Genetic engineering最新文献

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Regulation of plant intercellular communication via plasmodesmata. 植物胞间连丝通讯的调控。
Genetic engineering Pub Date : 2007-01-01 DOI: 10.1007/978-0-387-34504-8_1
Insoon Kim, Ken Kobayashi, Euna Cho, Patricia C Zambryski
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引用次数: 7
Proximity ligation: a specific and versatile tool for the proteomic era. 近距离结扎:蛋白质组学时代的一种特殊而通用的工具。
Genetic engineering Pub Date : 2007-01-01 DOI: 10.1007/978-0-387-34504-8_5
Ola Söderberg, Karl-Johan Leuchowius, Masood Kamali-Moghaddam, Malin Jarvius, Sigrun Gustafsdottir, Edith Schallmeiner, Mats Gullberg, Jonas Jarvius, Ulf Landegren
{"title":"Proximity ligation: a specific and versatile tool for the proteomic era.","authors":"Ola Söderberg,&nbsp;Karl-Johan Leuchowius,&nbsp;Masood Kamali-Moghaddam,&nbsp;Malin Jarvius,&nbsp;Sigrun Gustafsdottir,&nbsp;Edith Schallmeiner,&nbsp;Mats Gullberg,&nbsp;Jonas Jarvius,&nbsp;Ulf Landegren","doi":"10.1007/978-0-387-34504-8_5","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_5","url":null,"abstract":"<p><p>Knowledge about the total human genome sequence now provides opportunities to study its myriad gene products. However, the presence of alternative splicing, post-translational modifications, and innumerable protein-protein interactions among proteins occurring at widely different concentrations, all combine to place extreme demands on the specificity and sensitivity of assays. The choice of method also depends on matters such as whether proteins will be analyzed in body fluids and lysates, or localized inside single cells. In this review we discuss commonly used detection methods and compare these to the recently-developed proximity ligation technique.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"85-93"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 55
Mutagenesis of human p53 tumor suppressor gene sequences in embryonic fibroblasts of genetically-engineered mice. 人p53肿瘤抑制基因序列在基因工程小鼠胚胎成纤维细胞中的突变。
Genetic engineering Pub Date : 2007-01-01 DOI: 10.1007/978-0-387-34504-8_3
Zhipei Liu, Djeda Belharazem, Karl Rudolf Muehlbauer, Tatiana Nedelko, Yuri Knyazev, Monica Hollstein
{"title":"Mutagenesis of human p53 tumor suppressor gene sequences in embryonic fibroblasts of genetically-engineered mice.","authors":"Zhipei Liu,&nbsp;Djeda Belharazem,&nbsp;Karl Rudolf Muehlbauer,&nbsp;Tatiana Nedelko,&nbsp;Yuri Knyazev,&nbsp;Monica Hollstein","doi":"10.1007/978-0-387-34504-8_3","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_3","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"45-54"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 15
Salicylic acid-, jasmonic acid- and ethylene-mediated regulation of plant defense signaling. 水杨酸、茉莉酸和乙烯介导的植物防御信号调控。
Genetic engineering Pub Date : 2007-01-01 DOI: 10.1007/978-0-387-34504-8_4
Aardra Kachroo, Pradeep Kachroo
{"title":"Salicylic acid-, jasmonic acid- and ethylene-mediated regulation of plant defense signaling.","authors":"Aardra Kachroo,&nbsp;Pradeep Kachroo","doi":"10.1007/978-0-387-34504-8_4","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_4","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"55-83"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 73
Protein overexpression in mammalian cell lines. 哺乳动物细胞系中蛋白质的过度表达。
Genetic engineering Pub Date : 2007-01-01 DOI: 10.1007/978-0-387-34504-8_6
Paul Freimuth
{"title":"Protein overexpression in mammalian cell lines.","authors":"Paul Freimuth","doi":"10.1007/978-0-387-34504-8_6","DOIUrl":"https://doi.org/10.1007/978-0-387-34504-8_6","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"28 ","pages":"95-104"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-0-387-34504-8_6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26429933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Anionic nutrient transport in plants: the molecular basis of the sulfate transporter gene family. 植物阴离子营养转运:硫酸盐转运基因家族的分子基础。
Genetic engineering Pub Date : 2006-01-01 DOI: 10.1007/0-387-25856-6_5
Hideki Takahashi, Naoko Yoshimoto, Kazuki Saito
{"title":"Anionic nutrient transport in plants: the molecular basis of the sulfate transporter gene family.","authors":"Hideki Takahashi,&nbsp;Naoko Yoshimoto,&nbsp;Kazuki Saito","doi":"10.1007/0-387-25856-6_5","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_5","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"67-80"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Biogenesis of iron-sulfur cluster proteins in plastids. 铁硫簇蛋白在质体中的生物发生。
Genetic engineering Pub Date : 2006-01-01 DOI: 10.1007/0-387-25856-6_7
Marinus Pilon, Salah E Abdel-Ghany, Douglas Van Hoewyk, Hong Ye, Elizabeth A H Pilon-Smits
{"title":"Biogenesis of iron-sulfur cluster proteins in plastids.","authors":"Marinus Pilon,&nbsp;Salah E Abdel-Ghany,&nbsp;Douglas Van Hoewyk,&nbsp;Hong Ye,&nbsp;Elizabeth A H Pilon-Smits","doi":"10.1007/0-387-25856-6_7","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_7","url":null,"abstract":"<p><p>Iron-sulfur (Fe-S) clusters are co-factors of proteins that perform a number of biological roles, including electron transfer, redox and non-redox catalysis, regulation of gene expression, and as sensors within all living organisms, prokaryotes and eukaryotes. These clusters are thought to be among the oldest structures found in biological cells. In chloroplasts, Fe-S clusters play a key role in photosynthetic electron transport as well as nitrogen and sulfur assimilation. The capacity of the Fe atom in Fe-S clusters to take up an electron reversibly provides the required electron carrier capacity in these pathways. Iron and sulfur limitation both affect plant primary production and growth. It has long been known that iron deficiency leads to defects in photosynthesis and bleaching in young leaves, phenomena that are closely linked to a defect in chloroplastic photosystem-I (PSI) accumulation, a major Fe-S containing protein complex in plants. Although the functional importance of Fe-S cluster proteins is evident and isolated chloroplasts have been shown to be able to synthesize their own Fe-S clusters, much is yet to be learned about the biosynthesis of Fe-S proteins in plastids. The recent discovery of a NifS-like protein in plastids has hinted to the existence of an assembly machinery related to bacterial Fe-S assembly systems. This chapter aims to summarize what we presently know about the assembly of Fe-S clusters in plants with an emphasis on green plastids.</p>","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"101-17"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 35
Phospholipid-derived signaling in plant response to temperature and water stresses. 植物对温度和水分胁迫反应中的磷脂来源信号。
Genetic engineering Pub Date : 2006-01-01 DOI: 10.1007/0-387-25856-6_4
Xuemin Wang
{"title":"Phospholipid-derived signaling in plant response to temperature and water stresses.","authors":"Xuemin Wang","doi":"10.1007/0-387-25856-6_4","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_4","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"57-66"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Molecular roles of chaperones in assisted folding and assembly of proteins. 伴侣蛋白在蛋白质折叠和组装中的分子作用。
Genetic engineering Pub Date : 2006-01-01 DOI: 10.1007/0-387-25856-6_11
Mark T Fisher
{"title":"Molecular roles of chaperones in assisted folding and assembly of proteins.","authors":"Mark T Fisher","doi":"10.1007/0-387-25856-6_11","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_11","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"191-229"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25775470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Identification and analysis of microRNAs. microrna的鉴定和分析。
Genetic engineering Pub Date : 2006-01-01 DOI: 10.1007/0-387-25856-6_1
Shveta Bagga, Amy E Pasquinelli
{"title":"Identification and analysis of microRNAs.","authors":"Shveta Bagga,&nbsp;Amy E Pasquinelli","doi":"10.1007/0-387-25856-6_1","DOIUrl":"https://doi.org/10.1007/0-387-25856-6_1","url":null,"abstract":"","PeriodicalId":77144,"journal":{"name":"Genetic engineering","volume":"27 ","pages":"1-20"},"PeriodicalIF":0.0,"publicationDate":"2006-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/0-387-25856-6_1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25774366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 34
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