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Biological structures and morphogenesis最新文献

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Fine structure of cells in the young regenerating spinal cord of the lizard Anolis carolinensis after H3-thymidine administration. h3 -胸苷嘧啶对卡罗莱纳蜥蜴幼龄再生脊髓细胞精细结构的影响。
Biological structures and morphogenesis Pub Date : 1992-01-01
L Alibardi, J Gibbons, S Simpson
{"title":"Fine structure of cells in the young regenerating spinal cord of the lizard Anolis carolinensis after H3-thymidine administration.","authors":"L Alibardi,&nbsp;J Gibbons,&nbsp;S Simpson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>H3-thymidine was injected in a single dose into adult lizards Anolis carolinensis during early stages of tail regeneration when the new tail measured in length 2-5 mm (about 3 weeks). Electron microscopic autoradiography revealed that in the regenerating spinal cord three different cell types were recognizable four hours post-injection. By far the most common type was the ependymal cell. Ependymal or roundish cells with clear electron-density or storing bundles of intermediate filaments were occasionally found. A second rarer, cell type devoid of intermediate filaments was a pale round cell, often highly labelled. This cell showed a very poor ultrastructural differentiation and probably represents the precursor of the few neurons and glial cells observed in older stages of regeneration. The third type was a neuroblast at different degrees of differentiation and did not uptake H3-thymidine revealing it was a post-mitotic cell. Despite the relatively advanced ultrastructural differentiation of these neurons, synapses were rarely seen after three weeks of SC regeneration in Anolis.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 2","pages":"45-52"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12659821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endocrinology of calcium metabolism in amphibians, with emphasis on the evolution of hypercalcemic regulation in tetrapods. 两栖动物钙代谢的内分泌学，重点是四足动物高钙调节的进化。
Biological structures and morphogenesis Pub Date : 1992-01-01
A K Pandey
{"title":"Endocrinology of calcium metabolism in amphibians, with emphasis on the evolution of hypercalcemic regulation in tetrapods.","authors":"A K Pandey","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Amphibians are the most diverse group as regards the endocrinology of Ca homeostasis because in them only \"true\" parathyroid glands made first appearance but its involvement varies greatly according to their phyletic affinities and habitats. Presence of ultimobranchial gland, parathyroid and renal 25-hydroxyvitamin-D-1-hydroxylase enzyme in anurans represents their tetrapod pattern of Ca regulation. Among \"higher\" urodeles, both parathyroid and pituitary are involved in hypercalcemic regulation whereas in \"lower\" urodeles (some of them are \"aparathyroid\" too), pituitary alone appears to discharge this function. The latter situation is analogous to the fish. Apodans do possess ultimobranchial and parathyroid glands but the problem of their Ca metabolism has not yet been explored. This review deals exhaustively with the endocrinology of Ca metabolism in amphibians. Also, the evolution of hypercalcemic regulation in tetrapods will be discussed in the light of recent developments.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 3","pages":"102-26"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12472979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The vasopressin positive elements in the hypothalamic magnocellular nuclei of rats treated with histamine. 组胺处理大鼠下丘脑大细胞核抗利尿激素阳性成分。
Biological structures and morphogenesis Pub Date : 1992-01-01
A López-Muñiz, I Cuesta-Fernández, J De Miguel
{"title":"The vasopressin positive elements in the hypothalamic magnocellular nuclei of rats treated with histamine.","authors":"A López-Muñiz,&nbsp;I Cuesta-Fernández,&nbsp;J De Miguel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The vasopressin (AVP) positive elements of the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei, were investigated in 180 male rats through immunohistochemical, morphometric and statistical methods. The rats were subdivided in the next groups: 20 control rats; 40 rats treated with physiological saline intraperitoneal via (ip); 40 rats treated with physiological saline intracerebroventricular via (icv); 40 histamine (HA) treated rats, ip; and 40 HA treated rats, icv. The labeled nerve cells appear in the lateral part of the PNV and the SON of the control animals. These neurons have central nucleus and vasopressin positive cytoplasmic granulations. After the treatment with physiological saline, ip or icv, no alterations were observed. In HA treated rats, icv, numerous neurons strongly labeled were observed in these hypothalamic nuclei. Vasopressin positive nerve fibers and large droplets were also found both in the SON and in the PVN of these animals. The vasopressin positive material in the control rats and in HA treated rats, ip, is similar. The morphometric and statistical studies confirm these findings. The results are discussed in this paper.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 4","pages":"153-60"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12482803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of chick or quail embryonic testes on the reproductive system of the quail embryo. 鸡或鹌鹑胚胎睾丸对鹌鹑胚胎生殖系统的影响。
Biological structures and morphogenesis Pub Date : 1992-01-01
T Rogulska, A G Klotz, J Laszczych-Lupinska
{"title":"Influence of chick or quail embryonic testes on the reproductive system of the quail embryo.","authors":"T Rogulska,&nbsp;A G Klotz,&nbsp;J Laszczych-Lupinska","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reproductive system of female quail embryo undergoes masculinization under the influence of chick and quail embryonic testis grafted into an extraembryonic coelom. Müllerian ducts undergo regression. The left gonad transforms into an ovotestis and the right gonad either does not develop or transforms into a small testis. Testicular chick or quail graft does not affect the development of the cortical ovarian-like region in the left testis of male hosts. In some quail testicular grafts developing in female quail hosts cortical region is retained longer than normally.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 1","pages":"23-7"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12591836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The hare parotid gland: ultrastructure and histochemistry of acinar and ductal cells. 兔腮腺腺泡和导管细胞的超微结构和组织化学。
Biological structures and morphogenesis Pub Date : 1992-01-01
A M Bondi, G Menghi, L Marchetti
{"title":"The hare parotid gland: ultrastructure and histochemistry of acinar and ductal cells.","authors":"A M Bondi,&nbsp;G Menghi,&nbsp;L Marchetti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The parotid gland of the hare Lepus europaeus was studied by electron microscopy. The parenchyma consists of secretory areas (acini) and ductal segments (intercalated, striated and excretory ducts). Acinar cells showed a marked heterogeneity due to the presence of secretory granules characterized by different size, electron-density and degree of membrane merging in addition to the occurrence of flattened and dilated rough endoplasmic reticulum. Acinar cells had variable affinity for PAS and PA-TCH-SP staining but were HID- and LIT-TCH-SP negative. Intercalated duct cells lacked secretory granules. Striated ducts were lined by four cell types namely light cells, dark cells, vacuolated cells, and typical striated cells. Excretory ducts showed some scalloping on the apical region of the light cells.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 3","pages":"69-77"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12458621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization of the nuclear receptors of triiodothyronine (T3) by immunocytochemistry under electron microscopy. 三碘甲状腺原氨酸(T3)核受体的电镜免疫细胞化学表征。
Biological structures and morphogenesis Pub Date : 1992-01-01
A Blanco, J Mallol, M Rodriguez, A Montero
{"title":"Characterization of the nuclear receptors of triiodothyronine (T3) by immunocytochemistry under electron microscopy.","authors":"A Blanco,&nbsp;J Mallol,&nbsp;M Rodriguez,&nbsp;A Montero","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>By immunocytochemical methods, using colloidal gold particles coupled to antibodies, it is possible to label the occupied nuclear T3 receptors and observe them by electron microscopy. We carried out some experiments to validate these methods by testing the absence of non-specific binding of the antibodies to subcellular structures different from the T3 receptors. So, we have verified that the number of occupied T3 receptors in tissues from normal rats is lower in kidney cells than in heart and liver ones, a finding which is an agreement with data reported by others. The occupation of the receptors increases after T3 administration and decreases by triiodothyroacetic acid (Triac) administration to the rats, due to the displacement of T3 from the receptors with the latter. Our results are similar to those of the other authors using radioactive tracers. Non-specific binding was not detected. The occupied T3 receptors labelled with colloidal gold particles are located most commonly in the hetero/euchromatin interface. From our results we conclude that the colloidal gold particles observed by electron microscopy specifically label occupied T3 receptors, and the determination of the thyroid status at cellular level can be achieved using electron microscopy after labelling the receptors by immunocytochemistry with colloidal gold particles coupled to antibodies.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 1","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12591837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatial arrangement of the interpositorubral projection in the rabbit. A retrograde HRP study. 兔脑间投影的空间排列。逆行HRP研究。
Biological structures and morphogenesis Pub Date : 1992-01-01
A Ostrowska, L Zguczynski, R Zimny
{"title":"Spatial arrangement of the interpositorubral projection in the rabbit. A retrograde HRP study.","authors":"A Ostrowska,&nbsp;L Zguczynski,&nbsp;R Zimny","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The objective of this investigation was to analyse the organization of rubral afferents from the cerebellar interpositus nuclear complex (NI) in the rabbit. Free HRP was employed as a retrograde tracer to identify the distribution patterns of labeled cells in the anterior and posterior interpositus nuclei. The interpositus projections distribute throughout nearly the entire contralateral red nucleus (RN) except for its rostralmost parvocellular region (RN-PA) which does not correspond to the usually distinguished parvocellular subdivision of the red nucleus (RN-P). The central and lateral regions of the posterior interpositus nucleus (NIP) project (nearly exclusively) to the medial region of the RN. The lateral region of the anterior interpositus nucleus (NIA) sends projections to both the lateral and medial regions of the caudal three-quarters of the RN. Thus, the lateral region of the RN appears to be under nearly exclusive control of NIA. The rostral one quarter of the RN receives cerebellar afferents from the NIP only and caudal pole of RN from the NIA exclusively. Comparison of labelling patterns indicates absence of topogrophic differentiation of projections from the NIP, whereas dorsoventral topography in the NIA can correlate to the rostrocaudal and mediolateral arrangement of the RN. Collectively, interpositorubral projections in the rabbit are similar to those in other species only in basic features.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 4","pages":"129-43"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12482801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Permeability-related structures in developing and mature microvessels of the chicken optic tectum. 鸡视神经顶盖发育和成熟微血管的通透性相关结构。
Biological structures and morphogenesis Pub Date : 1992-01-01
M Bertossi, L Mancini, A Favia, B Nico, D Ribatti, D Virgintino, L Roncali
{"title":"Permeability-related structures in developing and mature microvessels of the chicken optic tectum.","authors":"M Bertossi,&nbsp;L Mancini,&nbsp;A Favia,&nbsp;B Nico,&nbsp;D Ribatti,&nbsp;D Virgintino,&nbsp;L Roncali","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The mode and the temporal sequence of the modifications undergone by permeability-related structures in the neural microvessels have been ultrastructurally and morphometrically investigated in optic tecta of 6, 14, and 18 incubation day (i.d.) chicken embryos and of 30 day chickens. Horseradish peroxidase was utilized as a permeability marker. The endo- and exocytosis-related structures (vesicles and vacuoles) and the interendothelial junctions remarkably change during development: the density of the vacuoles is decreased at the 14th i.d., while that of the vesicles becomes significantly low at the 18th i.d., both reaching lowest values in the chicken; the passage of the marker through the endothelial junctions begins to be hindered from the 14th i.d., parallel to the perivascular arrangement of astrocytic glia endfeet, and it is completely blocked at hatching time. The findings suggest that the optic tectum microvessels are permeable, and thus immature, in the early development and progressively acquire morphofunctional features of vessels provided with barrier devices during the pre- and post-natal development of the brain.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 4","pages":"144-52"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12482802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The oxytocin positive elements in the hypothalamic magnocellular nuclei of rats treated with histamine. 组胺处理大鼠下丘脑大细胞核中催产素阳性成分。
Biological structures and morphogenesis Pub Date : 1992-01-01
A López-Muñiz, I Cuesta Fernández, J de Miguel, E Bengoechea, A Pérez-Casas
{"title":"The oxytocin positive elements in the hypothalamic magnocellular nuclei of rats treated with histamine.","authors":"A López-Muñiz,&nbsp;I Cuesta Fernández,&nbsp;J de Miguel,&nbsp;E Bengoechea,&nbsp;A Pérez-Casas","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The oxytocin positive elements of the hypothalamic supraoptic (SON) and paraventricular (PVN) nuclei, were instigated in 160 male rats through immunohistochemical, morphometric and statistical methods. The rats subdivided into five groups: 20 control rats; 20 rats treated with physiological saline intraperitoneal via (ip); 20 rats treated with physiological saline intracerebroventricular via (icv); 20 histamine (HA) treated rats, ip; and 20 HA treated rats, icv. Some labeled nerve cells appear, mainly, in the lateral part of the SON and the PVN of the control animals. These neurons have central nucleus and oxytocin positive cytoplasmic granulations. After the treatment with physiological saline, ip or icv, no alterations were observed. In HA treated rats, icv, numerous neurons and pathways were observed strongly labeled. Large droplets appear in the SON and in the PVN of these animals. In HA treated rats, ip, the oxytocin positive material is similar to that observed in the control rats. The morphometric and statistical studies confirm these findings. The results are discussed in this paper.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 1","pages":"16-22"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12591349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Morphometric analysis of sciatic nerve and its main branches in the rabbit. 兔坐骨神经及其主要分支的形态计量学分析。
Biological structures and morphogenesis Pub Date : 1992-01-01
G Germanà, U Muglia, M Santoro, F Abbate, R Laurà, M A Gugliotta, G Vita, E Ciriaco
{"title":"Morphometric analysis of sciatic nerve and its main branches in the rabbit.","authors":"G Germanà,&nbsp;U Muglia,&nbsp;M Santoro,&nbsp;F Abbate,&nbsp;R Laurà,&nbsp;M A Gugliotta,&nbsp;G Vita,&nbsp;E Ciriaco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the rabbit the myelinated fibres with the largest diameter originating from the sciatic nerve distribute mainly into the peroneal nerve, even though there is no difference in the density of myelinated fibres of the representative unit between the sciatic nerve and its branches. The distribution of the myelinated fibre diameters is unimodal in the sciatic nerve, but bimodal both in the peroneal and tibial nerves. The quotient axon diameter/fiber diameter (g ratio) shows a different trend between the sciatic nerve and the branches originating from it. Except for the peroneal nerve, the increase of the axon calibre corresponds to the reduction of the relative myelin sheath thickness and therefore to the reduction of internodal distance. The results show that the sciatic nerve and its terminal branches in the rabbit have morphometrical characteristics that are peculiar and differ from those of the other rodents.</p>","PeriodicalId":77037,"journal":{"name":"Biological structures and morphogenesis","volume":"4 1","pages":"11-5"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12591348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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