Separation of proteins of nearly the same size but having different isoelectric points by convective electrophoresis.

A K Kontturi, K Kontturi, M Vuoristo
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引用次数: 3

Abstract

A method for separating proteins of nearly the same size but having different isoelectric points is presented. The method used is convective electrophoresis, which is based on the simultaneous effects of diffusion, electrical migration and opposing convective flow on the transport of proteins through a porous membrane. The pairs of model proteins used in this study were cytochrome c-lysozyme and human serum albumin-haemoglobin. The obtained results show that with a careful adjustment of pH these proteins can be separated by a continuous manner, and no coupling of protein fluxes could be detected in either case.

用对流电泳法分离大小几乎相同但具有不同等电点的蛋白质。
提出了一种分离大小几乎相同但具有不同等电点的蛋白质的方法。所使用的方法是对流电泳,它是基于扩散,电迁移和反向对流对蛋白质通过多孔膜运输的同时影响。本研究使用的模型蛋白对是细胞色素c-溶菌酶和人血清白蛋白-血红蛋白。结果表明,通过仔细调整pH值,这些蛋白质可以连续分离,并且在两种情况下都没有检测到蛋白质通量的偶联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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