{"title":"Delitto perfetto targeted mutagenesis in yeast with oligonucleotides.","authors":"F. Storici, M. Resnick","doi":"10.1007/978-1-4615-0073-5_9","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_9","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"110 10","pages":"189-207"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50980343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genotyping by mass spectrometry.","authors":"Molly S Bray, Peter A Doris","doi":"10.1007/978-1-4615-0073-5_1","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"1-14"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paul M Richardson, Chris Detter, Barry Schweitzer, Paul F Predki
{"title":"Practical applications of rolling circle amplification of DNA templates.","authors":"Paul M Richardson, Chris Detter, Barry Schweitzer, Paul F Predki","doi":"10.1007/978-1-4615-0073-5_3","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_3","url":null,"abstract":"<p><p>Since its recent implementation at one of the world's largest high-throughput sequencing centers, the utility of MP-RCA for DNA sequencing has been thoroughly validated. However, applications of this technology extend far beyond DNA sequencing. While many of these applications have been explored in this chapter, the future will undoubtedly add to this growing list.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"51-63"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Regulation of the Ras-MAPK pathway at the level of Ras and Raf.","authors":"Haris Vikis, Kun-Liang Guan","doi":"10.1007/978-1-4615-0721-5_3","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_3","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"49-66"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Integrins and the myocardium.","authors":"Shaw-Yung Shai, Alice E Harpf, Robert S Ross","doi":"10.1007/978-1-4615-0721-5_5","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_5","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"87-105"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0721-5_5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of FLP/FRT site-specific DNA recombination system in plants.","authors":"Hong Luo, Albert P Kausch","doi":"10.1007/978-1-4615-0721-5_1","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"1-16"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thomas D Schaal, Michael C Holmes, Edward J Rebar, Casey C Case
{"title":"Novel approaches to controlling transcription.","authors":"Thomas D Schaal, Michael C Holmes, Edward J Rebar, Casey C Case","doi":"10.1007/978-1-4615-0721-5_7","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_7","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"137-78"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N Entelis, O Kolesnikova, H Kazakova, I Brandina, P Kamenski, R P Martin, I Tarassov
{"title":"Import of nuclear encoded RNAs into yeast and human mitochondria: experimental approaches and possible biomedical applications.","authors":"N Entelis, O Kolesnikova, H Kazakova, I Brandina, P Kamenski, R P Martin, I Tarassov","doi":"10.1007/978-1-4615-0721-5_9","DOIUrl":"https://doi.org/10.1007/978-1-4615-0721-5_9","url":null,"abstract":"<p><p>Mitochondria import from the cytoplasm the vast majority of proteins and some RNAs. Although there exists extended knowledge concerning the mechanisms of protein import, the import of RNA is poorly understood. It was almost exclusively studied on the model of tRNA import, in several protozoans, plants and yeast. Mammalian mitochondria, which do not import tRNAs naturally, are hypothesized to import other small RNA molecules from the cytoplasm. We studied tRNA import in the yeast system, both in vitro and in vivo, and applied similar approaches to study 5S rRNA import into human mitochondria. Despite the obvious divergence of RNA import systems suggested for different species, we find that in yeast and human cells this pathway involves similar mechanisms exploiting cytosolic proteins to target the RNA to the organelle and requiring the integrity of pre-protein import apparatus. The import pathway might be of interest from a biomedical point of view, to target into mitochondria RNAs that could suppress pathological mutations in mitochondrial DNA. Yeast represents a good model to elaborate such a gene therapy approach. We have described here the various approaches and protocols to study RNA import into mitochondria of yeast and human cells in vitro and in vivo.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"24 ","pages":"191-213"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0721-5_9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22096346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}