V A Gvozdev, V T Kakpakov, L M Mukhovatova, L Y Polukarova, V Z Tarantul
{"title":"Influence of ecdysterone on the growth of cells and synthesis of macromolecules in established cell lines of Drosophila melanogaster.","authors":"V A Gvozdev, V T Kakpakov, L M Mukhovatova, L Y Polukarova, V Z Tarantul","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ecdysterone (beta-ecdysone) and 2-deoxy-alpha-ecdysone specifically inhibit the growth of established embryonic cells of D. melanogaster. A preparation of alpha-ecdysone is 100 times less active than ecdysterone. The action of ecdysterone is eliminated when it is removed within 24 h, but after 48 h the inhibiting influence of the hormone becomes irreversible. The diploid line, triploid and tetraploid sublines are sensitive to ecdysterone. The diploid subline with a spontaneously arisen translocation of an X-chromosome to an autosome of the third pair is substantially more resistant to ecdysterone. At 24 h after the addition of ecdysterone, there is a 2-5-fold suppression of the synthesis of total RNA; DNA synthesis is lowered to the same degree only after 48 h. The gross synthesis of proteins, measured according to the incorporation of C 14-lysine, was not suppressed even after 48 h.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"29-36"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11990433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of the synchronism of cleavage divisions.","authors":"A K Dondua, G K Dondua","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A method is described for estimating the degree of asynchronism of cell divisions in individual embryos at early developmental stages by means of a relative measure of asynchronism.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"19-23"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12293468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of antimycin A on sensivitivy of fertilized sea urchin egg cells to embryotoxic analogs of serotonin.","authors":"G A Buznikov, L N Markova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Antimycin A blocks cleavage divisions in concentrations 1-10-15-1-10-14 g/ml (experiments on Arbacia lixula) or 1-10-11-1-10-9 g/ml (experiments on sea urchins of the genus Strongylocentrotus). Subthreshold and threshold concentrations of this antibiotic do not affect the sensitivity of fertilized Strongylocentrotus intermedius to 1-benzyl-2, 5-dimethylserotonin, but increase their sensitivity to 5-butyltryptamine by a factor of 3-5. These data provide the basis for a discussion of the possible nature of the reception of serotonin and other low-molecular-weight regulators of early embryogenesis.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"63-5"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12345447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The problem of the origin of fibroblasts in the postnatal ontogenesis of mammals.","authors":"N G Khrushchov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An analysis is made of the author's data and the literature information on the origin and patterns of differentiation of the fibroblasts of connective tissue during the postnatal ontogenesis of mammals. The hypothesis of the existence of two types of collagen-producing cells (fibroblasts) - protective-trophic (short-lived population) and supporting (long-lived population) - is substantiated. Hypotheses are advanced on the biological role of these types of fibroblasts and their origin in phylogenesis.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12269614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship between respiration rate and weight of loach oocytes.","authors":"N D Ozernyuk, A I Zotin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It is shown that the constant k in the equation QO2 equals apk and the constant b in the equation qo2 equals aP-b change during the oogenesis of the loach. Hence, the growth of oocytes differs considerably from the growth of animals, where the constants k and b do not change with increase in weight. It is suggested that the relationship between the respiration rate and weight of the oocytes is due to the change in the amount of mitochondria in the oocytes.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"60-2"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12344994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Proliferation and growth of intestinal epithelium in Ascaris suum (Nematoda) during postnatal ontogeny. Communication III. Mitotic anomalies and changes in ploidy and nucleus size.","authors":"A P Anisimov, N P Tokmakova, L N Usheva","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was established by cytophotometry that the amount of DNA in intestinal-epithelium cells rises from the diploid to a tetraploid level during the early postnatal ontogeny of ascarids so that eventually nuclei with a diploid quantity of DNA practically disappear. Apart from this, there appear cells with a higher DNA content: 8c, 16c, 32c, and even 64c. Pictures of anomalous mitotic processes, as well as polypoid meta- and anaphases, were also observed. A result of the polyploidization is an increased size of nuclei and cells, but nucleus size is not always correlated with the DNA content. The previously established sixfold increase in the mean volume of intestinal cells during postnatal ontogeny of the ascarid is due to their transition to the new ploidy level and additional \"paragenomic\" growth of the polypoid cells.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12293470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relative degree of endopolyploidization of silk-gland cells in diploid and triploid silkworms.","authors":"V V Klimenko, T L Spiridonova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Photometric measurements showed that DNA content of the cells in the posterior portion of the silk gland to be higher in triploid caterpillars than in diploids. The somatic polyploidy level of posterior silk gland cells therefore increases as the number of cells in the organ decreases with rising overall organism ploidy.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"74-7"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12293473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth and respiration of regenerating tissues of the axolotl tail.","authors":"I G Vladimirova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Changes in the weight and oxygen consumption were studied during regeneration of the tail in adult axolotls and larvae. The curve of the increase in weight of the regenerating tail in both age groups is S-shaped. The intensity of respiration of the regenerating tail increases in adult axolotls and in larvae at the blastema stage; in adult axolotls there is also a second increase in the intensity of respiration of the regenerating tail during differentiation of the muscles. The relationship between weight and the rate of respiration was compared during regeneration of the tail in axolotl and the normal growth of the animals. Whereas growth of the animals was characterized by the relationship QO2 equals aPk with a constant value of k, during regeneration the various stages of this process have their own corresponding values of k.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"88-91"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12293476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New data on the hereditary adaptive increase in thermostability in the forked mutant of Drosophila melanogaster under repeated thermal shock.","authors":"P G Svetlov, G F Korsakova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>It was confirmed that forked mutants of D. melanogaster strain C(I)RM developing after a single intrauterine heating of oocytes in females at 39 degrees for 30 and 45 min exhibited changes in the average number of anomalous thoracic macrochaetae on females: heating for 30 min caused a decrease (normalization of the phenotype), while heating for 45 min caused an increase (intensification of mutation expression). It was established that these changes are inherited maternally, in the same manner as long-term Jollos modifications. Evaluation of the number of anomalous thoracic macrochaetae in parallel strains of flies after heating of the oocytes during the critical periods of macrochaeta development (seven days before egg laying), i.e., the stage of oocyte formation in the germarium. Heating of oocytes in F2t1 females \"preliminarily heated in the F1 generation showed that the stability curve for the oocytes after \"preliminary\" heating during the critical developmental period exhibited an abrupt decrease rather than the anticipated rise, which can only be attributed to an adaptive increase in oocyte thermostability as a result of preliminary heating.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"78-81"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11990434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N N Varakina, I V Zeleneva, R T Polikarpochkina, E E Khavkin
{"title":"Formation of the enzymatic apparatus of respiration in growing cells. Communication II. Reorganization of the respiratory cycle of mitochondria in the corn root tip.","authors":"N N Varakina, I V Zeleneva, R T Polikarpochkina, E E Khavkin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The content of cytochromes a,b and c, the activity of marker enzymes of the matrix and inner membrane of the mitochondria: glutamate dehydrogenase and cytochrome oxidase, as well as the rate of absorption of O2 by root segments in the presence of respiratory substrates, oxygen, inhibitors of respiration, and dinitrophenol, were determined. The intensification of cell respiration in the phase of elongation is determined not so much by new formation of cytochrome components of the respiratory cycle (during this period there is an accumulation only of cytochrome c) as by reorganization of the respiratory cycle (primarily its portion NADH - cytochrome b) and synthesis of enzymes of the matrix.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 1","pages":"52-9"},"PeriodicalIF":0.0,"publicationDate":"1975-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11381269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}