{"title":"Biosynthesis and metabolism of glutathione in plants.","authors":"Melinda Neal Martin","doi":"10.1007/978-1-4615-0073-5_8","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_8","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"163-88"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural analyses of living plant nuclei.","authors":"Naohiro Kato","doi":"10.1007/978-1-4615-0073-5_4","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_4","url":null,"abstract":"<p><p>The nucleus is the cellular organelle in which the bulk of the genomic information is stored. From studies using fluorescence microscopy with optical sections of fixed cells, a picture of an organized nuclear structure has emerged. Recently, the application of the green fluorescent protein (GFP) as a fluorescent dye allows the visualization of nuclear dynamics in live cells. Using four-dimensional fluorescence microscopy, the nuclear structures within an interphase nucleus are perceived to have dynamic domains. Structural analyses of a living plant nucleus contribute to our understanding of the genome information process in a particular cell in multicelluar systems.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"65-90"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Delitto perfetto targeted mutagenesis in yeast with oligonucleotides.","authors":"Francesca Storici, Michael A Resnick","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"189-207"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of targeted viral vectors for cardiovascular gene therapy.","authors":"Stuart A Nicklin, Andrew H Baker","doi":"10.1007/978-1-4615-0073-5_2","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_2","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"15-49"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Bacterial ion channels.","authors":"Ian R Booth","doi":"10.1007/978-1-4615-0073-5_5","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_5","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"91-111"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Functional analysis of promoter elements in plants.","authors":"Slavko Komarnytsky, Nikolai Borisjuk","doi":"10.1007/978-1-4615-0073-5_6","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_6","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"113-41"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shi-You Ding, Raphael Lamed, Edward A Bayer, Michael E Himmel
{"title":"The bacterial scaffoldin: structure, function and potential applications in the nanosciences.","authors":"Shi-You Ding, Raphael Lamed, Edward A Bayer, Michael E Himmel","doi":"10.1007/978-1-4615-0073-5_10","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_10","url":null,"abstract":"<p><p>Natural protein complexes may provide the best templates for nanometer-scale technology and new biomaterials. The bacterial cellulosome is becoming a well-understood multi-protein complex found in cellulolytic microorganisms. The scaffoldin subunits of the bacterial cellulosome function to organize and position other protein subunits into the complex. The scaffoldins can also serve as an attachment device for harnessing the cellulosome onto the cell surface and/or for its targeting to substrate. Biochemical and molecular biological evidence have identified a receptor/adaptor type of protein domain pair, called \"cohesin and dockerin,\" which is responsible for cellulosome self-assembly. The recognition between cohesin and dockerin is generally type and/or species specific. More than 80 cohesin and 100 dockerin sequences have been found, mostly from anaerobic bacteria. X-ray crystallography and NMR have been used to determine the three-dimensional structures of representative cohesin and dockerin domains, respectively. The cohesin peptide is about 140 amino acids in length and highly conserved in sequence and domain structure. The dockerin domain comprises about 70 amino acids and contains two 22 amino acid duplicated regions, each of which includes an \"F-hand\" modification of the EF-hand calcium-binding motif. Biochemical evidence and site-directed mutagenesis have confirmed that the two F-hand motifs are required for function and calcium dependence; at least two amino acids from each motif are critical for cohesin-dockerin recognition. In this report, we review the structure and function of the scaffoldin of the bacterial cellulosome and emphasize a detailed sequence analysis of the cohesin and dockerin domains. We also speculate about potential applications in nanoscience that may be based on cohesin-dockerin recognition.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"209-25"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dong Xu, Dongsup Kim, Phuongan Dam, Manesh Shah, Edward C Uberbacher, Ying Xu
{"title":"Characterization of protein structure and function at genome scale with a computational prediction pipeline.","authors":"Dong Xu, Dongsup Kim, Phuongan Dam, Manesh Shah, Edward C Uberbacher, Ying Xu","doi":"10.1007/978-1-4615-0073-5_12","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_12","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"269-93"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}