在生长的细胞中形成的酶促呼吸装置沟通二世。玉米根尖线粒体呼吸循环的重组。

N N Varakina, I V Zeleneva, R T Polikarpochkina, E E Khavkin
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引用次数: 0

摘要

测定细胞色素a、b和c的含量,测定线粒体基质和内膜标记酶谷氨酸脱氢酶和细胞色素氧化酶的活性,测定呼吸底物、氧气、呼吸抑制剂和二硝基苯酚存在时根段对O2的吸收率。延长期细胞呼吸的增强与其说是由呼吸循环中细胞色素成分的新形成(在此期间只有细胞色素c的积累)决定,不如说是由呼吸循环的重组(主要是NADH -细胞色素b)和基质酶的合成决定的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Formation of the enzymatic apparatus of respiration in growing cells. Communication II. Reorganization of the respiratory cycle of mitochondria in the corn root tip.

The content of cytochromes a,b and c, the activity of marker enzymes of the matrix and inner membrane of the mitochondria: glutamate dehydrogenase and cytochrome oxidase, as well as the rate of absorption of O2 by root segments in the presence of respiratory substrates, oxygen, inhibitors of respiration, and dinitrophenol, were determined. The intensification of cell respiration in the phase of elongation is determined not so much by new formation of cytochrome components of the respiratory cycle (during this period there is an accumulation only of cytochrome c) as by reorganization of the respiratory cycle (primarily its portion NADH - cytochrome b) and synthesis of enzymes of the matrix.

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