Growth regulation最新文献_第8页

Effects of recombinant bovine somatotropin implants on serum concentrations of somatotropin, insulin-like growth factor-I and blood urea nitrogen in steers. 重组牛生长激素植入物对牛血清生长激素、胰岛素样生长因子- 1和血尿素氮浓度的影响。

Growth regulation Pub Date : 1994-09-01

R A Roeder, M J Garber, B S Dalke, T R Kasser, J Veenhuizen, G T Schelling

{"title":"Effects of recombinant bovine somatotropin implants on serum concentrations of somatotropin, insulin-like growth factor-I and blood urea nitrogen in steers.","authors":"R A Roeder, M J Garber, B S Dalke, T R Kasser, J Veenhuizen, G T Schelling","doi":"","DOIUrl":"","url":null,"abstract":"Four cross-bred beef steers averaging 346 kg were used in a 4 x 4 Latin square design to determine the effect of prolonged-release recombinant bovine somatotropin (rbGH) implants on serum concentrations of somatotropin (GH), insulin-like growth factor-I (IGF-I) and blood urea nitrogen (BUN). Recombinant bGH implants of 0, 40, 80 or 160 mg were administered subcutaneously in the tailhead during the 4 trial periods. Each steer received each treatment starting at 06:00 on day 0 with 21 days between treatments. Jugular vein blood samples were collected on days 0, 1, 2 and 3 (4 day time course for GH, IGF-I and BUN) and every 15 min (GH profile) for 6 h on day 3. Serum baseline GH values were higher (P < 0.10) for the 80 and 160 mg treatments than for the control, and peak amplitude was decreased (P < 0.05) by the 40 and 160 mg treatments. There was a trend (P < 0.11) for fewer GH peaks during the 160 mg treatment. Somatotropin concentrations decreased from day 1 to day 3 (P < 0.05) in a linear manner. Serum IGF-I concentrations increased (P < 0.05) in a linear dose-dependent manner from the 0 mg to the 160 mg treatment. BUN concentrations were not significantly altered by rbGH treatment. Results from this experiment indicate that rbGH implants significantly increase serum IGF-I and GH baseline concentrations while suppressing GH peak amplitude in finishing steers.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 3","pages":"101-7"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18856182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth hormone stimulates proliferation of normal human bone marrow stromal osteoblast precursor cells in vitro. 生长激素刺激正常人骨髓基质成骨前体细胞体外增殖。

Growth regulation Pub Date : 1994-09-01

M Kassem, L Mosekilde, E F Eriksen

引用次数: 0

Insulin-like growth factors and their binding proteins in normal and abnormal human fetal growth. 胰岛素样生长因子及其结合蛋白在正常和异常人胎儿生长中的作用。

Growth regulation Pub Date : 1994-09-01

T Chard

{"title":"Insulin-like growth factors and their binding proteins in normal and abnormal human fetal growth.","authors":"T Chard","doi":"","DOIUrl":"","url":null,"abstract":"There is now a well recognized series of findings which suggests that the insulin-like growth factors (IGFs) and their binding proteins (IGFBPs) may play an important role in both normal and abnormal human fetal growth: (1) IGFs are detectable in many fetal tissues from the first trimester onwards; (2) the levels of the IGFs in the fetal circulation increase during pregnancy, and at term the levels of IGF-I are directly related to birthweight; (3) in mice, disruption of the IGF gene leads to severe growth retardation; (4) in the first trimester the levels of IGFBP-1 are higher in the coelomic fluid than in amniotic fluid or maternal serum; (5) at 9-12 weeks there is a striking increase in IGFBP-1 and IGFBP-2 levels in amniotic fluid; (6) the major binding proteins in the human fetus are IGFBP-1 and IGFBP-2; (7) from as early as 16 weeks there is an inverse correlation between fetal levels of IGFBP-1 and birthweight; (8) in the mother, circulating levels of IGF-I and IGFBP-1 increase during pregnancy; (10) maternal levels of IGFBP-1 are elevated in severe pre-eclampsia and intrauterine growth retardation; (11) fetal levels of IGFBP-1 are elevated in cases of intrauterine growth retardation, especially those associated with specific evidence of reduced uteroplacental bloodflow; and (12) fetal levels of IGFBP-1 are elevated in labour, especially if there is evidence of fetal hypoxia. In conclusion, levels of IGFBP-1 appear to be a sensitive indicator of fetal nutrition, and of the short- or long-term response to reduced fetal nutrition.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 3","pages":"91-100"},"PeriodicalIF":0.0,"publicationDate":"1994-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18540581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased ternary complex formation and predominance of a 29 kDa IGFBP-3 fragment in human fetal serum. 减少三元复合物的形成和29 kDa IGFBP-3片段在人胎儿血清中的优势。

Growth regulation Pub Date : 1994-06-01

P Bang, M Stangenberg, M Westgren, R G Rosenfeld

{"title":"Decreased ternary complex formation and predominance of a 29 kDa IGFBP-3 fragment in human fetal serum.","authors":"P Bang, M Stangenberg, M Westgren, R G Rosenfeld","doi":"","DOIUrl":"","url":null,"abstract":"Insulin-like growth factor-I (IGF-I) has been proposed to be important in the endocrine control of fetal growth in humans, although serum IGF-I concentrations are 10-fold lower than during rapid pubertal growth. However, the bioavailability of IGF-I in fetal serum may be increased by changes in the specific IGF binding proteins (IGFBPs). We have recently suggested that the bioavailability of circulating IGF-I is increased in the human fetus due to the molar excess of IGF-I plus IGF-II relative to IGFBP-3 as well as the increased concentrations of IGFBP-2, which does not form a long-lived ternary complex. We have presently studied ternary complex formation between IGF, IGFBP-3, and acid labile subunit (ALS) to further assess if IGF-I bioavailability is increased in human fetal serum. In 19-35 week gestation fetal sera, a markedly decreased formation of the ternary complex was demonstrated by the general absence of IGFBP-3 (detected by Western immunoblotting) in the approximately 130-150 kDa ternary complex after neutral size chromatography. The predominant form of IGFBP-3 in fetal serum was a 29 kDa fragment, which, following deglycosylation by Endoglycosidase-F, was demonstrated to consist of a approximately 20 kDa protein core. Despite the predominance of the 29 kDa IGFBP-3 fragment, we have previously demonstrated that the IGFBP-3 protease activity is not increased in fetal serum, in contrast to pregnancy or non-insulin dependent diabetes mellitus (NIDDM) sera.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 2","pages":"68-76"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18532437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of GRF with or without a SRIF antiserum on GH, IGF-1, thyroxin, cholecystokinin, gastrin and metabolite concentrations in growing rats. GRF加或不加SRIF抗血清对生长大鼠生长激素、IGF-1、甲状腺素、胆囊收缩素、胃泌素和代谢物浓度的影响

Growth regulation Pub Date : 1994-06-01

P Dubreuil, P Brazeau, J Morisset

{"title":"Effects of GRF with or without a SRIF antiserum on GH, IGF-1, thyroxin, cholecystokinin, gastrin and metabolite concentrations in growing rats.","authors":"P Dubreuil, P Brazeau, J Morisset","doi":"","DOIUrl":"","url":null,"abstract":"This study was undertaken to examine the effects of increasing doses of rat somatocrinin (GRF) with or without a somatostatin antiserum (SS-ab) on serum hormone and metabolic concentrations, as well as serum and duodenal cholecystokinin (CCK) and antral gastrin concentrations. 24-day-old male Sprague-Dawley rats were injected twice daily s.c. (10:00 and 16:30) for 14 consecutive days with either saline or rat GRF (1-43) NH2 (4 and 20 micrograms/kg) in gelatin. Three other groups of animals received the same treatment in association with the SS-ab given i.p. every other day making up the 6 groups of 12 animals in a 2 x 3 factorial design experiment. GRF treatment increased circulating growth hormone (GH) concentrations in a dose-dependent manner, alone or in combination with the SS-ab; the SS-ab treatment alone or combined with GRF also increased GH concentrations. Total hypophyseal GH content was increased (P < 0.05) by the GRF treatment alone. Serum levels of IGF-1, acetoacetate, alpha 2 globulin and antral gastrin were all increased by the GRF treatment with plateaus observed for antral gastrin and serum IGF-1 levels at the intermediary dose of GRF. Serum concentrations of T4 were reduced at the 4 micrograms/kg dose of GRF. Serum concentrations of CCK were increased by the SS-ab treatment alone, an effect reversed by increasing doses of GRF. Rat GRF produced a dose-dependent increase and decrease of alpha 2 globulin and albumin, respectively. These data indicate that GRF, probably via its effect on GH release, influences gastrointestinal hormone levels which are implicated in gastrointestinal organ growth and digestive processes.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 2","pages":"56-62"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18946976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impaired growth hormone secretion in patients operated for pituitary adenomas. 垂体腺瘤手术患者生长激素分泌受损。

Growth regulation Pub Date : 1994-06-01

H C Hoeck, F Bang, P Laurberg

引用次数: 0

Growth hormone and insulin-like growth factor I in athletes performing a marathon at 4000 m of altitude. 海拔4000米马拉松运动员的生长激素和胰岛素样生长因子I。

Growth regulation Pub Date : 1994-06-01

G Banfi, M Marinelli, G S Roi, A Colombini, M Pontillo, M Giacometti, S Wade

{"title":"Growth hormone and insulin-like growth factor I in athletes performing a marathon at 4000 m of altitude.","authors":"G Banfi, M Marinelli, G S Roi, A Colombini, M Pontillo, M Giacometti, S Wade","doi":"","DOIUrl":"","url":null,"abstract":"Human growth hormone (hGH) characteristically increases during physical exercise. In sports medicine, hGH changes have mostly been described in short-term performances under standardized laboratory procedures. We studied hGH and insulin-like growth factor I (IGF-I) under field conditions in 7 runners performing a marathon at 4000 m of altitude, to study the hormonal changes under extreme endurance effort and to evaluate the release of 22 kDa and 20 kDa monomers under these conditions, in comparison with a control group. The blood samples were taken at sea level, after 1 week of acclimatization, immediately after the run, and after a recovery of 24 h from the marathon. hGH was quantified by using a polyclonal method, a 22 kDa specific monoclonal method and a monoclonal method recognizing both 22 kDa and 20 kDa isoforms. A significant increase in hGH was noted using all methods after the marathon, followed by a significant decrease after recovery. Significant differences between athletes and controls were found only for the measurement after the marathon. No statistically reliable decrease of IGF-I was observed in athletes and controls. We obtained more limited hGH rises than previously described in athletes; the importance of lactate levels indicates that the use of physical exercise as a stimulation test for hGH should be closely standardized. The 20 kDa monomer and other hGH molecular forms played a limited role in the hormone increase: in our experience, elevation of hGH in heavy exercise corresponds mostly to the 22 kDa isoform. We suggest that the evaluation of hGH elevation in sports medicine could be accurately performed using specific 22 kDa monoclonal assays.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 2","pages":"82-6"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18946879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevention of hematopoietic myeloid and megakaryocyte toxicity associated with zidovudine in vivo in mice with recombinant GM-CSF. 重组GM-CSF在小鼠体内预防齐多夫定相关的造血髓细胞和巨核细胞毒性。

Growth regulation Pub Date : 1994-06-01

V S Gallicchio, N K Hughes, K F Tse

{"title":"Prevention of hematopoietic myeloid and megakaryocyte toxicity associated with zidovudine in vivo in mice with recombinant GM-CSF.","authors":"V S Gallicchio, N K Hughes, K F Tse","doi":"","DOIUrl":"","url":null,"abstract":"We studied the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the suppression of hematopoiesis associated with the use of the antiviral drug zidovudine (AZT) administered in vivo to normal mice, as determined by measuring peripheral blood indices, and assays of hematopoietic progenitors, i.e. erythroid (CFU-E/BFU-E), myeloid (CFU-GM), and megakaryocyte (CFU-Meg) from bone marrow and spleen. Previous studies from this laboratory have established that dose-escalation zidovudine induced a dose-dependent decrease in hematocrit, WBC, and platelets with altered populations of bone marrow and splenic erythroid, myeloid and megakaryocyte progenitors when administered to normal mice. Daily administration of GM-CSF (10 micrograms/kg/bw) was associated with altered peripheral blood indices and progenitor cells. Dose-escalation AZT, i.e. 0.1, 1.0 and 2.5 mg/ml, was associated with a comparable reduction in all indices, i.e. hematocrit, WBC, and platelets during the 6-week examination period. GM-CSF reduced zidovudine-induced myeloid toxicity (concentration < 2.5 mg/ml) which was associated with an increase in bone marrow and splenic CFU-GM. High concentration, i.e. 2.5 mg/ml still produced myelosuppression irreversible with GM-CSF. GM-CSF induced a reduction in circulating platelets following zidovudine treatment at weeks 2 and 4 with the 1.0 mg/ml and 2.5 mg/ml treatment groups respectively, compared to a persistent decrease in platelets in the presence of zidovudine alone. GM-CSF BFU-E were elevated indicating the restriction in erythoid differentiation was still present. These studies demonstrate GM-CSF influences myeloid and megakaryocyte recovery, but not the erythoid suppression associated with the antiviral drug zidovudine.","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 2","pages":"41-7"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18946975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell- and stage-specific expression of basic FGF in the developing rat gonads. 基础FGF在发育中的大鼠性腺的细胞特异性和分期特异性表达。

Growth regulation Pub Date : 1994-06-01

S Koike, T Noumura

引用次数: 0

Protease-induced alteration of insulin-like growth factor binding protein-3 as detected by radioimmunoassay. Agreement with ligand blotting data. 蛋白酶诱导的胰岛素样生长因子结合蛋白-3的改变与配体印迹数据一致。

Growth regulation Pub Date : 1994-06-01

C Lassarre, C Lalou, L Perin, M Binoux

{"title":"Protease-induced alteration of insulin-like growth factor binding protein-3 as detected by radioimmunoassay. Agreement with ligand blotting data.","authors":"C Lassarre, C Lalou, L Perin, M Binoux","doi":"","DOIUrl":"","url":null,"abstract":"Structural alteration of insulin-like growth factor binding protein-3 (IGFBP-3) resulting from limited proteolysis by one or more serine proteases in vivo was first described in the serum of pregnant women and in certain pathological conditions. Western immunoblotting has since been employed to detect the phenomenon in normal serum, using a polyclonal antibody raised against recombinant human IGFBP-3 and a highly sensitive technique of visualization by chemiluminescence. The major proteolytic fragment of 30 kDa, which fails to be detected in native serum by ligand blotting owing to its weak affinity for IGFs, has proved clearly visible in all serum samples tested, sometimes accompanied by smaller fragments of 20 and 16 kDa. Among the serum samples analysed, increasing proportions of proteolysed IGFBP-3 were found in the following order: acromegalic patients, normal subjects, GH-deficient patients, pregnant women. In RIAs done with the same antibody, many of the serum samples yielded dose-response curves which were not parallel with standard curves, with lower gradients. In the samples where measurements were possible, apparent IGFBP-3 levels proved lower in pregnant women (2.28 +/- 0.23 mg/l, mean +/- SEM) than in normal adults (4.26 +/- 0.33 mg/l, P < 0.001). These observations, which contradict earlier reports of higher levels in pregnant women, suggest that the 30 kDa proteolytic fragment has a weaker affinity for the antibody than the intact IGFBP-3 (which in ligand- and immunoblotting appears as a characteristic 42-39 kDa doublet and which is barely or not detectable in pregnancy serum).(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":77148,"journal":{"name":"Growth regulation","volume":"4 2","pages":"48-55"},"PeriodicalIF":0.0,"publicationDate":"1994-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18532436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0