[Ribonuclease and DNA-dependent-RNA-polymerase activity in the brain under normal physiologic conditions].

Voprosy biokhimii mozga Pub Date : 1974-01-01

G S Khachatrian, A A Antonian, A A Alaverdian, F A Sarkisian

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Abstract

Ribonuclease activity of nuclear, ribosomal and soluble fractions and DNA linked RNA polymerase activity of rat brain has been studied following alimentary, conditioned alimentary stimulation and conditioned alimentary inhibition. During alimentary and conditioned alimentary stimulation ribonuclease activity is considerably reduced at pH 5.4; 6.0; 7.9 and 8.1 but not at pH 7.6. Under these same conditions ribonuclease activity is high in soluble fraction. DNA-linked RNA-polymerase activity increases during stimulation. During conditioned alimentary inhibition ribonuclease activity of various subcellular fractions is also reduced. Ribonuclease activity of soluble fraction is lower than that observed during stimulation. During inhibition DNA-dependent RNA-polymerase activity is also significantly raised. The significant reduction of ribonuclease activity of nuclear and ribosomal fractions and the significant increase of DNA-dependent RNA-polymerase activity in the nuclear fraction during stimulation and inhibition of brain activity indicate the importance of RNA-polymerase and the biosynthesis of different kinds of RNA during activity of higher brain centers.

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[正常生理条件下脑内核糖核酸酶和dna依赖性rna聚合酶活性]。

研究了大鼠脑在营养刺激、条件营养刺激和条件营养抑制作用下的核、核糖体和可溶性部分的核糖核酸酶活性和DNA连接RNA聚合酶活性。在消化和条件消化刺激期间，核糖核酸酶活性在pH 5.4时显著降低;6.0;7.9和8.1，但不是pH 7.6。在相同条件下，可溶性部分的核糖核酸酶活性高。dna连锁rna聚合酶活性在刺激期间增加。在条件消化抑制期间，各种亚细胞组分的核糖核酸酶活性也降低。可溶性部分的核糖核酸酶活性比刺激时观察到的低。在抑制期间，依赖dna的rna聚合酶活性也显著升高。在脑活动的刺激和抑制过程中，核和核糖体部分的核糖核酸酶活性显著降低，核部分中dna依赖的RNA聚合酶活性显著增加，这表明RNA聚合酶和不同种类RNA的生物合成在脑高级中枢活动中的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Voprosy biokhimii mozga

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