The Kitasato archives of experimental medicine最新文献_第3页

Quantitative immunohistochemical distributions of tyrosine hydroxylase and calmodulin in the brains of spontaneously hypertensive rats. 自发性高血压大鼠脑组织中酪氨酸羟化酶和钙调素的定量免疫组化分布。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

K Akiyama, K Yabe, D Sutoo

{"title":"Quantitative immunohistochemical distributions of tyrosine hydroxylase and calmodulin in the brains of spontaneously hypertensive rats.","authors":"K Akiyama, K Yabe, D Sutoo","doi":"","DOIUrl":"","url":null,"abstract":"Immunohistochemical distributions of tyrosine hydroxylase and calmodulin in the rat forebrain were analyzed quantitatively as a possible model for the hypertension mechanism. The brain slices of spontaneously hypertensive rats (SHR) at 12 weeks of age were stained immunohistochemically for tyrosine hydroxylase and for calmodulin, and the distributions and amounts of these proteins were measured at 40-microns intervals by a fluorescence microphotometry system in comparison with those in normotensive control, Wistar Kyoto rats (WKY, the parent strain of SHR). Tyrosine hydroxylase levels in the neostriatum, nucleus accumbens, nucleus septi lateralis and tractus diagonalis, and calmodulin levels in the medial part of the neostriatum of SHR were lower than those in WKY. We reported previously that the decrease of the serum calcium level in SHR causes a decrease of the dopamine levels in the neostriatum and nucleus accumbens regions through a calmodulin-dependent system, and subsequent low levels of dopamine in the brain which may produce an increase in blood pressure. Combining this finding and our previous reports, we also suggest that the lower dopamine levels seen in the neostriatum and nucleus accumbens regions of SHR may result from the decrease in tyrosine hydroxylase and/or calmodulin levels in these regions in addition to the abnormality of calcium metabolism, and low levels of dopamine may produce an increase in blood pressure through functions of cerebral dopaminergic neurons and peripheral sympathetic nerves.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 4","pages":"199-208"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12536334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Symposium on Structure, Function and Product of Genes. The 5th Kitasato Research Conference. July 18, 1992. Abstracts. 基因的结构、功能和产物研讨会。第五届北中研究会议。1992年7月18日。摘要。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

引用次数: 0

Oxygen-independent antimicrobial activity against Salmonella enteritidis of specially activated macrophage with living vaccine. 特殊活化巨噬细胞活疫苗对肠炎沙门氏菌的氧不依赖性抗菌活性研究。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

T Sasahara, N Ikewaki, H Tamauchi, N Osawa

{"title":"Oxygen-independent antimicrobial activity against Salmonella enteritidis of specially activated macrophage with living vaccine.","authors":"T Sasahara, N Ikewaki, H Tamauchi, N Osawa","doi":"","DOIUrl":"","url":null,"abstract":"Characteristics of peritoneal macrophages recovered from mice infected with two attenuated strains SER and Jena of Salmonella enteritidis were compared. Strong resistance against lethal infection with a virulent strain 116-54 of S. enteritidis was seen in a group of mice immunized with strain SER, but not in a group of mice immunized with strain Jena as well as in a control group. Peritoneal macrophages from mice immunized with strain SER showed an enhanced Salmonella-killing activity, an increased generation of O2- and an increased expression of Ia antigen on 7 to 14 days after infection when compared with those from mice immunized with strain Jena and thioglycollate(TG)-elicited macrophages as a control. The bacterial number of strain Jena in organs decreased more rapidly than that of strain SER after day 4 of infection. These observations suggest that the survival of an attenuated Salmonella bacilli at reticulo-endothelium is essential to increase of their activities of macrophages. Macrophages from mice injected with recombinant interferon(IFN)-gamma for 3 days induced the activated stage of the same characteristics as noted in activated macrophages from mice immunized with strain SER. Effect of oxygen intermediates (OI) scavengers such as superoxide dismutase and catalase on Salmonella-killing activity of activated macrophages was not seen at all. These results suggest that an increased generation of OI may be not primarily responsible for the ability to inhibit the intracellular growth of a virulent strain of S. enteritidis in macrophages activated by immunization with live, attenuated strains and injection with rIFN-gamma.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 4","pages":"225-37"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12518020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular potentials of visceronociceptive neurons in posterior group of thalamic nuclei and its labeling with HRP intracellularly in cat. 猫丘脑核后组脏器感觉神经元的细胞内电位及其HRP标记。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

G Teng, X Meng, S Liu, X Ma

引用次数: 0

Cytotoxic effects of antitumor agents on mouse tracheal organ cultures at ultrastructural level. 抗肿瘤药物在超微结构水平上对小鼠气管器官培养的细胞毒作用。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

K Sekiya, Y Futaesaku, Y Nakase, H Danbara

{"title":"Cytotoxic effects of antitumor agents on mouse tracheal organ cultures at ultrastructural level.","authors":"K Sekiya, Y Futaesaku, Y Nakase, H Danbara","doi":"","DOIUrl":"","url":null,"abstract":"A cytotoxic effect of antitumor agents (mitomycin C;MMC, adriamycin;ADM, bleomycin;BLM, 5-fluorouracil;5-FU, and cisplatin;CDDP) on ciliated epithelial cells of mouse tracheal organ cultures was studied in transmission electron microscopy. Mouse tracheal rings incubated with each agent in a concentration of 1 microgram/ml or 10 micrograms/ml were observed after 1, 2, and 20 hr of the incubation. After 2 hr, the ciliated epithelial with MMC or ADM of 1 microgram/ml exhibited appearance of lipid droplets in the nonciliated cells and the swelling of mitochondria and epithelial cells. Further incubation of 20 hr with those agents resulted in the pronounced degeneration including the ciliary subsidence into intracellular spaces, balloon-like ciliary swelling, and cellular destruction. Whereas, the ciliated epithelia with 5-Fu, BLM or CDDP did not show any notable change within 2 hr. After 20 hr, these exhibited the swelling of mitochondria, cilia and epithelial cells. The ciliated epithelia incubated with MMC or ADM of a greater concentration of 10 micrograms/ml showed remarkable cytotoxic effects after 1 hr of the incubation. The morphological changes in the epithelial cells with 1 hr incubation were almost similar to those of 20 hr incubation with the 1 microgram/ml. After 20 hr, the cellular degeneration proceeded to extremely flattened epithelial cells with disappearance of cilia and appearance of numerous vacuoles. Those with 5-FU, BLM or CDDP of 10 micrograms/ml exhibited ciliary swelling after 2 hr, but the morphological changes of 5-FU were more remarkable than those of BLM or CDDP. After 20 hr, the pronounced degeneration was observed, and it was similar to one of MMC or ADM of 2 hr incubation.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 4","pages":"187-98"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12518018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of lipid peroxidation and antioxidants in aging process and thalassemia. 脂质过氧化和抗氧化剂在衰老过程和地中海贫血中的作用。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

N Chanarat

引用次数: 0

Suppression of 125I-uptake in mouse thyroid by seaweed feeding: possible preventative effect of dietary seaweed on internal radiation injury of the thyroid by radioactive iodine. 海藻喂养对小鼠甲状腺125i摄取的抑制:海藻对放射性碘对甲状腺内辐射损伤的可能预防作用。

The Kitasato archives of experimental medicine Pub Date : 1992-12-01

H Maruyama, I Yamamoto

{"title":"Suppression of 125I-uptake in mouse thyroid by seaweed feeding: possible preventative effect of dietary seaweed on internal radiation injury of the thyroid by radioactive iodine.","authors":"H Maruyama, I Yamamoto","doi":"","DOIUrl":"","url":null,"abstract":"We conducted an animal experiment to determine how dietary seaweeds rich in iodine and dietary fibers suppress radioactive iodine uptake by the thyroid, using mice and four kinds of experimental diets, three with 1% or 2% powdered fronds of the kelp Laminaria religiosa and 2% powdered laver Porphyra yezoensis, and one with cellulose. Iodine content of a hot-water extract of the kelp was 0.530 +/- 0.001%, and its dietary fiber (DF) values were 52.8 +/- 1.2%. Iodine in an extract of the laver was 0.008 +/- 0.001%, and its DF values were 41.4% +/- 0.7%. A statistically significant reduction of 125I uptake by the thyroid, 3 hours after intragastric administration of the radionuclide at a dosage of 18.5 kBq or 185 kBq in 0.3 ml aqueous solution per mouse, was observed in mice previously fed the experimental diets containing 1% and 2% kelp during periods varying from 24 hours to 7 days. The degree of the suppression was observed to depend on the amount of iodine in the diet or in the injected sample, no matter whether organic or inorganic, judging from the results of an additional experiment. Thus, we conclude that previously fed iodine-rich material, especially dietary seaweeds rich in iodine and other minerals, vitamins, and beta-carotene, such as kelps or laver supplemented with inorganic iodine, may be effective in prevention of internal radiation injury of the thyroid.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 4","pages":"209-16"},"PeriodicalIF":0.0,"publicationDate":"1992-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12518019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Depression of early protection against influenza virus infection by cyclophosphamide and its restoration by protein-bound polysaccharide. 环磷酰胺对流感病毒感染早期保护作用的抑制及蛋白结合多糖的恢复作用。

The Kitasato archives of experimental medicine Pub Date : 1992-09-01

S Tsuru

引用次数: 0

Higher sensitivity of the developing larvae of Angiostrongylus cantonensis than the adult worms to flubendazole and mebendazole. 发育中的广东管圆线虫幼虫对氟苯达唑和甲苯达唑的敏感性高于成虫。

The Kitasato archives of experimental medicine Pub Date : 1992-09-01

J Maki, S Kanda

{"title":"Higher sensitivity of the developing larvae of Angiostrongylus cantonensis than the adult worms to flubendazole and mebendazole.","authors":"J Maki, S Kanda","doi":"","DOIUrl":"","url":null,"abstract":"Two kinds of benzimidazoles, flubendazole and mebendazole were each administered at 10 mg/kg to rats harbouring the developing larvae of the rat lungworm, Angiostrongylus cantonensis 3 or 10 days post-infection and to those harbouring the adult worms 70 days post-infection. Almost all of the larvae were eliminated from the rats mediated 3 days post-infection. The larvicidal effects of the drugs administered 10 days post-infection were not so high as those 3 days post-infection. However, the growth of larvae in rats medicated 10 days post-infection were significantly inhibited as judged from their length, width and weight except the length of the larvae in rats given mebendazole. An inhibition of their growth was also demonstrated by the observation that no first-stage larvae were released from the rats medicated 10 days post-infection and examined 66 days post-infection at which the first-stage larvae were released from non-medicated rats. On the other hand, when the drugs were administered 70 days post-infection, no effects were seen on the number, body size and weight of recovered worms, and the release of the first-stage larvae. A sound conclusion was drawn that the developing larvae are more sensitive to the drugs than the adult worms.","PeriodicalId":76691,"journal":{"name":"The Kitasato archives of experimental medicine","volume":"65 2-3","pages":"131-6"},"PeriodicalIF":0.0,"publicationDate":"1992-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12483672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An occupational hygiene survey in a Chinese viscose rayon factory. 中国某粘胶人造丝工厂职业卫生调查。

The Kitasato archives of experimental medicine Pub Date : 1992-09-01

K Sugimoto, I Karai, S Goto, X J Gu, P K Lu, Z J Ding, M Q Li, Y X Liang, Y Seki

引用次数: 0