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Acta chemica Scandinavica (Copenhagen, Denmark : 1989)最新文献

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Immunochromatographic assay for quantitation of milk progesterone. 乳中黄体酮的免疫层析定量分析。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1996-02-01 DOI: 10.3891/acta.chem.scand.50-0141
M P Laitinen, M Vuento
{"title":"Immunochromatographic assay for quantitation of milk progesterone.","authors":"M P Laitinen,&nbsp;M Vuento","doi":"10.3891/acta.chem.scand.50-0141","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.50-0141","url":null,"abstract":"<p><p>We describe a rapid immunochromatographic method for the quantitation of progesterone in bovine milk. The method is based on a 'competitive' assay format using the monoclonal antibody to progesterone and a progesterone-protein conjugate labelled with colloidal gold particles. The monoclonal antibody to progesterone is immobilized as a narrow detection zone on a porous membrane. The sample is mixed with colloidal gold particles coated with progesterone-protein conjugate, and the mixture is allowed to migrate past the detection zone. Migration is facilitated by capillary forces. The amount of labelled progesterone-protein conjugate bound to the detection zone, as detected by photometric scanning, is inversely proportional to the amount of progesterone present in the sample. Analysis is complete in less than 10 min. The method has a practical detection limit of 5 ng of progesterone per ml of bovine milk.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"50 2","pages":"141-5"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19787616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 45
Separation of proteins of nearly the same size but having different isoelectric points by convective electrophoresis. 用对流电泳法分离大小几乎相同但具有不同等电点的蛋白质。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1996-02-01 DOI: 10.3891/acta.chem.scand.50-0102
A K Kontturi, K Kontturi, M Vuoristo
{"title":"Separation of proteins of nearly the same size but having different isoelectric points by convective electrophoresis.","authors":"A K Kontturi,&nbsp;K Kontturi,&nbsp;M Vuoristo","doi":"10.3891/acta.chem.scand.50-0102","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.50-0102","url":null,"abstract":"<p><p>A method for separating proteins of nearly the same size but having different isoelectric points is presented. The method used is convective electrophoresis, which is based on the simultaneous effects of diffusion, electrical migration and opposing convective flow on the transport of proteins through a porous membrane. The pairs of model proteins used in this study were cytochrome c-lysozyme and human serum albumin-haemoglobin. The obtained results show that with a careful adjustment of pH these proteins can be separated by a continuous manner, and no coupling of protein fluxes could be detected in either case.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"50 2","pages":"102-6"},"PeriodicalIF":0.0,"publicationDate":"1996-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19788330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Synthesis of p-phosphonomethyl-L-phenylalanine using camphor sultam or D-valine as chiral auxiliaries and its incorporation into integrin sequences. 以樟脑素坦或d -缬氨酸为手性助剂合成对膦甲基- l-苯丙氨酸及其整合到整合素序列中。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1996-01-01 DOI: 10.3891/acta.chem.scand.50-0054
E Larsson, B Lüning
{"title":"Synthesis of p-phosphonomethyl-L-phenylalanine using camphor sultam or D-valine as chiral auxiliaries and its incorporation into integrin sequences.","authors":"E Larsson,&nbsp;B Lüning","doi":"10.3891/acta.chem.scand.50-0054","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.50-0054","url":null,"abstract":"<p><p>The synthesis of N-Boc-p-phosphonomethyl-L-phenylalanine with two different chiral auxiliaries, camphor sultam or D-valine is described. The preparations have essentially identical properties and have been used to incorporate the amino acid into two integrin peptides as non-hydrolyzable isosteres of phosphotyrosine.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"50 1","pages":"54-7"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19612378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Ganglioside extraction from erythrocytes: a comparison study. 从红细胞中提取神经节苷脂的比较研究。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-12-01 DOI: 10.3891/acta.chem.scand.49-0929
W Q Wang, A Gustafson
{"title":"Ganglioside extraction from erythrocytes: a comparison study.","authors":"W Q Wang,&nbsp;A Gustafson","doi":"10.3891/acta.chem.scand.49-0929","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0929","url":null,"abstract":"<p><p>Several methods for ganglioside extraction from erythrocytes have been compared. Our results show that ganglioside extraction is unfavourably affected by the addition of the solvents as a mixture and by the use of less polar solvents and by a lower total solvent-to-sample ratio. In accordance with our previous observation on cholesterol and phospholipids, the distribution of gangliosides could be uneven in an apparently monophasic extraction solvent mixture. The uneven distribution occurred during and also after the extraction (in filtration and centrifugation). In the recommended method using 19 volumes of methanol/chloroform (2:1) solvent in a one-step extraction, the above disadvantages in ganglioside extraction and quantification are kept under control. This method appears simple and it gives a high recovery of gangliosides.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 12","pages":"929-36"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19515138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Synthesis, analysis and toxicity of three compounds formed during the synthesis of iodixanol. 碘二醇合成过程中形成的三种化合物的合成、分析及毒性。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-10-01 DOI: 10.3891/acta.chem.scand.49-0737
H Priebe, H Dugstad, I F Heglund, R Sande, C P Tønseth
{"title":"Synthesis, analysis and toxicity of three compounds formed during the synthesis of iodixanol.","authors":"H Priebe,&nbsp;H Dugstad,&nbsp;I F Heglund,&nbsp;R Sande,&nbsp;C P Tønseth","doi":"10.3891/acta.chem.scand.49-0737","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0737","url":null,"abstract":"<p><p>The origin of 4-acetyl-2-[N-acetyl-3,5-bis(2,3-dihydroxypropylcarbamoyl)- 2,4,6-triiodoanilinomethyl]-5,7-diiodo-3,4-dihydro-2H-benzo[1,4]ox azine- 6,8-dicarboxylic acid bis(2,3-dihydroxypropylamide) = N-acetyl cyclized iodixanol, 2-[N-acetyl-3,5-bis-(2,3-dihydroxypropylcarbamoyl)-2,4,6- triiodoanilinomethyl]-5,7-diiodo-3,4-dihydro-2H-benzo[1,4]oxazine- 6,8-dicarboxylic acid bis(2,3-dihydroxypropylamide) = cyclized iodixanol and 5,5'-(N-acetyl-2-hydroxypropane-1,3-diyldiamino)bis[N,N'-bis(2,3- dihydroxypropyl)-2,4,6-triiodoisophthalamide] = deacetyl iodixanol in the manufacturing process of the X-ray contrast agent 3,3',5,5'-tetrakis(2,3-dihydroxypropylcarbamoyl)-2,2',4,4',6,6'-++ +hexaiodo- N,N'-(2-hydroxypropane-1,3-diyl)diacetanilide = iodixanol is discussed and their synthesis and purification are described. Their physical and toxicological properties, and analytical and spectroscopic data are summarized.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 10","pages":"737-43"},"PeriodicalIF":0.0,"publicationDate":"1995-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18582822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Synthesis of an N-methyldehydroalanine-containing fragment of microcystin by combination of solid phase peptide synthesis and beta-elimination in solution. 固相多肽合成与液相消除相结合合成含n -甲基去氢丙氨酸的微囊藻毒素片段。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-09-01 DOI: 10.3891/acta.chem.scand.49-0696
M Zetterström, L Trogen, L G Hammarström, L Juhlin, B Nilsson, C Damberg, T Bartfai, U Langel
{"title":"Synthesis of an N-methyldehydroalanine-containing fragment of microcystin by combination of solid phase peptide synthesis and beta-elimination in solution.","authors":"M Zetterström,&nbsp;L Trogen,&nbsp;L G Hammarström,&nbsp;L Juhlin,&nbsp;B Nilsson,&nbsp;C Damberg,&nbsp;T Bartfai,&nbsp;U Langel","doi":"10.3891/acta.chem.scand.49-0696","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0696","url":null,"abstract":"<p><p>A new method for the synthesis of dehydroalanine (delta Ala)-containing peptides has been developed by combining solid phase peptide synthesis (tert-butyloxycarbonyl/HF-chemistry) with solution synthesis. A sequence from cyanobacterial hepatotoxin microcystin, Ac-D-gamma-Glu-[N-Me-delta Ala]-D-Ala-Leu amide was chosen as a model peptide. The precursor for the synthesis of the dehydroalanine-containing peptide, Ac-D-gamma-Glu-[N,S-diMeCys]-D-Ala-Leu, was synthesized on a solid phase followed by sulfonium salt formation on the resin. The resulting S,S-dimethylated peptide was cleaved from the resin with liquid HF. The HPLC-purified S,S-dimethylated cysteine-containing precursor peptide was subjected to beta-elimination in solution catalysed by DBU (1,8-diazabicyclo[5.4.0]undec-7-ene) in methanol. The final product, Ac-D-gamma-Glu-[N-Me-delta Ala]-D-Ala-Leu amide, was purified by HPLC, and analysed by mass spectrometry and 1H NMR spectroscopy. The stability of the model peptide under acidic, neutral and basic conditions has been studied.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 9","pages":"696-700"},"PeriodicalIF":0.0,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18554414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Synthesis of 3'-O-(2-aminoethyl)-2'-deoxyuridines. 3'- o -(2-氨基乙基)-2'-脱氧尿苷的合成。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-08-01 DOI: 10.3891/acta.chem.scand.49-0609
A A Abdel Aleem, E Larsen, E B Pedersen, C Nielsen
{"title":"Synthesis of 3'-O-(2-aminoethyl)-2'-deoxyuridines.","authors":"A A Abdel Aleem,&nbsp;E Larsen,&nbsp;E B Pedersen,&nbsp;C Nielsen","doi":"10.3891/acta.chem.scand.49-0609","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0609","url":null,"abstract":"<p><p>Methyl 2-deoxy-3-O-[2-(formylamino)ethyl]-5-O-trityl-D-erythro- pentofuranoside (4) was obtained in a 3-O alkylation reaction by treatment with 2-chloroethylamine in DMF. Compound 4 afforded alpha nucleosides as the main products when condensed with uracils under the Vorbrüggen conditions. The nucleosides were deblocked by treatment with 80% acetic acid and subsequently with sodium methoxide in methanol.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 8","pages":"609-14"},"PeriodicalIF":0.0,"publicationDate":"1995-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18665539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Sequence-selective metal ion binding to DNA oligomers. 序列选择性金属离子结合DNA低聚物。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-07-01 DOI: 10.3891/acta.chem.scand.49-0495
S Steinkopf, A Garoufis, W Nerdal, E Sletten
{"title":"Sequence-selective metal ion binding to DNA oligomers.","authors":"S Steinkopf,&nbsp;A Garoufis,&nbsp;W Nerdal,&nbsp;E Sletten","doi":"10.3891/acta.chem.scand.49-0495","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0495","url":null,"abstract":"<p><p>Sequence-selective interactions between DNA oligonucleotides and Pt(II) and Pd(II) complexes have been studied by 1D and 2D NMR spectroscopy. Titration of DNA oligomers with diamagnetic metal ion complexes induces chemical shifts of proton resonances close to the site of interaction. Conformational changes in the helical structure were monitored by measuring cross-peak intensities in 2D NOESY maps. Two duplex deoxynucleotides were studied, [d(CGCGCG)]2 and [d(CGCGAATTCGCG)]2, respectively. The hexamer was titrated in the duplex form with cis-[Pt(NH3)2Cl2]2+ (cis-DDP or cisplatin). The dodecamer was titrated with NO(3-)-salts of Pd(aq)2+, [Pd(en)(H2O)2]2+ and [Pd(dien)(H2O)]+, respectively. The reaction between cis-DDP and the hexamer at conditions where the duplex form is retained proceeded exceedingly slowly. In the initial phase of the titration the NOESY map indicates conformational changes induced by noncovalent adduct formation between the intact hexamer and cis-DDP. The more reactive Pd compounds show a tendency towards sequence-selective binding to the duplex dodecamer. The 'naked' Pd ion is very reactive and exhibits selectivity towards the thymine T8 imino proton and N7 on G4. At a Pd(II)/dodecamer ratio of 4:1 the metal ions induce helix-->coil transition. The mono- and bifunctional Pd complexes with 'bulky' ligands attack the dodecamer at the terminal GC base pairs, leaving the central Watson-Crick base pairs intact.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 7","pages":"495-502"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18625026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure-activity relationships for unsaturated dialdehydes. 10. The generation of bioactive products by autoxidation of isovelleral and merulidial. 不饱和二醛的构效关系。10. 异柳醛和多柳醛自氧化生成生物活性产物。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-07-01 DOI: 10.3891/acta.chem.scand.49-0530
M Jonassohn, H Anke, P Morales, O Sterner
{"title":"Structure-activity relationships for unsaturated dialdehydes. 10. The generation of bioactive products by autoxidation of isovelleral and merulidial.","authors":"M Jonassohn,&nbsp;H Anke,&nbsp;P Morales,&nbsp;O Sterner","doi":"10.3891/acta.chem.scand.49-0530","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0530","url":null,"abstract":"<p><p>The autoxidation of the two mutagenic and antimicrobial sesquiterpenes merulidial (1a) and isovelleral (5) generates a number of bioactive products that are suggested to be partly responsible for the mutagenic and antimicrobial activities of the two compounds. The biological activities of the autoxidation products are, as far as has been possible to assay, of the same order as the parent compounds, and it is shown that they are formed in normal bioassay media. Merulidial (1a) is especially interesting, as its rate of autoxidation is considerably higher compared with non-mutagenic derivatives of 1a. In addition, natural isovelleral [(+/-)-5] was found to be twice as mutagenic as synthetic isovelleral [(+/-)-5], indicating that (-)-isovelleral is only weakly active or inactive, and supporting the suggestion that the interaction of isovelleral (5) and its derivatives with DNA depends on the absolute stereochemistry of the unsaturated dialdehyde moiety.</p>","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 7","pages":"530-5"},"PeriodicalIF":0.0,"publicationDate":"1995-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18623617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
Synthesis of ethylmorphine-6-glucuronide: a metabolite of ethylmorphine in man. 乙基吗啡-6-葡萄糖醛酸盐的合成:人体内乙基吗啡的代谢物。
Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1995-05-01 DOI: 10.3891/acta.chem.scand.49-0380
A Bugge, T Aasmundstad, A J Aasen, A S Christophersen, S Morgenlie, J Mørland
{"title":"Synthesis of ethylmorphine-6-glucuronide: a metabolite of ethylmorphine in man.","authors":"A Bugge,&nbsp;T Aasmundstad,&nbsp;A J Aasen,&nbsp;A S Christophersen,&nbsp;S Morgenlie,&nbsp;J Mørland","doi":"10.3891/acta.chem.scand.49-0380","DOIUrl":"https://doi.org/10.3891/acta.chem.scand.49-0380","url":null,"abstract":"","PeriodicalId":76966,"journal":{"name":"Acta chemica Scandinavica (Copenhagen, Denmark : 1989)","volume":"49 5","pages":"380-4"},"PeriodicalIF":0.0,"publicationDate":"1995-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"18766802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
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