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The Soviet journal of developmental biology最新文献

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Distribution of neoblasts and mitoses during the asexual reproduction of the planarian Dugesia tigrina (Girard). 拟涡虫无性生殖过程中新母细胞和有丝分裂的分布。
The Soviet journal of developmental biology Pub Date : 1975-07-01
E B Krichinskaya, M G Martynova
{"title":"Distribution of neoblasts and mitoses during the asexual reproduction of the planarian Dugesia tigrina (Girard).","authors":"E B Krichinskaya,&nbsp;M G Martynova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The neoblast distribution in the prepharyngeal portion of the body remains unchanged during the asexual reproduction of planaria. During the first few days after fission, the number of neoblasts decreases in the portion of the body immediately adjoining the site of daughter zooid detachment and considerably increases in the regenerative bud. Starting with the fourth day after division, there is an increase in the number of neoblasts in the region of future fission, which can be regarded as one preparatory mechanism for the next fission. The mitosis distribution pattern is just the reverse: the regions of the body with the highest neoblast density are characterized by a low mitotic index or no dividing cells at all. The neoblast and mitosis distributions in the daughter zooid during its asexual reproduction cycle duplicate those observed in the maternal zooid.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"309-14"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12309378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incorporation of H-3-thymidine into mitochondrial DNA of quail oocytes and eggs. 鹌鹑卵母细胞及卵线粒体DNA中h -3-胸腺嘧啶的掺入。
The Soviet journal of developmental biology Pub Date : 1975-07-01
G V Dontsova
{"title":"Incorporation of H-3-thymidine into mitochondrial DNA of quail oocytes and eggs.","authors":"G V Dontsova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The amount of mitochondrial DNA and its synthesis were investigated in quail oocytes during the last 20 days of their growth. As the oocyte grows the amount of DNA in it increases rapidly, but at the end of oogenesis the increase in the amount of DNA lags considerably behind the rate of increase in the volume of the oocyte. As the oocyte grows the incorporation of H-3-thymidine into mitochondrial DNA decreases. The synthesis of mitochondrial DNA in cells of the quail embryo in early stages of development takes place at the same rate as the synthesis of nuclear DNA and far more rapidly than is more inhibited by ethidium bromide and mitomycin than the synthesis of nuclear DNA.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"364-7"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12309382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Change in myoglobin content in rabbit and chicken muscles in ontogenesis. 兔和鸡在个体发生过程中肌红蛋白含量的变化。
The Soviet journal of developmental biology Pub Date : 1975-07-01
P A Verbolovich, V V Mil'kanovich
{"title":"Change in myoglobin content in rabbit and chicken muscles in ontogenesis.","authors":"P A Verbolovich,&nbsp;V V Mil'kanovich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The accumulation of myoglobin in the muscles was studied in the ontogenesis of rabbits and chickens. A connection was found between the myoglobin content and the degree of the functional load of the muscles in different age periods. Myoglobin appears first in the myocardium. In the femoral muscles of chick embryos myoglobin is first found on the 16th day of incubation. Myoglobin is not detected quantitatively in the masseter and femoral muscles of the rabbit during prenatal development. The myoglobin content is highest in the gizzard of a mature hen.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"372-4"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12309383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The functional state of the thyroid gland of the mother and fetus in the prenatal development of rabbits. 兔产前发育过程中母胎甲状腺功能状态的研究。
The Soviet journal of developmental biology Pub Date : 1975-07-01
N S Salakhova, T A Kalyaeva, Y K Turakulov
{"title":"The functional state of the thyroid gland of the mother and fetus in the prenatal development of rabbits.","authors":"N S Salakhova,&nbsp;T A Kalyaeva,&nbsp;Y K Turakulov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The functional state of the thyroid gland of the mother and fetus was studied in different periods of intrauterine development of rabbits, as well as in newborn rabbits according to the level of protein-bound iodine (PBI) in the blood plasma and thyroid gland tissue. Similar studies were conducted after a thyroidectomy of females on the 10th-12th day of pregnancy in order to demonstrate the possibility of mutual compensation of the hormonal function under pathological conditions. The level of PBI in the blood plasma of the mother clearly increases in the second half of pregnancy and decreases sharply after birth. The content of PBI in the fetal blood plasma increases continuously beginning with the 22nd day of intrauterine development. The level of PBI in the thyroid gland tissue both of the mother and the fetus increases sharply at the end of pregnancy. In fetuses of thyroidectomized females the amount of PBI in the blood plasma and thyroid gland tissue on the 22nd day of pregnancy considerably exceeded that in normal fetuses.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"368-71"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11270878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of the splotch gene on the parameters of the cell cycle in the brain and spinal cord of mouse embryos. 斑点基因对小鼠胚胎脑和脊髓细胞周期参数的影响。
The Soviet journal of developmental biology Pub Date : 1975-07-01
B V Konyukhov, O V Mironova
{"title":"The effect of the splotch gene on the parameters of the cell cycle in the brain and spinal cord of mouse embryos.","authors":"B V Konyukhov,&nbsp;O V Mironova","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The parameters of the cell cycle were determined in 10-day-old mouse embryos, homozygous for the splotch (Sp) gene, by the radioautographic method with H-3-thymidine. Normal embryos (+/+)with a similar genetic environment were used as the control. In the brain and spinal cord of Sp/Sp embryos the mitotic indices were more than twice as high as those in +/+ embryos. The rate of cell proliferation increased identically in both the dorsal and ventral parts of the neural tube. The generation time of the brain and spinal cord cells of Sp/Sp embryos was 8 h and that of normal embryos 9 h. The Sp gene decreased the duration of the S phase 1.5 h (25 percent). In addition, this gene increased the duration of the G(1) phase 0.5 h. The Sp gene evidently acts in the G(1) phase, as a result of which the duration of this phase increases while that of the S phase decreases considerably. This leads to an increase in the rate of cell proliferation and to the development of abnormalities of the CNS of homozygous embryos.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"360-3"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12309381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RNA-polymerase activity in isolated nuclei of developing silkworm embryos. 家蚕胚离体细胞核rna聚合酶活性研究。
The Soviet journal of developmental biology Pub Date : 1975-07-01
P Kullyev, G G Gauze
{"title":"RNA-polymerase activity in isolated nuclei of developing silkworm embryos.","authors":"P Kullyev,&nbsp;G G Gauze","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nuclei isolated from developing embryos of the silkworm (Bombyx mori) are capable of RNA synthesis in vitro. The incorporation of C-14-uridine triphosphate (C-14-UTP) into the acid-insoluble fraction requires the presence of the other three ribonucleoside triphosphates; it is inhibited by actinomycin, olivomycin, and ethidium bromide, and is stimulated by ammonium sulfate. In a medium of moderate ionic strength C-14-UTP incorporation is slightly inhibited by alpha-amanitine (4 mug/ml), whereas in the presence of 0.2 M ammonium sulfate the same alpha-amanitine concentration reduces the incorporation by 90%. These data indicate that silkworm embryo nuclei, like nuclei from several other sources, contain two different RNA-polymerase fractions.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"350-2"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11270877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Incorporation of labeled amino acids into proteins of the cell organelles of muscle tissue during the restoration of the regenerative capacity of the limbs of axolotl, suppressed by x-irradiation. x射线抑制美西螈四肢再生能力恢复过程中，标记氨基酸与肌肉组织细胞器蛋白质的结合。
The Soviet journal of developmental biology Pub Date : 1975-07-01
N A Teplitz
{"title":"Incorporation of labeled amino acids into proteins of the cell organelles of muscle tissue during the restoration of the regenerative capacity of the limbs of axolotl, suppressed by x-irradiation.","authors":"N A Teplitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The incorporation of labeled amino acids into the total proteins of muscle tissue and cell organelles during the regeneration of limbs of axolotls and after the suppression of the regenerative capacity by x-irradiation and its experimental restoration were investigated. In the case of suppression of the regenerative capacity of the limbs by x-irradiation, the protein synthesis in the muscle tissue is impaired. The intensity of the incorporation of labeled amino acids into the proteins of a homogenate of muscle tissue, nuclei, mitochondria, and the mitochondria-free fraction is reduced in comparison with normal regeneration. Under the influence of biopreparations (a salt extract from a homogenate of the rat gastrocnemius muscle), a restoration of protein synthesis, processes of differentiation, and the formation of regenerates was observed. C-14-lysine was incorporated most intensively into the proteins of the mitochondria-free fraction and nuclei at the spatula stage (exceeding this index in the series with irradiation of the limbs without treatment with the biopreparation by 5 and 7-fold, respectively).</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"344-9"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12309379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Fertilization of the eggs of bivalve mollusks with different insemination conditions. 不同授精条件下双壳类软体动物卵的受精。
The Soviet journal of developmental biology Pub Date : 1975-07-01
Ginzburg
{"title":"Fertilization of the eggs of bivalve mollusks with different insemination conditions.","authors":"Ginzburg","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eggs of the bivalve mollusks Ostrea gigas, Spisula sachalinensis, and Mactra sulcataria taken from the ovary and placed in sea water are capable of maturation: the nuclear membrane dissolves and polar body I is released. The percentage of such spontaneously ripening eggs differs widely in different females and apparently characterizes the degree of roe maturity. The fertilization rate of roe containing a small percentage of spontaneously maturing eggs increases considerably during holding in sea water and remains at a high level for several hours. Maximum percentage fertilization can be obtained only over a narrow range of sperm concentrations in suspension for the species studied. A concentration of 10-6 or 10-7 sperm/cm-3 is usually optimum; a decrease or increase in sperm concentration by an order of magnitude (in comparison with the optimum level) reduces percentage fertilization. It was shown that sperm in suspensions with concentrations above the optimum level are activated to a lesser extent and their speed of movement is less than at high dilutions.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"300-8"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12309377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study of the nature of the growth-inhibiting factor in embryos of mice of the mutant line brachypodism-H. 短足- h突变系小鼠胚胎中生长抑制因子性质的研究。
The Soviet journal of developmental biology Pub Date : 1975-07-01
R S Bugrilova, B V Konyukhov, M V Pleskova, V M Rodionov
{"title":"Study of the nature of the growth-inhibiting factor in embryos of mice of the mutant line brachypodism-H.","authors":"R S Bugrilova,&nbsp;B V Konyukhov,&nbsp;M V Pleskova,&nbsp;V M Rodionov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>An extract from 13-day embryos of mice homozygous with respect to the bp-H gene contains a growth-inhibiting factor (GIF), which inhibits the growth of normal bones in vitro. The activity of the GIF depends on the concentration of the extract in the culture medium. The sensitivity of the GIF to heating and proteolytic enzymes is an indication of its protein nature. The precipitation of the GIF from the embryonic extract with ammonium sulfate, followed by fractionation on columns with Sephadex G-200 and DEAE-Sephadex gave a 400-fold degree of purification. The active growth-inhibiting preparation obtained is represented by three proteins, which migrate slowly in electrophoresis in polyacrylamide gel. The molecular weight of the basic component is 76,000.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"336-43"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12346750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Behavior of sperm nuclei injected into intact ripening and ripe toad oocytes and into oocytes ripening after removal of the germinal vesicle. 精子核注入完整的成熟和成熟的蟾蜍卵母细胞以及去除生殖囊泡后进入成熟的卵母细胞的行为。
The Soviet journal of developmental biology Pub Date : 1975-07-01
M N Skoblina
{"title":"Behavior of sperm nuclei injected into intact ripening and ripe toad oocytes and into oocytes ripening after removal of the germinal vesicle.","authors":"M N Skoblina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The behavior of frog sperm nuclei (obtained by treating a sperm suspension with pronase) in the cytoplasm of intact ripening and ripe toad oocytes and in the cytoplasm of oocytes ripening after removal of the germinal vesicle was studied. Sperm nuclei treated with pronase in the cytoplasm of intact ripe oocytes behave like the nuclei of sperm cells penetrating during normal fertilization: they increase in volume, synthesize DNA, and start to divide. They undergo no changes in the cytoplasm of oocytes ripening in the absence of karyoplasm. Transformations of sperm nuclei into pronuclei and DNA synthesis in them are found initially at the metaphase stage of the second maturation division.</p>","PeriodicalId":76730,"journal":{"name":"The Soviet journal of developmental biology","volume":"5 4","pages":"294-9"},"PeriodicalIF":0.0,"publicationDate":"1975-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12259093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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