of erythropoiesis in patients with aplastic anemia and myelodysplastic syndromes: a possible tool for prospective determination of the clinical effectiveness of growth factors.","authors":"A Aoki, A Shibata","doi":"","DOIUrl":"","url":null,"abstract":"Effects of recombinant human erythropoietin (rhEpo) and the combination of recombinant human interleukin-3 (rhIL-3) or recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) with rhEpo on erythroid colony formation were examined in vitro in 13 patients with aplastic anemia and 16 with myelodysplastic syndromes (MDS). The methylcellulose cultures of marrow cells from normals and the patients yielded no erythroid colonies in the absence of rhEpo. In normals, CFU-E and BFU-E colony formation was significantly increased by adding either rhIL-3 or rhGM-CSF with rhEpo, compared with rhEpo alone, and rhIL-3 was more potent than rhGM-CSF to form colony-forming units and burst-forming units of erythroid (CFU-E) (BFU-E) colonies. By adding rhIL-3 with rhEpo, CFU-E colony formation was increased in half of patients with RA, compared with rhEpo alone, and by rhGM-CSF, in one third. Approximately one third or one fourth of the patients with MDS showed increased BFU-E colonies when rhIL-3 or rhGM-CSF were added to rhEpo. Cultures containing rhIL-3 or rhGM-CSF with rhEpo yielded larger numbers of BFU-E colonies in half of the patients with nonsevere aplastic anemia than those containing rhEpo alone. These observations suggest that the combination of these growth factors, especially rhIL-3 with rhEpo, is applicable to the treatment of anemia in some patients with aplastic anemia and MDS.","PeriodicalId":77160,"journal":{"name":"Hematologic pathology","volume":"6 3","pages":"143-53"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12599828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary splenic presentation of plasma cell dyscrasia: report of two cases. 脾原发浆细胞增生2例报告。

Hematologic pathology Pub Date : 1992-01-01

H P Horny, J Saal, E Kaiserling

{"title":"Primary splenic presentation of plasma cell dyscrasia: report of two cases.","authors":"H P Horny, J Saal, E Kaiserling","doi":"","DOIUrl":"","url":null,"abstract":"Primary splenic presentation of plasma cell tumors is extremely rare. Recently we observed two female patients with primary (initially solitary) plasmacytoma of the spleen. While the pathoanatomical diagnosis of plasmacytoma could be established easily, the clinical picture in both cases was puzzling and allowed no definitive diagnosis to be made. One of the patients exhibited a long-standing monoclonal gammopathy. Repeated bone marrow examinations in both patients revealed slight increase in plasma cells (between 5 and 10% of all nucleated cells), but no infiltrates of multiple myeloma. The leading clinical feature in both cases was pronounced splenomegaly (780 g and 1600 g). Histologically both spleens exhibited marked infiltration by pleomorphic plasma cells, with monotypic expression of IgG kappa in one case and of the light chain lambda in the other. A broad panel of monoclonal antibodies detecting various hemopoietic and nonhemopoietic antigens was used to determine the immunophenotype of the neoplastic plasma cells, but in both cases they reacted only with a minority of the antibodies applied. The bone marrow in both cases remained free of tumorous infiltrates, but the disease progressed a few months after splenectomy with infiltration of the liver in one case and of lymph nodes in the other. To summarize, these two cases are definitely not multiple myelomas but could represent a distinct entity among the plasma cell dyscrasias for which the preliminary term \"disseminated plasmacytoma with primary splenic presentation\" is proposed.","PeriodicalId":77160,"journal":{"name":"Hematologic pathology","volume":"6 3","pages":"155-60"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12599829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of hemonectin in the cell adhesion mechanisms of bone marrow. 血凝素在骨髓细胞粘附机制中的作用。

Hematologic pathology Pub Date : 1992-01-01

A D Campbell

引用次数: 0

Specificity of heme for hemopoietic recovery from AZT toxicity. 血红素对AZT毒性后造血恢复的特异性。

Hematologic pathology Pub Date : 1992-01-01

G Loewy, P Cook, T Ahmed, J D Lutton, M Nishimura, A Distenfeld, R D Levere, N G Abraham

{"title":"Specificity of heme for hemopoietic recovery from AZT toxicity.","authors":"G Loewy, P Cook, T Ahmed, J D Lutton, M Nishimura, A Distenfeld, R D Levere, N G Abraham","doi":"","DOIUrl":"","url":null,"abstract":"The toxicity of azidothymidine (AZT) was studied on normal human bone marrow hemopoietic colony growth as determined by assays of CFU-E, BFU-E, and CFU-GM. The potential sparing effect of hemin and heme analogues on AZT-suppressed bone marrow was also investigated. AZT at a lower concentration (0.1 mumol/L) inhibited CFU-E by 68%, BFU-E by 84%, and CFU-GM by 59%. AZT at a higher concentration (1.0 mumol/L) inhibited CFU-E by 88%, BFU-E by 90%, and CFU-GM by 69%. Addition of hemin (10 mumol/L) to cultures containing AZT (0.1 mumol/L) increased CFU-E growth by 279%, BFU-E by 282%, and CFU-GM by 72%. A similar concentration of heme analogues did not have an enhancing effect; in contrast, zinc protoporphyrin (ZnPP) was inhibitory to bone marrow progenitors CFU-E, BFU-E, and CFU-GM. In addition, no enhancement of colony growth was obtained when progenitor cells were cultured in the presence of 10(-2)-10(-5) M iron. These results demonstrate that exogenous hemin has a specific beneficial effect on human bone marrow hematopoietic progenitor cells which is not seen with iron or other metalloporphyrins. Furthermore, this beneficial effect includes a reversal of the cytotoxic effect of AZT on bone marrow progenitors.","PeriodicalId":77160,"journal":{"name":"Hematologic pathology","volume":"6 2","pages":"79-85"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12774384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Patents, biotechnology, and hematologic pathology. 专利、生物技术和血液病理学。

Hematologic pathology Pub Date : 1992-01-01

D M Kettelberger, P Gambrell, G McClung

引用次数: 0