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Observation on drug resistance of Trypanosoma (Nannomonas) congolense and Trypanosoma (Duttonella) vivax in cattle at a feedlot in the Northern Ivory Coast. 科特迪瓦北部某饲养场牛中刚果锥虫(纳米单胞菌)和杜顿氏锥虫(间日疟原虫)的耐药性观察。
Tropenmedizin und Parasitologie Pub Date : 1983-09-01
W Küpper, M Wolters
{"title":"Observation on drug resistance of Trypanosoma (Nannomonas) congolense and Trypanosoma (Duttonella) vivax in cattle at a feedlot in the Northern Ivory Coast.","authors":"W Küpper,&nbsp;M Wolters","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Studies carried out on feedlot cattle show Berenil at 7 mg/kg to be the treatment of choice in trypanosomiasis although repeated doses may be necessary. Resistant strains of Trypanosoma (Duttonella) vivax and Trypanosomas (Nannomonas) congolense survived dosages of between 0.5 mg/kg and 1.0 mg/kg of Trypamidium and were cross resistant to Homidium bromide.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 3","pages":"203-5"},"PeriodicalIF":0.0,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17691422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunocytochemical studies on several developmental stages of Dipetalonema viteae (Filarioidea). 丝状双龙线虫(Dipetalonema viteae)不同发育阶段的免疫细胞化学研究。
Tropenmedizin und Parasitologie Pub Date : 1983-09-01
A Prüsse, S Vollmer, H J Diesfeld
{"title":"Immunocytochemical studies on several developmental stages of Dipetalonema viteae (Filarioidea).","authors":"A Prüsse,&nbsp;S Vollmer,&nbsp;H J Diesfeld","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The binding of antibodies to infective larvae (L3), eggs, uterine contents and blood microfilariae was demonstrated by light microscopy employing the peroxidase-antiperoxidase (PAP) method. Antigen-antibody reactions were located on the shell of filarial eggs as well as on the cuticle and the interior of L3 and microfilariae. Using sera from Meriones unguiculatus, Mastomys natalensis and golden hamsters infected with D. viteae obtained at 7, 14, 21 and 28 weeks p.i. it was observed that the intensity of the immunostaining on several developmental stages of the filariae decreased with increasing duration of infection. This effect was more pronounced in the case of M. unguiculatus and M. natalensis than in the case of golden hamsters. For detection of surface antigenicity the simple procedure of drying the test material on microscopic slides proved to be time saving and equally specific as compared to the embedding in Epon of the filarial stages and the successive preparation of semithin sections. However, embedded filarial stages presented more intensive immunostaining than nonembedded stages. Moreover, sections allowed to demonstrate antigen-antibody reactions on the cuticle as well as inside the body cavity of the stages.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 3","pages":"169-73"},"PeriodicalIF":0.0,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17739841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differentiation of Trypanosoma cruzi Chagas, 1909 and Trypanosoma vespertilionis Battaglia, 1904 by various lectins. 不同凝集素对克氏锥虫、查加斯锥虫(1909)和巴塔利亚锥虫(1904)的分化。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
J Schottelius, O Koch, G Uhlenbruck
{"title":"Differentiation of Trypanosoma cruzi Chagas, 1909 and Trypanosoma vespertilionis Battaglia, 1904 by various lectins.","authors":"J Schottelius,&nbsp;O Koch,&nbsp;G Uhlenbruck","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Four-days-old culture forms of Trypanosoma cruzi (strain Téhuantépéc, Guatemala) and Trypanosoma vespertilionis (strain P-14, P-9) were tested by 19 carbohydrate-specific agglutinins. The T. cruzi strains are interspecifically distinguishable with the lectins from Euonymus europaeus, Tridacna crocea, Tridacna maxima and the human blood-group testserum anti-B from the T. vespertilionis strains. While the T. vespertilionis strains did react with anti-B and E. europaeus, the T. cruzi strains did not agglutinate. The T. cruzi strains were agglutinated by the lectins from T. crocea and T: maxima while the bat-trypanosomes showed no reactions. Using these lectins it was not possible to distinguish the bat-flagellates intraspecifically. With the lectins from Triticum vulgaris and Arachis hypogaea the T. cruzi strains could be distinguished. While the Ténuantépéc strain did agglutinate with A. hypogaea, T. cruzi strain Guatemala did react only with the lectin from T. vulgaris. The bat-trypanosomes were agglutinated only by A. hypogaea but not by T. vulgaris. The reactions of these trypanosome-species with A. papillata and T. vulgaris demonstrate that both trypanosome species have N-acetylneuraminic acid on their cell surfaces.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"89-92"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17408971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macro- and microfilariae in nodules from onchocerciasis patients in the Yemen Arab Republic. 阿拉伯也门共和国盘尾丝虫病患者结核中的大丝虫病和微丝虫病。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
D W Büttner, P Rácz
{"title":"Macro- and microfilariae in nodules from onchocerciasis patients in the Yemen Arab Republic.","authors":"D W Büttner,&nbsp;P Rácz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fourteen subcutaneous nodules from five patients with severe localized onchocerciasis, from one person with a mild dermatitis and from two men with the generalized form of the disease were studied. Ten nodules contained adult parasites with an average sex ratio of 1.6 for female to male worms. The median worm burden was two filariae per person. The onchocercomata of two patients with severe localized onchocerciasis comprised one or two pairs of microfilariae producing worms whereas the microfilaria load in the skin of these patients was estimated to have been less than 100 000 microfilariae. One nodule contained no intact microfilariae but more than 10 000 small granulomas with all stages of degenerating microfilariae whereas the density of 0.08 live microfilariae per milligram in the skin near to the nodule was very low. None of the patients had received antifilarial drugs before nodulectomy. It is concluded, therefore, that these patients with severe localized onchocerciasis possessed the capacity naturally to kill microfilariae. In the nodules of these patients many degenerating microfilariae were observed which were surrounded by eosinophils or macrophages or which lay in small abscesses with neutrophil leucocytes or in small granulomas. The adherence of eosinophil leucocytes to only slightly altered microfilariae could be observed as a first stage.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"113-21"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Acid phosphatase patterns in microfilariae of Onchocerca volvulus s.l. from the Upper Orinoco Basin, Venezuela. 委内瑞拉上奥里诺科河盆地盘尾丝虫微丝的酸性磷酸酶模式。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
L Yarzàbal, I Petralanda, M Arango, L Lobo, C Botto
{"title":"Acid phosphatase patterns in microfilariae of Onchocerca volvulus s.l. from the Upper Orinoco Basin, Venezuela.","authors":"L Yarzàbal,&nbsp;I Petralanda,&nbsp;M Arango,&nbsp;L Lobo,&nbsp;C Botto","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The patterns of acid phosphatase in strains of Onchocerca volvulus s.l. which parasitize an Amerindian population (Yanomami) in Venezuela's Upper Orinoco Basin were examined by using the naphthol AS-TR phosphate method. The study sample consisted of 40 Yanomami inhabiting a savannah area at 950 m above sea level and 21 Yanomami residents of a tropical rainforest area at an altitude of 250 m. Stained intrauterine microfilariae, still within the egg case, exhibited a diffuse distribution of the enzyme in the early stages of embryonic development and a negative reaction at a more developed stage. Four of the five enzyme staining patterns described by Omar (1978) were found in the 3157 microfilariae examined from skin snips. Their distribution was: Type I--17.2%, Type III--0.5%, Type IV--75.6% and Type V--6.6%. No examples of Type II were observed. The results indicate that acid phosphatase patterns of the Upper Orinoco Onchocerca strain most resemble those of strains from Guatemala and Yemen, and are different from the African strains found in Upper Volta and Liberia. The relative frequency of acid phosphatase patterns was modified by cryopreservation of microfilariae.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"109-12"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparison of isoenzymes of some species of the subgenus schizotrypanum from bats by isoelectrofocusing. 等电聚焦法比较蝙蝠裂锥虫亚属几种同工酶。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
F Ebert
{"title":"Comparison of isoenzymes of some species of the subgenus schizotrypanum from bats by isoelectrofocusing.","authors":"F Ebert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Culture forms of bat-trypanosomes of the species T. dionisii, T. vespertilionis and T.c. marinkellei were compared isoenzymatically by isoelectrofocusing. The enzymes tested were: nonspecific esterase (NSE, E.C.3.1.1.), phosphoglucomutase (PGM, E.C. 2.7.5.1), glucose-6-phosphate dehydrogenase (G-6-PD, E.C. 1.1.1.49), glucosephosphate isomerase (GPI, E.C. 5.3.1.9), malate dehydrogenase (MDH, E.C. 1.1.1.37), alcohol dehydrogenase (NADP+) (ADH, E.C. 1.1.1.2). Their enzyme types were related to those of T. cruzi. The comparison of enzyme patterns of the six enzymes has shown that each species was characterized by species-specific enzyme profiles. Among the stocks of the European species, T. dionisii, and T. vespertilionis, variations of the enzyme patterns of PGM, G-6-PD and GPI suggesting that the final status of this subspecies is probably not yet established. In relation to T. cruzi it has been found that T. dionisii showed identical enzyme profiles with group II of T. cruzi. For T. vespertilionis no enzyme types identical with T. cruzi were detectable. T.c. marinkellei showed only identical enzyme patterns to T. cruzi-group I by the enzymes NSE and GPI.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"93-7"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17288268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of immunogenic proteins of Dipetalonema viteae (Filarioidea) by the "Western Blotting" technique. “Western Blotting”技术鉴定viteae双瓣虫免疫原性蛋白(丝状总科)。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
R Lucius, E W Rauterberg, H J Diesfeld
{"title":"Identification of immunogenic proteins of Dipetalonema viteae (Filarioidea) by the \"Western Blotting\" technique.","authors":"R Lucius,&nbsp;E W Rauterberg,&nbsp;H J Diesfeld","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Sodiumdodecylsulphate (SDS)-solubilized proteins of several developmental stages of Dipetalonema viteae (adults, uterus content, microfilariae, infective larvae) were subjected to SDS-polyacrylamide gel electrophoresis and transferred onto nitrocellulose filter sheets. Their antigenicity in natural infections of Meriones unguiculatus was investigated by immuno-recognition with pooled sera of infected hosts. The antibodies were labelled by radioiodinated protein A and visualized by autoradiography. Immunorecognized protein bands were demonstrable in all stages, some of them being stage specific, others common to several or all stages. The highest number of labelled protein bands was found in extracts of adult worms, the lowest in preparations of microfilariae and infective larvae.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"133-6"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17408970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The incidence of lagophthalmus and posterior synechiae of the iris during chemotherapy of leprosy (morbus Hansen). 麻风病化疗期间眼lagmus和虹膜后粘连的发生率。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
F Brandt, P G Kalthoff
{"title":"The incidence of lagophthalmus and posterior synechiae of the iris during chemotherapy of leprosy (morbus Hansen).","authors":"F Brandt,&nbsp;P G Kalthoff","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the Green-Pastures-Leprosy-Hospital in Pikhara/Nepal 340 outpatients with leprosy were examined to evaluate the incidence of lagophthalmus and posterior synechiae of the iris. Patients who had already received chemotherapeutical treatment during the first 5 years following the onset of the disease developed fewer cases of lagophthalmus in both types of leprosy than patients who remained untreated for longer than 5 years. A comparable positive influence of early chemotherapy on the incidence of posterior synechiae of the iris could not be proven. The possible causes were discussed.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"75-8"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Occurrence of Onchocerca lienalis (Stiles, 1892) in cattle in Austria]. [奥地利牛中盘尾丝虫病的发生[Stiles, 1892]。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
N Safar-Hermann, R Supperer
{"title":"[Occurrence of Onchocerca lienalis (Stiles, 1892) in cattle in Austria].","authors":"N Safar-Hermann,&nbsp;R Supperer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The prevalence of O. lienalis in 345 Austrian cattle was determined by detection of microfilariae in the skin. Though only one among 59 male animals yielded a positive result, 46.5% of the female cattle showed to be infected with the parasite. Comparing the presence of adult worms in the gastrosplenic ligament and that of larval stages in the skin a correlation of 87.5% could be obtained. Most of the microfilariae were concentrated in the umbilical region but with severely infested animals they also were found in the skin of the udder, head, ear and neck. Seasonal periodicity of microfilariae was not observed.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"129-32"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significance of hydatid immunodiagnostic surveys to health care and estimation of prevalence in the Argentine Province of Chubut. 阿根廷丘布特省包虫病免疫诊断调查对卫生保健和流行率估计的意义。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
V M Varela-Diaz, E A Guarnera, E A Coltorti, E Angiorama, H Conesa, A Hernández, C Cavallo, R Morrone, R Garcia
{"title":"Significance of hydatid immunodiagnostic surveys to health care and estimation of prevalence in the Argentine Province of Chubut.","authors":"V M Varela-Diaz,&nbsp;E A Guarnera,&nbsp;E A Coltorti,&nbsp;E Angiorama,&nbsp;H Conesa,&nbsp;A Hernández,&nbsp;C Cavallo,&nbsp;R Morrone,&nbsp;R Garcia","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>More hydatidosis cases were detected in an immunodiagnostic survey of rural schoolchildren from an endemic area in Argentina by the arc 5 double diffusion (DD5) test than by indirect hemagglutination (IHA) and latex agglutination (LA) tests. Evidence of infection was obtained by clinical, radiologic, echographic, and/or computerized axial tomography examinations in only one of three DD5-negative individuals, and in 2 of 4 students showing a questionable reaction of identity with arc 5 in DD5, who were positive in LA and/or IHA. In contrast, cysts could be demonstrated in all DD5-positive cases, whether positive or negative in the agglutination procedures. These cysts in DD5-positive cases included the smallest cysts (1-1.2 cm wide) detected in surveys to date. Comparison of data obtained in the immunodiagnostic survey and in a review of hospital records was used to assess the relative contribution of each method in estimating the prevalence of infection. The findings are discussed in terms of the advantages and limitations of carrying out immunodiagnostic surveys of residents of endemic areas for purposes of primary medical care and surveillance.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"98-104"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17469995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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