Growth regulation最新文献_第9页

Developmental regulation of growth hormone binding in the gilthead sea bream, Sparus aurata. 金头鲷生长激素结合的发育调控。

Growth regulation Pub Date : 1994-03-01

H Martí-Palanca, J Pérez-Sánchez

引用次数: 0

A mathematical model for appraisal of the impact of GH binding protein on GH receptor binding. 一个评估生长激素结合蛋白对生长激素受体结合影响的数学模型。

Growth regulation Pub Date : 1994-03-01

Z Hochberg, M B Youdim, T Amit

{"title":"A mathematical model for appraisal of the impact of GH binding protein on GH receptor binding.","authors":"Z Hochberg, M B Youdim, T Amit","doi":"","DOIUrl":"","url":null,"abstract":"Discrepancies between GH measurements and growth rate of children have complicated diagnosis in a variety of clinical conditions. The competition of GH-BP with the GH-receptor towards GH-receptor binding can have a role in these discrepancies. A mathematical model was developed for appraising the availability of GH for receptor binding from measurements of serum GH by RIA and serum GH binding protein (BP) by a binding assay. Eighteen patients with high GH-BP (obesity), normal GH-BP (normal control) or low GH-BP (children, anorexia nervosa or cirrhosis of the liver) were the subjects of this study. Sera of patients with high, normal or low GH-BP levels were analyzed for their competition with [125I]hGH binding to rabbit liver membranes. Serum GH was measured by a commercial polyclonal RIA. Serum GH-BP was measured by a binding assay with dextran-coated charcoal separation. Receptor availability for GH was assessed by displacing of [125I]hGH from rabbit liver membranes. The decline in receptor availability for each hGH value, caused by GH-BP competition with the receptor, was calculated by subtraction of the percent displacement in the absence of GH-BP from the percent displacement in the presence of a given GH-BP value. The results were analyzed statistically to give a series of polynomes. These enabled the calculation of an activity factor for serum RIA GH levels, that should predict the receptor availability of each GH level, according to the concomitant GH-BP level.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 1","pages":"8-13"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19182972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clonal analysis of rat tibia growth plate chondrocytes in suspension culture--differential effects of growth hormone and insulin-like growth factor I. 大鼠悬浮培养胫骨生长板软骨细胞克隆分析——生长激素和胰岛素样生长因子I的差异作用。

Growth regulation Pub Date : 1994-03-01

C Ohlsson, O Isaksson, A Lindahl

{"title":"Clonal analysis of rat tibia growth plate chondrocytes in suspension culture--differential effects of growth hormone and insulin-like growth factor I.","authors":"C Ohlsson, O Isaksson, A Lindahl","doi":"","DOIUrl":"","url":null,"abstract":"The number of growth hormone (GH) receptors in cultured rat epiphyseal chondrocytes are increased with numbers of cell divisions in monolayer. We wanted to study if increased number of cell divisions in monolayer influence GH or insulin-like growth factor I (IGF-I) response in a subsequent suspension culture. Primary isolated chondrocytes from rat tibia growth plates were cultured in monolayer at different seeding densities (4000, 8000 and 24,000 cells/cm2). After a culture period of 7 days, cells were trypsinized, counted and subcultured at 50,000 cells per dish in suspension stabilized with 0.5% agarose. 14 days later the agarose cultures were dried, stained and the number of clones with a diameter exceeding 50 microns was counted. Individual clones were classified as undifferentiated or differentiated according to the following criteria: cell clusters with a diameter of 50 microns and without matrix staining were classified as undifferentiated; cell clusters with a diameter over 50 microns consisting of 4 cells or more and with matrix stained by Alcian Blue were classified as differentiated clones. Human growth hormone (hGH) added to the suspension culture medium increased the number of undifferentiated clones if cells had been precultured at 4000 and 8000 cells/cm2 but hGH had no stimulatory effect on either clone type at 24,000 cells/cm2. IGF-I significantly increased the number of differentiated clones at all seeding densities while no effect was demonstrated on the number of undifferentiated clones. The results from the present study suggest that an increased number of cell divisions during primary monolayer culture increases GH responsiveness in a subsequent suspension culture.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19185674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of body mass index on growth hormone secretion in normal and short statured children. 体重指数对正常和矮小儿童生长激素分泌的影响。

Growth regulation Pub Date : 1994-03-01

L Patel, A M Skinner, D A Price, P E Clayton

{"title":"The influence of body mass index on growth hormone secretion in normal and short statured children.","authors":"L Patel, A M Skinner, D A Price, P E Clayton","doi":"","DOIUrl":"","url":null,"abstract":"Obesity is one of the factors which limits the value of growth hormone (GH) provocation tests in the diagnosis of GH deficiency. We have therefore examined (1) the relationship between urinary GH (uGH), a physiological parameter of GH secretion, and percent ideal body mass index (BMI%), an indirect estimate of body fat, in 528 schoolchildren; and (2) the extent to which peak arginine stimulated (0.5 g/kg i.v.) GH concentrations were influenced by BMI% in 176 short normal (SN) children and 48 girls with Turner syndrome (TS). The mean BMI% (SD) for each group was 102.9 (10.8) in schoolboys, 102.7 (13.4) in schoolgirls, 95.8 (13.9) in SN boys, 98.2 (21.4) in SN girls and 105.9 (18.0) in TS. BMI% correlated inversely with log uGH in school-children (boys r = -0.16, P = 0.01; girls r = -0.25, P < 0.001). However, if each sex was subdivided by pubertal status, the inverse relationship only persisted in pubertal (boys r = -0.18, P = 0.04; girls r = -0.39, P < 0.001) but not prepubertal children (boys r = -0.1, P = 0.3; girls r = -0.11, P = 0.3). BMI% was also inversely related to log peak stimulated GH concentration in SN girls (r = -0.49, P < 0.001) but not SN boys (r = -0.14, P = 0.2) or girls with TS (r = 0.19, P = 0.2). The inverse relationship between normal body fat and physiological GH secretion becomes significant during puberty; in girls it accounts for 15% of the variability in uGH excretion.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19182971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin-like growth factors I and II are reduced in plasma from growth retarded children with chronic liver disease. 慢性肝病生长迟缓儿童血浆中胰岛素样生长因子I和II降低。

Growth regulation Pub Date : 1994-03-01

P Quirk, P Owens, K Moyse, S Chin, C Wall, J Ballard, R Shepherd

引用次数: 0

Involvement of G proteins in the effect of insulin-like growth factor I on gonadotropin-induced rat granulosa cell differentiation. G蛋白参与胰岛素样生长因子I对促性腺激素诱导的大鼠颗粒细胞分化的影响。

Growth regulation Pub Date : 1994-03-01

H He, A C Herington, P Roupas

{"title":"Involvement of G proteins in the effect of insulin-like growth factor I on gonadotropin-induced rat granulosa cell differentiation.","authors":"H He, A C Herington, P Roupas","doi":"","DOIUrl":"","url":null,"abstract":"Insulin-like growth factor I (IGF-I) promotes gonadotropin-induced granulosa cell differentiation and proliferation. In order to investigate whether guanine nucleotide binding proteins (G proteins) may be linked, directly or indirectly, to some of the actions of IGF, the effects of cholera toxin (CT) and pertussis toxin (PT) on the enhancement by IGF-I of PMSG (pregnant mare serum gonadotropin)-induced rat granulosa cell differentiation have been studied. This was done by the determination of progesterone production, aromatase activity and cAMP accumulation after a 48 h incubation with PMSG, IGF-I and PMSG plus IGF-I in cells treated with either CT or PT. Both CT and PT treatment stimulated PMSG-induced progesterone production in granulosa cells after 48 h of culture with PMSG. CT treatment also stimulated aromatase activity in cells treated with PMSG and increased cAMP secretion under basal conditions (untreated cells) and in PMSG treated cells. Both CT and PT increased the stimulation by IGF-I of PMSG-induced progesterone production after 48 h of culture with PMSG plus IGF-I. Furthermore, CT augmented the enhancement by IGF-I of PMSG-induced aromatase activity and cAMP accumulation. In the absence of PMSG, CT did not increase steroidogenesis either alone or in the presence of IGF-I within the time frame studied even though CT was able to stimulate cAMP accumulation in untreated and IGF-I treated cells. These results suggest that G proteins have a role in the signalling cascade involved in gonadotropin-induced granulosa cell differentiation measured as PMSG-mediated steroidogenesis.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 1","pages":"20-8"},"PeriodicalIF":0.0,"publicationDate":"1994-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19185676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin-like growth factor binding proteins: a review of methodological aspects of their purification, analysis and regulation. 胰岛素样生长因子结合蛋白:纯化、分析和调控的方法学方面综述。

Growth regulation Pub Date : 1994-02-01

J M Holly, J L Martin

引用次数: 0

Transcriptional and post-transcriptional regulation of protein synthesis. 蛋白质合成的转录和转录后调控。

Growth regulation Pub Date : 1994-02-01

P K Lund

引用次数: 0

3rd International Symposium on Insulin-like Growth Factors. Sydney, Australia, February 6-10, 1994. Papers and abstracts. 第三届胰岛素样生长因子国际研讨会。1994年2月6日至10日，澳大利亚悉尼。论文和摘要。

Growth regulation Pub Date : 1994-02-01

引用次数: 0