[Effect of cations and DNP on ATPase activity in different tissue fractions of birds' brains].

Voprosy biokhimii mozga Pub Date : 1974-01-01

A A Simonian, R A Stepanian, G G Batikian

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Abstract

ATP-ase activity and the effect of cations (Na+, K+, Mg2+, Ca2+) and DNP on its activity have been studied in avian brain homogenates and mitochondrial and supernatant fractions (microsomes + hyaloplasma). Highest levels of Na+ + K+ -stimulated ATP-ase activity has been shown in avian brain crude mitochondrial fraction, next in supernatant and lowest in homogenates. Following three-fold freezing and thawing, enzyme activity in homogenates and mitochondrial fractions increases considerably and decreases in the supernatant. Highest Mg2+ -stimulated ATP-ase activity is found in avian brain homogenates, next in mitochondrial fractions and supernatants. Mg2+ -stimulated ATP-ase activity is not changed considerably following three-fold freezing and thawing of brain preparations. Highest Ca2+ -stimulated ATP-ase activity has been observed in the supernatant fraction and its activity is considerably reduced following freezing and thawing. Avian brain mitochondrial fractions have high DNP-stimulated ATP-ase activity. Following freezing and thawing of brain preparations DNP-stimulated ATP-ase activity is not changed considerably. The results obtained indicate that in avian brain homogenates and other preparations, cation and DNP-stimulated ATP-ase have different activities. Three-fold freezing and thawing of brain tissue preparations affect these activities differentially.

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[阳离子和DNP对鸟类脑组织不同组织部分atp酶活性的影响]。

研究了鸟脑匀浆、线粒体和上清(微粒体+透明体)中atp酶活性以及阳离子(Na+、K+、Mg2+、Ca2+)和DNP对其活性的影响。Na+ + K+刺激的atp酶活性在禽脑粗线粒体中最高，上清次之，匀浆最低。经过三次冷冻和解冻后，匀浆和线粒体组分中的酶活性显著增加，上清中酶活性降低。Mg2+刺激的atp酶活性在禽类脑匀浆中最高，其次是线粒体组分和上清液。Mg2+刺激的atp酶活性在三次冷冻和解冻脑制剂后没有明显变化。在上清部分中观察到最高的Ca2+刺激的atp酶活性，其活性在冷冻和解冻后显着降低。鸟类脑线粒体片段具有高dnp刺激的atp酶活性。在冷冻和解冻脑制剂后，dnp刺激的atp酶活性没有明显变化。结果表明，在禽类脑匀浆及其他制剂中，阳离子和dnp刺激的atp酶具有不同的活性。脑组织三次冷冻和解冻对这些活动的影响是不同的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Voprosy biokhimii mozga

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