Genetic engineering最新文献

{"title":"Nucleic Acids","authors":"W. Hill","doi":"10.1201/9780429102233-3","DOIUrl":"https://doi.org/10.1201/9780429102233-3","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46110343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Other Applications For Gene Therapy","authors":"W. Hill","doi":"10.1201/9780429102233-12","DOIUrl":"https://doi.org/10.1201/9780429102233-12","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49212233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gene Therapy and Disease","authors":"W. Hill","doi":"10.1201/9780429102233-11","DOIUrl":"https://doi.org/10.1201/9780429102233-11","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43044621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Placing New Genes in Mammalian Cells","authors":"W. Hill","doi":"10.1201/9780429102233-8","DOIUrl":"https://doi.org/10.1201/9780429102233-8","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65944412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Presentation of Dolly the Sheep and Human Cloning in the Mass Media","authors":"Miguel Alcíbar","doi":"10.5772/56161","DOIUrl":"https://doi.org/10.5772/56161","url":null,"abstract":"He watched her drift away, drift with her pink face warm, smoothas an apple, unwrinkled and colorful. She chimed her laugh at everyjoke, she tossed salads neatly, never once pausing for breath. And thebony son and curved daughters were brilliantly witty, like theirfather, telling of the long years and their secret life, while theirfather nodded proudly to each.(“The Long Years”, Martian Chronicles, Ray Brad‐ bury)On the next day, John saw Jesus coming toward him,and so he said: “Behold, the Lamb of God. Behold, he whotakes away the sin of the world”.(John 1, 29)","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"20 1","pages":"103-128"},"PeriodicalIF":0.0,"publicationDate":"2013-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/56161","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70951407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Engineering and Cloning: Focus on Animal Biotechnology","authors":"M. I. Giassetti, F. S. Maria, M. E. O. A. Assumpção, J. A. Visintin","doi":"10.5772/56071","DOIUrl":"https://doi.org/10.5772/56071","url":null,"abstract":"Over the last 35 years the term genetic engineering has been commonly used not only in science but also in others parts of society. Nowadays this name is often associated by the media forensic techniques to solve crimes, paternity, medical diagnosis and, gene mapping and sequencing. The popularization of genetic engineering is consequence of its wide use in laboratories around the world and, developing of modern and efficient techniques. The genetic engineering, often used with trivia, involves sophisticated techniques of gene manipulation, cloning and modification. Many authors consider this term as synonymous as genetic modification, where a synthetic gene or foreign DNA is inserted into an organism of interest. Organism that receives this recombinant DNA is considered as genetically modified (GMO). Its production are summarized in simplified form in five steps: 1) Isolation of interested gene, 2) Construction, gene of interested is joined with promoters (location and control the level of expression), terminator (indicates end of the gene) and expression marker (identify the gene expression), 3) transformation (when the recombinant DNA is inserted into the host organism), 4) Selection (selection of those organisms that express the markers), 5) Insertion verification of recombinant DNA and its expression [1].","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2013-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5772/56071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70950813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A high-throughput approach to protein structure analysis.","authors":"Babu A Manjasetty, Wuxian Shi, Chenyang Zhan, András Fiser, Mark R Chance","doi":"10.1007/978-0-387-34504-8_7","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_7","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"105-28"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New mass-spectrometry-based strategies for lipids.","authors":"Giorgis Isaac,&nbsp;Richard Jeannotte,&nbsp;Steven Wynn Esch,&nbsp;Ruth Welti","doi":"10.1007/978-0-387-34504-8_8","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_8","url":null,"abstract":"<p><p>In the past dozen years, many new strategies for mass-spectrometry-based analyses of lipids have been developed. Lipidomics has emerged as a comprehensive approach to analysis of lipids from biological systems, and the most-utilized lipidomics methodologies involve electrospray ionization (ESI) sources and triple quadrupole analyzers. While mass spectral techniques for lipid profiling have advanced, challenges in developing uniform data acquisition methods and in handling, storing, and analyzing mass spectral data remain. Investigation of other ionization methods, including matrix-assisted laser desorption/ionization (MALDI) and atmospheric pressure chemical ionization (APCI), has demonstrated that these are useful in specific applications. APCI is particularly amenable to analysis of less polar lipids, and MALDI provides a rapid technology with application for tissue imaging. Time-of-flight secondary ion mass spectrometry (TOF-SIMS) is particularly suited for imaging of tissues and cells.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"129-57"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paired-end genomic signature tags: a method for the functional analysis of genomes and epigenomes.","authors":"John J Dunn,&nbsp;Sean R McCorkle,&nbsp;Logan Everett,&nbsp;Carl W Anderson","doi":"10.1007/978-0-387-34504-8_9","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_9","url":null,"abstract":"<p><p>Because paired-end genomic signature tags are sequenced-based, they have the potential to become an alternate tool to tiled microarray hybridization as a method for genome-wide localization of transcription factors and other sequence-specific DNA binding proteins. As outlined here the method also can be used for global analysis of DNA methylation. One advantage of this approach is the ability to easily switch between different genome types without having to fabricate a new microarray for each and every DNA type. However, the method does have some disadvantages. Among the most rate-limiting steps of our PE-GST protocol are the need to concatemerize the diTAGs, size fractionate them and then clone them prior to sequencing. This is usually followed by additional steps to amplify and size select for long (> or = 500) concatemer inserts prior to sequencing. These time-consuming steps are important for standard DNA sequencing as they increase efficiency approximately 20-30-fold since each amplified concatemer can now provide information on multiple tags; the limitation on data acqui- sition is read length during sequencing. However, the development of new sequencing methods such as Life Sciences' 454 new nanotechnology-based sequencing instrument (41) could increase tag sequencing efficiency by several orders of magnitude (> or = 100,000 diTAG reads/run), which is sufficient to provide in-depth global analysis of all ChIP PE-GSTs in a single run. This is because the lengths of our paired-end diTAGs (approximately 60 bp) fall well within the region of high accuracy for read lengths on this instrument. In principle, sequence analysis of diTAGs could begin as soon as they are generated, thereby completely bypassing the need for the concatemerization, sizing, downstream cloning steps and sequencing template purification. In addition, our protocol places any one of several unique four-base long nucleotide sequences, such as GATC, between each and every diTAG pair, which could be used to help the instrument's software keep base register and also provide a well-located peak height indicator in the middle of every sequence run. This additional feature could permit multiplexing of the data by simultaneous sequencing of several pooled libraries if each used a different linker sequence during diTAG formation (Figure 4).</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"159-73"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Root-knot and cyst nematode parasitism genes: the molecular basis of plant parasitism.","authors":"Thomas J Baum,&nbsp;Richard S Hussey,&nbsp;Eric L Davis","doi":"10.1007/978-0-387-34504-8_2","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_2","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"17-43"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}