Biological structures and morphogenesis最新文献_第5页

In vitro proliferation and differentiation of myogenic cells from adult Xenopus. 成年非洲爪蟾成肌细胞的体外增殖与分化。

Biological structures and morphogenesis Pub Date : 1988-01-01

R Franquinet, A Aamiri, J Gautron, A Le Moigne

{"title":"In vitro proliferation and differentiation of myogenic cells from adult Xenopus.","authors":"R Franquinet, A Aamiri, J Gautron, A Le Moigne","doi":"","DOIUrl":"","url":null,"abstract":"A technique is described for isolating amphibian myogenic cells from the muscle of adult Xenopus laevis (Dauchin). Muscles were dissociated with 0.2% collagenase and 0.1% trypsin. The resulting cell suspensions were separated from the remaining myofibres by filtration through nylon grids. Most of the cells remaining in the filtrate suspension were satellite cells or fibroblasts. When plated in Petri dishes, satellite cells adhered to the substrate, became spindle-shaped and proliferated activity in a culture medium supplemented with fetal calf serum. Mitotic waves lasted 4 days and consequently cell density markedly increased. Satellite cells came into contact and began to fuse into myotubes on day 8 of culture. Horse serum, which replaced fetal calf serum in the medium on day 12, accelerated cell fusions which were almost complete on day 18. However, under these conditions, some mononucleated cells continued to undergo mitosis. Cell proliferation with a high rate of mitosis was prolonged by repeated trypsinization and replating in medium supplemented with fetal calf serum. When myofibres from dissociated muscles were cultured under the same conditions, they never fragmented or divided.","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"1 2","pages":"84-8"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14534004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative histochemistry of the mucoproteic cells of the hypophysis from Rhamdia hilarii, Hypostomus punctatus, Prochilodus scrofa and Cyprinus carpio (Teleostei). Immunohistochemical identification of the gonadotropic cells. 鼠脚草、马尾蛇、山茱萸和鲤鱼垂体黏液蛋白细胞的组织化学比较。促性腺细胞的免疫组化鉴定。

Biological structures and morphogenesis Pub Date : 1988-01-01

M V Val-Sella, F Fava-De-Moraes, K Wakabayashi

{"title":"Comparative histochemistry of the mucoproteic cells of the hypophysis from Rhamdia hilarii, Hypostomus punctatus, Prochilodus scrofa and Cyprinus carpio (Teleostei). Immunohistochemical identification of the gonadotropic cells.","authors":"M V Val-Sella, F Fava-De-Moraes, K Wakabayashi","doi":"","DOIUrl":"","url":null,"abstract":"Adenohypophyseal cells showing positive histochemical reactions for mucosubstances were classified as type I-IV in Hypostomus (Plecostomus) punctatus (Loricariidae), Rhamdia hilarii (Pimelolidae), Prochilodus scrofa (Prochilodontidae) and Cyprinus carpio (Cyprinidae) according to cell shape, size, cytological characteristics and adenohypophyseal distribution. Cell types I and II are common to the four species, with each cell type showing very similar cytological and histochemical characteristics, in spite of different adenohypophyseal distribution of cell type II, according to the teleost species. Type I cells are globular basophils located in the proximal pars ditalis and are positive to PAS and Alcian blue pH 2.5 (AB) reactions, showing cytoplasmic vacuoles and changes in granule concentration in the mature phase of the gonadal cycle. The smaller type II cells are fusiform or oval basophils exhibiting a strong AB reaction but also reacting to PAS. Type III cells are located in the pars intermedia showing PAS-positive reaction. Considering different teleost species, these cells exhibit some variations specially in relation to cell size and shape which are not detected in mature male C. carpio. Otherwise cell type IV is only present in the rostral pars distalis of P. scrofa. They are weakly basophilic and negative to PAS, reacting strongly to AB. Only cell type I showed unequivocally positive immunohistochemical results with anti-salmon gonadotropin.","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"1 4","pages":"137-41"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14370184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The ultrastructure of A, B and D pancreatic cells in normal and in diabetic ducks. 正常和糖尿病鸭胰腺A、B、D细胞的超微结构。

Biological structures and morphogenesis Pub Date : 1988-01-01

F Laurent, C Hindelang, M T Strosser, P Mialhe

{"title":"The ultrastructure of A, B and D pancreatic cells in normal and in diabetic ducks.","authors":"F Laurent, C Hindelang, M T Strosser, P Mialhe","doi":"","DOIUrl":"","url":null,"abstract":"Ultrastructurally and immunocytochemically identified A, B and D cells are highly concentrated in the splenic bulb of the duck pancreas. Ultrastructural features of normal A, B and D cells are similar in the duck and in other species so far studied. However, normal D cells present a striking characteristic, i.e. apical accumulation of dense bodies, which seem to derive from multivesicular bodies and are probably involved in a catabolic regulatory process. Subtotal pancreatectomy in the duck, leaving the splenic bulb and inducing transient diabetes, produces strong secretory stimulation of A and B cells, as indicated by the development of the rough endoplasmic reticulum and the Golgi apparatus and transient degranulation, more marked in B cells. Numerous B cells with degenerative aspects, observed after 12 days, seem to be exhausted following prolonged hyperstimulation: this could explain why diabetes reappears in some cases. In contrast, in D cells, functional inhibition after surgery is suggested by a dramatic increase in the number and size of the dense bodies, associated with a marked decrease in secretory vesicle storage. The morphological data correlate well with the previously reported evolution of plasma and pancreatic hormone concentration after surgery, and suggest that the normal inhibitory control of glucagon and insulin secretion by the local release of somatostatin might be reduced or suppressed during transient diabetes in subtotally depancreatized ducks.","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"1 1","pages":"34-42"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14174955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual phenotype of avian chimeric gonads with germinal and stromal cells of opposite genetic sexes. 具有相反遗传性别的生发细胞和基质细胞的禽类嵌合性腺的性表型。

Biological structures and morphogenesis Pub Date : 1988-01-01

K Hajji, C Martin, A Perramon, F Dieterlen-Lièvre

{"title":"Sexual phenotype of avian chimeric gonads with germinal and stromal cells of opposite genetic sexes.","authors":"K Hajji, C Martin, A Perramon, F Dieterlen-Lièvre","doi":"","DOIUrl":"","url":null,"abstract":"The respective roles of germinal and stromal cells in determining the sexual phenotype of the gonad were analyzed in chimeric gonads obtained by surgical recombination between young avian blastodiscs in ovo. Equivalent territories were exchanged between two blastodisc, in order that the germinal crescent and the gonad territory had a different origin (fig. 3). Embryos used for these experiments carried a sex linked pigment mutation, that made it possible to diagnose the genetic sexes of germ cells and stroma at the time when the gonad was retrieved for examination. On the basis of species, three types of combination were performed: chick germ cells in chick or quail stroma, quail germ cells in chick stroma. In each chimera, the genetic sexes of the two gonadal cell populations could be identical or opposite. However it appeared that the germ cell population was not always homogeneous. In some grafting schemes, ectopic germ cells, located outside the germinal crescent, contributed to the colonization of the experimental gonad. These germ cells were from the same territory as the stroma element of the gonad, i.e., they were of the same species and the same genetic sex. Whatever the case, in 87 chimeras that were studied, the sex phenotype of the gonads always corresponded to the genetic sex of the stroma. Thus the genetic sex of germ cells has no role in the sexual differentiation of the gonadal rudiments.","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"1 3","pages":"107-16"},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14325524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in location of type I collagen synthesis in two stages of fetal calf skin as revealed by in situ hybridization. 原位杂交显示胎牛皮肤两个阶段I型胶原合成位置的变化。

Biological structures and morphogenesis Pub Date : 1988-01-01

D Le Guellec, R Ouazana, R Garrone

引用次数: 0

The levator ani of the female rat: a suitable model for studying the effects of testosterone on the development of mammalian muscles. 雌性大鼠的肛提肌:研究睾酮对哺乳动物肌肉发育影响的合适模型。

Biological structures and morphogenesis Pub Date : 1988-01-01

C Tobin, Y Joubert

引用次数: 0

Lectins in histochemistry. A survey. 组织化学中的凝集素。一项调查。

Biological structures and morphogenesis Pub Date : 1988-01-01

A Danguy, R Kiss, J L Pasteels