Biotherapy (Dordrecht, Netherlands)最新文献

Regulatory T Cells 调节性T细胞

Biotherapy (Dordrecht, Netherlands) Pub Date : 2016-02-22 DOI: 10.1007/978-4-431-55031-0_21

H. Onishi, T. Morisaki, M. Katano

引用次数: 0

Mammalian target of rapamycin（mTOR）阻害薬 rapamycin (mTOR)抑制剂

Biotherapy (Dordrecht, Netherlands) Pub Date : 2011-01-01 DOI: 10.1007/978-3-319-67199-4_102143

辛島尚

引用次数: 19

Natural and therapeutically-induced antibodies to cytokines. 天然和治疗诱导的细胞因子抗体。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1007/BF02678552

R P Revoltella

引用次数: 24

Immune responses to stress proteins: applications to infectious disease and cancer. 对应激蛋白的免疫反应:在传染病和癌症中的应用。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1007/BF02678295

L Mizzen

{"title":"Immune responses to stress proteins: applications to infectious disease and cancer.","authors":"L Mizzen","doi":"10.1007/BF02678295","DOIUrl":"https://doi.org/10.1007/BF02678295","url":null,"abstract":"Heat shock proteins, or stress proteins have been identified as part of a highly conserved cellular defence mechanism mediated by multiple, distinct gene families and corresponding gene products. As intracellular chaperones, stress proteins participate in many essential biochemical pathways of protein maturation and function active during times of stress and during normal cellular homeostasis. In addition to their well-characterized role as protein chaperones, stress proteins are now realized to possess another important biological property: immunogenicity. Stress proteins are now understood to play a fundamental role in immune surveillance of infection and malignancy and this body of basic research has provided a framework for their clinical application. As key targets of both humoral and cellular immunity during infection, stress proteins have accordingly received considerable research interest as prophylactic vaccines for infectious disease applications. The unique and potent immunostimulatory properties of stress proteins have similarly been applied to the development of new approaches to cancer therapy, including both protein and gene-based modalities.","PeriodicalId":77043,"journal":{"name":"Biotherapy (Dordrecht, Netherlands)","volume":"10 3","pages":"173-89"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02678295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20481103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Comparison of conventional immunoassays (RIA, ELISA) with surface plasmon resonance for pesticide detection and monitoring. 传统免疫分析法(RIA、ELISA)与表面等离子体共振法在农药检测和监测中的比较。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1023/a:1007930112221

R P Revoltella, L Laricchia Robbio, B Liedberg

引用次数: 17

Cytokine effect on ex vivo expansion of haemopoietic stem cells from different human sources. 细胞因子对不同人造血干细胞体外扩增的影响。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1023/a:1008081708054

S Eridani, U Mazza, P Massaro, M L La Targia, A T Maiolo, A Mosca

{"title":"Cytokine effect on ex vivo expansion of haemopoietic stem cells from different human sources.","authors":"S Eridani, U Mazza, P Massaro, M L La Targia, A T Maiolo, A Mosca","doi":"10.1023/a:1008081708054","DOIUrl":"https://doi.org/10.1023/a:1008081708054","url":null,"abstract":"Human pluripotential stem cells (PSC) are currently the target for transplantation attempts and genetic manipulation. We have therefore investigated the frequency and the expansion potential of PSC's in different types of blood samples. CD 34+ cells were thus obtained from human bone marrow (BM), as well as from peripheral blood (PB) and cord blood (CB) samples. After immuno-magnetic separation the highest yields of CD 34+ cells were from BM (1.08-2.25%) and CB (0.42-1.32%) while PB samples gave much lower values. Suspension cultures of PSC's from the three sources were then set up, in the presence of combinations of haemopoietic growth factors. A remarkable amplification of the nucleated cell pool was observed reaching a maximum between 10 and 15 days of culture; earliest and maximum expansion (up to 220-fold) was achieved when Erythropoietin (Epo) was added to the culture medium, but this resulted in reduction of colony-forming cells and differentiation into erythroid progenitors. Clonogenic tests for BFU-E's derived colonies showed a peak value at 5 days of liquid culture. Further studies are advisable to establish the best cytokine combination for a valuable ex vivo expansion, coupled with preservation of stem cell properties.","PeriodicalId":77043,"journal":{"name":"Biotherapy (Dordrecht, Netherlands)","volume":"11 4","pages":"291-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1008081708054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20855797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Heat shock proteins and the antitumor T cell response. 热休克蛋白与抗肿瘤T细胞反应。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1007/BF02678301

M Harada, G Kimura, K Nomoto

{"title":"Heat shock proteins and the antitumor T cell response.","authors":"M Harada, G Kimura, K Nomoto","doi":"10.1007/BF02678301","DOIUrl":"https://doi.org/10.1007/BF02678301","url":null,"abstract":"Heat shock proteins (HSP) have been shown to participate in the antitumor T cell response. First, HSP play a crucial role in the intracellular pathway for antigen processing where HSP can make complexes with a broad spectrum of cellular proteins and peptides through their chaperone functions. In this pathway, macrophages are required for processing the chaperoned peptides to make stable molecules with the major histocompatibility complex (MHC) class I molecules, even when HSP-peptide complexes are exogenously administered. Through this pathway, vaccination with HSP-peptide complexes is thus able to elicit the response of CD8+ T cells specific for the chaperoned peptides. These findings suggest an essential role of HSP in 'cross-priming' and their usefulness for antitumor vaccination with tumor peptides. Second, HSP have been suggested to be expressed on the cell surface by transformation and, in addition, to function as antigen-presenting molecules for double negative T cells. Third, HSP derived from tumor cells have reportedly been recognized by T cells with either T cell receptor (TCR)-alphabeta or TCR-gammadelta. These lines of evidence therefore indicate that HSP may be potentially promising target molecules for antitumor T cell immunotherapy.","PeriodicalId":77043,"journal":{"name":"Biotherapy (Dordrecht, Netherlands)","volume":"10 3","pages":"229-35"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02678301","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20481084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

HLA-DR/DQ transgenic, class II deficient mice as a novel model to select for HSP T cell epitopes with immunotherapeutic or preventative vaccine potential. HLA-DR/DQ转基因II类缺陷小鼠作为一种新的模型来选择具有免疫治疗或预防疫苗潜力的热休克T细胞表位。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1007/BF02678296

A Geluk, V Taneja, K E van Meijgaarden, R R de Vries, C S David, T H Ottenhoff

{"title":"HLA-DR/DQ transgenic, class II deficient mice as a novel model to select for HSP T cell epitopes with immunotherapeutic or preventative vaccine potential.","authors":"A Geluk, V Taneja, K E van Meijgaarden, R R de Vries, C S David, T H Ottenhoff","doi":"10.1007/BF02678296","DOIUrl":"https://doi.org/10.1007/BF02678296","url":null,"abstract":"Protective immunity against mycobacteria is dependent on antigen/MHC class II specific, CD4+ Th1 cells. HLA-DR3-restricted Th1 cells respond to a subset of mycobacterial antigens, including the immunodominant hsp65, and recognize a single epitope in hsp65, notably p1-20. Altered peptide ligands (APL) of p1-20 can inhibit p1-20/hsp65-induced proliferation of DR3-restricted T cells in an allele specific manner in vitro. In order to develop a preclinical model in which p1-20 APL can be tested in vivo in the context of HLA, we have used murine class II deficient, HLA transgenic (Ab0) mice, in which all CD4+ T cells are restricted by the tg HLA molecule. BCG-immunized DR3.Ab0 and DQ8.Ab0 mice both responded well to hsp65. Furthermore, DR3.Ab0 mice recognized precisely the same p1-20 epitope as DR3-restricted human T cells, whereas DQ8.Ab0 mice responded to a different set of hsp65 peptides. This shows that (i) the same immunodominant protein and peptide epitope are recognized by T cells from DR3.Ab0 mice and DR3+ humans and (ii) indicates the major role of HLA-polymorphism in controlling the human T cell response to mycobacterial antigens. Thus, HLA-transgenic, Ab0 mice provide a novel, preclinical model system to analyze APL and vaccines in the context of HLA polymorphism.","PeriodicalId":77043,"journal":{"name":"Biotherapy (Dordrecht, Netherlands)","volume":"10 3","pages":"191-6"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02678296","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20481104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

The role of homeobox genes in hematopoiesis. 同源盒基因在造血中的作用。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1007/BF02678548

M C Magli

引用次数: 12

Multi-compartmental models of contaminant fate in the environment. 环境中污染物命运的多区隔模型。

Biotherapy (Dordrecht, Netherlands) Pub Date : 1998-01-01 DOI: 10.1023/a:1007936823608

F Wania

引用次数: 9