Genetic engineering最新文献

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Protein sequence database methods. 蛋白质序列数据库方法。
Genetic engineering Pub Date : 2004-01-01 DOI: 10.1007/978-0-306-48573-2_2
M. Martin, C. O’Donovan, R. Apweiler
{"title":"Protein sequence database methods.","authors":"M. Martin, C. O’Donovan, R. Apweiler","doi":"10.1007/978-0-306-48573-2_2","DOIUrl":"https://doi.org/10.1007/978-0-306-48573-2_2","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"42 1","pages":"13-7"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"51664591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biosynthesis and metabolism of glutathione in plants. 植物谷胱甘肽的生物合成与代谢。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_8
Melinda Neal Martin
{"title":"Biosynthesis and metabolism of glutathione in plants.","authors":"Melinda Neal Martin","doi":"10.1007/978-1-4615-0073-5_8","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_8","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"163-88"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Structural analyses of living plant nuclei. 活植物细胞核的结构分析。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_4
Naohiro Kato
{"title":"Structural analyses of living plant nuclei.","authors":"Naohiro Kato","doi":"10.1007/978-1-4615-0073-5_4","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_4","url":null,"abstract":"<p><p>The nucleus is the cellular organelle in which the bulk of the genomic information is stored. From studies using fluorescence microscopy with optical sections of fixed cells, a picture of an organized nuclear structure has emerged. Recently, the application of the green fluorescent protein (GFP) as a fluorescent dye allows the visualization of nuclear dynamics in live cells. Using four-dimensional fluorescence microscopy, the nuclear structures within an interphase nucleus are perceived to have dynamic domains. Structural analyses of a living plant nucleus contribute to our understanding of the genome information process in a particular cell in multicelluar systems.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"65-90"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Delitto perfetto targeted mutagenesis in yeast with oligonucleotides. 酵母寡核苷酸靶向诱变研究。
Genetic engineering Pub Date : 2003-01-01
Francesca Storici, Michael A Resnick
{"title":"Delitto perfetto targeted mutagenesis in yeast with oligonucleotides.","authors":"Francesca Storici,&nbsp;Michael A Resnick","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"189-207"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hybrid peptide-polyketide natural products: biosynthesis and prospects towards engineering novel molecules. 多肽-聚酮杂化天然产物:生物合成及工程新分子的前景。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_11
Liangcheng Du, Yi-Qiang Cheng, Gudrun Ingenhorst, Gong-Li Tang, Yong Huang, Ben Shen
{"title":"Hybrid peptide-polyketide natural products: biosynthesis and prospects towards engineering novel molecules.","authors":"Liangcheng Du,&nbsp;Yi-Qiang Cheng,&nbsp;Gudrun Ingenhorst,&nbsp;Gong-Li Tang,&nbsp;Yong Huang,&nbsp;Ben Shen","doi":"10.1007/978-1-4615-0073-5_11","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_11","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"227-67"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 17
Development of targeted viral vectors for cardiovascular gene therapy. 心血管基因治疗靶向病毒载体的研究进展。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_2
Stuart A Nicklin, Andrew H Baker
{"title":"Development of targeted viral vectors for cardiovascular gene therapy.","authors":"Stuart A Nicklin,&nbsp;Andrew H Baker","doi":"10.1007/978-1-4615-0073-5_2","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_2","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"15-49"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Bacterial ion channels. 细菌离子通道。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_5
Ian R Booth
{"title":"Bacterial ion channels.","authors":"Ian R Booth","doi":"10.1007/978-1-4615-0073-5_5","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_5","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"91-111"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Functional analysis of promoter elements in plants. 植物启动子元件的功能分析。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_6
Slavko Komarnytsky, Nikolai Borisjuk
{"title":"Functional analysis of promoter elements in plants.","authors":"Slavko Komarnytsky,&nbsp;Nikolai Borisjuk","doi":"10.1007/978-1-4615-0073-5_6","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_6","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"113-41"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-4615-0073-5_6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 25
The bacterial scaffoldin: structure, function and potential applications in the nanosciences. 细菌支架蛋白:结构、功能及其在纳米科学中的潜在应用。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_10
Shi-You Ding, Raphael Lamed, Edward A Bayer, Michael E Himmel
{"title":"The bacterial scaffoldin: structure, function and potential applications in the nanosciences.","authors":"Shi-You Ding,&nbsp;Raphael Lamed,&nbsp;Edward A Bayer,&nbsp;Michael E Himmel","doi":"10.1007/978-1-4615-0073-5_10","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_10","url":null,"abstract":"<p><p>Natural protein complexes may provide the best templates for nanometer-scale technology and new biomaterials. The bacterial cellulosome is becoming a well-understood multi-protein complex found in cellulolytic microorganisms. The scaffoldin subunits of the bacterial cellulosome function to organize and position other protein subunits into the complex. The scaffoldins can also serve as an attachment device for harnessing the cellulosome onto the cell surface and/or for its targeting to substrate. Biochemical and molecular biological evidence have identified a receptor/adaptor type of protein domain pair, called \"cohesin and dockerin,\" which is responsible for cellulosome self-assembly. The recognition between cohesin and dockerin is generally type and/or species specific. More than 80 cohesin and 100 dockerin sequences have been found, mostly from anaerobic bacteria. X-ray crystallography and NMR have been used to determine the three-dimensional structures of representative cohesin and dockerin domains, respectively. The cohesin peptide is about 140 amino acids in length and highly conserved in sequence and domain structure. The dockerin domain comprises about 70 amino acids and contains two 22 amino acid duplicated regions, each of which includes an \"F-hand\" modification of the EF-hand calcium-binding motif. Biochemical evidence and site-directed mutagenesis have confirmed that the two F-hand motifs are required for function and calcium dependence; at least two amino acids from each motif are critical for cohesin-dockerin recognition. In this report, we review the structure and function of the scaffoldin of the bacterial cellulosome and emphasize a detailed sequence analysis of the cohesin and dockerin domains. We also speculate about potential applications in nanoscience that may be based on cohesin-dockerin recognition.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"209-25"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Characterization of protein structure and function at genome scale with a computational prediction pipeline. 用计算预测管道在基因组尺度上表征蛋白质结构和功能。
Genetic engineering Pub Date : 2003-01-01 DOI: 10.1007/978-1-4615-0073-5_12
Dong Xu, Dongsup Kim, Phuongan Dam, Manesh Shah, Edward C Uberbacher, Ying Xu
{"title":"Characterization of protein structure and function at genome scale with a computational prediction pipeline.","authors":"Dong Xu,&nbsp;Dongsup Kim,&nbsp;Phuongan Dam,&nbsp;Manesh Shah,&nbsp;Edward C Uberbacher,&nbsp;Ying Xu","doi":"10.1007/978-1-4615-0073-5_12","DOIUrl":"https://doi.org/10.1007/978-1-4615-0073-5_12","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"25 ","pages":"269-93"},"PeriodicalIF":0.0,"publicationDate":"2003-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24620135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
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