Genetic engineering最新文献

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Delitto perfetto targeted mutagenesis in yeast with oligonucleotides. 酵母寡核苷酸靶向诱变研究。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_9
F. Storici, M. Resnick
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引用次数: 58
Genotyping by mass spectrometry. 质谱法基因分型。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_1
Molly S Bray, Peter A Doris
{"title":"Genotyping by mass spectrometry.","authors":"Molly S Bray, Peter A Doris","doi":"10.1007/978-1-4615-0073-5_1","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Applications of plant antiviral proteins. 植物抗病毒蛋白的应用。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_7
Melan Wang, Katalin A Hudak
{"title":"Applications of plant antiviral proteins.","authors":"Melan Wang, Katalin A Hudak","doi":"10.1007/978-1-4615-0073-5_7","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_7","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"143-61"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Practical applications of rolling circle amplification of DNA templates. 滚圈扩增DNA模板的实际应用。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_3
Paul M Richardson, Chris Detter, Barry Schweitzer, Paul F Predki
{"title":"Practical applications of rolling circle amplification of DNA templates.","authors":"Paul M Richardson,&nbsp;Chris Detter,&nbsp;Barry Schweitzer,&nbsp;Paul F Predki","doi":"10.1007/978-1-4615-0073-5_3","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_3","url":null,"abstract":"<p><p>Since its recent implementation at one of the world's largest high-throughput sequencing centers, the utility of MP-RCA for DNA sequencing has been thoroughly validated. However, applications of this technology extend far beyond DNA sequencing. While many of these applications have been explored in this chapter, the future will undoubtedly add to this growing list.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"51-63"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Foreign DNA: integration and expression in transgenic plants. 外源DNA:在转基因植物中的整合与表达。
Genetic engineering Pub Date : 2002-01-01 DOI: 10.1007/978-1-4615-0721-5_6
Richard M Twyman, Ajay Kohli, Eva Stoger, Paul Christou
{"title":"Foreign DNA: integration and expression in transgenic plants.","authors":"Richard M Twyman,&nbsp;Ajay Kohli,&nbsp;Eva Stoger,&nbsp;Paul Christou","doi":"10.1007/978-1-4615-0721-5_6","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_6","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"107-36"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 28
Regulation of the Ras-MAPK pathway at the level of Ras and Raf. 在Ras和Raf水平上调控Ras- mapk通路。
Genetic engineering Pub Date : 2002-01-01 DOI: 10.1007/978-1-4615-0721-5_3
Haris Vikis, Kun-Liang Guan
{"title":"Regulation of the Ras-MAPK pathway at the level of Ras and Raf.","authors":"Haris Vikis,&nbsp;Kun-Liang Guan","doi":"10.1007/978-1-4615-0721-5_3","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_3","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"49-66"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Integrins and the myocardium. 整合素和心肌。
Genetic engineering Pub Date : 2002-01-01 DOI: 10.1007/978-1-4615-0721-5_5
Shaw-Yung Shai, Alice E Harpf, Robert S Ross
{"title":"Integrins and the myocardium.","authors":"Shaw-Yung Shai,&nbsp;Alice E Harpf,&nbsp;Robert S Ross","doi":"10.1007/978-1-4615-0721-5_5","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_5","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"87-105"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0721-5_5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Application of FLP/FRT site-specific DNA recombination system in plants. FLP/FRT位点特异性DNA重组系统在植物中的应用。
Genetic engineering Pub Date : 2002-01-01 DOI: 10.1007/978-1-4615-0721-5_1
Hong Luo, Albert P Kausch
{"title":"Application of FLP/FRT site-specific DNA recombination system in plants.","authors":"Hong Luo,&nbsp;Albert P Kausch","doi":"10.1007/978-1-4615-0721-5_1","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 20
Novel approaches to controlling transcription. 控制转录的新方法。
Genetic engineering Pub Date : 2002-01-01 DOI: 10.1007/978-1-4615-0721-5_7
Thomas D Schaal, Michael C Holmes, Edward J Rebar, Casey C Case
{"title":"Novel approaches to controlling transcription.","authors":"Thomas D Schaal,&nbsp;Michael C Holmes,&nbsp;Edward J Rebar,&nbsp;Casey C Case","doi":"10.1007/978-1-4615-0721-5_7","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_7","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"137-78"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Import of nuclear encoded RNAs into yeast and human mitochondria: experimental approaches and possible biomedical applications. 核编码rna导入酵母和人类线粒体:实验方法和可能的生物医学应用。
Genetic engineering Pub Date : 2002-01-01 DOI: 10.1007/978-1-4615-0721-5_9
N Entelis, O Kolesnikova, H Kazakova, I Brandina, P Kamenski, R P Martin, I Tarassov
{"title":"Import of nuclear encoded RNAs into yeast and human mitochondria: experimental approaches and possible biomedical applications.","authors":"N Entelis,&nbsp;O Kolesnikova,&nbsp;H Kazakova,&nbsp;I Brandina,&nbsp;P Kamenski,&nbsp;R P Martin,&nbsp;I Tarassov","doi":"10.1007/978-1-4615-0721-5_9","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_9","url":null,"abstract":"<p><p>Mitochondria import from the cytoplasm the vast majority of proteins and some RNAs. Although there exists extended knowledge concerning the mechanisms of protein import, the import of RNA is poorly understood. It was almost exclusively studied on the model of tRNA import, in several protozoans, plants and yeast. Mammalian mitochondria, which do not import tRNAs naturally, are hypothesized to import other small RNA molecules from the cytoplasm. We studied tRNA import in the yeast system, both in vitro and in vivo, and applied similar approaches to study 5S rRNA import into human mitochondria. Despite the obvious divergence of RNA import systems suggested for different species, we find that in yeast and human cells this pathway involves similar mechanisms exploiting cytosolic proteins to target the RNA to the organelle and requiring the integrity of pre-protein import apparatus. The import pathway might be of interest from a biomedical point of view, to target into mitochondria RNAs that could suppress pathological mutations in mitochondrial DNA. Yeast represents a good model to elaborate such a gene therapy approach. We have described here the various approaches and protocols to study RNA import into mitochondria of yeast and human cells in vitro and in vivo.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"191-213"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0721-5_9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
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