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[Paragonimus uterobilateralis as the cause of 3 cases of human paragonimiasis in Gabon]. [加蓬3例人类肺吸虫病的病因是子宫两侧吸虫]。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
R Sachs, P Kern, J Voelker
{"title":"[Paragonimus uterobilateralis as the cause of 3 cases of human paragonimiasis in Gabon].","authors":"R Sachs,&nbsp;P Kern,&nbsp;J Voelker","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>During 1981, three cases of pulmonary paragonimiasis were diagnosed and successfully treated at the Albert Schweitzer Hospital, Lambarene, Gabon. Circumstances prevented our receiving eggs for identification of the lung fluke species involved. The history revealed that all the patients originated from the same small village Mitoné, some 10 km north of Lambarene. Extensive testing in the region failed to reveal further cases. Our efforts, therefore, were concentrated on the study of the intermediate crustacean hosts responsible for the human infections. We examined 90 fresh-water crabs of the species Sudanonautes aubryi and S. africanus collected from small creeks located close to the village of the patients. 14 per cent of the former species and 8 per cent of the latter contained freely moving, non-encysted metacercariae resembling those of Paragonimus uterobilateralis. A domestic cat was subsequently infected with these metacercariae. During autopsy of the cat some 260 days later, 2 adult lung flukes were recovered and clearly identified as P. uterobilateralis, confirming the diagnosis on the larval metacercariae. These findings support the surmise that the human cases of paragonimiasis in the same area could be attributed to an infection with P. uterobilateralis.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"105-8"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The characterization of Trypanosoma cruzi stocks by starch-gel electrophoresis, comparison of results with those of isoelectric focusing. 用淀粉凝胶电泳法对克氏锥虫原体进行鉴定，并与等电聚焦法比较。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
U Zillmann, F Ebert
{"title":"The characterization of Trypanosoma cruzi stocks by starch-gel electrophoresis, comparison of results with those of isoelectric focusing.","authors":"U Zillmann,&nbsp;F Ebert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The culture forms of 30 T. cruzi stocks originating from different parts of Brazil and Venezuela were screened by starch-gel electrophoresis for the isoenzyme patterns of 9 enzymes: glucose-6-phosphate dehydrogenase (G6PD), phosphoglucomutase (PGM), glucosephosphate isomerase (GPI), aspartate aminotransferase (ASAT), \"malic\" enzyme (ME), alanine aminotransferase (ALAT), peptidases (PEP 1, PEP 2) and malate dehydrogenase (MDH). G6PD, PGM, GPI to some extent also ASAT, ME patterns divide 25 stocks into two distinct main groups A and B. The Brazilian stocks of group A originated from different wild animal species only, those of group B were with one exception isolated from human patients, or domestic hosts. In contrast all stocks from Venezuela regardless of their original host belonged to group A. The results of all enzymes showed group A (16 stocks and 7 profiles) to be more homogeneous than group B (9 stocks and 7 profiles). Comparison of these results with previous results of isoelectric focusing of the same stocks shows both methods to be complementary. The two methods gave identical classification of stocks with G6PD, PGM and GPI, both methods dividing most stocks into two main groups. The enzymes ALAT and ASAT confirmed this grouping to some extent in the starch-gel electrophoresis. For both starch-gel and isoelectric focusing ME was of only limited value because of variable enzyme activities in the trypanosome extracts. For the enzymes PEP 1, and PEP 2 and MDH neither method was useful in demonstrating intraspecific variation within the T. cruzi complex.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"84-8"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17287610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of Onchocerca volvulus from cryopreserved microfilariae in three temperate species of laboratory-reared blackflies. 三种温养黑蝇低温保存微丝发育成扭转盘尾丝虫。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
P J Ham, A E Bianco
{"title":"Development of Onchocerca volvulus from cryopreserved microfilariae in three temperate species of laboratory-reared blackflies.","authors":"P J Ham,&nbsp;A E Bianco","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three species of British blackflies, Simulium ornatum s.l., S. erythrocephalum and S. lineatum, were infected with the cryopreserved microfilariae of Onchocerca volvulus obtained from human skin-snips in the Sudan. Doses of 5 or 10 microfilariae per fly were administered by intrathoracic injection into females, 1-2 days after eclosion from pupae. After 7 days at 27.5 degrees C and 85% relative humidity, microfilariae had completed development to third-stage larvae. Fly survival rates were highest for S. ornatum (96%) and lowest for S. lineatum (56%), and fell only marginally in each species when the larger dose of microfilariae was given. Infection rates ranged from 42% in S. lineatum up to 58% in S. ornatum following the lower dose of microfilariae, and 58% in S. lineatum up to 79% in S. ornatum following the higher dose. The proportion of microfilariae which completed development was relatively constant in each species of fly, ranging from 3.1-4.2% in S. lineatum to 10.5-16.8% in S. erythrocephalum. The greatest number of third-stage larvae recovered came from S. erythrocephalum at the higher dose of microfilariae, with a mean of 2.4 larvae per infected fly. As S. erythrocephalum has been successfully colonized through several generations in the laboratory, it is concluded that this is a promising species for reselection for increased susceptibility to O. volvulus.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"137-9"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trypanosoma brucei brucei infection in goats. Response of peripheral blood lymphocytes to mitogen stimulation. 山羊布鲁氏锥虫感染。外周血淋巴细胞对有丝分裂原刺激的反应。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
L Diesing, J S Ahmed, E Zweygarth, F Hörchner
{"title":"Trypanosoma brucei brucei infection in goats. Response of peripheral blood lymphocytes to mitogen stimulation.","authors":"L Diesing,&nbsp;J S Ahmed,&nbsp;E Zweygarth,&nbsp;F Hörchner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The in vitro proliferative response of peripheral blood lymphocytes from Trypanosoma b. brucei infected goats to mitogens was studied during the course of infection. An inverse correlation was observed between the number of lymphocytes and their capacity to respond to concanavalin A, phytohemagglutinin and pokeweed mitogen. Lymphocyte responsiveness to mitogens was reduced during a period of increased lymphocyte counts. During this period of time only few trypanosomes were detectable in the peripheral blood. Later in the infection, when lymphocytes decreased, mitogen responsiveness and parasitemia increased.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"79-83"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17930488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The fine structure of adult Onchocerca volvulus IV. The hypodermal chords of the female worm. 成虫盘尾丝虫的精细结构。雌虫的皮下索。
Tropenmedizin und Parasitologie Pub Date : 1983-06-01
M Franz, D W Büttner
{"title":"The fine structure of adult Onchocerca volvulus IV. The hypodermal chords of the female worm.","authors":"M Franz,&nbsp;D W Büttner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The morphology of the hypodermal chords in the anterior, midbody and posterior region and in worms of different ages are described and the amphids, the cephalic papillae and the nerve ring are demonstrated. At the anterior end, the hypodermal cap comprises the sensory organs. Close behind, the hypodermal chords are differentiated, and several cell margins are found in all of them. Four sublateral chords are differentiated, and several cell margins are found in all of them. Four sublateral chords are also found in the anterior region. A row of median cells is found between the dorsal and ventral syncytia further behind and at the posterior end. In the midbody region, the lateral chords are very large but without central cells. Various particles and inclusions such as bacteria, dense bodies and fibrils are found in the plasm of the hypodermal chords. A large multilayered inclusion was found in the degenerating chord of a female worm at least five years old.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 2","pages":"122-8"},"PeriodicalIF":0.0,"publicationDate":"1983-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17370677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructural aspects of intraerythrocytic development of a Venezuelan strain of Anaplasma marginale. 委内瑞拉边缘无原体菌株红细胞内发育的超微结构方面。
Tropenmedizin und Parasitologie Pub Date : 1983-03-01
S Giardina, A Bretaña, Q Márquez
{"title":"Ultrastructural aspects of intraerythrocytic development of a Venezuelan strain of Anaplasma marginale.","authors":"S Giardina,&nbsp;A Bretaña,&nbsp;Q Márquez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Blood samples from splenectomized calves infected with a Venezuelan strain of Anaplasma marginale were studied by electron microscope. A. marginale appears to invade erythrocytes by the invagination of the host cell plasma membrane. The parasite reproduces by binary or multiple fission, changing from a rounded to an irregularly polyhedral form during and after division. The mature Anaplasma, within the host red cell modifies the erythrocytes cytoplasma and external membrane producing pores through which the parasite can escape without lysing the host erythrocyte.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 1","pages":"7-10"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17896260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effects of diethylcarbamazine citrate, antihistamines and corticosteroid derivatives on skin reactivity to Onchocerca supernatants. 柠檬酸二乙基卡马嗪、抗组胺药和皮质类固醇衍生物对盘尾丝虫上清皮肤反应性的影响。
Tropenmedizin und Parasitologie Pub Date : 1983-03-01
V P Titanji, E Barla, S Anyangwe, J L Ngu
{"title":"The effects of diethylcarbamazine citrate, antihistamines and corticosteroid derivatives on skin reactivity to Onchocerca supernatants.","authors":"V P Titanji,&nbsp;E Barla,&nbsp;S Anyangwe,&nbsp;J L Ngu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A single dose of promethazine or of betamethazone, either alone or compounded with dexchloropheniramine, was found to inhibit skin wheal formation in onchocerciasis patients challenged with Onchocerca supernatants. The mean wheal diameter developing 24 hours after drug administration ranged between 40-60% less than the original pre-treatment diameter but this effect had been abolished or was significantly less by 48 hours. Diethycarbamazinecitrate had only a 20% inhibitory effect on the wheal diameter of the skin reaction. It is concluded that antihistamines and corticosteroid derivates may interfere with the immunodiagnostic skin test for onchocerciasis based on skin reactivity to Onchocerca supernatants, unless measures are taken to ensure that these drugs are not consumed within the 48 hours preceding the skin test.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 1","pages":"30-2"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17200114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The survival of adult Litomosoides carinii transplanted into cotton rats previously injected with irradiated stage 3 larvae. 经辐照3期幼虫注入棉大鼠体内后成虫移植成虫的存活率。
Tropenmedizin und Parasitologie Pub Date : 1983-03-01
D M Storey, A S Al-Mukhtar
{"title":"The survival of adult Litomosoides carinii transplanted into cotton rats previously injected with irradiated stage 3 larvae.","authors":"D M Storey,&nbsp;A S Al-Mukhtar","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Adult Litomosoides carinii were surgically removed from the pleural cavities of normal cotton rats which had been infected 35 days previously by the inoculation of normal stage 3 larvae. Groups of 20 such worms were then transplanted into either the pleural cavities of other normal, uninfected cotton rats or into the pleural cavities of cotton rats which had previously received, by subcutaneous injection, either 3 X 50 L3 which had been exposed to 40 krads Cobalt 60 irradiation or 1 X 50 L3 irradiated at 16.2 krads. Cotton rats vaccinated with irradiated L3 prior to transplant accepted transferred worms for at least 65 days whereas uninfected cotton rats soon encapsulated and destroyed the transferred worms. Microfilariae were detected in the pleural exudate and peripheral blood of vaccinated cotton rats but not in that of unvaccinated cotton rats.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 1","pages":"24-6"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17402758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ultrastructure of Mansonella ozzardi microfilaria, with a comparison of the South American (simuliid-transmitted) and the Caribbean (culicoid-transmitted) forms. Mansonella ozzardi微丝虫的超微结构，与南美(拟虫传播)和加勒比(库蚊传播)形式的比较。
Tropenmedizin und Parasitologie Pub Date : 1983-03-01
W J Kozek, C Raccurt
{"title":"Ultrastructure of Mansonella ozzardi microfilaria, with a comparison of the South American (simuliid-transmitted) and the Caribbean (culicoid-transmitted) forms.","authors":"W J Kozek,&nbsp;C Raccurt","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ultrastructural studies conducted to elucidate and compare the microanatomy of the South American (simuliid-transmitted) and the Caribbean (culicoid-transmitted) microfilariae of Mansonella ozzardi revealed that these microfilariae are essentially identical. The only difference detected was the larger central body of the Colombian microfilariae. Although the microanatomy of M. ozzardi microfilaria is essentially the same as that of other microfilariae, some of the subtle differences noted between M. ozzardi and other microfilariae include: amphids of unequal lengths, a large nucleus located approximately 30 microns from the anterior end, and an esophagus which appears to be less developed than that of Loa loa and Brugia spp. The microfilaria has a small cephalic hook, intracellular bacteriae in the hypodermal cells, and crystalloid inclusions which are most prominent in the R-1 cell and are also present in the hypodermal and esophageal cells. These crystalloids appear to be unique to M. ozzardi microfilariae.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 1","pages":"38-53"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17402759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The molluscicidal properties of Ambrosia maritima L. (compositae). 1. Design for a molluscicide field trial. 海棠(菊科)的杀螺性能。1. 杀软体剂田间试验的设计。
Tropenmedizin und Parasitologie Pub Date : 1983-03-01
M F El Sawy, J Duncan, T F Marshall, H K Bassiouny, M A Shehata
{"title":"The molluscicidal properties of Ambrosia maritima L. (compositae). 1. Design for a molluscicide field trial.","authors":"M F El Sawy,&nbsp;J Duncan,&nbsp;T F Marshall,&nbsp;H K Bassiouny,&nbsp;M A Shehata","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The composite plant, Ambrosia maritima is toxic to the snail intermediate hosts of schistosomiasis. A field trial was required to confirm this activity. A preliminary survey of irrigation canals and drains was conducted using two snail sampling methods. The results have been used to indicate the minimum number of watercourses which would have to be treated and the number of sampling stations per watercourse required to establish statistical significance in a kill of snails obtained from a molluscicide treatment.</p>","PeriodicalId":76764,"journal":{"name":"Tropenmedizin und Parasitologie","volume":"34 1","pages":"11-4"},"PeriodicalIF":0.0,"publicationDate":"1983-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17896327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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