Voprosy biokhimii mozga最新文献_第8页

[Effect of cations and DNP on ATPase activity in different tissue fractions of birds' brains]. [阳离子和DNP对鸟类脑组织不同组织部分atp酶活性的影响]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

A A Simonian, R A Stepanian, G G Batikian

{"title":"[Effect of cations and DNP on ATPase activity in different tissue fractions of birds' brains].","authors":"A A Simonian, R A Stepanian, G G Batikian","doi":"","DOIUrl":"","url":null,"abstract":"ATP-ase activity and the effect of cations (Na+, K+, Mg2+, Ca2+) and DNP on its activity have been studied in avian brain homogenates and mitochondrial and supernatant fractions (microsomes + hyaloplasma). Highest levels of Na+ + K+ -stimulated ATP-ase activity has been shown in avian brain crude mitochondrial fraction, next in supernatant and lowest in homogenates. Following three-fold freezing and thawing, enzyme activity in homogenates and mitochondrial fractions increases considerably and decreases in the supernatant. Highest Mg2+ -stimulated ATP-ase activity is found in avian brain homogenates, next in mitochondrial fractions and supernatants. Mg2+ -stimulated ATP-ase activity is not changed considerably following three-fold freezing and thawing of brain preparations. Highest Ca2+ -stimulated ATP-ase activity has been observed in the supernatant fraction and its activity is considerably reduced following freezing and thawing. Avian brain mitochondrial fractions have high DNP-stimulated ATP-ase activity. Following freezing and thawing of brain preparations DNP-stimulated ATP-ase activity is not changed considerably. The results obtained indicate that in avian brain homogenates and other preparations, cation and DNP-stimulated ATP-ase have different activities. Three-fold freezing and thawing of brain tissue preparations affect these activities differentially.","PeriodicalId":76813,"journal":{"name":"Voprosy biokhimii mozga","volume":"9 ","pages":"219-26"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15389938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Changes in the terminal oxidation and active transport of calcium ions in the cerebral mitochondria of white rats following chronic cerebroside administration]. [慢性脑苷给药后大鼠脑线粒体钙离子终末氧化和主动转运的变化]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

E E Mkheian, O P Sotskiĭ, E S Sekoian

引用次数: 0

[Ribonuclease and DNA-dependent-RNA-polymerase activity in the brain under normal physiologic conditions]. [正常生理条件下脑内核糖核酸酶和dna依赖性rna聚合酶活性]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

G S Khachatrian, A A Antonian, A A Alaverdian, F A Sarkisian

{"title":"[Ribonuclease and DNA-dependent-RNA-polymerase activity in the brain under normal physiologic conditions].","authors":"G S Khachatrian, A A Antonian, A A Alaverdian, F A Sarkisian","doi":"","DOIUrl":"","url":null,"abstract":"Ribonuclease activity of nuclear, ribosomal and soluble fractions and DNA linked RNA polymerase activity of rat brain has been studied following alimentary, conditioned alimentary stimulation and conditioned alimentary inhibition. During alimentary and conditioned alimentary stimulation ribonuclease activity is considerably reduced at pH 5.4; 6.0; 7.9 and 8.1 but not at pH 7.6. Under these same conditions ribonuclease activity is high in soluble fraction. DNA-linked RNA-polymerase activity increases during stimulation. During conditioned alimentary inhibition ribonuclease activity of various subcellular fractions is also reduced. Ribonuclease activity of soluble fraction is lower than that observed during stimulation. During inhibition DNA-dependent RNA-polymerase activity is also significantly raised. The significant reduction of ribonuclease activity of nuclear and ribosomal fractions and the significant increase of DNA-dependent RNA-polymerase activity in the nuclear fraction during stimulation and inhibition of brain activity indicate the importance of RNA-polymerase and the biosynthesis of different kinds of RNA during activity of higher brain centers.","PeriodicalId":76813,"journal":{"name":"Voprosy biokhimii mozga","volume":"9 ","pages":"151-70"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15571985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Glutamine synthetase and glutaminase activity in the brain and liver homogenates and subcellular fractions of rats with alloxan diabetes (3)]. [四氧嘧啶型糖尿病大鼠大脑和肝脏匀浆和亚细胞部分谷氨酰胺合成酶和谷氨酰胺酶活性[3]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

G K Buniatian, V B Egian, G A Turshian, G E Akopian, S S Safrazian

{"title":"[Glutamine synthetase and glutaminase activity in the brain and liver homogenates and subcellular fractions of rats with alloxan diabetes (3)].","authors":"G K Buniatian, V B Egian, G A Turshian, G E Akopian, S S Safrazian","doi":"","DOIUrl":"","url":null,"abstract":"Glutaminsynthetase activity has been determined in microsomal and soluble fractions of brain and liver of normal and alloxan diabetic rats and glutaminase activity in homogenates and mitochondrial fractions. The results obtained indicate that in brain of diabetic animals glutaminesynthetase activity of microsomal fractions is changed very slightly while that of the soluble fraction is marked reduced. In liver tissue enzyme activity is significantly decreased in both microsomal and soluble fractions. In homogenates of brain tissue of diabetic animals glutaminase does not differ markedly from that of control animals both in the presence and absence of activators (phosphate, aspartate, bicarbonate). In homogenates of liver, in the absence of activators, glutaminase activity is greater in diabetic animals than in controls, it is not much changed in the presence of phosphate and decreases in the presence of bicarbonate and aspartate. In diabetic animals, in the presence of phosphate, glutaminase activity of brain mitochondrial fractions increases while that of liver mitochondrial fractions does not change. It is interesting to note that in the absence of activators glutaminase activity is absent in both brain and liver mitochondria while it is quite high in homogenates.","PeriodicalId":76813,"journal":{"name":"Voprosy biokhimii mozga","volume":"9 ","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15267316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Intracellular influence of different mediators on acid ribonuclease activity in brain tissue]. 不同介质对脑组织酸性核糖核酸酶活性的胞内影响。

Voprosy biokhimii mozga Pub Date : 1974-01-01

N N Demin, G A Nechaeva

引用次数: 0

[Phospholipid composition of the proteolipids of the white matter of different brain and spinal cord regions and of the sciatic nerve in dogs]. [犬不同脑、脊髓区域和坐骨神经白质的磷脂组成]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

K G Manukian, G K Buniatian

引用次数: 0

[Quantitative changes in the protein of proteolipids in different portions of the nervous system and in the heart during rat ontogenesis]. [大鼠个体发育过程中神经系统不同部位和心脏蛋白脂质的定量变化]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

K G Manukian, L G Kirakosian, K L Levonian, V N Zakharian

引用次数: 0

[Effect of thiol reagents on cerebral glutaminase activity]. [硫醇试剂对大脑谷氨酰胺酶活性的影响]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

V S Oganesian, G K Buniatian, K S Mikirtumova, R L Airapetian

{"title":"[Effect of thiol reagents on cerebral glutaminase activity].","authors":"V S Oganesian, G K Buniatian, K S Mikirtumova, R L Airapetian","doi":"","DOIUrl":"","url":null,"abstract":"It has been shown that the glutaminase activity of brain mitochondrial fractions, which is stimulated by various activators, is completely abolished on 10 min preincubation with parachlormercurybenzoate, 5,5'-dithiobis-(2-nitrobenzoate), N-ethylmaleimide, iodoacetamid, CdCl2, ZnCl2 and CuCl2. With the purpose of elucidating the role of SH-groups in glutaminase activity we have studied the possibilities of preventing loss of enzyme activity by substrates and activators. We have shown that glutamine is effective only in the case of CuCl2. It should be noted that preincubation of brain mitochondrial fractions with activators greatly reduces the inhibitory effects of thiol reagents. Besides, activators differ in their effect in preventing glutaminase inactivation by various SH-reagents. After preincubation of glutaminase with activators their protective effect depends on whether glutamin is added together with the inhibitor or after some time. In one case their protective effect increases, in the other decreases and in the third case remains unchanged. On the basis of the data obtained the authors conclude that SH groups of brain glutaminase are not present in the active center and have an important functional significance for its catalytic active conformation.","PeriodicalId":76813,"journal":{"name":"Voprosy biokhimii mozga","volume":"9 ","pages":"5-16"},"PeriodicalIF":0.0,"publicationDate":"1974-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"15576180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of gamma-aminobutyric acid on the concentration of noradrenaline in the synaptosoma fraction of the meso-diencephalic region of rat brain]. [γ -氨基丁酸对大鼠脑中间脑区突触体部分去甲肾上腺素浓度的影响]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

L N Arakelian, A A Demirchian, A M Petrosian, E K Kazarova, N A Esaian

引用次数: 0

[Changes in the activities of NAD- and NADP-specific isocitrate dehydrogenases in the brain and liver during the postembryonic development of animals]. [动物胚胎后发育过程中脑和肝脏中NAD和nadp特异性异柠檬酸脱氢酶活性的变化]。

Voprosy biokhimii mozga Pub Date : 1974-01-01

M I Prokhorova, F E Putilina, N D Eshchenko

引用次数: 0